Low level of thymidylate synthase gene expression in tumor tissues is associated with response to preoperative...
The gene expression level of thymidylate synthase (TYMS) was significantly higher in nonresponders than in responders (p = ... Low level of thymidylate synthase gene expression in tumor tissues is associated with response to preoperative ... 584P - Low level of thymidylate synthase gene expression in tumor tissues is associated with response to preoperative ... The TYMS gene expression levels in tumor tissues may be useful for predicting the efficacy of preoperative CRT including S-1 or ...https://oncologypro.esmo.org/Topics/Surgery-and-or-Radiotherapy-of-Cancer/Low-level-of-thymidylate-synthase-gene-expression-in-tumor-tissues-is-associated-with-response-to-preoperative-chemoradiotherapy-including-S-1-or-UFT-in-patients-with-rectal-cancer
Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of...
Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human ... Histone deacetylase inhibitors suppress thymidylate synthase gene expression and synergize with the fluoropyrimidines in colon ... Prognostic importance of thymidylate synthase expression in early breast cancer. J Clin Oncol. 1997;15(5):1923-31.CrossRef ... 5-FU upregulated thymidylate synthase, whereas SAHA downregulated its expression. Our results indicate that SAHA and 5-FU act ...https://link.springer.com/article/10.1007/s13277-015-3497-9
A Novel Single Nucleotide Polymorphism within the 5′ Tandem Repeat Polymorphism of the Thymidylate Synthase Gene Abolishes USF...
Thymidylate synthase (TS) gene expression is modulated by a polymorphism in the 5′ regulatory region of the gene. The ... of a polymorphic repeat sequence in the thymidylate synthase gene affect translational efficiency but not its gene expression. ... Polymorphic tandem repeats in the thymidylate synthase gene is associated with its protein expression in human gastrointestinal ... Human thymidylate synthase gene: isolation of phage clones which cover a functionally active gene and structural analysis of ...http://cancerres.aacrjournals.org/content/63/11/2898
Thymidylate Synthase as a Predictor of Response | Cancer Network | The Oncology Journal
... gene expression might be used to select therapy for patients with disseminated colorectal cancer. We recently 1 ... It has been hypothesized that intratumoral thymidylate synthase (TS) ... intratumoral thymidylate synthase (TS) gene expression might be used. to select therapy for patients with disseminated ... thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene. expression in human tumors using the polymerase chain ...http://www.cancernetwork.com/review-article/thymidylate-synthase-predictor-response
Determinants of prognosis and response to therapy in colorectal cancer | SpringerLink
Johnston PG, Lenz H-J, Leichman CG, et al.: Thymidylate synthase protein and gene expression predicts for response to 5- ... Low gene expression levels of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), and thymidine phosphorylase (TP ... Lenz H-J, Leichman CG, Danenberg KD, et al.: Thymidylate synthase expression in adenocarcinoma of the stomach: a predictor for ... Lenz H-J, Danenberg KD, Leichman CG, et al.: P53 and thymidylate synthase expression in untreated stage II colon cancer: ...https://link.springer.com/article/10.1007%2Fs11912-001-0008-5
Thymidylate Synthase and Methylenetetrahydrofolate Reductase Gene Polymorphisms and Toxicity to Capecitabine in Advanced...
Bathe OF, Franceschi D, Livingstone AS, Moffat FL, Tian E, Ardalan B. Increased thymidylate synthase gene expression in liver ... Thymidylate synthase gene polymorphism determines response and toxicity of 5-FU chemotherapy. Pharmacogenomics J 2001;1:65-70. ... A common insertion/deletion polymorphism of the thymidylate synthase (TYMS) gene is a determinant of red blood cell folate and ... Thymidylate synthase gene polymorphism predicts toxicity in colorectal cancer patients receiving 5-fluorouracil-based ...http://clincancerres.aacrjournals.org/content/14/3/817
Expression of uracil DNA glycosylase does not affect cellular sensitivity to thymidylate synthase (TS) inhibition. Eur J Cancer ... Colorectal tumours responding to 5-fluorouracil have low gene expression levels of dihydropyrimidine dehydrogenase, thymidylate ... thymidylate synthase. Introduction. 5-Fluorouracil (FU) is widely used in the treatment of a range of cancers, particularly ... Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim Biophys Acta 2002; 1587: 194-205. ...http://cancerres.aacrjournals.org/content/67/3/940
UCN-01 suppresses thymidylate synthase gene expression and enhances 5-fluorouracil-induced apoptosis in a sequence-dependent ... Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene. Genes Dev 2001;15:2203-8. ... Lack of PTEN expression in non-small cell lung cancer could be related to promoter methylation. Clin Cancer Res 2002;8:1178-84. ... Nonetheless, gene therapy with wild-type PTEN has been done in vivo in a mouse model of lung cancer in which aerosolized PTEN ...http://clincancerres.aacrjournals.org/content/12/3/679
Thymidylate synthase - Wikipedia
2002). "Thymidylate synthase as a translational regulator of cellular gene expression". Biochim. Biophys. Acta. 1587 (2-3): 174 ... Pyrimidine analogues Thymidylate synthase inhibitor Thymidine kinase Thymidine kinase in clinical chemistry Thymidylate kinase ... "Structural and functional analysis of the human thymidylate synthase gene". J. Biol. Chem. 265 (33): 20277-20284. PMID 2243092 ... Thymidylate synthase is an enzyme of about 30 to 35 kDa in most species except in protozoan and plants where it exists as a ...https://en.wikipedia.org/wiki/Thymidylate_synthase
CiNii 論文 - Expression of Thymidylate Synthase in Human Non-small Cell Lung Cancer ...
Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human ... Expression of Thymidylate Synthase in Human Non-small Cell Lung Cancer * * OTAKE Yosuke ... The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer ... Elevated expression of thymidylate synthase in doxorubicin resistant human non small cell lung carcinomas FOLM M. ...https://ci.nii.ac.jp/naid/10008093652
Protocols and Video Articles Authored by Gustavo B. Baretton
To investigate the predictive value of Ki67 and p53 and their correlation with thymidylate synthase (TS) gene expression in a ... Posttherapeutical TS-gene, TP-gene, and DPD-gene expression on surgical resection specimens was quantified by TaqMan real-time ... Furthermore, the prognostic value of thymidylate synthase (TS)-gene, thymidine phosphorylase (TP)-gene, and dihydropyrimidine ... Thymidylate Synthase, Thymidine Phosphorylase, Dihydropyrimidine Dehydrogenase Expression, and Histological Tumour Regression ...https://www.jove.com/author/Gustavo+B._Baretton
Transgenic Plasmodium parasites stably expressing Plasmodium vivax dihydrofolate reductase-thymidylate synthase as in vitro and...
Gene synthesis, expression, and anti-folate-resistant mutants. J Biol Chem. 1993, 268 (29): 21637-21644.PubMedGoogle Scholar. ... Amino acid changes linked to pyrimethamine resistance in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium ... Defining the role of mutations in Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene using an episomal ... Transgenic Plasmodium parasites stably expressing Plasmodium vivax dihydrofolate reductase-thymidylate synthase as in vitro and ...https://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-10-291
Cancer cell adaptation to chemotherapy | BMC Cancer | Full Text
In 8/16 paired esophageal biopsies, there was an increase in the expression of multi-drug resistance gene 1 (MDR1) following ... This study suggests that up-regulation of resistance genes or down-regulation in target genes may occur rapidly in human solid ... epirubicin + cisplatin + 5-fluorouracil (ECF) chemotherapy and this was accompanied by increased expression of the MDR-1 ... or down-regulation of 16 different resistance/target genes, and when tissue was available, immunohistochemistry was used to ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-5-78
Role of Rebiopsy in Relapsed Non-Small Cell Lung Cancer for Directing Oncology Treatments
J. N. Jakobsen, E. Santoni-Rugiu, and J. B. Sørensen, "Thymidylate synthase protein expression levels remain stable during ... Chemotherapy and Gene Expression. The prospective study assessed if chemotherapy selection based on in situ excision repair ... They discovered that thymidylate synthase (TS), which was a potential predictive marker for treatment efficacy with pemetrexed ... Two articles focused on a specific gene expression, while the remaining six focused on tyrosine kinase (TK) resistance and ...https://www.hindawi.com/journals/jo/2015/809835/
Detection of Circulating Cancer Cells with von Hippel-Lindau Gene Mutation in Peripheral Blood of Patients with Renal Cell...
Quantitation of thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene expression in human tumors using the ... Detection of VHL gene mutations in peripheral blood. A, VHL gene mutation was detected in peripheral blood within 24 h after ... It was recently reported that MN/CA9 might be useful as a diagnostic marker for RCCs (18 , 19) . The VHL tumor suppressor gene ... Tada M., Omata M., Kawai S., Saisho H., Ohto M., Saiki R. K., Sninsky J. J. Detection of ras gene mutations in pancreatic juice ...http://clincancerres.aacrjournals.org/content/6/10/3817
Response or Resistance to Chemotherapy in Young Patients With Acute Lymphoblastic Leukemia Treated With Methotrexate
... gene expression and gene sequence. alterations using reverse transcriptase-polymerase chain reaction (RT-PCR), thymidylate. ... synthase inhibition assay, Rnase protection assay, or 5'RACE. Multidrug resistance proteins. are also studied by RT-PCR.. A ... Correlate hRFC expression in these specimens with methotrexate transport and. sensitivities.. - Determine the roles of high ... frequency gene/transcript variants for hRFC as determinants. of response and resistance to methotrexate in these patients.. - ...http://www.knowcancer.com/cancer-trials/NCT00898404/
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thymidylate synthase (TS) is a key enzyme responsible for DNA synthesis and repair. Altered expression of TS protein or TS gene ... Association of thymidylate synthase gene 3-untranslated region polymorphism with sensitivity of non-small cell lung cancer to ... GFP expression was detected by fluoroscopy and flow cytometry (FCM). TK gene expression was detected using RT-PCR and western ... Suicide gene therapy for hepatocellular carcinoma cells by survivin promoter-driven expression of the herpes simplex virus ...https://www.jove.com/visualize?author=Yanyun+Wang
Screening of Pleural Mesotheliomas for DNA-damage Repair Players by Digital Gene Expression Analysis Can Enhance Clinical...
Thymidylate synthase and excision repair cross-complementing group-1 as predictors of responsiveness in mesothelioma patients ... Similarly, lower NTHL1 gene expression (p=0.0478) and higher XRCC3 gene expression levels were significantly associated with ... CDC25A gene expression decreases in lymph-metastatic tumours whereas PARP1 gene expression increases in those. Interestingly, ... gene expression associated with OS and PFS in platinum-treated MPM patients . In concordance, CDKN2A gene expression is ...http://www.jcancer.org/v07p1915.htm
Collaborative Advanced Stage Tissue Lung Cancer (CASTLE) Network - Full Text View - ClinicalTrials.gov
... thymidylate synthase), ERCC1 (excision repair cross-complementing 1) and RRM1 (Ribonucleotide Reductase, M1 Subunit) gene ... expressions. *serum: proteomics predictive for EGFR-TKI (tyrosine kinase inhibotors)response. Other: therapeutic interventions ... Enable the discovery of novel genes and proteins related to cancer and its therapies [ Time Frame: 7 years ]. Obtain funding ... tumor: epidermal growth factor receptor (EGFR), KRAS (Kirsten RAt Sarcoma) gene and EML4-ALK (echinoderm microtubule-associated ...https://clinicaltrials.gov/ct2/show/NCT01574300?recr=Open&cond=%22Carcinoma%2C+Bronchogenic%22&rank=9
Virtual fragment screening for novel inhibitors
Hsueh C. T. , Kelsen D. and Schwartz G. K.UCN\01 suppresses thymidylate synthase gene expression and enhances 5\fluorouracil\ ... Gene expressions had been determined in liver organ and adipose cells. CBX considerably inhibited 11-HSD1 activity in liver ... In prostate cancers perhaps one of the most often mutated/removed genes may be the tumor suppressor gene phosphatase and tensin ... and Seno T.Nucleotide series of an operating cDNA for individual thymidylate synthase . Nucleic Acids Res. , 13 , 2035 C 2045 ...http://code3systems.com
Kaposi's sarcoma-associated herpesvirus - Wikipedia
... thymidylate synthase ORF72 - vCyclin ORF73 - LANA - tethers genome to chromosome during latency, also regulates host gene ... viral transcription factors required for the expression of late genes ORF21 - vTK - thymidine kinase ORF22 - gH - envelope ... The "K" genes are unique to KSHV, Some KSHV genes have well-characterized functions, while others remain uncharacterized. ORF2 ... KSHV is a rhadinovirus, and is remarkable since it has stolen numerous genes from host cells including genes that encode for ...https://en.wikipedia.org/wiki/Kaposi's_sarcoma-associated_herpesvirus
4SC Announces Positive Interim Results from Clinical Phase I/II SHORE Study
It was also shown that resminostat - due to its gene regulatory properties - decreases the expression of the enzyme thymidylate ... synthase.. This enzyme triggers resistance mechanisms that target the compound 5-FU, which is an integral part of FOLFIRI. ... which can exert a substantial influence on key gene expressions - with traditional chemotherapy such as FOLFIRI, has been ... Missing Hormone Gene Linked to Incurable Prostate Cancer News Jan 17, 2018 ...https://www.technologynetworks.com/drug-discovery/news/4sc-announces-positive-interim-results-from-clinical-phase-iii-shore-study-184567
An essential role for water in an enzyme reaction mechanism: the crystal structure of the thymidylate synthase mutant E58Q. -...
Gene. *Gene Expression Omnibus (GEO) Database *Gene Expression Omnibus (GEO) Datasets. *Gene Expression Omnibus (GEO) Profiles ... An essential role for water in an enzyme reaction mechanism: the crystal structure of the thymidylate synthase mutant E58Q.. ... appears to play an important role in the thymidylate synthase (TS) reaction mechanism. We have addressed the role of glutamate ...https://www.ncbi.nlm.nih.gov/pubmed/8973201?dopt=Abstract
Thymidylate kinase - Wikipedia
... gene expression and enzymatic activity coinciding with cell cycle progression and cell growth". DNA Cell Biol. 13 (5): 461-471 ... Predicted thymidylate kinase, TKRP1 InterPro: IPR014505 DTYMK; Thymidine kinase Thymidylate synthase Thymidine kinase in ... Thymidylate kinase (EC 126.96.36.199; dTMP kinase) catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form ... diphosphate Thymidylate kinase is a ubiquitous enzyme of about 25 Kd and is important in the dTTP synthesis pathway for DNA ...https://en.wikipedia.org/wiki/Thymidylate_kinase
Hua D, Huang ZH*, Deng JZ, Mao Y.Thymidylate synthase and thymidine phosphorylase gene expression as predictive parameters for ... Bian Z#,Yu Y#, Quan C, Guan R, Jin Y, Wu J, Xu L, Chen F, Bai J, Sun W, Fu S.RPL13A as a reference gene for normalizing mRNA ... Bian Z#，Yu Y，Yang T，Quan C，Sun W，Fu S*，Effect of tumor suppressor gene cyclin-dependent kinase inhibitor 2A wild-type and A148T ... Hua D#, Hu Y, Wu YY, Cheng ZH, Yu J, Du X, Huang ZH*.Quantitative methylation analysis of multiple genes using methylation- ...http://wxms.jiangnan.edu.cn/info/1028/2531.htm