... chloramphenicol acyltransferase; CRE, cAMP response element; GLP, glucagon-like peptide; NFAT, nuclear factor of activated T- ... The synergistic actions of glucose (Glc) and GLP-1 on insulin gene promoter activity are PKA-dependent. Promoter activity of ... The synergistic actions of glucose (Glc) and GLP-1 on insulin gene promoter activity are PKA-dependent. Promoter activity of ... on glucose-induced insulin promoter activity, and had no significant effect on the induction of insulin promoter activity by ...
In this way, strong promoter activity produces much staining and weak promoter activity produces less staining. The uidA gene ... Other competing systems are based on e.g. luciferase, GFP, beta-galactosidase, chloramphenicol acetyltransferase (CAT), ... The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so- ... The GUS system is not the only available gene reporter system for the analysis of promoter activity. ...
The chloramphenicol acetyl transferase gene (CAT) present between t0 and T1 has no promoter and is not normally expressed. ... Depending on the bacterial strain and insert,low CAT activities may be detectable ... 1. Optimized promoter/operator element. Consists of the phage T5 promoter and two lac operator sequences, ... There is the T5 promoter/lac operator transcription-translation system for expression inE. coli, the p10 promoter for ...
Tissue-specific activity of the pro-opiomelanocortin gene promoter., Jacques Drouin, Chamberland M, Plante RK, Trifiro MA, ... Chick embryo cells rendered respiration-deficient by chloramphenicol and ethidium bromide are auxotrophic for pyrimidines., ... Hoxa5 gene regulation: A gradient of binding activity to a brachial spinal cord element., Tuggle CK, Jeannotte L, Nguyen-Huu MC ... Sustained Hox5 gene activity is required for respiratory motor neuron development., Dasen JS, Jeannotte L, Aubin J, Walsh CM, ...
... a chloramphenicol acetyltransferase gene linked to an estrogen response element upstream of thymidine kinase promoter was ... and the amount of chloramphenicol acetyltransferase activity, an indicator of estrogen responsiveness, was found to increase 20 ... which were further analyzed by gene ontology classification. A subset of highly regulated genes was validated using ... UTERINE SMOOTH MUSCLE CELLS EXHIBIT AGONIST-LINKED NITRIC OXIDE SYNTHASE ACTIVITY 3rd International Meeting on the Biology of ...
... gene was analysed by transient transfection assays employing chloramphenicol acetyl transferase (CAT) reporter constructs. We ... Promoter activity of sequence located upstream of the pseudorabies virus early protein 0 gene. In: Veterinary Microbiology. ... N2 - Promoter activity of the 5-flanking region of the pseudorabies virus (PRV) early protein 0 (EP0) gene was analysed by ... AB - Promoter activity of the 5-flanking region of the pseudorabies virus (PRV) early protein 0 (EP0) gene was analysed by ...
Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue ... gene expression regulation,gene library,glycoproteins,humans,introns,mice,molecular sequence data,point mutation,promoter ... Transcriptional activity of the human tissue inhibitor of metalloproteinases 1 (TIMP-1) gene in fibroblasts involves elements ... gene in fibroblasts involves elements in the promoter, exon 1 and intron 1. Biochemical Journal, 324 (2). pp. 611-617. ISSN ...
... activity in cells transfected with pRNH11c containing an Ah-responsive human CYP1A1 gene promoter insert (-1142 to +2434). ... activity and a 10.5-fold increase in chloramphenicol acetyltransferase (CAT) ... In contrast, neither estrogen nor progesterone inhibited EROD activity induced by TCDD in Ishikawa cells, whereas inhibitory ER ... transfected with E2-responsive constructs containing promoter inserts from the progesterone receptor and vitellogenin A2 genes ...
The rat glycoprotein hormone LHβ-subunit promoter, linked to the chloramphenicol acetyl transferase (CAT) reporter gene, was ... The rat glycoprotein hormone LHβ-subunit promoter, linked to the chloramphenicol acetyl transferase (CAT) reporter gene, was ... The rat glycoprotein hormone LHβ-subunit promoter, linked to the chloramphenicol acetyl transferase (CAT) reporter gene, was ... The rat glycoprotein hormone LHβ-subunit promoter, linked to the chloramphenicol acetyl transferase (CAT) reporter gene, was ...
... whereas gene product levels of the chloramphenicol acetyltransferase (CAT) or β-galactosidase (βGal) gene were not. Neither ... We found that the target of heavy metals is the reporter, rather than the promoter used in the reference plasmid. Of the ... Agriculture was the most common economic activity (total 48 cases), with cattleman and dairyman (total 26 cases) the most ... The transfection assay is an important tool for evaluating the health effects of industrial chemicals, with the reporter gene ...
... indicated that cAMP primarily stimulated hGH promoter activity. Cotransfection of a protein kinase A inhibitory protein cDNA ... Analysis of the intact hGH gene or hGH 5- flanking DNA (5-FR) coupled to the hGH cDNA or chloramphenicol acetyltransferase or ... Analysis of the intact hGH gene or hGH 5- flanking DNA (5-FR) coupled to the hGH cDNA or chloramphenicol acetyltransferase or ... luciferase genes, indicated that cAMP primarily stimulated hGH promoter activity. Cotransfection of a protein kinase A ...
... conferred a similar cold shock response to the expression of chloramphenicol acetyltransferase (CAT) activity in vivo and in ... recognizes some feature of the 110-bp promoter region of hns and acts as a cold shock transcriptional activator of this gene ... recognizes some feature of the 110-bp promoter region of hns and acts as a cold shock transcriptional activator of this gene ... DNA fragment containing the promoter of hns fused to a promoterless cat gene (hns-cat fusion) conferred a similar cold shock ...
In vivo and in vitro biological activities of the ,b ,chn,/b, complex were compared with the chemical nuclease [Cu(phen)(nal), ... as a substrate a 196 bp PCR fragment encompassing part of the open reading frame and the promoter regions of the ytkD gene from ... Chloramphenicol was added to the culture medium at a final concentration of 3 𝜇. g/mL. ... Biological Activity Studies of [Cu(hsm)(nal. H. 2. O. C. l. ⋅. 3. H. 2. O. Comparing with [Cu(phen)(nal. H. 2. O. N. O. 3. ⋅. 3 ...
... of replication in pQE thirty is ColE1 and transcription of your inserted gene is managed by the bacteriophage T5 promoter and ... The highest certain activity was determined for being 15. five Umg. Michaelis Menten style kinetics could only be observed ... The resulting chloramphenicol resistant strain JM109 pRARE2 was the expression host. Cloning of choA from C. gleum The putative ... Evaluated the mRNA gene expression profile of 12 HDAC genes by quantitative serious time polymerase chain reaction in 94 ...
Use to confirm the function of the products of genes or cDNAs that are thought to encode a phytoene synthase enzyme, or to ... A yellow colony color and an accumulation of beta-carotene is indicative of phytoene synthase activity. ... For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. ... Promoter endogenous promoters Cloning Information * Cloning method Restriction Enzyme * 5′ cloning site EcoRI (not destroyed) ...
... reduce expression of an endogenous gene for 3- phosphoadenosine-5-phosphosulfate reductase (cysH); and express one or more ... coli host cells were modified so as to reduce expression of an endogenous gene for fructosyltransferase (kfoE); ... 229960005091 Chloramphenicol Drugs 0.000 description 1 * WIIZWVCIJKGZOK-RKDXNWHRSA-N Chloramphenicol Chemical compound ClC(Cl)C ... 0036] T7 RNA polymerase gene with lacUV5 promoter was integrated into the LacZ position in the E. coli K4 genome. Briefly, a ...
A promoter analysis was performed using transient transfection of chloramphenicol acetyl transferase reporter gene constructs ... These results suggest the genes for CB receptors are absent in C. elegans, and the nematicidal activities of δ‐THC and Cannabis ... We also found an anandamide amidase activity in the leech CNS cytosolic fraction with a maximal activity at pH 7 and little ... Several C. elegans gene products with low homology to CB receptors and FAAH were identified. Close examination of these ...
Plasmid pRM100 is a pWSK29 (44) derivative that contains the complete polA + gene (coding sequence and promoter) and was ... E. coli Pol I contains three distinct activities: (i) a 5′-to-3′ template-directed DNA polymerase activity, (ii) a 3′-to-5′ ... When necessary, the following antibiotics were added at the indicated concentrations: ampicillin, 150 mg/ml; chloramphenicol, ... Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. J. Bacteriol. 158 : 636- ...
rpoS promoter region/nlpD gene (Stoebel et al., 2009), potD (Shah et al., 2011), soxSR (Jain and Saini, 2016) (Supplementary ... Chloramphenicol. CHL. Chloramphenicol. Protein synthesis, 50S. Bacteriostatic. 2. Erythromycin. ERY. Macrolides. Protein ... Decreased intracellular level of the probe indicates either decreased porin activity or enhanced efflux-pump activity (Coldham ... Nevertheless, we noted several examples on functional relatedness between resistance genes mutated in T0 and genes mutated ...
... influences the overall gene expression in S. aureus. Deleted and complemented S. aureus strains were analyzed for their ... Regulatory Landscape of the Root-Knot Nematode Meloidogyne incognita and a Promoter Motif Associated with Effector Genes ... PPIases are required for the correct folding and subsequent activity of secreted virulence factors in a number of bacterial ... supplemented with chloramphenicol (10 µg/mL) at 37 °C with vigorous shaking (200 rpm) (optimal conditions). For stress ...
In a promoter-specific context, inhibition of H3R17 methylation represses expression of p21, a p53-responsive gene, thus ... Specific sites of histone tail methylation are associated with transcriptional activity at gene loci. These methyl marks are ... reduced levels of Tat transactivation of HIV-1 long terminal repeat chloramphenicol acetyltransferase and luciferase reporter ... Thus, SET prevents promoter acetylation and is a prerequisite for the initial acetylation-sensitive steps of pS2 gene ...
... gene encoding the protein targeted by antifungal azoles) with or without a tandem repeat (TR) insertion in its promoter (6). ... We then plated 100 μL of these suspensions on Sabouraud dextrose agar supplemented with chloramphenicol (50 mg/L; Sigma-Aldrich ... some of which have been shown to have molecular targets identical to those of medical triazoles and have activity against A. ... Sequencing of the cyp51A gene and its promoter showed that 19 ARAF isolates harbored 3 commonly identified nucleotide and amino ...
Reduced activity in vitro [44]. 5UTR c.-66T > C rs2252281 0.445 68 African Americans Decreased promoter activity. in vitro and ... The proximal promoter of the human SLC47A1 gene does not. contain a canonical TATA or CCAAT box for basal transcrip￾tion but ... cephradine, chloramphenicol, chloroquine, ciprofloxacin,. clotrimazole, ketoconazole, levofloxacin, pentamidine,. proguanil, ... common promoter variant rs2252281 (c.-66T > C) reduces. promoter activity resulting in a lower MATE1 mRNA expres￾sion in human ...
Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors - Artículo de Revista ... A candidate gene approach identifies an IL33 genetic variant as a novel genetic risk factor for GCA - Artículo de Revista ... Physical activity domains and risk of gastric adenocarcinoma in the MCC-Spain case-control study - Artículo de Revista ... Gene expression profiling in human neutrophils after infection with Acinetobacter baumannii in vitro - Artículo de Revista ...
The chloramphenicol acetyltransferase cat gene from plasmid pGK12 15 was amplified by rainbow six siege skin changer download ... Nanda informed the Board injectors in order to consolidate hack expand the activities of the company, an Executive Director be ... and transcriptionally fused to the strong P32 promoter amplified from plasmid pMG36e. Our fortune on the sea is out of breath, ... Measurement of the serum leptin level could assist disease activity monitoring in rheumatoid arthritis. Creating computer-based ...
Extensive Characterisation of new B.subtilis biobricks, Chloramphenicol resistance gene and motility ... Our activities are summarized on this page. To find out more please visit the Dry Lab Hub. ... Characterized the new promoter and ribosome binding sites biobricks BBa K143079 and BBa K143082 that we submitted this year. ... AB is our antibiotic resistance cassette, ytvA is the gene controlling the light-sensing pathway, SB is the biomaterial, epsE ...
The intrinsic limitation of PBAD for the gene expression is known to be negated by glucose and afflicted with all-or-nothing ... EcN was armed with HlyE, a small cytotoxic protein, under the control of the araBAD promoter (PBAD). ... To approach the goal, HlyE was expressed under the control of the araBAD promoter (PBAD). The strategy of metabolic engineering ... The antitumor activity of EcNe/HlyE was investigated in tumor-bearing mice. The mice xenografted with either SW620 or HT29 ...
Polymorphisms of the angiotensinogen gene, the endothelial nitric oxide synthase gene, and the interleukin-1beta gene promoter ... At least in the case of the IL1B promoter, repression did not seem to involve another factor whose activity is affected by the ... In our transient transfection assays using human THP-1 monocytic cells, a chloramphenicol acetyltransferase (CAT) construct ... Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with ...
Furthermore, transfection of RAW 264.7 cells with iNOS promoter linked to a chloramphenicol acetyltransferase reporter gene, ... Caffeic acid phenethyl ester inhibits nitric oxide synthase gene expression and enzyme activity.. Song YS, Park EH, Hur GM, Ryu ... In conclusion, CAPE was shown to possess antioxidant activity by saving SOD activity, preventing XO activity and decreasing the ... its antiradical activity and plant origin.. Christov R, Trusheva B, Popova M, Bankova V, Bertrand M.. Regional. Center. for ...
Key Words: Promoter activity, Gene regulation, E. coli, ClpYQ (HslUV), ATP-dependent protease, 5 stem-loop structure, mRNA ... and chloramphenicol (12.5 μg/ml) with an addition of arabinose (0.5 %) to induce the PBAD promoter. The overnight cultures were ... lacZ fusion genes were made; in each one, the clpQ+Y+ promoter is in front of lacZ. Both fused genes have the lacZ gene under ... ClpQ+Y+ Promoter-Mediated Basal and Heat Shock-Induced Gene Expression Requires RpoH. It was shown that clpQ+Y+ promoter has ...