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DNA microarrays can be used to measure genome-wide transcript levels. These measurements may be useful in understanding cellular changes induced by a chemical agent. In this study, Affymetrix microarray technology has been used to study the effects of chloramphenicol, an antibiotic that inhibits bacterial and mitochondrial protein synthesis, on the transcription profile in mammalian cells. Transcript levels in BB88 murine erythroleukemia cells treated with 50 micromolar ( mu M) chloramphenicol, a concentration shown to inhibit BB88 proliferation, are measured. Using total RNA from treated cells, biotin-labeled cRNA was prepared and hybridized to Affymetrix U74Av2 microarrays. From these hybridizations, expression levels were measured for approximately 12,000 transcripts. Methods to identify genes whose transcript levels increase or decrease during the treatments are described. In particular, chloramphenicol increases transcript levels for a ...
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The chloramphenicol resistant pUC vectors pHSG396 and pHSG398 contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These chloramphenicol resistant pUC vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter on both the pHSG396 and pHSG398 vectors. ...
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Chloramphenicol is a generic name antibiotic that can eradicate or stall bacterial infections.. How It Works Chloramphenicol is a broad spectrum activity antibiotic used to treat bacterial infections in various parts of the body, including the eye. This medicine is metabolized in the liver and can penetrate bacterial cells to stop protein synthesis. When the cells can no longer form peptide bonds, the bacteria eventually die and the infection clears up. Chloramphenicol is only prescribed when other types of antibiotics are deemed ineffective against the infection.. Uses. Chloramphenicol was originally intended to treat typhoid. However, as the Salmonella type bacteria that causes the disease has become drug resilient, Chloramphenicol is only recommended when the microorganism is known to be vulnerable to the drug. Chloramphenicol is an effective treatment for cholera, ...
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If you go back a few years there was the case of the "honey laundering" where honey from China was first sent to Australia and then exported to the US basically at the time to avoid trade barriers. With transparency we certainly are finding out what is in the food that comes from China and we can all remember back to the melamine incident with mile and dried milk products. The FDA has banned chloramphenicol for use in any food products and even big companies like Sue Bee are importing honey from China and they have their own internal testing for the drug and are still finding it in their samples in their lab. When Sue Bee returns the product there's no word on how it may or may not get back into the pipeline by potentially being sold to someone else. Argentina, Vietnam and Canada are also big exported of honey to the US as bee colonies in the US continue to dwindle. BD. ...
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The Pfizer team recruited a Nigerian doctor to act as its leader, who has since claimed that he was little more than a front man. They then administered Trovan in oral form to 100 children selected from meningitis sufferers admitted to a Kano field hospital.. The Pfizer team also administered cef-triaxone, a registered drug for meningitis, in lower-than-recommended doses to another 100 children also suffering from the illness.. Pfizer said that it had received the verbal approval of the families of the patients, although in filing a lawsuit, the families have claimed that the company did not obtain their consent.. Meanwhile, those children admitted to the hospital who were not selected by the Pfizer team were treated with the correct dosages of the antibiotic chloramphenicol, an internationally accepted treatment for bacterial meningitis, by a team from Medecins Sans Frontieres.. Once the Pfizer team had ended its mission, it returned to the US, though the epidemic continued.. Of the 200 ...
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