*  High‐Throughput Single Nucleotide Polymorphisms Genotyping Technologies
Currently, the three next‐generation sequencing platforms are Illumina® Genome Analyzer, Roche® 454 GS‐FLX Sequencer and ABI® ... 2007) Power to detect risk alleles using genome‐wide tag SNP panels. PLoS Genetics 3: 1827-1837. ... International HapMap Consortium (2007) A second generation human haplotype map of over 3.1 million SNPs. Nature 449: 851-861. ... Mardis ER (2008) The impact of next‐generation sequencing technology on genetics. Trends in Genetics 24: 133-141. ...
  http://www.els.net/WileyCDA/ElsArticle/refId-a0021631.html
*  India Allele Finder: a web-based annotation tool for identifying common alleles in next-generation sequencing data of Indian...
India Allele Finder: a web-based annotation tool for identifying common alleles in next-generation sequencing data of Indian ... We present India Allele Finder (IAF), an online database table of allele frequencies of individuals from the Indian ... We built India Allele Finder, an online searchable database and command line tool, that gives researchers access to variant ... India Allele Finder offers improved ease-of-use to investigators seeking to identify and annotate sequencing data from Indian ...
  https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2556-2
*  Calculating Variant Allele Fraction of Structural Variation in Next Generation Sequencing by Maximum Likelihood
... ... "Calculating Variant Allele Fraction of Structural Variation in Next Generation Sequencing by Maximum Likelihood." (2015) ... I describe in this thesis a maximum likelihood approach to estimate variant allele fraction of a putative structural variation ... and found BreakDown outperformed other methods such as THetA in estimating variant allele fractions. ...
  https://scholarship.rice.edu/handle/1911/87870
*  Frontiers | Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele...
Identification of S-alleles in self-incompatible cultivars allowed allocating them in 11 incompatibility groups, six of them ... Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-( ... Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-( ... 2013b)] alleles in addition to the S6 allele mentioned above. Moreover, many of the S-alleles, such as S10-S30 alleles, in ...
  https://www.frontiersin.org/articles/10.3389/fpls.2018.00527/full
*  Epi-Alleles in Plants: Inheritance of Epigenetic Information over Generations : Plant and Cell Physiology - oi
... is often heritable over many generations. Such heritable properties allow conventional genetic linkage analysis to identify the ... is often heritable over many generations. Such heritable properties allow conventional genetic linkage analysis to identify the ...
  http://oxfordindex.oup.com/view/10.1093/pcp/pcf131
*  Microbiology Society Journals | Introgression in the genus Campylobacter: generation and spread of mosaic alleles
... was investigated by the examination of mosaic alleles, those with some ancestry from each population. A total of 1738 alleles ... Alleles with less than 75 % assignment probability to one of the populations were confirmed as mosaics using the structure ... For each of these, the putative source of the recombinant region was determined and the allele was mapped onto a clonalframe ... These observations confirm previous analyses of the exchange of complete housekeeping alleles and extend this work by ...
  http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.045153-0
*  DiVA - Search result
FIA obtained significant support for the halothane-associated QTL and identified the base generation allele with the mutated ... The estimated generation times in liquid culture medium for a subset of strains ranged from 5 to 22 hours, but did not ... This allows the user to decide the error tolerance level when inferring parental origin of the alleles. This is a novel feature ... This allows the user to decide the error tolerance level when inferring parental origin of the alleles. This is a novel feature ...
  http://uu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22categoryId%22%3A%2211558%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
*  Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele (pdf) | Paperity
Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele. September Generation of a pancreatic ... Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele, PLOS ONE, 2017, DOI: 10.1371/journal.pone ... Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele. PLOS ONE, Sep 2017 ... Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice Generation of primary tumors with Flp recombinase in ...
  http://paperity.org/p/84490354/generation-of-a-pancreatic-cancer-model-using-a-pdx1-flp-recombinase-knock-in-allele
*  Genetic Inheritance Exercise 1: F1 Hybrid Cross Results: Punnett Square
Generation Expected Alleles V Expected Alleles V P , dominant x recessive ______ _ppss_ F1 Progeny , _______ Frequency ... ð alleles , ¡ñ alleles v F1 Parent, genes:__________ (student fill in blank) 1. Expected phenotypic ratio of green to white ... 1 point) alleles eggs sperm colors 2. Mendel called plants that received different alleles for a trait from each parent ____. ( ... D. From the phenotype of the kernels on each P generation cob what would the predicted genotype of any F1 plant be?. E. Given ...
  https://www.jiskha.com/display.cgi?id=1303676564
*  Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with...
Generation of temperature-sensitive alleles of PpPex1p. The first step in identifying the cellular partners of PpPex1p was the ... generation of temperature-sensitive PpPEX1 alleles for use in a suppressor screen. The PpPEX1 ORF was subjected to a PCR-based ... Temperature-sensitive alleles of PpPEX1 were generated by a procedure described earlier (22). pJAH17 (11) is an autonomously ... Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC108805/
*  Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology | Genome Biology | Full Text
... and enables the rapid generation of both simple and complex alleles. ... The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient ... a Generation of knockout and knockin alleles using embryonic stem (ES) cell technology in mice. A cloning procedure is ... For the generation of knockout alleles, the microinjection of a single guide RNA (sgRNA) in zygotes is sufficient to create ...
  https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1409-1
*  Unique and Shared Functions of Nuclear Lamina LEM Domain Proteins in Drosophila | Genetics
Generation of bocks deletion alleles. The parental line used to generate bocks mutants was bocksCB03586, a line that carries a ... Generation and analysis of bocks deletion alleles. (A) Structure of the bocks locus, including a portion of the uncharacterized ... This strategy produced two bocks deletion alleles, bocksΔ10 and bocksΔ66. Deletions in both alleles extend 5′ from +11, with ... Deletion alleles of bocks were generated by imprecise deletion of a P element inserted at +11 in the bocksCB03586 allele ( ...
  http://www.genetics.org/content/197/2/653
*  Reporter Gene Insertions Reveal a Strictly B Lymphoid-Specific Expression Pattern of Pax5 in Support of Its B Cell Identity...
Generation of two Pax5 alleles with different reporter gene insertions. A, Structure of the targeted Pax5 alleles. The ... The Pax5ihCd2 and Pax5iGFP alleles were backcrossed for seven generations or more into the C57BL/6 genetic background. The Pax5 ... Generation of two Pax5 alleles with distinct reporter gene insertions. The expression of Pax5 was so far determined in cultured ... Similar expression of both Pax5 alleles throughout B cell development. To monitor the expression of each Pax5 allele, we ...
  http://www.jimmunol.org/content/178/5/3031
*  Intraflagellar transport is essential for endochondral bone formation | Development
Generation of a conditional allele of. Ift88 As Ift88 is expressed in most cell types throughout the mammalian body, and ... Generation of a conditional allele of Ift88. (A) Schematic representation of the targeting construct and the Ift88 genomic ... Overall, the generation of a conditional allele of the IFT protein Ift88 provides a valuable resource to allow the study of IFT ... B) Wild-type, floxed and null alleles (generated from the floxed allele) of Ift88 were distinguished by PCR using genomic DNA ...
  http://dev.biologists.org/content/134/2/307
*  Plus it
Generation of the Gabra5 floxed allele and the conditional KO mice. A replacement-type targeting vector (total genomic homology ... 1A, top). The floxed allele was bred to the C57BL/6J background (The Jackson Laboratory) for at least 13 generations and ... A, Generation of α5DGKO mice. P1-P3, PCR primers (loxP sites and primers not drawn to scale). B, False color image of DAB ... Generation of α5DGKO mice. The DG-selective reduction in α5-GABAAR expression (Fig. 1B-E′) was confirmed through ...
  http://www.jneurosci.org/content/35/40/13698
*  Biology-Online • View topic - Gene Frequency
Than your new allele frequencies (call them p1 and q1 for F1 generation) derived from this:. p1 = Frequency of AA + ½ Frequency ... Remember, the P generation dominant allele frequencies are 0.6 and 0.2.. (0.6 + 0.2) ÷ 2 = 0.4. (0.4 + 0.8) ÷ 2 = 0.6. So, it ... Genetic equilibrium means that the ratio of alleles is not changing between generations, but it has nothing to do with what ... In Mednel's punnet square, you give a 50%50 chance to each allele (two rows/columns; one for each allele). However, you're ...
  http://www.biology-online.org/biology-forum/about25997.html?p=144847&hilit=Chance
*  no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila | Development
Generation of the nopo-null allele. A nopo-null allele was generated by imprecise excision of P-element EYG5845. The 771-bp ... We generated a null allele of nopo (Exc142) via imprecise excision of EYG5845 (Fig. 2A; see Materials and methods). nopoExc142 ... A conserved residue in the RING domain is altered in our EMS-mutagenized allele of nopo, suggesting that E3 ligase activity is ... To gain further insights into the functions of nopo, we obtained additional alleles. 5-SZ-3004 (abbreviated as SZ3004) and ...
  http://dev.biologists.org/content/136/3/449
*  Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules | JCB
Generation of the klar null allele. To generate the klar null allele, FLP-mediated recombination was used between two PBac ... We used the alleles klarΔ1-18, which deletes the entire klar locus, and msp-300Δ3′, which deletes roughly half of the 3′ region ... Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Nat. Genet. 36:288-292. doi: ... S1 shows a scheme of MSP-300 and klar gene loci and the various alleles used in the present study. Fig. S2 shows confocal scans ...
  http://jcb.rupress.org/content/198/5/833.long
*  Dual Roles of Glutathione in Ecdysone Biosynthesis and Antioxidant Function During Larval Development in Drosophila | Genetics
Generation of Gclc loss-of-function mutant allele. Generation of the Gclc allele was carried out by the clustered regularly ... Generation of complete Gclc loss-of-function allele in D. melanogaster. We first generated a complete Gclc loss-of-function ... After these procedures, we eventually selected the Gclc46 allele. Since this allele has a large (46 bp) deletion in the coding ... In the next generation, the number of adult offspring males (Gclc46/Y with GAL4 and UAS) and females (Gclc46/+ with GAL4 and ...
  http://www.genetics.org/content/207/4/1519
*  Feedback regulation of Drosophila BMP signaling by the novel extracellular protein Larval Translucida | Development
Generation and molecular characterization of ltl alleles. The only identifiable protein domains within LTL are 13 LRRs (Fig. 3A ... 3B). Two early larval lethal alleles encoded premature stop codons (ltl1, G15* and ltl2, G455*), while two weaker alleles ... 3D). Additional alleles were obtained for which we could not define the lesion, including a putative protein null, ltlN (Fig. ... D) Western blot showing reduction of LTL protein levels in homozygous embryos of the hypomorphic alleles ltl3 and ltl4, and ...
  http://dev.biologists.org/content/138/4/715
*  Mechanisms of Evolution by Christopher Himmelheber on Prezi
A population in Hardy-Weinberg equilibrium has constant allele frequencies over generations. Conditions for Hardy-Weinberg. 1. ... May lead to formation of new alleles. New alleles may be adaptive ... Changes in allele frequency in a population*. Five main processes. Small Population (Genetic Drift). Non-Random Mating. ... Changes in allele frequency due to random chance. Much larger effect in small populations. Population Bottleneck. New ...
  https://prezi.com/v9yotxvmtjeo/mechanisms-of-evolution/
*  Anthro Exam 1 Quiz - By keldaleebee
change in frequency of alleles from one generation to the next. in a population, the percentage of all alleles at a locus ... expression of two alleles in heterozygotes, neither allele is dominant or recessive. ... allele frequencies are altered in small populations that are taken from, or are remnants of, larger populations. ... having same alleles at same locus on members of a pair of chromosomes (TT, tt). ...
  https://www.sporcle.com/games/keldaleebee/213exam1