UCN-01 suppresses thymidylate synthase gene expression and enhances 5-fluorouracil-induced apoptosis in a sequence-dependent ... Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene. Genes Dev 2001;15:2203-8. ... Lack of PTEN expression in non-small cell lung cancer could be related to promoter methylation. Clin Cancer Res 2002;8:1178-84. ... Nonetheless, gene therapy with wild-type PTEN has been done in vivo in a mouse model of lung cancer in which aerosolized PTEN ...http://clincancerres.aacrjournals.org/content/12/3/679
Determinants of prognosis and response to therapy in colorectal cancer | SpringerLink
Johnston PG, Lenz H-J, Leichman CG, et al.: Thymidylate synthase protein and gene expression predicts for response to 5- ... Low gene expression levels of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), and thymidine phosphorylase (TP ... Lenz H-J, Leichman CG, Danenberg KD, et al.: Thymidylate synthase expression in adenocarcinoma of the stomach: a predictor for ... Lenz H-J, Danenberg KD, Leichman CG, et al.: P53 and thymidylate synthase expression in untreated stage II colon cancer: ...https://link.springer.com/article/10.1007%2Fs11912-001-0008-5
A Novel Single Nucleotide Polymorphism within the 5′ Tandem Repeat Polymorphism of the Thymidylate Synthase Gene Abolishes USF...
Thymidylate synthase (TS) gene expression is modulated by a polymorphism in the 5′ regulatory region of the gene. The ... of a polymorphic repeat sequence in the thymidylate synthase gene affect translational efficiency but not its gene expression. ... Polymorphic tandem repeats in the thymidylate synthase gene is associated with its protein expression in human gastrointestinal ... Human thymidylate synthase gene: isolation of phage clones which cover a functionally active gene and structural analysis of ...http://cancerres.aacrjournals.org/content/63/11/2898
Thymidylate Synthase as a Predictor of Response | Cancer Network | The Oncology Journal
... gene expression might be used to select therapy for patients with disseminated colorectal cancer. We recently 1 ... It has been hypothesized that intratumoral thymidylate synthase (TS) ... intratumoral thymidylate synthase (TS) gene expression might be used. to select therapy for patients with disseminated ... thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene. expression in human tumors using the polymerase chain ...http://www.cancernetwork.com/review-article/thymidylate-synthase-predictor-response
Expression of uracil DNA glycosylase does not affect cellular sensitivity to thymidylate synthase (TS) inhibition. Eur J Cancer ... Colorectal tumours responding to 5-fluorouracil have low gene expression levels of dihydropyrimidine dehydrogenase, thymidylate ... thymidylate synthase. Introduction. 5-Fluorouracil (FU) is widely used in the treatment of a range of cancers, particularly ... Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim Biophys Acta 2002; 1587: 194-205. ...http://cancerres.aacrjournals.org/content/67/3/940
Combination of Thymidine Phosphorylase Gene Transfer and Deoxyinosine Treatment Greatly Enhances 5-Fluorouracil Antitumor...
Colorectal tumors responding to 5-fluorouracil have low gene expression levels of dihydropyrimidine dehydrogenase, thymidylate ... indicative of thymidylate synthase as the main drug target. The impact of this double modulation was next investigated in ... Prognostic role of thymidylate synthase, thymidine phosphorylase/platelet-derived endothelial cell growth factor, and ... thymidylate synthase; UP, uridine phosphorylase; wt, wild-type; HPLC, high-performance liquid chromatography. ...http://mct.aacrjournals.org/content/1/2/133
Thymidylate Synthase and Methylenetetrahydrofolate Reductase Gene Polymorphisms and Toxicity to Capecitabine in Advanced...
Bathe OF, Franceschi D, Livingstone AS, Moffat FL, Tian E, Ardalan B. Increased thymidylate synthase gene expression in liver ... Thymidylate synthase gene polymorphism determines response and toxicity of 5-FU chemotherapy. Pharmacogenomics J 2001;1:65-70. ... A common insertion/deletion polymorphism of the thymidylate synthase (TYMS) gene is a determinant of red blood cell folate and ... Thymidylate synthase gene polymorphism predicts toxicity in colorectal cancer patients receiving 5-fluorouracil-based ...http://clincancerres.aacrjournals.org/content/14/3/817
Thymidylate synthase - Wikipedia
2002). "Thymidylate synthase as a translational regulator of cellular gene expression". Biochim. Biophys. Acta. 1587 (2-3): 174 ... Pyrimidine analogues Thymidylate synthase inhibitor Thymidine kinase Thymidine kinase in clinical chemistry Thymidylate kinase ... "Structural and functional analysis of the human thymidylate synthase gene". J. Biol. Chem. 265 (33): 20277-20284. PMID 2243092 ... Thymidylate synthase is an enzyme of about 30 to 35 kDa in most species except in protozoan and plants where it exists as a ...https://en.wikipedia.org/wiki/Thymidylate_synthase
Protocols and Video Articles Authored by Gustavo B. Baretton
To investigate the predictive value of Ki67 and p53 and their correlation with thymidylate synthase (TS) gene expression in a ... Posttherapeutical TS-gene, TP-gene, and DPD-gene expression on surgical resection specimens was quantified by TaqMan real-time ... Furthermore, the prognostic value of thymidylate synthase (TS)-gene, thymidine phosphorylase (TP)-gene, and dihydropyrimidine ... Thymidylate Synthase, Thymidine Phosphorylase, Dihydropyrimidine Dehydrogenase Expression, and Histological Tumour Regression ...https://www.jove.com/author/Gustavo+B._Baretton
Transgenic Plasmodium parasites stably expressing Plasmodium vivax dihydrofolate reductase-thymidylate synthase as in vitro and...
Gene synthesis, expression, and anti-folate-resistant mutants. J Biol Chem. 1993, 268 (29): 21637-21644.PubMedGoogle Scholar. ... Amino acid changes linked to pyrimethamine resistance in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium ... Defining the role of mutations in Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene using an episomal ... Transgenic Plasmodium parasites stably expressing Plasmodium vivax dihydrofolate reductase-thymidylate synthase as in vitro and ...https://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-10-291
CiNii 論文 - Expression of Thymidylate Synthase in Human Non-small Cell Lung Cancer ...
Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human ... Expression of Thymidylate Synthase in Human Non-small Cell Lung Cancer * * OTAKE Yosuke ... The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer ... Elevated expression of thymidylate synthase in doxorubicin resistant human non small cell lung carcinomas FOLM M. ...https://ci.nii.ac.jp/naid/10008093652
Detection of Circulating Cancer Cells with von Hippel-Lindau Gene Mutation in Peripheral Blood of Patients with Renal Cell...
Quantitation of thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene expression in human tumors using the ... Detection of VHL gene mutations in peripheral blood. A, VHL gene mutation was detected in peripheral blood within 24 h after ... It was recently reported that MN/CA9 might be useful as a diagnostic marker for RCCs (18 , 19) . The VHL tumor suppressor gene ... Tada M., Omata M., Kawai S., Saisho H., Ohto M., Saiki R. K., Sninsky J. J. Detection of ras gene mutations in pancreatic juice ...http://clincancerres.aacrjournals.org/content/6/10/3817
Screening of Pleural Mesotheliomas for DNA-damage Repair Players by Digital Gene Expression Analysis Can Enhance Clinical...
Thymidylate synthase and excision repair cross-complementing group-1 as predictors of responsiveness in mesothelioma patients ... Similarly, lower NTHL1 gene expression (p=0.0478) and higher XRCC3 gene expression levels were significantly associated with ... CDC25A gene expression decreases in lymph-metastatic tumours whereas PARP1 gene expression increases in those. Interestingly, ... gene expression associated with OS and PFS in platinum-treated MPM patients . In concordance, CDKN2A gene expression is ...http://www.jcancer.org/v07p1915.htm
Collaborative Advanced Stage Tissue Lung Cancer (CASTLE) Network - Full Text View - ClinicalTrials.gov
... thymidylate synthase), ERCC1 (excision repair cross-complementing 1) and RRM1 (Ribonucleotide Reductase, M1 Subunit) gene ... expressions. *serum: proteomics predictive for EGFR-TKI (tyrosine kinase inhibotors)response. Other: therapeutic interventions ... Enable the discovery of novel genes and proteins related to cancer and its therapies [ Time Frame: 7 years ]. Obtain funding ... tumor: epidermal growth factor receptor (EGFR), KRAS (Kirsten RAt Sarcoma) gene and EML4-ALK (echinoderm microtubule-associated ...https://clinicaltrials.gov/ct2/show/NCT01574300?recr=Open&cond=%22Carcinoma%2C+Bronchogenic%22&rank=9
Response or Resistance to Chemotherapy in Young Patients With Acute Lymphoblastic Leukemia Treated With Methotrexate
... gene expression and gene sequence. alterations using reverse transcriptase-polymerase chain reaction (RT-PCR), thymidylate. ... synthase inhibition assay, Rnase protection assay, or 5'RACE. Multidrug resistance proteins. are also studied by RT-PCR.. A ... Correlate hRFC expression in these specimens with methotrexate transport and. sensitivities.. - Determine the roles of high ... frequency gene/transcript variants for hRFC as determinants. of response and resistance to methotrexate in these patients.. - ...http://www.knowcancer.com/cancer-trials/NCT00898404/
Hua D, Huang ZH*, Deng JZ, Mao Y.Thymidylate synthase and thymidine phosphorylase gene expression as predictive parameters for ... Bian Z#,Yu Y#, Quan C, Guan R, Jin Y, Wu J, Xu L, Chen F, Bai J, Sun W, Fu S.RPL13A as a reference gene for normalizing mRNA ... Bian Z#，Yu Y，Yang T，Quan C，Sun W，Fu S*，Effect of tumor suppressor gene cyclin-dependent kinase inhibitor 2A wild-type and A148T ... Hua D#, Hu Y, Wu YY, Cheng ZH, Yu J, Du X, Huang ZH*.Quantitative methylation analysis of multiple genes using methylation- ...http://wxms.jiangnan.edu.cn/info/1028/2531.htm
Kaposi's sarcoma-associated herpesvirus - Wikipedia
... thymidylate synthase ORF72 - vCyclin ORF73 - LANA - tethers genome to chromosome during latency, also regulates host gene ... viral transcription factors required for the expression of late genes ORF21 - vTK - thymidine kinase ORF22 - gH - envelope ... The "K" genes are unique to KSHV, Some KSHV genes have well-characterized functions, while others remain uncharacterized. ORF2 ... KSHV is a rhadinovirus, and is remarkable since it has stolen numerous genes from host cells including genes that encode for ...https://en.wikipedia.org/wiki/Kaposi's_sarcoma-associated_herpesvirus
Bevacizumab and Oxaliplatin Combined With Irinotecan or Leucovorin and Fluorouracil in Treating Patients With Metastatic or...
Phase II Study of Treatment Selection Based Upon Tumor Thymidylate Synthase Expression in Previously Untreated Patients With ... Correlate gene expression with toxicity of these regimens in these patients.. *Correlate dihydropyrimidine dehydrogenase, ... Patients are stratified according to thymidylate synthase (TS) expression levels (high vs low or indeterminate). Patients with ... Correlate gene expression with response rates in patients treated with these regimens. ...https://clinicaltrials.gov/ct2/show/record/NCT00098787?term=E4203
4SC Announces Positive Interim Results from Clinical Phase I/II SHORE Study
It was also shown that resminostat - due to its gene regulatory properties - decreases the expression of the enzyme thymidylate ... synthase.. This enzyme triggers resistance mechanisms that target the compound 5-FU, which is an integral part of FOLFIRI. ... which can exert a substantial influence on key gene expressions - with traditional chemotherapy such as FOLFIRI, has been ... Missing Hormone Gene Linked to Incurable Prostate Cancer News Jan 17, 2018 ...https://www.technologynetworks.com/drug-discovery/news/4sc-announces-positive-interim-results-from-clinical-phase-iii-shore-study-184567
Frontiers | Nanodrug delivery in reversing multidrug resistance in cancer cells | Pharmacology
... gene expressions by siRNA and miRNA, modulating ceramide levels and targeting NF-B. ... over expression of target enzymes such as up-regulated thymidylate synthase (h) altered drug targets such as topoisomerase II ( ... and resistance to cancer treatments because over-expression of anti-apoptotic genes and under-expression of pro-apoptotic genes ... hypoxia up-regulated expression of MDR-linked genes such as ABC transporters, Bcl-2 family genes, glutathione, metallothionein ...https://www.frontiersin.org/articles/10.3389/fphar.2014.00159/full
Thymidylate kinase - Wikipedia
... gene expression and enzymatic activity coinciding with cell cycle progression and cell growth". DNA Cell Biol. 13 (5): 461-471 ... Predicted thymidylate kinase, TKRP1 InterPro: IPR014505 DTYMK; Thymidine kinase Thymidylate synthase Thymidine kinase in ... Thymidylate kinase (EC 188.8.131.52; dTMP kinase) catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form ... diphosphate Thymidylate kinase is a ubiquitous enzyme of about 25 Kd and is important in the dTTP synthesis pathway for DNA ...https://en.wikipedia.org/wiki/Thymidylate_kinase
Gene expression (quantitative assessment) of genes that regulate the response of cancer cells to chemotherapy drugs
The product of thymidylate synthase (TYMS) gene catalyzes the methylation of deoxyuridylate to deoxythymidylate and is critical ... Gene expression (quantitative assessment) of genes that regulate the response of cancer cells to chemotherapy drugs ... The expression of this gene has been associated with response to gemcitabine chemotherapy. High expression of RRM2 is ... Gene expression (quantitative assessment) of genes that regulate the response of cancer cells to chemotherapy drugs ...http://molecular-view.com/en/services/prognosis-diagnosis/52-gene-expression-quantitative-assessment-of-genes-that-regulate-the-response-of-cancer-cells-to-chemotherapy-drugs.html
Analysis of Origin and Protein-Protein Interaction Maps Suggests a Distinct Oncogenic Role of Nuclear EGFR during Cancer...
... gene expression profiling and EGFR protein expression in primary prostate cancer. BMC cancer. 2011;11:1-12 ... thymidylate synthase, BCRP, and STAT1 (9,11,13,14,16,17). Interestingly, nEGFR does not contain a DNA-binding domain but can ... Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification. ... Nuclear EGFR protein expression predicts poor survival in early stage non-small cell lung cancer. Lung Cancer. 2013;81:138-141 ...http://jcancer.org/v08p0903.htm
Molecular Profiling in Lung Cancer Patients
High/Low Expression of Selected Molecular Markers in Tumor Tissues and Hypermethylated Genes in Peripheral Blood ... Molecular markers assessed by immunohistochemistry: thymidylate synthase, glycinamide ribonucleotide formyl transferase (GARFT ... Hypermethylated genes assessed by methylation-specific polymerase chain reaction. Due to small sample size, tumor-tissue ...http://www.knowcancer.com/cancer-trials/NCT00191308/
... genes with negative Spearman r in Supplementary Table S2). Importantly, thymidylate synthase (TYMS), the direct target of the ... Associations between gene expression and growth of colorectal cancer cells. A, clustering analysis of the 40 genes (rows) whose ... was among the top 50 genes with highest negative correlation between doubling time and gene expression (Fig. 4A). Because of ... To investigate whether there were gene sets with significant enrichment in the number of genes with expression/proliferation ...http://clincancerres.aacrjournals.org/content/21/16/3695
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