India Allele Finder: a web-based annotation tool for identifying common alleles in next-generation sequencing data of Indian...
India Allele Finder: a web-based annotation tool for identifying common alleles in next-generation sequencing data of Indian ... We present India Allele Finder (IAF), an online database table of allele frequencies of individuals from the Indian ... We built India Allele Finder, an online searchable database and command line tool, that gives researchers access to variant ... India Allele Finder offers improved ease-of-use to investigators seeking to identify and annotate sequencing data from Indian ...https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2556-2
Calculating Variant Allele Fraction of Structural Variation in Next Generation Sequencing by Maximum Likelihood
... ... "Calculating Variant Allele Fraction of Structural Variation in Next Generation Sequencing by Maximum Likelihood." (2015) ... I describe in this thesis a maximum likelihood approach to estimate variant allele fraction of a putative structural variation ... and found BreakDown outperformed other methods such as THetA in estimating variant allele fractions. ...https://scholarship.rice.edu/handle/1911/87870
Frontiers | Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele...
Identification of S-alleles in self-incompatible cultivars allowed allocating them in 11 incompatibility groups, six of them ... Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-( ... Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-( ... 2013b)] alleles in addition to the S6 allele mentioned above. Moreover, many of the S-alleles, such as S10-S30 alleles, in ...https://www.frontiersin.org/articles/10.3389/fpls.2018.00527/full
Epi-Alleles in Plants: Inheritance of Epigenetic Information over Generations : Plant and Cell Physiology - oi
... is often heritable over many generations. Such heritable properties allow conventional genetic linkage analysis to identify the ... is often heritable over many generations. Such heritable properties allow conventional genetic linkage analysis to identify the ...http://oxfordindex.oup.com/view/10.1093/pcp/pcf131
"Generation of new Notch2 mutant alleles." by B McCright, J Lozier et al.
We previously described construction and analysis of a hypomorphic allele of the Notch2 gene. Homozygosity for this allele ... These new Notch2 mutant alleles expand the set of tools available for studying the myriad roles of the Notch pathway during ... We report here novel Notch2 mutant alleles generated by gene targeting in embryonic stem cells, including a conditional null ... allele in which exon 3 of the Notch2 gene is flanked by loxP sequences. ...https://mouseion.jax.org/stfb2000_2009/1282/
Microbiology Society Journals | Introgression in the genus Campylobacter: generation and spread of mosaic alleles
... was investigated by the examination of mosaic alleles, those with some ancestry from each population. A total of 1738 alleles ... Alleles with less than 75 % assignment probability to one of the populations were confirmed as mosaics using the structure ... For each of these, the putative source of the recombinant region was determined and the allele was mapped onto a clonalframe ... These observations confirm previous analyses of the exchange of complete housekeeping alleles and extend this work by ...http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.045153-0
Proof of concept studies to generate ES cell lines with c21orf2 conditional knockout allele and validate CRISPR generation of...
Proof of concept studies to generate ES cell lines with c21orf2 conditional knockout allele and validate CRISPR generation of ...https://researchportal.bath.ac.uk/en/projects/proof-of-concept-studies-to-generate-es-cell-lines-with-c21orf2-c
If S is a dominant allele and s is a recessive allele, what is the interpretation of the second generation offspring when Ss is...
If S is a dominant allele and s is a recessive allele, what is the interpretation of the second generation offspring when Ss is ... Answers for If S is a dominant allele and s is a recessive allele, what is the interpretation of the second generation ... However, if there are more than 1 type of genotype in the 1st offspring, I don't know how to continue it to the 2nd generation. ... and the 2nd generation offspring will be as simple as Ss crossed with Ss.. ...https://www.biology-questions-and-answers.com/if-s-is-a-dominant-allele-and-s-is-a-recessive-allele-what-is-the-interpretation-of-the-second-generation-offspring-when-ss-is-crossed-with-ss.html
"Generation of mice with a conditional null allele of the Jagged2 gene." by J Xu, L T. Krebs et al.
Cre-mediated deletion of the Jag2(flox) allele generates the Jag2(del2) allele, which behaves genetically as a Jag2 null allele ... Mice homozygous for a Jag2 null allele die at birth from cleft palate, precluding study of Jag2 function in postnatal and adult ... We have generated a Jag2 conditional null allele by flanking the first two exons of the Jag2 gene with loxP sites. ... This Jag2 conditional null allele will enable investigation of Jag2 function in a variety of tissue-specific contexts. ...https://mouseion.jax.org/stfb2010/97/
Cancer Biomarkers, Molecular Diagnostic Assays, Test Menu, Protein, Targets, SNPs, INDELs, Translocations, Next Generation...
Drug Diagnostics Codevelopment Strategies | MolecularMD MolecularMD.com devises the best codevelopment and commercialization strategy for drugs & diagnosticshttp://molecularmd.com/codevelopment-cdx-commercialization/pathways-to-commercialization/
DiVA - Search result
FIA obtained signiﬁcant support for the halothane-associated QTL and identiﬁed the base generation allele with the mutated ... The estimated generation times in liquid culture medium for a subset of strains ranged from 5 to 22 hours, but did not ... This allows the user to decide the error tolerance level when inferring parental origin of the alleles. This is a novel feature ... This allows the user to decide the error tolerance level when inferring parental origin of the alleles. This is a novel feature ...http://uu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22categoryId%22%3A%2211558%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele (pdf) | Paperity
Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele. September Generation of a pancreatic ... Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele, PLOS ONE, 2017, DOI: 10.1371/journal.pone ... Generation of a pancreatic cancer model using a Pdx1-Flp recombinase knock-in allele. PLOS ONE, Sep 2017 ... Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice Generation of primary tumors with Flp recombinase in ...http://paperity.org/p/84490354/generation-of-a-pancreatic-cancer-model-using-a-pdx1-flp-recombinase-knock-in-allele
Genetic Inheritance Exercise 1: F1 Hybrid Cross Results: Punnett Square
Generation Expected Alleles V Expected Alleles V P , dominant x recessive ______ _ppss_ F1 Progeny , _______ Frequency ... ð alleles , ¡ñ alleles v F1 Parent, genes:__________ (student fill in blank) 1. Expected phenotypic ratio of green to white ... 1 point) alleles eggs sperm colors 2. Mendel called plants that received different alleles for a trait from each parent ____. ( ... D. From the phenotype of the kernels on each P generation cob what would the predicted genotype of any F1 plant be?. E. Given ...https://www.jiskha.com/display.cgi?id=1303676564
Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with...
Generation of temperature-sensitive alleles of PpPex1p. The first step in identifying the cellular partners of PpPex1p was the ... generation of temperature-sensitive PpPEX1 alleles for use in a suppressor screen. The PpPEX1 ORF was subjected to a PCR-based ... Temperature-sensitive alleles of PpPEX1 were generated by a procedure described earlier (22). pJAH17 (11) is an autonomously ... Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of ...http://pubmedcentralcanada.ca/pmcc/articles/PMC108805/
Anette Preiss and Hohenheim Universität - Free people check - Yasni.com
PLOS ONE: Generation of New Hairless Alleles by Genomic Engineering.... journals.plos.org ...http://www.yasni.com/anette+preiss/check+people/hohenheim+universit%C3%A4t
Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology | Genome Biology | Full Text
... and enables the rapid generation of both simple and complex alleles. ... The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient ... a Generation of knockout and knockin alleles using embryonic stem (ES) cell technology in mice. A cloning procedure is ... For the generation of knockout alleles, the microinjection of a single guide RNA (sgRNA) in zygotes is sufficient to create ...https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1409-1
Unique and Shared Functions of Nuclear Lamina LEM Domain Proteins in Drosophila | Genetics
Generation of bocks deletion alleles. The parental line used to generate bocks mutants was bocksCB03586, a line that carries a ... Generation and analysis of bocks deletion alleles. (A) Structure of the bocks locus, including a portion of the uncharacterized ... This strategy produced two bocks deletion alleles, bocksΔ10 and bocksΔ66. Deletions in both alleles extend 5′ from +11, with ... Deletion alleles of bocks were generated by imprecise deletion of a P element inserted at +11 in the bocksCB03586 allele ( ...http://www.genetics.org/content/197/2/653
Reporter Gene Insertions Reveal a Strictly B Lymphoid-Specific Expression Pattern of Pax5 in Support of Its B Cell Identity...
Generation of two Pax5 alleles with different reporter gene insertions. A, Structure of the targeted Pax5 alleles. The ... The Pax5ihCd2 and Pax5iGFP alleles were backcrossed for seven generations or more into the C57BL/6 genetic background. The Pax5 ... Generation of two Pax5 alleles with distinct reporter gene insertions. The expression of Pax5 was so far determined in cultured ... Similar expression of both Pax5 alleles throughout B cell development. To monitor the expression of each Pax5 allele, we ...http://www.jimmunol.org/content/178/5/3031
Intraflagellar transport is essential for endochondral bone formation | Development
Generation of a conditional allele of. Ift88 As Ift88 is expressed in most cell types throughout the mammalian body, and ... Generation of a conditional allele of Ift88. (A) Schematic representation of the targeting construct and the Ift88 genomic ... Overall, the generation of a conditional allele of the IFT protein Ift88 provides a valuable resource to allow the study of IFT ... B) Wild-type, floxed and null alleles (generated from the floxed allele) of Ift88 were distinguished by PCR using genomic DNA ...http://dev.biologists.org/content/134/2/307
Generation of the Gabra5 floxed allele and the conditional KO mice. A replacement-type targeting vector (total genomic homology ... 1A, top). The floxed allele was bred to the C57BL/6J background (The Jackson Laboratory) for at least 13 generations and ... A, Generation of α5DGKO mice. P1-P3, PCR primers (loxP sites and primers not drawn to scale). B, False color image of DAB ... Generation of α5DGKO mice. The DG-selective reduction in α5-GABAAR expression (Fig. 1B-E′) was confirmed through ...http://www.jneurosci.org/content/35/40/13698
Biology-Online • View topic - Gene Frequency
Than your new allele frequencies (call them p1 and q1 for F1 generation) derived from this:. p1 = Frequency of AA + ½ Frequency ... Remember, the P generation dominant allele frequencies are 0.6 and 0.2.. (0.6 + 0.2) ÷ 2 = 0.4. (0.4 + 0.8) ÷ 2 = 0.6. So, it ... Genetic equilibrium means that the ratio of alleles is not changing between generations, but it has nothing to do with what ... In Mednel's punnet square, you give a 50%50 chance to each allele (two rows/columns; one for each allele). However, you're ...http://www.biology-online.org/biology-forum/about25997.html?p=144847&hilit=Chance
Generation of the Floxed p53 Allele.. We engineered the floxed p53 allele (designated p53fp) by inserting the loxP sites into ... Generation of conditionally inactivated p53 alleles. A, maps of a portion of the wild-type p53 locus, the targeting construct ... Generation of Conditional p53 Mutant Mice.. J1 embryonic stem cells were electroporated with SalI-linearized p53neoloxp32TK and ... A, PCR analysis of Cre-mediated excision of floxed p53 alleles in p53fp/fp; Cre mice. Primer pair x/z amplified a ∼500-bp ...http://cancerres.aacrjournals.org/content/64/10/3525
no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila | Development
Generation of the nopo-null allele. A nopo-null allele was generated by imprecise excision of P-element EYG5845. The 771-bp ... We generated a null allele of nopo (Exc142) via imprecise excision of EYG5845 (Fig. 2A; see Materials and methods). nopoExc142 ... A conserved residue in the RING domain is altered in our EMS-mutagenized allele of nopo, suggesting that E3 ligase activity is ... To gain further insights into the functions of nopo, we obtained additional alleles. 5-SZ-3004 (abbreviated as SZ3004) and ...http://dev.biologists.org/content/136/3/449
Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules | JCB
Generation of the klar null allele. To generate the klar null allele, FLP-mediated recombination was used between two PBac ... We used the alleles klarΔ1-18, which deletes the entire klar locus, and msp-300Δ3′, which deletes roughly half of the 3′ region ... Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Nat. Genet. 36:288-292. doi: ... S1 shows a scheme of MSP-300 and klar gene loci and the various alleles used in the present study. Fig. S2 shows confocal scans ...http://jcb.rupress.org/content/198/5/833.long
Dual Roles of Glutathione in Ecdysone Biosynthesis and Antioxidant Function During Larval Development in Drosophila | Genetics
Generation of Gclc loss-of-function mutant allele. Generation of the Gclc allele was carried out by the clustered regularly ... Generation of complete Gclc loss-of-function allele in D. melanogaster. We first generated a complete Gclc loss-of-function ... After these procedures, we eventually selected the Gclc46 allele. Since this allele has a large (46 bp) deletion in the coding ... In the next generation, the number of adult offspring males (Gclc46/Y with GAL4 and UAS) and females (Gclc46/+ with GAL4 and ...http://www.genetics.org/content/207/4/1519