NFAT Regulates Insulin Gene Promoter Activity in Response to Synergistic Pathways Induced by Glucose and Glucagon-Like Peptide...
... chloramphenicol acyltransferase; CRE, cAMP response element; GLP, glucagon-like peptide; NFAT, nuclear factor of activated T- ... The synergistic actions of glucose (Glc) and GLP-1 on insulin gene promoter activity are PKA-dependent. Promoter activity of ... on glucose-induced insulin promoter activity, and had no significant effect on the induction of insulin promoter activity by ... NFAT Regulates Insulin Gene Promoter Activity in Response to Synergistic Pathways Induced by Glucose and Glucagon-Like Peptide- ...http://diabetes.diabetesjournals.org/content/51/3/691
C/EBPα Is a Regulator of the UDP GlucuronosyltransferaseUGT2B1 Gene | Molecular Pharmacology
C/EPBα binds to region C of the UGT2B1 gene promoter.. As demonstrated above, the promoter activity of the −122/+14 construct ... Varying lengths of the UGT2B1 gene promoter, with and without the C/EBP-like element, were fused to the chloramphenicol ... the UGT2B1 gene promoter. The importance of C/EBPα in the regulation of the homologous mouseUGT2B1 gene was also assessed in ... we have begun to analyze the rat UGT2B1 gene promoter. The promoter is able to drive expression of the CAT and placental ...http://molpharm.aspetjournals.org/content/53/6/1027
GUS reporter system - Wikipedia
The purpose of this technique is to analyze the activity of a promoter (in terms of expression of a gene under that promoter) ... Other competing systems are based on e.g. luciferase, GFP, beta-galactosidase, chloramphenicol acetyltransferase (CAT), ... The GUS system is not the only available gene reporter system for the analysis of promoter activity. ... The GUS assay, as well as other reporter gene systems, can be used for other kinds of studies other than the classical promoter ...https://en.wikipedia.org/wiki/GUS_reporter_system
A common functional polymorphism in the promoter region of the microsomal triglyceride transfer protein gene influences plasma...
Expression studies with minimal promoter constructs linked to the chloramphenicol acetyltransferase reporter and transfected ... into HepG2 cells revealed marked enhancement of transcriptional activity with the T variant. The prevalence of the MTP promoter ... By initial opportunistic screening for polymorphic sites in the regulatory region of the MTP gene by gene sequencing in 20 ... This common genetic variation of the MTP promoter is likely to have important implications for cardiovascular disease. ...https://www.rdm.ox.ac.uk/publications/11275
US Patent # 5,004,687. Insect virus vector with broadened host range - Patents.com
Exogenous genes under the control of a promoter which promotes expression in 'non-permissive ... A novel viral expression vector is employed in the expression of exogenous genes in 'non-permissive' hosts. ... supra,) and CAT activities (Gorman, et al.,supra). Fifty or one hundred nCi (1 or 2 nmol) of .sup.14 C-chloramphenicol was used ... coli B-galactosidase gene, the lac Y gene, part of the lac A gene, a portion of the B-lactamase gene, a col E1 origin for ...http://patents.com/us-5004687.html
Structure and expression of the human slow twitch skeletal muscle troponin I gene. - PubMed - NCBI
A muscle-specific enhancer regulated slow troponin I promoter activity.. PMID:. 8144655. ... flanking DNA linked to the bacterial chloramphenicol acetyl-transferase gene, exhibited cell type-specific and developmentally ... In contrast to many muscle-specific genes, the troponin I promoter does not contain consensus CCAAT or TATA elements. Moreover ... Structure and expression of the human slow twitch skeletal muscle troponin I gene.. Corin SJ1, Juhasz O, Zhu L, Conley P, Kedes ...https://www.ncbi.nlm.nih.gov/pubmed/8144655
Evidence for transcriptional control of human mdr1 gene expression by verapamil in multidrug-resistant leukemic cells. |...
... promoter activity observed in CEM VLB100 cells transiently transfected with the mdr1 promoter fused to the chloramphenicol ... acetyltransferase gene. Indeed, after exposure to 30 microM VRP, chloramphenicol acetyltransferase activity was decreased by 2- ... Evidence for transcriptional control of human mdr1 gene expression by verapamil in multidrug-resistant leukemic cells.. C ... Evidence for transcriptional control of human mdr1 gene expression by verapamil in multidrug-resistant leukemic cells.. C ...http://molpharm.aspetjournals.org/content/47/1/51
US Patent # 5,587,361. Oligonucleotides having phosphorothioate linkages of high chiral purity - Patents.com
This vector contains the bacterial chloramphenicol acetyl transferase gene under regulatory controls of the SV40 promoter. ... The activity of a phosphorothioate having substantially chirally pure intersugar linkages in inhibition of gene expression may ... PAP has advantages over CAT as a reporter gene in that it is a mammalian gene, rather than a bacterial gene that contains ... Marcus-Sekura, C. J. et al., "Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense ...http://patents.com/us-5587361.html
Downregulation of Renin Gene Expression by Interleukin-1 | Hypertension
CAT activities were given as percent conversion of chloramphenicol obtained in the enzyme assay with 35 μg of cellular protein ... Role of proximal promoter elements in regulation of renin gene transcription. J Biol Chem. 1996;271:22499-22505. ... Equal amounts of protein (35 μg) were assayed for CAT activity in 0.25 mol/L Tris, pH 8.0, 0.125 μCi of [14C]-chloramphenicol ( ... In an effort to control for this, an RSV promoter driving CAT reporter gene (RSV-CAT) was used to determine nonspecific effects ...http://hyper.ahajournals.org/content/30/2/230
KAKEN - Research Projects | Regulation of the plant defense gene expression (KAKENHI-PROJECT-03044100)
Chimeric DNA containing PAL promoter fragment connected to luciferase gene (lux), beta-glucuronidase (GUS) or chloramphenicol ... however those with PAL promoter did not exhibit GUS or Lux activities even by the treatment with fungal elicitor. Furthermore, ... Cytological studies showed that plants with Lux or GUS gene connected to 35S promoter as a control exhibited the distinctive ... 2. Analysis of CHS gene. The structure and the organization of three major CHS genes were determined from 38 CHS-cDNAs prepared ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03044100/
Calculations for Molecular Biology and Biotechnology - 2nd Edition
11.9 Using β-Galactosidase to Monitor Promoter Activity and Gene Expression. 11.9.1 Assaying β-Galactosidase in Cell Culture. ... 11.12 The Chloramphenicol Acetyltransferase (CAT) Assay. 11.12.1 Calculating Molecules of Chloramphenicol Acetyltransferase ( ... 9.5 Measuring Gene Expression. 9.6 Relative Quantification - The ΔΔCT Method. 9.6.1 The 2-ΔΔCT Method - Deciding on an ... 11.9.2 Specific Activity. 11.9.3 Assaying β-Galactosidase from Purified Cell Extracts. 11.10 Thin Layer Chromatography (TLC) ...https://www.elsevier.com/books/calculations-for-molecular-biology-and-biotechnology/stephenson/978-0-12-375690-9
CBFα3 (AML2) Is Induced by TGF-β1 to Bind and Activate the Mouse Germline Ig α Promoter | The Journal of Immunology
... we examined the effects of mutations in the TβRE on reporter gene activity. The GL α promoter −130/+14 or −132/+14 segments ... Transfection, luciferase, chloramphenicol acetyltransferase (CAT), and β-galactosidase (β-gal) assays. For each transfection, ... but also reduces basal promoter activity by about 50% (Fig. 6⇑). Both mutations reduce the transcriptional activity of the ... Overexpression of CBFα proteins in A20.3 cells increased the GL α promoter activity by 3- to 7-fold in the absence of TGF-β1 ( ...http://www.jimmunol.org/content/161/12/6751
Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells Y. Higashimoto,1 N. ... The bicistronic constructs containing the firefly luciferase gene as the first cistron and the chloramphenicol ... GENE EXPRESSION ABSTRACTS VOLUME 8, NUMBER 5-6. Gene Expression, Vol. 8, pp. 263-272, 1999 1052-2166/99 $20.00 + .00 Copyright ... After stimulation of A549 cells with TNF-a, IFN-g, or LPS, promoter activity was increased by two- to threefold in the presence ...https://www.cognizantcommunication.com/cccSiteFiles/Gene/geneabs8-5.htm
Caracterização da região promotora do cístron de RNA ribossômico em duas linhagens...
... com base em sequências de genes de rDNA e mini-exon e análise por RAPD (Souto et al. 1996). Neste trabalho investigamos... ... The activity of L1 and L2 promoters was investigated through the use of plasmid constructs bearing bacterial chloramphenicol ... Duas linhagens filogenéticas principais (LI e L2) foram definidas em Trypanosoma cruzi, com base em sequências de genes de rDNA ... It was observed that both promoters were functional in this group of strains, although L2 promoter drove higher CAT activity. ...http://www.teses.usp.br/teses/disponiveis/46/46131/tde-05082008-142932/pt-br.php
Functional Impact of Collagens on the Activity Directed by the Promoter of the α5 Integrin Subunit Gene in Corneal Epithelial...
Methods: Responsiveness of the α5 gene toward collagen was determined by transfection of α5 promoter/chloramphenicol ... α5 gene promoter toward FN.36 In RCECs grown on CIV (that causes a more severe repression of α5 promoter activity), both the ... Functional Impact of Collagens on the Activity Directed by the Promoter of the α5 Integrin Subunit Gene in Corneal Epithelial ... 1D). We therefore conclude that collagens negatively regulate the activity of the α5 gene promoter in RCECs grown to confluence ...https://iovs.arvojournals.org/article.aspx?articleid=2451413
Modified SAK gene for the production of recombinant proteins - Lupin Limited
The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which impart ... The present invention relates to modified SAK gene having amino acid SEQ ID 2. ... Expression of the gene of interest is achieved in shake flask studies. Briefly, 50 ml of LB with amp and chloramphenicol was ... Genes of interest are cloned in pET plasmids under control of strong bacteriophage T7 promoter carrying transcription and ...http://www.freepatentsonline.com/8853380.html
A, NO modulates ET-1-increased β-MHC promoter activity. Cardiomyocytes were transfected with 15 μg of β-MHCCAT chimeric gene. ... The relative chloramphenicol acetyltransferase activity was corrected by normalizing the respective CAT value to that of β- ... fos promoter activity nor PTIO-enhanced, ET-1-stimulated c-fos promoter activity (Fig. 4B). Cardiomyocytes cotransfected with ... fos promoter activity. The addition of SNAP to cardiomyocytes significantly inhibited the increased c-fos promoter activity by ...http://molpharm.aspetjournals.org/content/68/4/1183
Recombinant bacteria for mosquito control | Journal of Experimental Biology
Amp, ampicillin-resistant gene; Erm, erythromycin-resistant gene; Cm, chloramphenicol-resistant gene; cyt1A-p, cyt1A promoters ... Wirth, M. C., Walton, W. E. and Federici, B. A. (2000b). Cyt1A from Bacillus thuringiensis synergizes activity of Bacillus ... The second plasmid (pPFT11Bs-CRP) contained the cry11B gene under the control of cyt1A promoters and the STAB/SD sequence. The ... along with the cyt1A gene and the 20-kDa chaperone-like protein, under the control of the cry1Ac promoters (Wu and Federici, ...http://jeb.biologists.org/content/206/21/3877
ORBi: Browsing ORBi
... little activity was detected with the promoter of the DNA polymerase gene (ORF28). No activation of late gene expression, ... promoter/regulatory regions from genes belonging to the three putative VZV kinetic classes fused to the chloramphenicol ... IE62 and IE63 can regulate the expression of an indicator gene driven by various VZV promoter regions, demonstrating that these ... When various amounts of ORF4 were transfected in the presence of early gene promoters, dose-dependent transactivation was ...http://orbi.ulg.ac.be/browse?type=author&value=Melen-Lamalle,%20Laurence%20p002363
Cloning and analysis of the promoter region of the rat SM22α gene | Biochemical Journal
The residual promoter activity in the smallest fragment of the SM22 alpha promoter tested suggested that, unlike the smooth- ... A 1.5 kb fragment at the 5′ end of this sequence was able to drive the expression of a reporter chloramphenicol ... Cloning and analysis of the promoter region of the rat SM22α gene. P R Kemp, J K Osbourn, D J Grainger, J C Metcalfe ... Cloning and analysis of the promoter region of the rat SM22α gene ...http://www.biochemj.org/content/310/3/1037
Patente WO1999052357A1 - Assays for inhibitors of bacterial translation initiation factor 3 - Google Patentes
The figure is a schematic diagram of the pAUU-CAT reporter gene construct in which the CAT gene has its ATG initiator codon ... The invention also encompasses reporter gene constructs, test cells, and compounds identified by the methods of the invention. ... to result in increased growth of specially-designed test cells due to higher levels of expression from the reporter gene. The ... The assay uses a reporter gene system in whole cells, and is based on the ability of IF3 to discriminate against translation ...http://www.google.es/patents/WO1999052357A1?cl=en
Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A laboratory model of molecular...
ampR activity of various regions of the DNA fragment. The regions shown here were put downstream of the lacZ promoter of ... The exact activity of this gene fragment also remains unclear: so far, neither β-lactamase, kinase, nucleotidyltransferase, nor ... about 100 largest colonies were picked up and spotted on an LB agar plate containing 30 μg/ml chloramphenicol, and the plate ... The 5′ region of the shuffled fragment would contain a promoter sequence, because this plasmid shows significant ampR activity ...http://www.pnas.org/content/98/3/903
Activation of the RON receptor tyrosine kinase inhibits induci...
Further, we show that activated RON inhibited the iNOS gene transcription activity as assessed by chloramphenicol ... prevented the inhibitory effect of RON on the iNOS gene promoter activity and on the nitric oxide production induced by LPS and ... acetyltransferase activity in Raw264.7 cells expressing RON. Wortmannin, a specific inhibitor of phosphatidylinositol-3 (PI-3) ...https://www.mysciencework.com/publication/show/activation-ron-receptor-tyrosine-kinase-inhibits-inducible-nitric-oxide-synthase-inos-expression-murine-peritoneal-exud-2286c77b
Patente US8093043 - β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing ... - Google Patentes
... or RSK2 reporter gene promoter activity system. Reporter genes for use in the invention encode detectable proteins, and include ... but are by no means limited to, chloramphenicol transferase (CAT), β-galactosidase (O-gal), luciferase, green fluorescent ... Gene or Structural Gene. The term "gene", also called a "structural gene" means a DNA sequence that codes for or corresponds to ... the trp-lac promoter, the bacteriophage lambda PL promoter, the T7 promoter, the beta-lactamase promoter (Villa-Komaroff, et al ...http://www.google.es/patents/US8093043?hl=es&dq=flatulence
IJMS | Free Full-Text | Tailoring the Models of Transcription | HTML
The understanding of promoter complexity and transcription factor structure offers an insight into the mechanisms of ... This review focuses on some of the many applications of molecular cut-and-paste tools for the manipulation of promoters and ... which have become versatile tools for manipulating promoters, as well as transcription factors in order to examine their ... The study of transcriptional regulation has been greatly facilitated by the development of reporter genes and transcription ...http://mdpi.com/1422-0067/14/4/7583/htm