Comprehensive Gene Expression Analysis of Prostate Cancer Reveals Distinct Transcriptional Programs Associated with Metastatic...
In the other published analysis of metastatic tumors (12) , the hierarchical clustering of cDNA array data was used to identify ... 3 ⇓ ). We also performed immunohistochemical analysis of tumor samples and established that protein expression of some gene ... Gene expression analysis was carried out using Affymetrix U95 human gene arrays with 63,175 features for individual gene/EST ... Gene Expression Analysis Using Oligonucleotide Arrays.. We analyzed gene expression in prostate samples using hybridization of ...http://cancerres.aacrjournals.org/content/62/15/4499.full
The Role of Mitogen-activated Protein Kinase Activation in Determining Cellular Outcomes in Polyamine Analogue-treated Human...
cDNA Expression Array Analysis.. A cDNA macroarray system (Atlas; Clontech, Palo Alto, CA) was used to compare the relative ... we applied a commercially available cDNA expression array with 500 cancer-related genes. Comparisons were based on expression ... Purified cDNA probes (,6.5 × 106 cpm) were hybridized to the arrayed cancer cDNA membranes under recommended conditions. Washed ... Northern Blot Analysis.. Northern blot analysis was carried out as described by Fogel-Petrovic et al. (32) . Briefly, total RNA ...http://cancerres.aacrjournals.org/content/63/13/3619
Significance analysis of microarrays - Wikipedia
Data from Oligo or cDNA arrays, SNP array, protein arrays,etc. can be utilized in SAM Correlates expression data to clinical ... Significant gene sets Positive gene set - higher expression of most genes in the gene set correlates with higher values of the ... Perform microarray experiments - DNA microarray with oligo and cDNA primers, SNP arrays, protein arrays, etc. Input Expression ... SAM identifies statistically significant genes by carrying out gene specific t-tests and computes a statistic dj for each gene ...https://en.wikipedia.org/wiki/Significance_analysis_of_microarrays
Bayesian Modeling in Bioinformatics, Chapman & Hall/CRC Biostatistics Series by Dipak K. Dey | 9781420070170 | Booktopia
Sparsity Priors for Protein-Protein Interaction Predictions, I. Kim, Y. Liu, and H. Zhao Learning Bayesian Networks for Gene ... measurement error and survival models for cDNA microarrays, a Bayesian hidden Markov modeling approach for CGH array data, ... Bayesian approaches for phylogenic analysis, sparsity priors for protein-protein interaction predictions, and Bayesian networks ... The book explores Bayesian techniques and models for detecting differentially expressed genes, classifying differential gene ...https://www.booktopia.com.au/bayesian-modeling-in-bioinformatics-dipak-k-dey/prod9781420070170.html
Patent US6905823 - Cellular arrays and methods of detecting and using genetic disorder markers - Google Patents
... providing copies of the array, and performing a different biological analysis of each copy. The results of the different ... analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder ... analyses are compared to determine if there are correlations between the results of the different biological analyses at each ... profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, ...http://www.google.com/patents/US6905823?dq=7,172,682
Deciphering transcriptional networks that govern Coffea arabica seed development using combined cDNA array and real-time RT-PCR...
... the genes involved in the final chemical composition of the bean and the... ... Molecular cloning of the complete 11S seed storage protein gene of Coffea arabica and promoter analysis in transgenic tobacco ... targeted cDNA arrays, containing 266 selected candidate gene sequences, and real-time RT-PCR on a large subset of 111 genes. ... cDNA sequence, gene cloning and promoter analysis in transgenic tobacco plants. Plant Physiol Biochem 41:17-25CrossRefGoogle ...https://link.springer.com/article/10.1007%2Fs11103-007-9256-6
Enhanced Expression of RAD51 Associating Protein-1 Is Involved in the Growth of Intrahepatic Cholangiocarcinoma Cells |...
... out a genome-wide analysis of gene expression profiles of 25 ICCs by means of a cDNA microarray representing 27,648 genes (13 ... with the same aRNAs used for array analysis. We used a mixture of noncancerous intrahepatic biliary epithelial cells for the ... Gene silencing effect of RAD51AP1-si#11 was measured by Western blot analysis (top). Mutant RAD51AP1-siRNAs (mis#1, mis#2) ... Genome-wide analysis of gene expression in human intrahepatic cholangiocarcinoma. Hepatology 2005;41:1339-48. ...http://clincancerres.aacrjournals.org/content/14/5/1333
Table of Contents | Clinical Cancer Research
Discovery of Differentially Expressed Genes Associated with Paclitaxel Resistance Using cDNA Array Technology: Analysis of ... Expression of Nuclear Receptor Interacting Proteins TIF-1, SUG-1, Receptor Interacting Protein 140, and Corepressor SMRT in ... Phase I Study of an Antisense Oligonucleotide to Protein Kinase C-α (ISIS 3521/CGP 64128A ) in Patients with Cancer, ... Androgen Receptor Gene Alterations and Chromosomal Gains and Losses in Prostate Carcinomas Appearing During Finasteride ...http://clincancerres.aacrjournals.org/content/5/11
RNA and protein analyses.. Validation of array results and investigation of specific gene expression were carried out in RNA ... Starting from preliminary cDNA microarray analyses of human PBMC cultures exposed to recombinant TRAIL, which identified SOCS1 ... For Western blot analyses, PBMC samples were lysed as previously described (14). Equal amounts of protein for each sample were ... Levels of SOCS1 mRNA and protein were analyzed by quantitative RT-PCR (A and C) and Western blot (B) analyses, respectively. In ...http://diabetes.diabetesjournals.org/content/59/5/1261
Gene Expression Analysis of a Single or Few Cells - Current Protocols
Basic Protocol 2: Nonradioactive Gene Expression Analysis on Nylon Arrays. *Basic Protocol 3: Data Analysis of Hybridized cDNA ... binding protein 12 upregulation. Circulation. 103:1396‐1402. ... Basic Protocol 3: Data Analysis of Hybridized cDNA Arrays. ... Gene expression profiling of human stent‐induced neointima by cDNA array analysis of microscopic specimens retrieved by helix ... Basic Protocol 2: Nonradioactive Gene Expression Analysis on Nylon Arrays. Materials * Expand Long Template (ELT) PCR system ( ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-hg1108.html
VCY Gene - GeneCards | VCY1 Protein | VCY1 Antibody
... proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium ... Protein Coding), Variable Charge, Y-Linked, including: function, ... Protein array based interactome analysis of amyloid-β indicates ... The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) ... Entrez Gene Summary for VCY Gene. * The protein encoded by this gene is a member of a family of human VCX/Y genes. This gene ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=VCY
WikiGenes - ANG - angiogenin, ribonuclease, RNase A family, 5
The cDNA microarray analysis and protein array of secreted factors indicated angiogenin (ANG), an angiogenic factor, as a ... wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary ... 2. Analysis of the angiogenin (ANG) gene in the authors' population has demonstrated a significant allelic association with the ... Angiotensin II elevates nitric oxide synthase 3 expression and nitric oxide production via a mitogen-activated protein kinase ...http://www.wikigenes.org/e/gene/e/283.html
Genetic Analysis Assays | Thermo Fisher Scientific - AU
TaqMan Protein Assays. Quantitate protein expression using either predesigned TaqMan® Protein Assays for pluripotency markers ... We offer a wide array of simple and easy to use genetic analysis assays to meet the unique demands of translational and ... Highest-performing master mix for multiple real-time PCR applications, including microRNA and fast gene expression analysis. ... Since PCR requires a DNA template, RNA samples must first undergo reverse transcription to produce cDNA that can be used in ...https://www.thermofisher.com/au/en/home/clinical/clinical-translational-research/genetic-analysis/genetic-analysis-assays.html
... results of the whole genome cDNA micro-array and proteomic analyses indicated that EMF may activate several groups of genes ... computational biologist), studying protein functions, gene expression and genetic pathways. When I encountered an article ... Gene expression as well as protein expression and phosphorylation were also affected. "...the ... EMFs affect gene transcription, cause the synthesis of stress proteins and cause breakage of DNA, probably through generation ...http://www.freewebs.com/maggiezhou/wirelessharm.htm
Tumor-related interleukins: old validated targets for new anti-cancer drug development
Discovery of differentially expressed genes associated with paclitaxel resistance using cDNA array technology: analysis of ... Mitogen-activated protein kinase. MEK. Mitogen-activated protein kinase kinase. MMP. Matrix metalloproteinase. MPE2. ... Interleukin-15 in gene therapy of cancer. Current Gene Therapy. 2013;13:15. doi: 10.2174/156652313804806561. [PubMed] [Cross ... Interleukin 2 receptor gene expression in normal human T lymphocytes. Proc Natl Acad Sci U S A. 1985;82:6281. doi: 10.1073/pnas ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5606116/
Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea | Springer for...
Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of... ... Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially ... Northern blot analysis was used to confirm the cDNA array results.. Key words. Magnaporthe grisea Mature appressoria cDNA array ... A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was ...https://rd.springer.com/article/10.1631/jzus.2007.B0088
Gene Expression sub-cluster 37
We have shown by cDNA array analysis that SPARC upregulates membrane type 1-matrix metalloproteinase (MT1-MMP) and matrix ... To determine the metabolism location of the extra-cellular matrix proteins in fugu (Takifugu rubripes), we cloned the cDNAs of ... A disintegrin and metalloproteinases (ADAMs) are a new gene family of proteins with sequence similarity to the reprolysin ... Calreticulin is an endoplasmic reticulum protein important in cardiovascular development. Deletion of the calreticulin gene ...http://www.biomedsearch.com/cluster/10/Gene-Expression/sub-37-p12.html
A Kinetic Analysis of the Auxin Transcriptome Reveals Cell Wall Remodeling Proteins That Modulate Lateral Root Development in...
2002). Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30: 207-210. ... to ensure equal efficiency in the cDNA synthesis reactions between samples. qRT-PCR analysis using this cDNA was performed on ... Genes highlighted in yellow were identified in the PA analysis.. (B) A selection of clusters from the PA analysis reveals a ... A primary goal of this analysis was to identify genes whose protein products functioned in regulating growth and developmental ...http://www.plantcell.org/content/25/9/3329?ijkey=7ed7967aa0e3ff9ac9944500284420032a2655d5&keytype2=tf_ipsecsha
Histology and analysis. Gene discovery using modified differential display, representational difference analysis, filter arrays ... In situ hybridisation and immunohistochemical analyses of milk protein and other gene expression in ruminants. ... Bovine lactating cDNA library. Bioactives from Milk (Histatherin, M-SAA3, Defensins, S100As. Recombinant gene expression. ... Microarrays, Western analysis. Epigenetic analysis (Sequenome, MeDIP, MBD). Udder ultrasound analyses. Early life nutrition. ...http://www.agresearch.co.nz/people/dr-adrian-molenaar-2/
AZD5153: A Novel Bivalent BET Bromodomain Inhibitor Highly Active against Hematologic Malignancies | Molecular Cancer...
Gene set enrichment analysis. Gene set enrichment analysis (GSEA; 16199517 and 12808457) was performed using the GSEA software ... Reverse phase protein array (RPPA) for proteomic profiling. Proteomic profiling was carried out using high-density protein dot ... PCR was performed using Taqman Gene Expression Master Mix (Applied Biosystems, cat#4369016) with 300 ng of cDNA and taqman ... Data are scaled according to z scores by protein. B, Western blot analysis of the change of protein level of select mTOR ...http://mct.aacrjournals.org/content/15/11/2563?utm_source=jan5spot&utm_medium=carousel&utm_campaign=0141
C for RNA isolation and protein analysis.. RNA Isolation and Hybridization to Oligonucleotide Arrays. Total RNA was extracted ... Gene Ontology (GO) and pathway enrichment analyses were also performed within WPS for all the differentially expressed genes. ... cDNA Synthesis and Quantitation by Real-time PCR. Two micrograms of RNA were reverse transcribed into first strand using the ... Examples of these novel celecoxib-modulated genes identified in our study include genes such as protein kinases, ATM and mTOR/ ...http://cebp.aacrjournals.org/content/17/11/3051
Inhibition of Poly(ADP-Ribose) Polymerase Modulates Tumor-Related Gene Expression, Including Hypoxia-Inducible Factor-1...
Using cDNA expression array analysis, a substantial difference in key tumor-related gene expression was found between ... Real-time PCR gene expression and HIF-1α protein expression. We confirmed by real-time PCR the up-regulation of some of the ... Analysis of differential gene expression. We used cDNA microarrays to obtain gene expression patterns of treated skins during ... 19 genes up-regulated and 21 genes down-regulated in DPQ-treated mice; and 11 genes up-regulated and 20 genes down-regulated in ...http://cancerres.aacrjournals.org/content/66/11/5744?ijkey=c60ce04f2abf13d6f520c0827926dda4eb6802ae&keytype2=tf_ipsecsha
GPER promotes tamoxifen-resistance in ER+ breast cancer cells by reduced Bim proteins through MAPK/Erk-TRIM2 signaling axis
cDNA array analysis indicated a set of genes associated with cell apoptosis are aberrant in GPER activated and TAM-resistant ... Among these genes, Bim (also named BCL2-L11), a member of the BH3-only pro-apoptotic protein family is significantly decreased ... GPER promotes tamoxifen-resistance in ER+ breast cancer cells by reduced Bim proteins through MAPK/Erk-TRIM2 signaling axis. * ... By using immunohistochemical and western blot analysis, there is an adverse correlation between TRIM2 and Bim in TAM-resistant ...https://spandidos-publications.com/ijo/51/4/1191
Tiling array - Wikipedia
Tiling arrays provide an unbiased tool to investigate protein binding, gene expression and gene structure on a genome-wide ... For Affymetrix chips, the model-based analysis of tiling array (MAT) or hypergeometric analysis of tiling-arrays (HAT) are ... The method of sequencing cDNA to find transcribed genes also runs into problems, such as failing to detect rare or very short ... Another popular use of tiling arrays is in finding expressed genes. Traditional methods of gene prediction for annotation of ...https://en.wikipedia.org/wiki/Tiling_array
9/28/2004 REMBRANDT Empowering Translational Research… RE M BRA N DT REpository of Molecular BRAin Neoplasia DaTa HL7 Clinical...
... knowledge-base RNA 100K SNP array Protein Tissue Arrays for ISH Gene Expression Analysis Affy/Oligo Arrays cDNA/GenePix Arrays ... 13 9/28/2004 Gene Expression Search Use cases 14 9/28/2004 Gene Expression data analysis Binary chp files from GCOS Convert chp ... Conceptual Model Gene Expression Use Case analysis Gene Expression Data analysis Wire Frames System Architecture Object Model ... For example, in a gene expression query, the two dimensions needed are genes (GENE_DM) and samples (BIOSPECIMEN _DM) ...http://slideplayer.com/slide/717596/