The Role of Mitogen-activated Protein Kinase Activation in Determining Cellular Outcomes in Polyamine Analogue-treated Human...
cDNA Expression Array Analysis.. A cDNA macroarray system (Atlas; Clontech, Palo Alto, CA) was used to compare the relative ... we applied a commercially available cDNA expression array with 500 cancer-related genes. Comparisons were based on expression ... Purified cDNA probes (,6.5 × 106 cpm) were hybridized to the arrayed cancer cDNA membranes under recommended conditions. Washed ... Northern Blot Analysis.. Northern blot analysis was carried out as described by Fogel-Petrovic et al. (32) . Briefly, total RNA ...http://cancerres.aacrjournals.org/content/63/13/3619
VCY Gene - GeneCards | VCY1 Protein | VCY1 Antibody
... proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium ... Protein Coding), Variable Charge, Y-Linked, including: function, ... Protein array based interactome analysis of amyloid-β indicates ... The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) ... Entrez Gene Summary for VCY Gene. * The protein encoded by this gene is a member of a family of human VCX/Y genes. This gene ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=VCY
Patent US6905823 - Cellular arrays and methods of detecting and using genetic disorder markers - Google Patents
... providing copies of the array, and performing a different biological analysis of each copy. The results of the different ... analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder ... analyses are compared to determine if there are correlations between the results of the different biological analyses at each ... profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, ...http://www.google.com/patents/US6905823?dq=7,172,682
Enhanced Expression of RAD51 Associating Protein-1 Is Involved in the Growth of Intrahepatic Cholangiocarcinoma Cells |...
... out a genome-wide analysis of gene expression profiles of 25 ICCs by means of a cDNA microarray representing 27,648 genes (13 ... with the same aRNAs used for array analysis. We used a mixture of noncancerous intrahepatic biliary epithelial cells for the ... Gene silencing effect of RAD51AP1-si#11 was measured by Western blot analysis (top). Mutant RAD51AP1-siRNAs (mis#1, mis#2) ... Genome-wide analysis of gene expression in human intrahepatic cholangiocarcinoma. Hepatology 2005;41:1339-48. ...http://clincancerres.aacrjournals.org/content/14/5/1333
RNA and protein analyses.. Validation of array results and investigation of specific gene expression were carried out in RNA ... Starting from preliminary cDNA microarray analyses of human PBMC cultures exposed to recombinant TRAIL, which identified SOCS1 ... For Western blot analyses, PBMC samples were lysed as previously described (14). Equal amounts of protein for each sample were ... Levels of SOCS1 mRNA and protein were analyzed by quantitative RT-PCR (A and C) and Western blot (B) analyses, respectively. In ...http://diabetes.diabetesjournals.org/content/59/5/1261
Gene Expression Analysis of a Single or Few Cells - Current Protocols
Basic Protocol 2: Nonradioactive Gene Expression Analysis on Nylon Arrays. *Basic Protocol 3: Data Analysis of Hybridized cDNA ... binding protein 12 upregulation. Circulation. 103:1396‐1402. ... Basic Protocol 3: Data Analysis of Hybridized cDNA Arrays. ... Gene expression profiling of human stent‐induced neointima by cDNA array analysis of microscopic specimens retrieved by helix ... Basic Protocol 2: Nonradioactive Gene Expression Analysis on Nylon Arrays. Materials * Expand Long Template (ELT) PCR system ( ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-hg1108.html
C for RNA isolation and protein analysis.. RNA Isolation and Hybridization to Oligonucleotide Arrays. Total RNA was extracted ... Gene Ontology (GO) and pathway enrichment analyses were also performed within WPS for all the differentially expressed genes. ... cDNA Synthesis and Quantitation by Real-time PCR. Two micrograms of RNA were reverse transcribed into first strand using the ... Examples of these novel celecoxib-modulated genes identified in our study include genes such as protein kinases, ATM and mTOR/ ...http://cebp.aacrjournals.org/content/17/11/3051
... results of the whole genome cDNA micro-array and proteomic analyses indicated that EMF may activate several groups of genes ... computational biologist), studying protein functions, gene expression and genetic pathways. When I encountered an article ... Gene expression as well as protein expression and phosphorylation were also affected. "...the ... EMFs affect gene transcription, cause the synthesis of stress proteins and cause breakage of DNA, probably through generation ...http://www.freewebs.com/maggiezhou/wirelessharm.htm
Tumor-related interleukins: old validated targets for new anti-cancer drug development
Discovery of differentially expressed genes associated with paclitaxel resistance using cDNA array technology: analysis of ... Mitogen-activated protein kinase. MEK. Mitogen-activated protein kinase kinase. MMP. Matrix metalloproteinase. MPE2. ... Interleukin-15 in gene therapy of cancer. Current Gene Therapy. 2013;13:15. doi: 10.2174/156652313804806561. [PubMed] [Cross ... Interleukin 2 receptor gene expression in normal human T lymphocytes. Proc Natl Acad Sci U S A. 1985;82:6281. doi: 10.1073/pnas ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5606116/
Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea | Springer for...
Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of... ... Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially ... Northern blot analysis was used to confirm the cDNA array results.. Key words. Magnaporthe grisea Mature appressoria cDNA array ... A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was ...https://rd.springer.com/article/10.1631/jzus.2007.B0088
Gene Expression sub-cluster 37
We have shown by cDNA array analysis that SPARC upregulates membrane type 1-matrix metalloproteinase (MT1-MMP) and matrix ... To determine the metabolism location of the extra-cellular matrix proteins in fugu (Takifugu rubripes), we cloned the cDNAs of ... A disintegrin and metalloproteinases (ADAMs) are a new gene family of proteins with sequence similarity to the reprolysin ... Calreticulin is an endoplasmic reticulum protein important in cardiovascular development. Deletion of the calreticulin gene ...http://www.biomedsearch.com/cluster/10/Gene-Expression/sub-37-p12.html
Histology and analysis. Gene discovery using modified differential display, representational difference analysis, filter arrays ... In situ hybridisation and immunohistochemical analyses of milk protein and other gene expression in ruminants. ... Bovine lactating cDNA library. Bioactives from Milk (Histatherin, M-SAA3, Defensins, S100As. Recombinant gene expression. ... Microarrays, Western analysis. Epigenetic analysis (Sequenome, MeDIP, MBD). Udder ultrasound analyses. Early life nutrition. ...http://www.agresearch.co.nz/people/dr-adrian-molenaar-2/
AZD5153: A Novel Bivalent BET Bromodomain Inhibitor Highly Active against Hematologic Malignancies | Molecular Cancer...
Gene set enrichment analysis. Gene set enrichment analysis (GSEA; 16199517 and 12808457) was performed using the GSEA software ... Reverse phase protein array (RPPA) for proteomic profiling. Proteomic profiling was carried out using high-density protein dot ... PCR was performed using Taqman Gene Expression Master Mix (Applied Biosystems, cat#4369016) with 300 ng of cDNA and taqman ... Data are scaled according to z scores by protein. B, Western blot analysis of the change of protein level of select mTOR ...http://mct.aacrjournals.org/content/15/11/2563?utm_source=jan5spot&utm_medium=carousel&utm_campaign=0141
GPER promotes tamoxifen-resistance in ER+ breast cancer cells by reduced Bim proteins through MAPK/Erk-TRIM2 signaling axis
cDNA array analysis indicated a set of genes associated with cell apoptosis are aberrant in GPER activated and TAM-resistant ... Among these genes, Bim (also named BCL2-L11), a member of the BH3-only pro-apoptotic protein family is significantly decreased ... GPER promotes tamoxifen-resistance in ER+ breast cancer cells by reduced Bim proteins through MAPK/Erk-TRIM2 signaling axis. * ... By using immunohistochemical and western blot analysis, there is an adverse correlation between TRIM2 and Bim in TAM-resistant ...https://spandidos-publications.com/ijo/51/4/1191
ous analyses of protein sequences showed about half of. Pinus taeda ESTs on the array have an apparent homolog. in Arabidopsis ... C, Afshari C, Paules RS: Assessing gene significance from cDNA. microarray expression data via mixed models. j Comput Biol. ... cDNAs were estimated. The contig lengths constitute on. average about 45 % of the total cDNA lengths spotted on. the array. For ... array- and array*dye interaction variance lower than. 0.001 were used to select genes with significant changes in. abundance ( ...http://ufdc.ufl.edu/UF00100039/00001
Tiling array - Wikipedia
Tiling arrays provide an unbiased tool to investigate protein binding, gene expression and gene structure on a genome-wide ... For Affymetrix chips, the model-based analysis of tiling array (MAT) or hypergeometric analysis of tiling-arrays (HAT) are ... The method of sequencing cDNA to find transcribed genes also runs into problems, such as failing to detect rare or very short ... Another popular use of tiling arrays is in finding expressed genes. Traditional methods of gene prediction for annotation of ...https://en.wikipedia.org/wiki/Tiling_array
Gene and Protein Expression Profiles in a Mouse Model of Collagen-Induced Arthritis
We performed gene microarray and proteome array analyses using blood samples from the mice and found that 50 genes and 24 ... and 1 µg was used for cDNA synthesis with the SensiFAST cDNA Synthesis kit (Bioline, Taunton, MA, USA), with a primer annealing ... Decreased genes. No.. Gene symbol. Fold change. No.. Gene symbol. Fold change. No.. Gene symbol. Fold change. No.. Gene symbol ... The gene microarray and proteome array results were validated by evaluating the expression levels of select genes and proteins ...http://www.medsci.org/v15p0077.htm
Frontiers | Autoantibody Repertoire in APECED Patients Targets Two Distinct Subgroups of Proteins | Immunology
We here undertook a broad protein array approach to assess autoantibody repertoire in APECED patients. Our results show that ... APECED patients with deficient autoimmune regulator (AIRE) gene display a broad repertoire of high titer autoantibodies, ... We here undertook a broad protein array approach to assess autoantibody repertoire in APECED patients. Our results show that ... The APECED autoantigens have two major origins; proteins expressed in thymic medullary epithelial cells and proteins expressed ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00976/full
Degradomics - Wikipedia
... dedicated array for analysis of all protease, nonproteolytic homolog, and inhibitor gene transcripts in human and mouse. Curr. ... A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403,623- 627 (2000). Ito T., Chiba ... Again, total RNA is extracted and used to generate cDNA for PCR amplification. As long as there is a specific primer for a ... a microarray can handle parallel analysis of multiple genes while qRT-PCR must amplify one mRNA for analysis at a time. It also ...https://en.wikipedia.org/wiki/Degradomics
Identification of intrinsic and tissue-specific oncogenic pathways induced by Simian Virus 40 T/t-antigens: A network systems...
Microarray studies using the Incyte GEMII 10K cDNA array were performed on at least 5 different tumor samples (including ... When genes were examined for specific alterations in prostate adenocarcinoma, we identified a list comprising ∼1483 genes/ESTs ... A network systems analysis of expression profiles of mouse models of human cancers. Kristin Kee, Aleksandra M. Michalowska, ... and lung tumors were collected from Clara cell secretory protein CC10/Tag mice. ...http://cancerres.aacrjournals.org/content/66/8_Supplement/200.1
Mehrdad Shamloo | Stanford Medicine Profiles
Using hybridization, cloning techniques and cDNA array analysis to identify inducible neuroprotective genes, we found that ... We have focused our efforts on a subset of genes/proteins involved in neuroprotective or neurodegenerative pathways, which we ... K-means cluster analysis revealed two distinct biphasic gene expression patterns that contained 44 genes (including 18 ... the postischemic brain transcriptome was studied by a large-scale cDNA array analysis of three peri-infarct brain regions at ...https://med.stanford.edu/profiles/mehrdad-shamloo
Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes. | BMC Genomics |...
... miap-2 or miap-3 coding region cDNA probes [13, 14] Multiple phage isolates were arranged in a contiguous array by restriction ... Western blot analysis of the rat IAP proteins distribution in adult rat tissues. The protein samples prepared from fresh adult ... Deveraux QL, Reed JC: IAP family proteins-suppressors of apoptosis. Genes Dev. 1999, 13: 239-252.View ArticlePubMedGoogle ... While the function(s) of this testis-specific protein(s) is unknown at present the fact that both iap-1 and iap-2 genes are ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-3-5
Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB | The EMBO...
Analysis of enriched functional gene categories was carried out using PANTHER (Mi et al, 2013). Analysis of Matr3 intron length ... The array data were analysed using the ASPIRE3 pipeline. Only 61 genes showed changes in RNA levels of greater than twofold, ... Cross‐linked nucleotides are defined as the nucleotide upstream of mapped iCLIP cDNA tags as described before (König et al, ... Buratti E, Baralle FE (2012) TDP‐43: gumming up neurons through protein‐protein and protein‐RNA interactions. Trends Biochem ...http://emboj.embopress.org/content/early/2015/01/19/embj.201489852
RNASET2 - Wikipedia
An autoantigen in anaplastic large cell lymphoma identified by protein array analysis". J Proteomics. 75 (17): 5279-5292. doi: ... 2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome ... An autoantigen in anaplastic large cell lymphoma identified by protein array analysis". J Proteomics. 75 (17): 5279-5292. doi: ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...https://en.wikipedia.org/wiki/RNASET2
Significance analysis of microarrays - Wikipedia
Data from Oligo or cDNA arrays, SNP array, protein arrays,etc. can be utilized in SAM Correlates expression data to clinical ... Significant gene sets Positive gene set - higher expression of most genes in the gene set correlates with higher values of the ... Perform microarray experiments - DNA microarray with oligo and cDNA primers, SNP arrays, protein arrays, etc. Input Expression ... SAM identifies statistically significant genes by carrying out gene specific t-tests and computes a statistic dj for each gene ...https://en.wikipedia.org/wiki/Significance_analysis_of_microarrays
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