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Most recent papers with the keyword african swine fever | Read by QxMD
A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. ... Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p72 locus. Variation in the thymidine ... Both compounds displayed a potent, dose-dependent inhibitory effect on ASFV infection in Vero cells... ... Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells. ...https://www.readbyqxmd.com/keyword/78847
SMART: Secondary literature for RICIN domain
WT ricin and each of the single domain mutants was able to kill Vero cells following uptake by galactose containing receptors. ... In another line the RIP gene fused with a sugar binding (B-chain) gene to form the class of heterodimeric toxins. In some ... Following reassociation of these A chains to ricin B chain, Vero cells were challenged with these holotoxins and the ... In this paper, it is shown that the addition of A chain to mouse leukemia cells (AKR SL3) after the cells were first reacted ...http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=RICIN
Cas9 Nickase and Knocking out Gene Expression of Heme Oxygenase-1 in Vero Cells. Mary Agnes Cranley. Suren Ambegaokar (OWU ... Analysis of Cell Division and Development in C. elegans and Other Nematodes. Benjamin Whitbourn. Danielle Hamill (OWU ... Effects of Physiological Stress on HnRNP K Gene Expression in Drosophila Brain. Sydney Quinn. Suren Amegaokar (OWU Department ... Identifying Genotype-Dependent Expression of Genes near EoE Risk Loci. Madeline Bonfield. Leah Kottyan (Cincinnati Children's ...https://www.owu.edu/about/offices-services/academic-affairs/programs/summer-science-research-program/symposium-programs-and-student-research-abstracts/2017-abstracts/
Vero Cell Lines Expressing Nuclear Location Signals Of Penaeus Merguiensis Hepandensovirus: An Early Study
... cells in our study demonstrate no noticeable differences within transfected-Vero cell cultures with desired NLSs genes. The ... Vero cell lines expressing nuclear location signals of Penaeus merguiensis hepandensovirus: An early study. ... Densovirinae are intranuclear and require S-cells in their S-phase for all or most of all their replication and assembly. ... The presence of the NLS in the cell nucleus and cytoplasm was screened under a fluorescent microscope. Findings: All ...https://www.omicsonline.org/proceedings/vero-cell-lines-expressing-nuclear-location-signals-of-penaeus-merguiensis-hepandensovirus-an-early-study-66015.html
Toxins | Free Full-Text | Shiga Toxin: Expression, Distribution, and Its Role in the Environment | HTML
... toxin in the environment to work that demonstrates that the genetic mechanisms underlying the production of Shiga toxin genes ... are modified and embellished beyond the classical microbial gene regulatory paradigms in a manner that apparently ... For example, over 50 STEC strains have been isolated from deer feces and shown to contain stx genes and exhibit Vero cell ... of goats that contain microorganisms which possess a stx gene, some of which have been shown to be toxic to Vero cells, ...http://www.mdpi.com/2072-6651/3/6/608/htm
Department of Health | Shiga-Like Toxigenic <em>Escherichia coli</em> Laboratory Case...
A. Vero cell culture. Cell-free filtrates of enrichment broth cultures are applied to freshly split monolayers of Vero cells ... 2. Detection of Shiga (Vero) toxin genes in bloody faeces by a validated NA assay able to identify specific toxin sequences. ... Vero cell culture will allow detection of sorbitol fermenting STEC as well as O157. However this method is slow and only ... For Vero cell culture, a toxin-producing strain should be included in duplicate in each batch, together with a heat-treated ...http://www.health.gov.au/internet/main/publishing.nsf/Content/cda-phlncd-sltec.htm
Vero cell - Wikipedia
Research strains transfected with viral genes: Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein ... Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted ... Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. Vero E6 cells show some contact ... F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR ...https://en.wikipedia.org/wiki/Vero_cell
JCVI: Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line.
Passage of supernatants from microbat cells to Vero cells resulted in syncytium formation, and expression of viral genes and ... Next-generation sequencing and de novo assembly of the viral genome from supernatants from Vero cells yielded a single contig ... While employing deep sequencing and de novo assembly to characterize the mRNA transcript profile of a cell line derived from ... This virus can replicate in select human and nonhuman primate cell lines. However, analyses of viral sequences support a bat ...http://www.jcvi.org/cms/publications/listing/abstract/article/isolation-and-characterization-of-a-novel-gammaherpesvirus-from-a-microbat-cell-line-1/
METHOD FOR INFLUENZA VIRUS PURIFICATION - BIA SEPARATIONS D.O.O.
Sinai with deletion in NSl gene and IVR-116 was cultivated on Vero cells in Gibco™ OptiPRO™ SFM medium. The cell lysate was ... Sinai with deletion in NSl gene and IVR-116 was cultivated on Vero cells in Gibco™ OptiPRO™ SFM medium. The cell lysate was ... Sinai with deletion in NSl gene and IVR-116 was cultivated on Vero cells in Gibco™ OptiPRO™ SFM medium. The cell lysate was ... Sinai with deletion in NSl gene and IVR-116 was cultivated on Vero cells in Gibco™ OptiPRO™ SFM medium. The cell lysate was ...http://www.sumobrain.com/patents/wipo/Method-influenza-virus-purification/WO2008006780.html
Molecular cloning and expression of biologically-active diphtheria toxin receptor - Drawing for Patent # 5366874 -...
... library constructed from RNA of highly toxin-sensitive monkey Vero cells and with a neomycin resistance gene. One DT-sensitive ... about.1,000-fold more so than L-M cells and only .about.10-fold less than Vero cells) and are protected from DT toxicity on ... the cell surface receptors on DT.sup.S cells specifically bind radioiodinated DT, which is inhibited by unlabelled DT and the ... The purified DT.sup.S mouse cells are highly toxin-sensitive (. ... was rescued from DT.sup.S cells, and the screening procedure ...http://www.patentgenius.com/image/5366874-7.html
WO2002081709 GENES ET PROTEINES E6 ET E7 DU PVH MODIFIES POUVANT SERVIR A LA VACCINATION
Vero 2-2), and human (HeLa) origin. The cell line Vero 2-2 contains the HSV-1 IE2 (ICP27) gene and promoter. This line was ... Cells transfected to express HPV E6 and E7 genes and cell lines derived from cervical carcinomas infected by HPV, such as HeLa ... Figure 11: Western blot analysis of Vero cells transfected with pIN-EHBsAg-S-EE7T The cells were lysed 48 h after transfection ... EXAMPLE 2: Expression of EE7T fusion genes in mammalian cells. The expression of the EE7T fusion genes described in Example 1, ...https://patentscope.wipo.int/search/fr/detail.jsf
NDL India: Physiological Factors Of Growth And Susceptibility To Virus Regulating Vero Cells For Optimum Yield Of Vaccinia And...
Physiological Factors Of Growth And Susceptibility To Virus Regulating Vero Cells For Optimum Yield Of Vaccinia And Cloned Gene ... Physiological Factors Of Growth And Susceptibility To Virus Regulating Vero Cells For Optimum Yield Of Vaccinia And Cloned Gene ...https://ndl.iitkgp.ac.in/document/rEk4tkcWbgs90xKXVIfr6ivs2WZ7inqJKkchnnzYDvbOccV8cZqpuox62dAzrJToWSf3eify7Cw-BMlTjb8-Qw
Tracking Uptake, Assembly and Egress of ACAM529 Herpes Simplex Virus Type 2 in Complementary Vero Cells for Enhanced Virus...
The "ACAM529" strain is subject to two gene deletions and requires a complementary African Green Monkey Kidney (Vero) cell line ... Tracking Uptake, Assembly and Egress of ACAM529 Herpes Simplex Virus Type 2 in Complementary Vero Cells for Enhanced Virus ... Lastly, the AV529-19 Vero cells were shown to adhere effectively to insert membranes and form confluent monolayers. Staining ... The fluorescence which the HSV2012-121 Vero cells displayed on infection with ACAM529 did not allow for effective ...https://uwspace.uwaterloo.ca/handle/10012/10099
Welcome to HoPaCI
RT-PCR analyses showed that the dot/icm genes, dotB, icmQ, icmS, and icmW are expressed during C. burnetii infection of Vero ... cells. Formal Description. Interaction-ID: 32806 process. Coxiella burnetii infection increases_expression of ...http://mips.helmholtz-muenchen.de/HoPaCI/entry/show/3521?iList=32806&term=%26quot%3BCoxiella+burnetii+infection%26quot%3B&refFieldSingle=Biological+Process%28GO%29
Recombinant Varicella-Zoster Virus Vaccines as Platforms for Expression of Foreign Antigens
... gene, and SIV antigen expression in infected Vero cells was confirmed by immunofluorescence and immunoblot analysis using SIV ... A foreign gene of interest (goi) with promoter is inserted between ORFs 65 and 66. rVZV-BAC DNA is transfected into MeWo cells ... gene [14, 21, 22]. These rVZV-. viruses replicate as efficiently as wild-type VZV in cell culture. Generation of rVZV-. is ... Due to insertional inactivation of the viral TK gene, the rVZV replicated in infected MeWo cells in the presence of ...https://www.hindawi.com/journals/av/2013/219439/
Most recent papers with the keyword Pork | Read by QxMD
Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR ... Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells. ... read/29408315/genome-wide-analysis-of-differentially-expressed-genes-and-the-modulation-of-pedv-infection-in-vero-e6-cells ... An interesting trait of serovar I 4,,12:i:- is that it only expresses the fliC gene for bacterial motility (i.e., monophasic ...https://www.readbyqxmd.com/keyword/87633
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay | Protocol (Translated to Portuguese)
... um método para processar e tela de campo coletados mosquitos para uma diversidade de vírus por cultura de células Vero... ... Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti ... 3. Preparação de células Vero * Decantar meios de cultura de um grande frasco de cultura de tecidos (175 cm 2) de células Vero ... Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay. J. Vis. Exp. (52), e2889, doi:10.3791 ...https://www.jove.com/video/2889/deteco-de-vrus-infecciosos-de-campo-mosquitos-coletados-por-vero?language=Portuguese
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay | Protocol (Translated to Dutch)
... beschrijven een methode voor het verwerken en het scherm het veld verzameld muggen voor een diversiteit aan virussen door Vero ... Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti ... Detectie van infectieus virus van Field-Muggen verzameld door Vero Cell Culture Assay. Philip M. Armstrong1, Theodore G. ... Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay. J. Vis. Exp. (52), e2889, doi:10.3791 ...https://www.jove.com/video/2889/detectie-van-infectieus-virus-van-field-muggen-verzameld-door-vero?language=Dutch
The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function
2001). In fact, one of the T. brucei gp63 gene families is involved in the release of transgenic VSG from procyclic cells ( ... and antibodies against Tcgp63-I partially blocked the infection of Vero cells by trypomastigotes, which suggests a possible ... 1993) have demonstrated the presence of a gene from C. fasciculata with homology to the gp63 metalloprotease gene of Leishmania ... 1999). They may also play a pivotal role in cell-to-cell contact and recognition, as well as in the binding to the ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006&lng=en&tlng=en
A gene-targeted approach to investigate the intestinal butyrate-producing bacterialcommunity | Microbiome | Full Text
... and an almost complete absence of buk genes.Two patients were greatly enriched in buk genes similar to those ofClostridium ... In this study we describe a gene-targeted approach for 454 pyrotag sequencing andquantitative polymerase chain reaction for the ... in which there was a predominance of butgenes that are similar to reference genes from Acidaminococcus sp.,Eubacterium sp., ... final genes in the two primarybacterial butyrate synthesis pathways, butyryl-CoA:acetate CoA-transferase(but) and butyrate ...https://microbiomejournal.biomedcentral.com/articles/10.1186/2049-2618-1-8
NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in...
... was detected in immunoprecipitates from lysates of Vero cells transfected with plasmid DNA encoding a KUNV NS1-NS2A gene ... Our results in virus-infected or plasmid DNA-transfected cells indicate that the putative pseudoknot structure in the NS2A gene ... in the lysates of WT KUNV-infected cells but not in cells infected with the mutant viruses A30A′, FSSM, and A30P (Fig. (Fig.4B ... Fig.1B).1B). However, only NS1 and no NS1′ was detected in lysates of cells infected with the A30P mutant of KUNV. Further, no ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2812330/
The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation |...
Importantly, we show evidence for many proteins that match alternative gene models, or previously unpredicted genes. For ... Our data demonstrate how proteomics can inform these predictions and help discover new genes. We have identified nearly one- ... We also compared our data with existing transcriptional data in which we highlight apparent discrepancies between gene ... The draft genome for Toxoplasma predicts around 8,000 genes with varying degrees of confidence. ...https://genomebiology.biomedcentral.com/articles/10.1186/gb-2008-9-7-r116
SMART: Secondary literature for Aerolysin domain
Aerolysin is produced by E. coli cells containing the cloned aerolysin gene and it is processed normally by removal of the ... Eighty-nine percent of samples were cytotoxic in Vero cells. Our data demonstrated that Aeromonas spp. can harbor and express ... Incubation of cells with NADH or carnitine-enriched medium helped protect cells from toxin-induced ATP depletion and cell death ... The cells were also less sensitive to proaerolysin than wild type cells; however, transient transfection of FD11 cells with the ...http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=Aerolysin
Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks | BMC Microbiology | Full Text
The phylograms showed generally flat topologies among our strains' stx1 and stx2 genes. In the stx2 gene, 39.5% of the amino ... The stx1 and stx2 genes used in this phylogenetic study show sequence conservation with no significant divergence with respect ... and showed that there is generally more sequence variation among stx2 genes than stx1. ... The cytotoxic effects of Escherichia coli-produced Shiga toxin on Vero cells were first described 29 years ago . A few years ...https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-7-109
ORBi: Browsing ORBi
... we performed a series of transient expression assays in Vero cells, using a plasmid expressing ORF4 as effector and several VZV ... genes and heterologous genes as targets. The VZV target plasmids contained promoter/regulatory regions from genes belonging to ... We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and ... The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic ...http://orbi.ulg.ac.be/browse?type=author&value=Melen-Lamalle,%20Laurence%20p002363