*  Biomedicines | Free Full-Text | Decoding the Pluripotency Network: The Emergence of New Transcription Factors | HTML
Nanog and Sall4 co-occupied both Nanog and Sall4 enhancer regions in ESCs and activated the gene expression [102]. The direct ... Similar to the Oct4-Sox2 element, the Esrrb-Sox2 element possesses a spacer of 2 to 8 bp [45]. A recent study demonstrated that ... Genetic ablation of GCNF caused embryonic lethality in mice, in which Oct4 expression was no longer restricted to the germ cell ... Genes Cells 2009, 14, 683-694. [Google Scholar] [CrossRef]. *Yang, J.; Corsello, T.R.; Ma, Y. Stem cell gene SALL4 suppresses ...
  http://www.mdpi.com/2227-9059/1/1/49/htm
*  ASMscience | Transposable Elements as
The host variation associated with transposable elements can result from several interconnected aspects of transposable element ... Some features of both transposable elements and hosts suggest coadaptations to mitigate the reduction of fitness expected from ... Two mechanisms are considered most likely to be responsible for transposable element-induced karyotypic changes. The best known ... Estimates of the frequencies of new transposable element-induced mutations have been made under laboratory conditions and ...
  http://www.asmscience.org/content/book/10.1128/9781555817954.chap5
*  The genomic landscape shaped by selection on transposable elements across 18 mouse strains | Genome Biology | Full Text
It is thus plausible that TEVs contribute substantially to the genetic load and gene expression variation among inbred and wild ... effects of TE insertions include their regulation of transcription by acting as alternative promoters or as enhancer elements ... Transposable element (TE)-derived sequence dominates the landscape of mammalian genomes and can modulate gene function by ... Warnefors M, Pereira V, Eyre-Walker A: Transposable elements: insertion pattern and impact on gene expression evolution in ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2012-13-6-r45
*  Short DNA sequence patterns accurately identify broadly active human enhancers | BMC Genomics | Full Text
EnhancerDinucleotide repeat motifMachine learningGene expression. Background. Enhancers are DNA regulatory elements distal to ... An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Sci. 2013;342(6155):253-7.View ... Enhancers are DNA regulatory elements that influence gene expression. There is substantial diversity in enhancers' activity ... For consistency with the enhancer prediction analyses, we analyzed 600 bp regions centered on each enhancer for all enhancer ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3934-9
*  Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine-specific CANNTG motif of the calcitonin...
... cell line TT is modulated by a neuroendocrine-specific enhancer fragment (nucleotides -965 to -905) containing two CANNTG ... Enhancer Elements, Genetic*. Gene Expression Regulation, Neoplastic*. Hela Cells. Humans. Molecular Sequence Data. ... In CT-negative MTC cells (RO-D81), the Ets response element was active but the two copies of E2 were not. Similar results were ... Previous Document: Identification of positive and negative regulatory elements involved in the retinoic acid/cAMP induc.... ...
  http://www.biomedsearch.com/nih/Modified-binding-proteins-from-calcitonin/8265349.html
*  Pluripotential reprogramming of the somatic genome in hybrid cells occurs with the first cell cycle.
Oct4-green fluorescent protein (GFP) transgenes that do not contain the proximal enhancer (PE) region are widely used to ... Enhancer Elements, Genetic. Female. Gene Expression Regulation. Genome. Green Fluorescent Proteins / genetics. Hybrid Cells / ... In addition, demethylation of the Oct4-GFP transgene and that of the endogenous Oct4 and Nanog genes was found to occur within ... We asked whether activation of the transgene actually reflects activation of the endogenous Oct4 gene. In the current study, we ...
  http://www.biomedsearch.com/nih/Pluripotential-reprogramming-somatic-genome-in/18065396.html
*  Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2 | Cancer Research
Schmitt C. A., Rosenthal C. T., Lowe S. W. Genetic analysis of chemoresistance in primary murine lymphomas. Nat. Med., 6: 1029- ... Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2. Caroline A. Heckman, ... Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2 ... Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2 ...
  http://cancerres.aacrjournals.org/content/63/20/6666.long
*  Genetic Basis of Hypertension | Hypertension
Nibu Y, Takahashi S, Tanimoto K, Murakami K, Fukamizu A. Identification of cell type-dependent enhancer core element located in ... The human AGT gene, a member of the serpin gene superfamily, stretches over only &12 kb on chromosome 1 (1q42-q43) and contains ... However, the most compelling early genetic evidence implicating this gene in essential hypertension in humans came from the ... The AGT Gene and Protein. Among candidate genes for essential hypertension, AGT was the first and remains perhaps "the most ...
  http://hyper.ahajournals.org/content/48/1/14.long
*  Limitations of the murine nose in the development of nonviral airway gene transfer. - Radcliffe Department of Medicine
The plasmids used were regulated by either (1) the commonly used short-acting cytomegalovirus promoter/enhancer or (2) the ... lung disease is currently being undertaken by the UK CF Gene Therapy Consortium. We have evaluated GL67A gene transfer to the ... At the current levels of gene transfer efficiency achievable with nonviral vectors, the murine nose is of limited value as a ... We then assessed whether CFTR gene transfer corrected a panel of CFTR-specific endpoint assays in the murine nose, including ...
  https://www.rdm.ox.ac.uk/publications/116955
*  "The chemotherapeutic agent DMXAA potently and specifically activates t" by Zachary J. Roberts, Nadege Goutagny et al.
... activation and elicited minimal nuclear factor kappaB-dependent gene expression. DMXAA-induced signaling was critically ... Animals; Antineoplastic Agents; Cells, Cultured; Cytokines; DNA; Enhancer Elements, Genetic; Female; Gene Expression Regulation ... activation and elicited minimal nuclear factor kappaB-dependent gene expression. DMXAA-induced signaling was critically ...
  https://escholarship.umassmed.edu/infdis_pp/79/
*  Medical Xpress - genetic mutations(... continued page 8)
Genetic mutations can increase a person's cancer risk, but other gene "enhancer" elements may also be responsible for disease ... Research asks why genetic mutation leads to decreased triglycerides in blood. Common knowledge says that genetic mutations are ... Genetic profile of treatment-resistant lung cancer more variable than previously thought. The genetic mutations underlying ... Researchers find gene Wt1 to impact women's fertility. It has been estimated that more than 80 million people in the world have ...
  https://medicalxpress.com/tags/genetic+mutations/page8.html
*  Imaging of flexor and extensor motoneurons targeted wit | Open-i
Enhancer Elements, Genetic. *Genes, Reporter. *Integrases/genetics. *Motor Neurons/cytology. *Rhodopsin/metabolism ... Bottom Line: Here we present a circuit-deciphering 'tool box' for fast, reliable and cheap genetic targeting of neuronal ... Bottom Line: Here we present a circuit-deciphering 'tool box' for fast, reliable and cheap genetic targeting of neuronal ... with ubiquitously-expressed PBase and Cre under the control of the chick motoneuron-specific enhancer of the TAG1/Axonin1 gene ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4231727_gku750fig6&req=4
*  Patent US6242570 - Production and use of recombinant protein multimers with increased ... - Google Patents
... a genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers; (2) a ... Gene 42:133, 1986); Bauer et al. (Gene 37:73, 1985); Craik (BioTechniques, Jan. 12-19, 1985); Smith et al. (Genetic Engineering ... the ampicillin resistance gene of E. coli and S. cerevisiae trpl gene, which provides a selection marker for a mutant strain of ... viral or insect genes. Such regulatory elements include a transcriptional promoter, an optional operator sequence to control ...
  http://www.google.com/patents/US6242570
*  piRNAs, transposon silencing, and Drosophila germline development | JCB
Mobile genetic elements also carry transcriptional enhancers and insulators, thus transposition can alter expression of nearby ... Genetic investigation of cAMP-dependent protein kinase function in Drosophila development. Genes Dev. 7(7A):1229-1243. doi: ... A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 313:320-324. doi:10.1126/science. ... Unique germ-line organelle, nuage, functions to repress selfish genetic elements in Drosophila melanogaster. Proc. Natl. Acad. ...
  http://jcb.rupress.org/content/191/5/905
*  The diverse evolutionary pattern of ESEs and ESSs in as | Open-i
Enhancer Elements, Genetic. *Gene Silencing. *Genetic Variation. *Humans. *Mice. *Models, Genetic. *Models, Statistical ... Divergence of exonic splicing elements after gene duplication and the impact on gene structures. ... Divergence of exonic splicing elements after gene duplication and the impact on gene structures. ... Results: We studied the divergence of exonic splicing enhancers and silencers after gene duplication and the contributions of ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC3091315_gb-2009-10-11-r120-3&req=4
*  Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. - Radcliffe...
The α-globin enhancers now interact with the flanking chromatin, upregulating expression of genes within this extended sub-TAD ... Whereas the α-globin regulatory elements normally act solely on promoters downstream of the enhancers, removal of a conserved ... containing the mouse α-globin gene cluster, lying within a ∼1 Mb TAD. We find that the sub-TAD is flanked by predominantly ... CTCF-cohesin boundaries in this sub-TAD delimit the region of chromatin to which enhancers have access and within which they ...
  https://www.rdm.ox.ac.uk/publications/709225
*  ZFIN Publication: Lee et al., 2015
Enhancer Elements, Genetic*; Epigenesis, Genetic; Female; Gene Expression Regulation, Developmental*; Gene Library; Gene ... are associated with developmental genes and are marked with active enhancer histone posttranslational modifications. Their ... Lee, H.J., Lowdon, R.F., Maricque, B., Zhang, B., Stevens, M., Li, D., Johnson, S.L., Wang, T. (2015) Developmental enhancers ... However, developmental DNA methylation dynamics of the majority of the genomic CpGs outside gene promoters and CpG islands has ...
  http://zfin.org/ZDB-PUB-150224-20
*  Regulation from a distance | Philosophical Transactions of the Royal Society B: Biological Sciences
... of regulation from elements-termed enhancers-that can be located up to 2 million base pairs away from the affected genes [3]. ... 2013 Human genetic variation within neural crest enhancers: molecular and phenotypic implications. Phil. Trans. R. Soc. B 368, ... gene deserts' a long way from any recognized gene, which makes the recognition of target genes difficult [6]. A proportion is ... 2013 From remote enhancers to gene regulation: charting the genome's regulatory landscapes. Phil. Trans. R. Soc. B 368, ...
  http://rstb.royalsocietypublishing.org/content/368/1620/20120372
*  ZFIN Publication: Gehrke et al., 2015
Enhancer Elements, Genetic; Fishes/anatomy & histology*; Fishes/genetics; Gene Expression Regulation, Developmental; Genes, ... Animals; Enhancer Elements, Genetic; Fishes/anatomy & histology*; Fishes/genetics; Gene Expression Regulation, Developmental ( ... Moreover, gar enhancers drive reporter gene expression in both the wrist and digits of mouse embryos in patterns that are ... In mouse limbs the autopod is built by a "late" phase of Hoxd and Hoxa gene expression, orchestrated by a set of enhancers ...
  http://zfin.org/ZDB-PUB-141224-10
*  CRISPR-based strategies for studying regulatory elements and chromatin structure in mammalian gene control | Springer for...
The development of high-throughput methods has enabled the genome-wide identification of putative regulatory elements in a wide ... Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9. Nat Biotechnol 34:192-198. https://doi. ... Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements. Nat Genet 49:1602-1612. ... rs2279590 lies within an enhancer element and regulates CLU, EPHX2 and PTK2B gene expression. Hum Mol Genet 26:4519-4529. https ...
  https://rd.springer.com/article/10.1007/s00335-017-9727-2
*  Patent US6391637 - Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells - Google Patents
This selectable marker can comprise genetic elements of the cloning vector pBR322 (ATCC 37017). pBR322 contains genes for ... an enhancer linked to the structural gene, other 5′ or 3′ flanking nontranscribed sequences, such as ribosome binding sites, a ... and polyadenylation sites may be used to provide the other genetic elements required for expression of a structural gene ... and constitutively express EBNA-1 driven from human CMV immediate-early enhancer/promoter. An EBNA-1 gene allows for episomal ...
  http://www.google.com.au/patents/US6391637
*  Enhancer evolution and animal diversity | Philosophical Transactions of the Royal Society B: Biological Sciences
2012 Positive selection of co-opted mobile genetic elements in a mammalian gene: if you can't beat them, join them. Mob. Genet ... many developmental genes carry two enhancers with overlapping functions; the enhancer closer to the gene was called 'primary', ... Genetic mechanisms leading to the birth of novel enhancers include: (i) insertion of transposable elements (TEs), (ii) de novo ... 2012 The chromatin fingerprint of gene enhancer elements. J. Biol. Chem. 287, 30 888-30 896. (doi:10.1074/jbc.R111.296491). ...
  http://rstb.royalsocietypublishing.org/content/368/1632/20130017
*  Pleiotropy - Wikipedia
cis-regulatory element Enhancer (genetics) Epistasis Genetic correlation Metabolic network Metabolic supermice Polygene Paaby, ... Pleiotropy describes the genetic effect of a single gene on multiple phenotypic traits. The underlying mechanism is genes that ... Some gene evolution is harmful to an organism. Genetic correlations and responses to selection most often exemplify pleiotropy ... It is caused by a change in one nucleotide, a point mutation in the HBB gene. The HBB gene encodes information to make the beta ...
  https://en.wikipedia.org/wiki/Pleiotropy
*  Noncoding DNA - Wikipedia
A genetic insulator is a boundary element that plays two distinct roles in gene expression, either as an enhancer-blocking code ... Cis-regulatory elements are sequences that control the transcription of a nearby gene. Many such elements are involved in the ... to enhance transcription levels of genes in a gene cluster.. A silencer is a region of DNA that inactivates gene expression ... Enhancer sequences may also exert very distant effects on the transcription levels of genes. Introns are non-coding sections of ...
  https://en.wikipedia.org/wiki/Noncoding_DNA
*  Bromodomain protein 4 discriminates tissue-specific super-enhancers containing disease-specific susceptibility loci in prostate...
Super-enhancers are cell-specific DNA elements, acting to determine tissue or cell identity and driving tumor progression. ... and provides a powerful tool for enriching and prioritizing PC and BC genetic risk loci. Our study represents a proof of ... We find that BRD4 is the key discriminant of tissue-specific enhancers, showing that it is more powerful than AR binding ... RESULTS: Here we establish criteria for enhancer mapping which are applicable to other diseases and traits to achieve the ...
  https://www.ndph.ox.ac.uk/publications/689058