Candidate Topical Microbicides Bind Herpes Simplex Virus Glycoprotein B and Prevent Viral Entry and Cell-to-Cell Spread
Limited variability of glycoprotein gene sequences and neutralizing targets in herpes simplex virus type 2 isolates and ... and the protein was coupled under the continuous flow of HBS-EP buffer (10 mM HEPES with 0.15 M NaCl, 3.4 mM EDTA, and 0.005% ... despite the structural dissimilarities between SAMMA and the SPs. Higher concentrations of CS were needed to achieve the same ... These results suggest that the primary mechanism of anti-HSV activity is inhibition of viral binding by targeting the viral ...http://pubmedcentralcanada.ca/pmcc/articles/PMC415621/?lang=en-ca
Togaviridae - Wikipedia
... forming four non-structural proteins responsible for gene expression and replication. The formation of a sub-genomic fragment, ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. "Togaviridae". ... The non-structural proteins are encoded at the 5' end, formed during the first of two characteristic rounds of translation. ... encoding the structural proteins and a negative sense fragment, a template for further synthesis of positive sense RNA are the ...https://en.wikipedia.org/wiki/Togaviridae
Semliki Forest virus - Wikipedia
The 5' two thirds of the genome encode four non-structural proteins concerned with RNA synthesis and the structural proteins ... Semliki Forest virus has been used extensively in biological research as a model of the viral life cycle and of viral ... Due to its broad host range and efficient replication, it has also been developed as a vector for genes encoding vaccines and ... Of the structural proteins, the C proteins makes up the icosahedral capsid which is enveloped by a lipid bilayer, derived from ...https://en.wikipedia.org/wiki/Semliki_Forest_virus
López-Giráldez F[au] - PubMed - NCBI
A locus encompassing the Epstein-Barr virus bglf4 kinase regulates expression of genes encoding viral structural proteins. ... Gene expression differences among three Neurospora species reveal genes required for sexual reproduction in Neurospora crassa. ... Global gene expression and focused knockout analysis reveals genes associated with fungal fruiting body development in ... Sex-specific gene expression during asexual development of Neurospora crassa.. Wang Z, Kin K, López-Giráldez F, Johannesson H, ...https://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=L%C3%B3pez-Gir%C3%A1ldez+F%5Bau%5D&dispmax=50
Jaagsiekte sheep retrovirus - Wikipedia
There are 4 genes that encode the viral structural proteins. They are "gag" encoding the structural internal virion proteins ... The viral proteins are synthesized initially as large precursors and are later processed into the mature proteins by ... An additional open reading frame (ORF) was observed in the viral genome and has been called orfX and its function is undefined ... But JSRV is different in this aspect since its envelope glycoprotein ("env") by itself is an oncogene and this single protein ...https://en.wikipedia.org/wiki/Jaagsiekte_sheep_retrovirus
Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia | PNAS
1B) (20). Transcription of HSV-1 LAT, but not the genes encoding the late viral structural proteins glycoprotein D and G, was ... A) Scatter plot showing the mean viral genome copy number per 105 TG cells. (B) Comparison of the viral copy numbers between ... A prominent viral factor could be LAT, shown to control latent viral infection by protecting neurons from undergoing apoptosis ... CD4 T-Cell Memory Responses to Viral Infections of Humans Show Pronounced Immunodominance Independent of Duration or Viral ...http://www.pnas.org/content/104/9/3496
Toxins | Free Full-Text | Vaccines against Botulism | HTML
... and recombinant protein-based vaccines. DNA-based vaccines include plasmids or viral vectors containing the gene encoding one ... Among the potential recombinant protein vaccines reviewed are HC, light chain-heavy chain translocation domain, and chemically ... of the BoNT heavy chain receptor binding domains (HC). Viral vectors reviewed are adenovirus, influenza virus, rabies virus, ... These vaccines include DNA-based, viral vector-based, ... replacing genes which encode for viral structural proteins and ...http://www.mdpi.com/2072-6651/9/9/268/htm
Ayman El-Guindy, PhD > Infectious Diseases | Pediatrics | Yale School of...
... the mechanisms that regulate expression of genes encoding viral structural proteins (e.g. capsid proteins), 2) The role of a ... long non-coding EBV RNA in promoting viral lytic replication and de novo infection, and 3) involvement of herpesviruses in the ...https://medicine.yale.edu/pediatrics/infectiousdiseases/people/ayman_el-guindy.profile
... gene have been maintained, whereas the genes coding for the viral structural proteins have been deleted and substituted by a ... An improved viral vector for cancer gene therapy should be capable of infecting tumors with high efficiency, inducing specific ... To combine safety and efficacy in cancer gene therapy, it is necessary for the therapeutic transgenic protein to be expressed ... In vitro and in vivo hepatoma cell-specific expression of a gene transferred with an adenoviral vector. Hum Gene Ther 1996; 7: ...http://cancerres.aacrjournals.org/content/66/3/1620
Viral and Cellular mRNA Translation in Coronavirus-Infected Cells
Other ORFs located downstream of the replicase gene encode viral structural proteins and a varying number of accessory proteins ... Following viral entry into the cell, the viral genome RNA undergoes translation to produce the viral proteins that are required ... in the envelope and contain a helical nucleocapsid that is formed by the viral genomic RNA and the viral N protein. The viral S ... frame within the nucleocapsid gene of mouse hepatitis virus encodes a structural protein that is not essential for viral ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5388242/
Pharmaceuticals | Free Full-Text | Anti-Viral Drugs for Human Adenoviruses | HTML
There are many stages in the development of a new drug for viral infection and such processes are even further complicated for ... the transcription of late virus genes which encode the viral structural proteins and proteins for the maturation of viral ... The treatment of such viral infections is not straightforward as with most viral infections and anti-viral research is faced ... One such strategy is to clone the binding sites for certain microRNAs to decrease the expression of viral genes. A study by ...http://www.mdpi.com/1424-8247/3/10/3343/htm
Antievolution.org - Antievolution.org Discussion Board -Topic::Science Break
Because of this lack of genes coding for structural proteins, it has been debated whether PDVs are of viral origin or a ' ... and it is surprising that it encodes almost no viral structural proteins, although it harbors immunosuppressive genes that are ... Because the DNA packaged in these particles encodes almost no viral structural proteins, their relation to viruses has been ... Further experiments showed that these genes code for key structural proteins in the wasps' polydnavirus toxins.. ...http://www.antievolution.org/cgi-bin/ikonboard/ikonboard.cgi?s=50aac4b75c629a1b
Development of dengue virus replicons expressing HIV-1 gp120 and other heterologous genes: a potential future tool for dual...
... we have attempted to create vectors which express dengue virus non-structural proteins and HIV immunogens. Previously we ... We cloned into a Δpre-M/E dengue virus replicon genes for either green fluorescent protein (GFP), HIV gp160 or HIV gp120 and ... Heterologous proteins were readily expressed from these constructs. GFP and gp120 demonstrated minimal or no toxicity. Gp160 ... reported the successful construction of dengue virus replicons which lack structural genes necessary for virion release and ...https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-1-28
Mutant Cellular AP-1 Proteins Promote Expression of a Subset of Epstein-Barr Virus Late Genes in the Absence of Lytic Viral DNA...
... proteins stimulated expression of a subgroup of late genes that encode viral structural proteins and immune modulators. In ... These findings indicate that newly synthesized viral DNA is not invariably required for viral late gene expression. While viral ... proteins. AP-1(A/S) activated 24 genes to high levels and 15 genes to intermediate levels; activation of 28 genes by AP-1(A/S) ... Mutant Cellular AP-1 Proteins Promote Expression of a Subset of Epstein-Barr Virus Late Genes in the Absence of Lytic Viral DNA ...https://jvi.asm.org/content/92/19/e01062-18
Filamentous Influenza Viruses | SpringerLink
... suggesting that structural variations in the M1 protein may govern viral structure . As the most abundant viral protein, M1 ... A single point mutation in a viral protein can alter morphology whereas silencing of a single cellular gene can also affect the ... These can broadly be categorised as encoding the viral structural or non-structural proteins. Haemagglutinin (HA), ... Whilst many studies have found an effect of cellular proteins and viral protein sequences on filament formation, the causation ...https://link.springer.com/article/10.1007%2Fs40588-016-0041-7
Recent Articles | Gene Therapy And Genetics & Genomics | The Scientist Magazine®| Page 5
One amino acid change within a viral structural protein makes the difference between mild cases of brain damage and severe ... Most Accurate CRISPR Gene Editing Yet. By Kerry Grens , September 22, 2017 ... CRISPR Used in Human Embryos to Probe Gene Function. By Ashley P. Taylor , September 20, 2017 ...https://www.the-scientist.com/?articles.list/tagNo/487,9/tags/gene-therapy,genetics--amp--genomics/pageNo/5/
Bacteriophage P2 : genes involved in baseplate assembly.
Viral Proteins/genetics/metabolism, Viral Structural Proteins/*genetics, Virus Assembly/*physiology Identifikatorer. URN: urn: ... Viral/analysis, Electrophoresis; Polyacrylamide Gel, Gene Expression Regulation; Viral, Genes; Viral/*genetics, Genome; Viral, ... Bacteriophage P2: genes involved in baseplate assembly.. Haggård-Ljungquist, Elisabeth Stockholms universitet, ... Molecular Sequence Data, Mutation, Rabbits, Recombinant Proteins, Research Support; Non-U.S. Gov't, Research Support; U.S. ...http://su.diva-portal.org/smash/record.jsf?faces-redirect=true&language=no&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&pid=diva2%3A178741&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity | Virology Journal ...
... these data provide biochemical evidence that I7L is a cysteine proteinase which is directly involved in VV core protein ... reaction conditions were developed which allowed accurate and efficient cleavage of exogenously added core protein precursors ( ... I7L gene product has been implicated as the major maturational proteinase required for viral core protein cleavage to occur ... The late genes encode many proteins required for the transcription of early genes, the viral structural proteins and the ...https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-63
Details and Download Full Text PDF: Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage...
Vpr, an accessory gene of human immunodeficiency virus type 1, encodes a virion-associated nuclear protein that plays an ... Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response.. Authors:. ... Background: Viral protein R (Vpr) is an accessory protein of HIV-1, which is potentially involved in the infection of ... The human immunodeficiency virus type 1 (HIV-1) protein Vpr (viral protein R) arrests cells in the G2 phase of the cell cycle, ...https://www.pubfacts.com/detail/29338752/Structural-alteration-of-DNA-induced-by-viral-protein-R-of-HIV-1-triggers-the-DNA-damage-response
Orthopoxvirus - Wikipedia
Late genes are expressed from 140 min to 48 hours post-infection, producing all viral structural proteins. Assembly of progeny ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the viral proteins to host ... Expression of early-phase genes by viral RNA polymerase begins at 30 minutes post-infection. The viral core is completely ... At this point, intermediate genes are expressed, triggering genomic DNA replication by the viral DNA polymerase at ...https://en.wikipedia.org/wiki/Orthopoxvirus
Patente US7507417 - Immunogenic compositions comprising human immunodeficiency virus type 2 (HIV ... - Google Patentes
... and three essential genes required for virus replication encoding the viral internal structural proteins (gag), the reverse ... In addition to these genes, both HIVs and SIV contain additional genes encoding the proteins that regulate viral expression ( ... This invention relates to viral proteins and glycoproteins, to compositions containing these proteins, to methods of preparing ... viral proteins in infected cells as well as in virus particles were studied. For comparison, the synthesis of HIV-1 proteins in ...http://www.google.es/patents/US7507417
Gene Report for G00001760 - Genes2Cognition Neuroscience Research Programme
... strongly enhancing and inhibiting viral structural protein synthesis, respectively. While investigating the inhibition of ... Gene lists (6). Databases (8). Curated Gene. OTTHUMG00000139859 (Vega human gene). Gene. ENSG00000070756 (Ensembl human gene). ... G2Cdb::Gene report. Gene id. G00001760. Gene symbol. PABPC1 (HGNC). Species. Homo sapiens. Description. poly(A) binding protein ... Gene lists (6). Gene List. Source. Species. Name. Description. Gene count. L00000009. G2C. Homo sapiens. Human PSD. Human ...http://www.genes2cognition.org/db/Gene/G00001760
Late genes encode mostly viral structural proteins or viral proteins necessary for the assembly and egress of the virus from ... VIRAL GENES NOT REQUIRED FOR VIRAL REPLICATION Viruses frequently have genes encoding proteins that are not directly involved ... including a viral matrix protein that coats the viral RNA. Viral RNA polymerase/integrase, matrix protein, and cellular tRNA ... Most early genes encode proteins that are necessary for viral DNA synthesis and for the turn-on of late-gene transcription. ...http://www.gkhub.info/medical-virology.html
HIV Infection and AIDS: Practice Essentials, Background, Pathophysiology
... the inner structural proteins. The pol gene encodes polymerase; it also contains integrase and protease (the viral enzymes) and ... The env gene encodes the viral envelope-the outer structural proteins responsible for cell-type specificity. Glycoprotein 120, ... Viral load in peripheral blood is used as a surrogate marker of viral replication rate; however, quantitative viral-load assays ... The accessory proteins of HIV-1 and HIV-2 are involved in viral replication and may play a role in the disease process. [21, 22 ...https://emedicine.medscape.com/article/211316-overview
Hepatitis C Virus Inhibits DNA Damage Repair through Reactive Oxygen and Nitrogen Species and by Interfering with the ATM-NBS1...
... mRNA expression and enhances NO production through the action of the viral structural protein core and nonstructural protein ( ... HTLV-I trans-activator protein, tax, is a trans-repressor of the human beta-polymerase gene. Science 247: 1082-1084. ... Some viral transforming proteins, such as the X protein of hepatitis B virus (12), the E6 protein of human papillomavirus (13, ... The viral core and nonstructural protein 3 proteins were shown to be responsible for the inhibition of DNA repair, mediated by ...http://www.jimmunol.org/content/185/11/6985.long