Therapeutic Targeting of ATP7B in Ovarian Carcinoma | Clinical Cancer Research
... the effects of ATP7A or ATP7B silencing were assessed on tumor cell sensitivity to cisplatin using the MTT assay. ATP7A gene ... Copper transport and kinetics in cultured C6 rat glioma cells. Am J Physiol 1995;269:C892-8. ... Cell lines and culture. The derivation, source, and propagation of human epithelial ovarian cancer cell lines, such as ... B, effect of ATP7A and ATP7B silencing on cisplatin sensitivity in ovarian cancer cell lines. A2780-PAR and A2780-CP20 cells ...http://clincancerres.aacrjournals.org/content/15/11/3770.long
Nanaomycin A Selectively Inhibits DNMT3B and Reactivates Silenced Tumor Suppressor Genes in Human Cancer Cells | Molecular...
Cell culture. A549, HL60, and HCT116 cells were obtained directly from the American Type Culture Collection and passaged in our ... Nanaomycin A Selectively Inhibits DNMT3B and Reactivates Silenced Tumor Suppressor Genes in Human Cancer Cells. Dirk Kuck, ... Nanaomycin A Selectively Inhibits DNMT3B and Reactivates Silenced Tumor Suppressor Genes in Human Cancer Cells ... Nanaomycin A Selectively Inhibits DNMT3B and Reactivates Silenced Tumor Suppressor Genes in Human Cancer Cells ...http://mct.aacrjournals.org/content/9/11/3015
Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer Treatment | Clinical Cancer...
Cell culture and primary tumor samples. A total of 52 colorectal cancer cell lines were used: Caco2, Colo201, Colo205, Colo320 ... Cancer Therapy: Preclinical Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer ... Growth of colorectal cancer cell lines. A, for a panel of 52 colorectal cancer cell lines, cells were seeded in seven 96-well ... The doubling time of colorectal cancer cell lines is associated with MSI and tumor grade. A, colorectal cancer cell lines with ...http://clincancerres.aacrjournals.org/content/21/16/3695
Gene expression analysis of bone metastasis and circulating tumor cells from metastatic castrate-resistant prostate cancer...
Low numbers of PC3 prostate cancer (PCa) cells were spiked into normal blood to assess cell recovery rate. RNA extracted from ... Conversely, circulating tumor cells (CTCs), which are likely to stem from bone metastases, can be isolated from blood. The ... through in vitro transcription may be useful as a method to detect gene expression in small numbers of CTCs and tumor cells ... We found that in 21 out of 28 comparisons, the presence or absence of detectable gene expression in CTCs and PCa cells ...https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0829-5
Modus Operandi Articles - cancer, cell & molecular biology, gene silencing and neuroscience | The Scientist Magazine®
New monoclonal antibodies kill both cancer-promoting immunosuppressive cells and tumor cells in culture. ... tags: cancer x cell & molecular biology x gene silencing x neuroscience x ... Combining gene editing and stem-cell induction improves efficiency of functional genetic analyses. ... Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until ...https://www.the-scientist.com/?articles.list/categoryNo/2928/category/Modus-Operandi/tagNo/20,4,1405,11/tags/cancer,cell--amp--molecular-biology,gene-silencing,neuroscience/
Primary breast cancer cell culture yields intra-tumor heterogeneous subpopulations expressing exclusive patterns of receptor...
Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc Natl Acad Sci U S A. 1999;96(16 ... Cell culture. MBCDF primary breast cancer cell culture was previously described . Briefly, a biopsy was obtained from a ... The intra-tumor heterogeneity observed in the MBCDF primary breast cancer cell culture suggests that subpopulations with a ... Primary breast cancer cell culture yields intra-tumor heterogeneous subpopulations expressing exclusive patterns of receptor ...https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2769-0
Itraconazole Inhibits Angiogenesis and Tumor Growth in Non-Small Cell Lung Cancer | Cancer Research
A primary xenograft model of small-cell lung cancer reveals irreversible changes in gene expression imposed by culture in vitro ... Response to epidermal growth factor receptor inhibitors in non-small cell lung cancer cells. Clin Cancer Res 2003;9:2316-26. ... Itraconazole Inhibits Angiogenesis and Tumor Growth in Non-Small Cell Lung Cancer. Blake T. Aftab, Irina Dobromilskaya, Jun O. ... Cultures from LX-7 and LX-14 tumors were derived from preparations of single-cell suspensions grown in Media-2 plus 4.5 g/L ...http://cancerres.aacrjournals.org/content/71/21/6764
Tumor Cells Deactivate Human Monocytes by Up-Regulating IL-1 Receptor Associated Kinase-M Expression via CD44 and TLR4 | The...
Fixed tumor cell lines and soluble factors from cultured cancer cells induce IRAK-M gene expression in human monocytes. To gain ... cultured both in the presence of fixed tumor cells and in medium supplemented with the supernatant from cancer cell cultures. ... as well as by fixed tumor cells and medium supplemented with the supernatant from tumor cell cultures. Moreover, blood ... factors from tumor cell lines by culturing human monocytes in the presence of filtered medium conditioned by cancer cells for ...http://www.jimmunol.org/content/174/5/3032.long
Cultured circulating tumor cells reveal genetic profile of breast cancer cells - ONA
... in order to track ongoing genetic mutation within a tumor. ... created a microchip device that can capture circulating tumor ... Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and ... possible for the study group to were able to screen for mutations in approximately one thousand known cancer-associated genes. ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells Circulating tumor cells captured with a ...http://www.oncologynurseadvisor.com/headlines/cultured-circulating-tumor-cells-reveal-genetic-profile-of-breast-cancer-cells/article/361324/
Generation of tumor-initiating cells by exogenous delivery of OCT4transcription factor | Breast Cancer Research | Full Text
... feeder cultures in human embryonic stem cell media). Differentiation assays, immunofluorescence, immunohistochemistry, and flow ... Gene expression microarrays of OTBC lines revealed a gene signature that was over-represented in the claudin-low molecular ... Transduction of OCT4 in normal breast preparations led to the generation of cell lines possessing tumor-initiating and ... suggesting that the cell of origin of some OTBCs was a bi-potent stem cell. Injection of OTBCs in nude mice generated poorly ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3019
Dietary Chemopreventative Benzyl Isothiocyanate Inhibits Breast Cancer Stem Cells In Vitro and In Vivo | Cancer Prevention...
The BITC treatment downregulated protein levels of Ron and sfRon in cultured breast cancer cells and in tumor xenografts. Ron ... Ron and sfRon induces stemness in MCF-7 cells that is accompanied by increased expression of stem cell-associated genes Oct-4, ... Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. ... A small subset of mammary tumor-initiating cells (also known as breast cancer stem cells; bCSC), characterized by expression of ...http://cancerpreventionresearch.aacrjournals.org/content/6/8/782?iss=8
Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal...
... have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined.... ... Background T cells engineered to express chimeric antigen receptors (CARs) ... T-cell killing of heterogenous tumor or viral targets with bispecific chimeric immune receptors. Cancer Gene Ther. 2000;7:1127- ... T cells were transduced with a gamma-retroviral vector containing the CC49-ζ CAR construct (Fig. 1). All CART72 cell cultures ...https://rd.springer.com/article/10.1186/s40425-017-0222-9
Characterization of ERG, AR and PTEN Gene Status in Circulating Tumor Cells from Patients with Castration-Resistant Prostate...
Circulating tumor cell number and prognosis in progressive castration-resistant prostate cancer. Clin Cancer Res 2007; 13: 7053 ... and normalized to the ERG/rpl32 ratio of VCaP cells cultured in 10% fetal bovine serum (FBS)-containing media. Dextran- ... Circulating tumor cells predict survival benefit from treatment in metastatic castration resistant prostate cancer. Clin Cancer ... AR and PTEN Gene Status in Circulating Tumor Cells from Patients with Castration-Resistant Prostate Cancer. Gerhardt Attard, ...http://cancerres.aacrjournals.org/content/69/7/2912
Gene Knockdown by EpCAM Aptamer-siRNA Chimeras Suppresses Epithelial Breast Cancers and Their Tumor-Initiating Cells |...
EpCAM also marks tumor-initiating cells (TIC, also known as cancer stem cells; refs. 20-27). Although the cancer stem cell ... For sphere formation assay, cells (1,000/mL), treated as above for 6 hours in round-bottom 96-well plates, were cultured in ... PLK1 EpCAM-AsiCs inhibit tumor initiation of EpCAM+ breast cancer cells. A-C, human breast cancer cell lines [luminal (blue), ... We first examined EpCAM expression in breast cancer cell lines. On the basis of gene expression data in the Cancer Cell Line ...http://mct.aacrjournals.org/content/14/10/2279
Structurally Novel Antiestrogens Elicit Differential Responses from Constitutively Active Mutant Estrogen Receptors in Breast...
In-cell Western and Western blot assays. Cells were cultured in 96-well plates at 3,000 cells per well and treated with ... Patients with ER-positive tumors containing breast cancer cells with mutant ERs present in different tumor microenvironments ... Despite the fact that all three AEs showed good antiproliferative and target gene-inhibitory activities in cells in culture, K- ... 17, 21, 23, 24). These cells were cultured as described previously (17, 21, 23, 24). Serum used in all cell cultures was ...http://cancerres.aacrjournals.org/content/77/20/5602
Boswellia sacra essential oil induces tumor cell-specific apoptosis and suppresses tumor aggressiveness in cultured human...
Activation of CDK4 gene expression in human breast cancer cells by the Brn-3b POU family transcription factor. Cancer Biol Ther ... hepatoma cells , melanoma cells, fibrosarcoma cells , colon cancer cells , and prostate cancer cells [20-22]. ... essential oil induces tumor cell-specific apoptosis and suppresses tumor aggressiveness in cultured human breast cancer cells. ... Breast cancer cells were seeded at the concentration of 5 × 105 cells/60 mm tissue culture plate. After adherence, cells were ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-11-129/
Phenotypic Characteristics of Cell Lines Derived from Disseminated Cancer Cells in Bone Marrow of Patients with Solid...
Expansion of tumor cells in BM culture shown for patient LC-m1. The culture was split at ∼70% confluency. Before passage 3, ... Heterogeneous mRNA expression of prostate cancer-associated genes on micrometastatic cancer cell lines (RT-PCR analysis)a ... Tumor Biology Phenotypic Characteristics of Cell Lines Derived from Disseminated Cancer Cells in Bone Marrow of Patients with ... Because of the few tumor cells initially present in each of the BM samples, we analyzed cell lines established from these cells ...http://cancerres.aacrjournals.org/content/59/1/241?ijkey=b43f44d1d22b8000794ddab9b79bb9946ad32559&keytype2=tf_ipsecsha
Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a...
116 human colon cancer cell line. Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter ... Figure legend for Supplementary Figure 1: ABCG2 promoter luciferase reporter gene assay showing that the promoter region ... Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a ...https://www.hindawi.com/journals/isrn/2012/746025/sup/
Comparative Proteomics of Colon Cancer Stem Cells and Differentiated Tumor Cells Identifies BIRC6 as a Potential Therapeutic...
Colorectal Cancer Stem Cells and Differentiated Tumor Cell Cultures. Collection of tumor specimens, isolation and expansion of ... 2006) Analysis of gene expressio! and chemoresistance of CD133+ cancer stem cells in glioblastoma. Mol. Cancer 5, 67. ... "cancer stem cell " fraction of colorectal tumors. Cancer stem cells make up only a few percent of the total tumor cell mass, ... 2007) Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell 1, 389- ...http://www.mcponline.org/content/10/12/M111.011353
ATCC Cell Lines by APC Gene Mutation Page 1
... altered cell migration and chromosome instability. They have been implicated in colon, lung and esophageal cancers. ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cells and Microorganisms * Collections * Focus Area * Disease/Model * * Tissue * Tumor Cell Panels ...https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/APC.aspx?dsNav=N:4294922437
ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cell Type: Melanocyte,Melanoma Tissue: Previously Described As: Mammary Gland/Breast; Derived From Metastatic Site: Pleural ...https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Ro:20
ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cells and Microorganisms * Collections * Focus Area * Disease/Model * * Tissue * Tumor Cell Panels ...https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Up:Page_Product_Listing,N:4294927639
ATCC Cell Lines by APC Gene Mutation Page 1
... altered cell migration and chromosome instability. They have been implicated in colon, lung and esophageal cancers. ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Grade Iii, Mesodermal Tumor (Mixed) ; Consistent With Leiomy... (1) * Grade Iv, Adenocarcinoma (1) ...https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/APC.aspx?slp=1
Exploring Intra-tumor Cooperation in Metastasis and Drug Resistance using Heterogeneous Xenograft Models of Breast Cancer
Using a 3D co-culture model, we identified significant sub-type-specific changes in gene expression, metabolic, and therapeutic ... Lastly, we examined heterotypic interactions between tumor cells and cancer associated fibroblasts (CAFs) to understand the ... Our model revealed that tumor growth can be driven by a minor clone, expressing IL11, in a non-cell autonomous fashion mediated ... as well as between tumor microenvironmental components and cancer cells, to identify mechanisms that influence tumorigenesis, ...https://dash.harvard.edu/handle/1/33493472
ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... GCT [Giant Cell Tumor] (ATCC® TIB-223™) ATCC® Number: TIB-223™ Organism: Homo sapiens, human ...https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Ro:10,Up:Page_Product_Listing&slp=1