Paul Sniegowski | Department of Biology
Genetic complementation analyses have shown that these high mutation rates are caused by defects in the MMR pathway. I am using ... Analysis of mutation rate data from fluctuation tests: Robust confidence intervals and hypothesis testing. Genetics, in ... Fluctuation test analysis programs (compiled for Windows) and source code from: Shaver, A.C. and P.D. Sniegowski. ... A test of the directed mutation hypothesis in Escherichia coli MCS2 using replica plating. Journal of Bacteriology 177:1119- ...https://www.bio.upenn.edu/people/paul-sniegowski
Adenoviral DNA replication: DNA sequences and enzymes required for initiation in vitro - CSHL Scientific Digital Repository
Adenoviruses Human Base Sequence Cell Nucleus DNA Viral DNA Directed DNA Polymerase Genes Viral Genetic Complementation Test ... dna dna polymerase radioisotope virus dna adenovirus dna replication dna sequence genetic engineering heredity nonhuman ...http://repository.cshl.edu/25106/
Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica).
Genetic Complementation Test. Light. Malus / genetics*, growth & development, metabolism. Molecular Sequence Data. Plant ... the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. ...http://www.biomedsearch.com/nih/Molecular-cloning-functional-analysis-blue/23314496.html
Complementation in Zellweger syndrome: biochemical analysis of newly generated peroxisomes.
Genetic Complementation Test. Humans. Microbodies / metabolism*, ultrastructure. Oxidation-Reduction. Zellweger Syndrome / ... Since this disorder is genetically heterogeneous and several complementation groups are known, we were able to study the ... normalization of peroxisomal activity during the process of complementation. The restoration of catalase and dihydroxyacetone ...http://www.biomedsearch.com/nih/Complementation-in-Zellweger-syndrome-biochemical/1511996.html
The CG12120 Gene Product Possesses NBAD Hydrolase and N | Open-i
Genetic Complementation Test. *Molecular Sequence Data. *Mutation. *Sequence Homology, Amino Acid. Related in: MedlinePlus ... To test whether CG12120 possesses these predicted activities, we produced recombinant CG12120 protein using a baculovirus ... To test whether CG12120 possesses these predicted activities, we produced recombinant CG12120 protein using a baculovirus ... This is, to our knowledge, the first molecular genetic characterization of NBAD hydrolase and carcinine hydrolase activity in ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC1285064_pgen.0010063.g004&req=4
Kinetics of SAM transport in E. coli. (A) Time course | Open-i
Genetic Complementation Test. *Kinetics. *Molecular Sequence Data. *S-Adenosylhomocysteine/metabolism. *Sequence Homology ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3104491_mbo0031111070002&req=4
3D-structural models of archaeal THI4 family proteins c | Open-i
Genetic Complementation Test. Related in: MedlinePlus. © Copyright Policy - open-access Related In: Results - Collection ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4215014_12866_2014_260_Fig2_HTML&req=4
Characterization of secreted HCV-LP produced in the pre | Open-i
Genetic Complementation Test. *Genotype. *Humans. *Microscopy, Electron, Transmission. *RNA, Viral/genetics. *Replicon ... Bottom Line: This closely resembled replicon-mediated NS2 trans-complementation, confirming that baculovirus expression of HCV ... Bottom Line: This closely resembled replicon-mediated NS2 trans-complementation, confirming that baculovirus expression of HCV ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4202265_068932-f5&req=4
ZFIN Publication: Brand et al., 1996
Genetic Complementation Test; Mesoderm/pathology; Motor Neurons/pathology; Mutation*; Nervous System/embryology; Zebrafish/ ... We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized ...http://zfin.org/ZDB-PUB-970210-11
B. bacteriovorus AspRS aspartylates tRNAAsn.Aminoacylat | Open-i
Genetic Complementation Test. *Kinetics. *Substrate Specificity. *Transfer RNA Aminoacylation. © Copyright Policy Related In: ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4206432_pone.0110842.g001&req=4
000646 - A/J
A/J inbred mice are widely used to model cancer and for carcinogen testing given their high susceptibility to carcinogen- ... Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further ... as confirmed by a genetic complementation test. Birth Defects Res A Clin Mol Teratol 76(8):574-9. PubMed: 16998816MGI: J:117594 ... In addition, a standard genetic test of allelism between clf1 and a Wnt9b targeted mutation demonstrated noncomplementation, ...https://www.jax.org/strain/000646
Pre-binding of small protein B to a stalled ribosome triggers trans-translation.
Blotting; Northern, Cell Division, Centrifugation; Density Gradient, Escherichia coli/metabolism, Genetic Complementation Test ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:102123
Protocols and Video Articles Authored by Joshua T. Gamse
Linkage analysis and genetic-complementation testing indicate that lor is an allele of tbx2b/fby (from beyond). fby was ... To test for developmental roles of scn8a, we knocked down Nav1.6a protein using antisense morpholinos. Na+ channel protein and ... To resolve the paradox that flhand tbx2b mutants have opposite phenotypes but have been placed in the same genetic pathway, we ... The zebrafish has proven to be a useful model to explore the genetic basis of neuroanatomical asymmetry in the developing ...https://www.jove.com/author/Joshua+T._Gamse
"In vitro CpG methylation increases the transformation efficiency of Bo" by Qiang Chen, Joshua Richard Fischer et al.
To test whether a methylation-sensitive restriction system contributes to poor B. burgdorferi transformability, shuttle ... The utility of in vitro methylation for the genetic manipulation of B. burgdorferi was exemplified by the ease of plasmid ... complementation of a B. burgdorferi B31 A3 BBK32 kanamycin-resistant (B31 A3 BBK32::Kan(r)) mutant, deficient in the expression ... as to hinder the genetic study of putative virulence factors. Two putative restriction-modification (R-M) systems, BBE02 ...https://escholarship.umassmed.edu/gsbs_sp/1402/
"Dominant negative mutator mutations in the mutS gene of Escherichia co" by Te-hui Wu and Martin G. Marinus
Genetic Complementation Test; Hydroxylamine; Hydroxylamines; Molecular Sequence Data; MutS DNA Mismatch-Binding Protein; * ... Mutagenesis; Nalidixic Acid; Nucleic Acid Heteroduplexes; Plasmids; *Point Mutation; Recombination, Genetic; Rifampin; Sequence ...https://escholarship.umassmed.edu/oapubs/663/
Rmr6 Maintains Meiotic Inheritance of Paramutant States in Zea mays | Genetics
Genetic complementation tests between the ems97406 mutation and other mutations known to affect Pl′ were conducted using +/ ... Stock constructions and complementation tests:. Hand pollinations were used for all genetic crosses and detailed pedigree ... Genetic tests demonstrate that Rmr6 encodes a common component required for establishing paramutations at diverse maize loci. ... To test the role of Rmr6 in r1 paramutation, near isogenic R-r/R-r and R-st/R-st W22 lines were first pollinated by a single +/ ...http://www.genetics.org/content/171/2/725
Rb deficiency during Drosophila eye development deregulates EMC, causing defects in the development of photoreceptors and cone...
Because d09015 is homozygous lethal, we performed a genetic complementation test to determine whether d09015 is an emc allele. ... To test this, we would need to generate double mutant clones of rbf1 and emc. However, because emc itself is required for ... Genetic screen and identification of d09015. To sustain high levels of UAS-transgene expression in both early and late stages ... 2A). We tested whether mis-expression of emc is capable of recapitulating the eye phenotype generated by d09015 by taking ...http://jcs.biologists.org/content/124/24/4203
Genetic Analysis and Gene Mapping. Genetic complementation tests and analysis of dominance were performed as described ... Genetic complementation analysis of all pairwise combinations of mutants was performed. Based on the thermotolerance phenotype ... and the new alleles were tested (before backcrossing) in many of the assays, as discussed in the text. Further genetic and ... thermotolerance was tested in all three assays, using the defined hot1-1 mutant as a reference. When tested for hypocotyl ...http://www.plantphysiol.org/content/132/2/757
Vacuolar precursor proteins undergo maturation after in | Open-i
Genetic Complementation Test. *Intracellular Membranes/enzymology/metabolism. *Micropore Filters. *Qa-SNARE Proteins ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199724_JCB9904110.f3&req=4
A single ataxia telangiectasia gene with a product similar to PI-3 kinase.
Genetic Complementation Test. *Genetic Predisposition to Disease. *Heterozygote. *Humans. *Male. *Meiosis. *Molecular Sequence ... ATM, which has a transcript of 12 kilobases, was found to be mutated in AT patients from all complementation groups, indicating ... The disease is genetically heterogeneous, with four complementation groups that have been suspected to represent different ...https://publications.ncats.nih.gov/publications/7792600
RT-PCR for fimA transcription. RT-PCR assays were used | Open-i
A complementation test revealed that transforming a recombinant plasmid possessing the coding sequence of a NAD(P)H-flavin ... Conclusion: Genetic determinants other than the fim genes may involve in the regulation of type 1 fimbrial expression in S. ... A complementation test revealed that transforming a recombinant plasmid possessing the coding sequence of a NAD(P)H-flavin ... Conclusion: Genetic determinants other than the fim genes may involve in the regulation of type 1 fimbrial expression in S. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2527010_1471-2180-8-126-3&req=4
Trans-complementation of ∆-NS1-WNV with ectopically e | Open-i
Trans-complementation of ∆-NS1-WNV with ectopically expressed WNV NS1. A. Scheme for construction of ∆-NS1-WNV. Nucleotides 87 ... Methods and results: Using a trans-complementation assay, we show that viruses deleted for NS1 (∆-NS1) can be rescued by ... Methods and results: Using a trans-complementation assay, we show that viruses deleted for NS1 (∆-NS1) can be rescued by ... Figure 1: Trans-complementation of ∆-NS1-WNV with ectopically expressed WNV NS1. A. Scheme for construction of ∆-NS1-WNV. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3842638_1743-422X-10-339-1&req=4
OPUS at UTS: Lateral Ftsz Association And The Assembly Of The Cytokinetic Z Ring In Bacteria - Open Publications of UTS Scholars
Genetic Complementation Test. en_US. dc.subject.mesh. Hot Temperature. en_US. ...https://opus.lib.uts.edu.au/handle/10453/8625
Inference of functional residues in anthocyanin transpo | Open-i
Genetic Complementation Test. *Models, Molecular. *Molecular Sequence Data. *Phylogeny. *Plants/classification/genetics ... VvGST4 also gave a similar level of complementation in the maize bombardment assay to these GSTs (Alfenito et al., 1998), ... VvGST4 also gave a similar level of complementation in the maize bombardment assay to these GSTs (Alfenito et al., 1998), ... The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2561157_jexbotern217f08_3c&req=4
Analysis of essential gene MTL50 values.Three curves fr | Open-i
Genetic Complementation Test. *Genome, Bacterial. *Microscopy, Fluorescence/methods. *Models, Biological. *Oligonucleotides, ... Significant difference (** = p,0.01, * = p,0.05) in MTL50 between genes was determined by a one-way ANOVA and Tukey HSD Test. ... Significant difference (** = p,0.01, * = p,0.05) in MTL50 between genes was determined by a one-way ANOVA and Tukey HSD Test. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2698124_pone.0006061.g006&req=4