Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology | G3: Genes | Genomes...
Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology. Qisheng Zuo, Yinjie ... Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology. Qisheng Zuo, Yinjie ... Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology. Qisheng Zuo, Yinjie ... 2014 Genome-scale CRISPR-Cas9 knockout screening in human cells. Science 343(6166): 84-87. ...http://www.g3journal.org/content/6/6/1787
Conditional Knockout Mouse | Genome Engineering | genOway
Use conditional Knockout mice with tissue-specific or temporal gene inactivation to bypass embryonic lethality, compensatory ... Inducible Knockout mouse model. This strategy can bypass the limits that are usually observed with constitutive Knockout mouse ... Learn more about tissue-specific Knockout mouse models Learn more about inducible Knockout mouse models ... A conditional Knockout mouse defines an animal model in which a gene of interest can be:. * Inactivated in specific cell types ...https://www.genoway.com/services/customized-mouse/knockout-models/conditional-ko.htm
Inducible Knockout Mouse | Genome Engineering | genOway
Use an inducible Knockout mouse to inactivate your gene in an age-specific manner, and enable studies at defined development ... Inducible Knockout Mouse Model An inducible Knockout mouse defines a conditional biological model in which the target gene of ... This strategy can bypass the limits that are usually observed with conventional Knockout (constitutive KO) models (e.g. ... Custom Knockout Mouse Services at genOway. genOway provides a one-stop solution for the design, creation and analysis of your ...https://www.genoway.com/services/customized-mouse/knockout-models/conditional-ko-time.htm
Protein Function Knockout Mouse | Genome Engineering | genOway
A protein function Knockout mouse defines an model in which one or more nucleotides are mutated in a way that the protein ... Protein Function Knockout Mice (Point Mutations, Dead Kinase, etc.). Get supplemental information, a quote, and estimated ... Strengths and limitations of protein function Knockout mouse models. +. * Best way to reproduce human disease when due to ... A protein function Knockout mouse defines an animal model in which one or more nucleotides are mutated in a way that the ...https://www.genoway.com/services/customized-mouse/knockout-models/functional-ko.htm
Constitutive Knockout Rat Model | Genome Engineering | genOway
A constitutive Knockout rat defines a model in which the target gene is permanently inactivated in the whole animal, in every ... A constitutive Knockout rat, also referred to as conventional or whole-body Knockout (KO), defines a rat model in which the ... Constitutive Knockout Rat Models (Conventional). Get supplemental information, a quote, and estimated timeframe for generating ... Typical applications for constitutive Knockout rat models. For academic research: * Determine main functions of the gene and/or ...https://www.genoway.com/services/customized-rat/constitutive-knockout.htm
2009 News Feature: Making a Mightier Knockout Mouse - National Human Genome Research Institute (NHGRI)
To learn more about the international Knockout Mouse Project, go to The Knockout Mouse Project. To learn more about knockout ... Making a Mightier Knockout Mouse. A regular C57BL/6 mouse with black fur (top) sits next to a C57BL/6 knockout mouse (bottom) ... A Better Knockout Mouse. Still, the quest to create black 6 knockout mice continued because the 129 mouse strain displays ... To request any of the ES cell lines produced by the Knock Out Mouse Project, go to The Knock Out Mouse Project Repository, or ...https://www.genome.gov/27532436/making-a-mightier-knockout-mouse/
The mammalian gene function resource: the international knockout mouse consortium, Mammalian Genome | 10.1007/s00335-012-9422-2...
Mammalian Genome" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications ... A conditional knockout resource for genome-wide analysis of mouse gene function ... The knockout mouse project. Austin, CP; Battey, JF; Bradley, A; Bucan, M; Capecchi, M; Collins, FS; Dove, WF; Duyk, G; Dymecki ... The European dimension for the mouse genome mutagenesis program. Auwerx, J; Avner, P; Baldock, R; Ballabio, A; Balling, R; ...https://www.deepdyve.com/lp/springer-journals/the-mammalian-gene-function-resource-the-international-knockout-mouse-Ft86oUKCGG
Gadd45a knockout mice resemble p53 knockouts | Genome Biology | Full Text
The tumor suppressor gene p53 has been referred to as the 'guardian of the genome'. Mutations in the p53 gene are found in many ... The knockout mice also displayed increased numbers of cells in the thymus without evidence of decreased apoptosis. Finally, the ... Mice with a disrupted Gadd45a gene share several phenotypic characteristics with p53 knockout mice, including genomic ... supporting the notion that Gadd45a has a broad role in surveillance of genome stability. They refer to Gadd45a as an 'alarm ...https://0-genomebiology-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/gb-2000-1-1-reports002
TSG knock out in hiPSCs - EGA European Genome-Phenome Archive
The European Genome-phenome Archive (EGA) is part of the ELIXIR infrastructure.. EGA is an Elixir Core Data Resource. Learn ...https://ega-archive.org/studies/EGAS00001002262
Cell Based Assays / In vitro > Genetic Screens Laboratory Product Directory and Equipment Reviews on...
CRISPR Human Genome 80K Knockout Library (plasmid). Cellecta. Cellecta's 80K Single-module Human Genome-Wide CRISPR Library was ... designed to be small enough (~80,000 constructs) to enable convenient genome-wide "dropout viability" screens with most ...http://www.selectscience.net/cell-based-assays-+-in-vitro/product-directory/genetic-screens/?catID=1769&u=F9E1E535-116B-432B-B68F-932C8691DD2E
Application # 2017/0321212. SYSTEM FOR ISOLATING DNA MOLECULES - Patents.com
Genetic screens conducted with whole genome knockout collections such as the Yeast Knockout Collection (YKO)10 and the Keio ... In one implementation, the disclosed technology relates to a method for the construction of whole-genome knockout collections ... 0030] In one implementation, the disclosed technology can construct a curated, condensed, non-redundant whole-genome knockout ... whole-genome knockout collection. 8. The method of claim 7 wherein mutants of the progenitor collection catalog in singly- ...http://patents.com/us-20170321212.html
Addgene Blog: A Better Way to Share Science | Kendall Morgan
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Posted by Kendall Morgan on Feb 20, 2014 11:57:00 AM ... But what if you want to study genes all across the genome? Two new human lentiviral CRISPR library systems described in ... "Whole regions of the genome which were not accessible before are now accessible using this technology." ...http://blog.addgene.org/author/kendall-morgan/page/5
CRISPR-Cas9 Genome Editing
CRISPR/Cas9 is becoming a most popular genome editing tool due to its simplicity, with guide RNA (gRNA or crRNA and tracrRNA) ... GenCrispr Genome Cleavage Detection Kit. CRISPR gRNA Libraries In-stock GeCKO libraries for genome-wide knock-out; SAM ... CRISPR-Cas9 Genome Editing. Genome-editing technologies have brought revolutionary progresses in numerous fields of biology. ... Powerful Service Platform to Accelerate Your Genome Editing Research. CRISPR KO/KI for Mammalian Cell lines Fully-validated KO ...https://www.genscript.com/CRISPR-Cas9-Genome-Editing.html?src=pullmenu
Creation and Preliminary Characterization of Pregnane X Receptor and Constitutive Androstane Receptor Knockout Rats | Drug...
2016) Genome-wide analysis of human constitutive androstane receptor (CAR) transcriptome in wild-type and CAR-knockout HepaRG ... 2013) Whole-rat conditional gene knockout via genome editing. Nat Methods 10:638-640. ... Here, we report the creation and preliminary characterization of PXR and CAR knockout rats and PXR/CAR double knockout rats. ... knockout. PCN. pregnenlone-16α-carbonitrile. PCR. polymerase chain reaction. PXR. pregnane X receptor. P450. cytochrome P450. ...http://dmd.aspetjournals.org/content/45/10/1068.full
Eating at the Table of Another: Metabolomics of Host/Parasite Interactions
In the Apicomplexa, due to their haploid genomes, knockout parasite lines are particularly well-suited for metabolomic studies ... Genome Biol. 2006;7:R17. [PMC free article] [PubMed]. *Cho K, Shibato J, Agrawal GK, Jung YH, Kubo A, Jwa NS, Tamogami S, Satoh ... Genome Biol. 2005;6:R26. [PMC free article] [PubMed]. *May P, Wienkoop S, Kempa S, Usadel B, Christian N, Rupprecht J, Weiss J ... Genome Biol. 2005;6:R49. [PMC free article] [PubMed]. *Blume M, Rodriguez-Contreras D, Landfear S, Fleige T, Soldati-Favre D, ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2825149/?lang=en-ca
Linux Statistics Software - SourceForge.net
Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout 103 weekly downloads ...https://sourceforge.net/directory/science-engineering/mathematics/statistics/os:windows/os:linux/
Specific applications of CRISPR to genome-scale knockout screening and locus-specific epigenetic editing approaches will also ...http://www.asbmb.org/meeting2017/workshops/learninglabs/
Cellect Biotechnology Ltd Provides Corporate Update and Reports First Quarter 2017 Financi... ( TEL AVIV Israel May 29 2017 /-...
Launches a Single-Module 80K Human Genome-Wide CRISPR Knockout Library. 2. Sony Biotechnology Inc.s New Technology for Science ...http://www.bio-medicine.org/medicine-technology-1/Cellect-Biotechnology-Ltd-Provides-Corporate-Update-and-Reports-First-Quarter-2017-Financial-Results-57510-1/
BMC Bioinformatics | Networks analysis
Optimal knockout strategies in genome-scale metabolic networks using particle swarm optimization Knockout strategies, ... Multiple network algorithm for epigenetic modules via the integration of genome-wide DNA methylation and gene expression data ... A mixed-integer linear programming approach to the reduction of genome-scale metabolic networks Constraint-based analysis has ... While some of the associated algorithms can be applied to genome-scale network reconstructions with several thousands of r... ...https://bmcbioinformatics.biomedcentral.com/articles/sections/networks-analysis?searchType=journalSearch&sort=PubDate§ion=Networks+analysis&page=2
Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease | SpringerLink
Ascidian Ciona intestinalis Genome editing TALEN Knockout Electroporation This is a preview of subscription content, log in to ... In: Hatada I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. * DOI https ... Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly ... Satou Y, Mineta K, Ogasawara M et al (2008) Improved genome assembly and evidence-based global gene model set for the chordate ...https://link.springer.com/protocol/10.1007%2F978-1-4939-7128-2_19
Emerging evidence of the physiological role of hypoxia in mammary development and lactation | Journal of Animal Science and...
2003) . Because the whole-genome knockout of the HIF-1α gene is embryonic lethal, Seagroves et al. specifically removed the ... During this stage, estrogen and growth hormone regulate duct branching, as the knock-out of estrogen receptor (ER) α or growth ... The most compelling evidence of hypoxia's role in mammary development and lactation is from a targeted gene-knockout study by ... Prolactin is important for alveolar morphogenesis, as the knock-out of prolactin receptor or STAT5a, a major prolactin ...https://jasbsci.biomedcentral.com/articles/10.1186/2049-1891-5-9
Genomic Analysis </b> <...
Knockout Screening with Sanger Arrayed Genome-Wide CRISPR Libraries. May , 2016. Watch Webcast ... Presenters: David Segal, Ph.D, Professor of Biochemistry and Molecular Medicine, UC-Davis Genome Center; Jun Park, Ph.D, Lead ... Whether the focus is on engineering of new recombinant proteins or functional studies based on gene knock-out or knock-in, ... Presented are the first genome wide, arrayed guide RNA screening libraries for CRISPR-Cas9. These libraries have been designed ...http://www.merckmillipore.com/HK/en/support/support-library-webinars-videos-trainings/webinars/life-science-research/genomic-analysis/CRCb.qB.nPQAAAFGRPkPPoZw,nav
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source | Protocol
Shalem, O., et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 343, (6166), 84-87 (2014). ... An effector (the Cas9 nuclease or a nuclease-dead dCas9 fusion protein) is targeted to a specific site in the genome by a small ... Lane, A. B., et al. Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening. Dev Cell. 34, (3), 373-378 ( ... Koike-Yusa, H., Li, Y., Tan, E. P., Velasco-Herrera Mdel, C., Yusa, K. Genome-wide recessive genetic screening in mammalian ...https://www.jove.com/video/56264/a-universal-protocol-for-large-scale-grna-library-production-from-any
Sanger CRISPR Arrayed Library | China-Mainland | Sigma-Aldrich
The Power of Gene Knockout!. Two leaders in genome editing, MilliporeSigma and The Wellcome Sanger Institute, have joined ... The advent of pooled CRISPR libraries brought efficient genome-wide knockout but required the additional step of deep ... high-quality gene knockout and is available only at MilliporeSigma. Fast-track your research with the first whole genome ... Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes ...https://www.sigmaaldrich.com/china-mainland/zh/life-science/functional-genomics-and-rnai/sanger-crispr-arrayed-library.html
Squalene monooxygenase - Wikipedia
"A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337-42. doi:10.1038/ ... "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452-64. ... A conditional knockout mouse line called Sqletm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute. Male and ... Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11 ...https://en.wikipedia.org/wiki/Squalene_monooxygenase