*  Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer Treatment | Clinical Cancer...
Cell culture and primary tumor samples. A total of 52 colorectal cancer cell lines were used: Caco2, Colo201, Colo205, Colo320 ... Cancer Therapy: Preclinical Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer ... Growth of colorectal cancer cell lines. A, for a panel of 52 colorectal cancer cell lines, cells were seeded in seven 96-well ... The doubling time of colorectal cancer cell lines is associated with MSI and tumor grade. A, colorectal cancer cell lines with ...
  http://clincancerres.aacrjournals.org/content/21/16/3695
*  Modus Operandi Articles - cancer, cell & molecular biology, gene silencing and neuroscience | The Scientist Magazine®
New monoclonal antibodies kill both cancer-promoting immunosuppressive cells and tumor cells in culture. ... tags: cancer x cell & molecular biology x gene silencing x neuroscience x ... Combining gene editing and stem-cell induction improves efficiency of functional genetic analyses. ... Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until ...
  https://www.the-scientist.com/?articles.list/categoryNo/2928/category/Modus-Operandi/tagNo/20,4,1405,11/tags/cancer,cell--amp--molecular-biology,gene-silencing,neuroscience/
*  Primary breast cancer cell culture yields intra-tumor heterogeneous subpopulations expressing exclusive patterns of receptor...
Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc Natl Acad Sci U S A. 1999;96(16 ... Cell culture. MBCDF primary breast cancer cell culture was previously described [23]. Briefly, a biopsy was obtained from a ... The intra-tumor heterogeneity observed in the MBCDF primary breast cancer cell culture suggests that subpopulations with a ... Primary breast cancer cell culture yields intra-tumor heterogeneous subpopulations expressing exclusive patterns of receptor ...
  https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2769-0
*  Itraconazole Inhibits Angiogenesis and Tumor Growth in Non-Small Cell Lung Cancer | Cancer Research
A primary xenograft model of small-cell lung cancer reveals irreversible changes in gene expression imposed by culture in vitro ... Response to epidermal growth factor receptor inhibitors in non-small cell lung cancer cells. Clin Cancer Res 2003;9:2316-26. ... Itraconazole Inhibits Angiogenesis and Tumor Growth in Non-Small Cell Lung Cancer. Blake T. Aftab, Irina Dobromilskaya, Jun O. ... Cultures from LX-7 and LX-14 tumors were derived from preparations of single-cell suspensions grown in Media-2 plus 4.5 g/L ...
  http://cancerres.aacrjournals.org/content/71/21/6764
*  Tumor Cells Deactivate Human Monocytes by Up-Regulating IL-1 Receptor Associated Kinase-M Expression via CD44 and TLR4 | The...
Fixed tumor cell lines and soluble factors from cultured cancer cells induce IRAK-M gene expression in human monocytes. To gain ... cultured both in the presence of fixed tumor cells and in medium supplemented with the supernatant from cancer cell cultures. ... as well as by fixed tumor cells and medium supplemented with the supernatant from tumor cell cultures. Moreover, blood ... factors from tumor cell lines by culturing human monocytes in the presence of filtered medium conditioned by cancer cells for ...
  http://www.jimmunol.org/content/174/5/3032.long
*  Cultured circulating tumor cells reveal genetic profile of breast cancer cells - ONA
... in order to track ongoing genetic mutation within a tumor. ... created a microchip device that can capture circulating tumor ... Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and ... possible for the study group to were able to screen for mutations in approximately one thousand known cancer-associated genes. ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells Circulating tumor cells captured with a ...
  http://www.oncologynurseadvisor.com/headlines/cultured-circulating-tumor-cells-reveal-genetic-profile-of-breast-cancer-cells/article/361324/
*  Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal...
... have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined.... ... Background T cells engineered to express chimeric antigen receptors (CARs) ... T-cell killing of heterogenous tumor or viral targets with bispecific chimeric immune receptors. Cancer Gene Ther. 2000;7:1127- ... T cells were transduced with a gamma-retroviral vector containing the CC49-ζ CAR construct (Fig. 1). All CART72 cell cultures ...
  https://rd.springer.com/article/10.1186/s40425-017-0222-9
*  Gene Knockdown by EpCAM Aptamer-siRNA Chimeras Suppresses Epithelial Breast Cancers and Their Tumor-Initiating Cells |...
EpCAM also marks tumor-initiating cells (TIC, also known as cancer stem cells; refs. 20-27). Although the cancer stem cell ... For sphere formation assay, cells (1,000/mL), treated as above for 6 hours in round-bottom 96-well plates, were cultured in ... PLK1 EpCAM-AsiCs inhibit tumor initiation of EpCAM+ breast cancer cells. A-C, human breast cancer cell lines [luminal (blue), ... We first examined EpCAM expression in breast cancer cell lines. On the basis of gene expression data in the Cancer Cell Line ...
  http://mct.aacrjournals.org/content/14/10/2279
*  Boswellia sacra essential oil induces tumor cell-specific apoptosis and suppresses tumor aggressiveness in cultured human...
Activation of CDK4 gene expression in human breast cancer cells by the Brn-3b POU family transcription factor. Cancer Biol Ther ... hepatoma cells [17], melanoma cells, fibrosarcoma cells [18], colon cancer cells [19], and prostate cancer cells [20-22]. ... essential oil induces tumor cell-specific apoptosis and suppresses tumor aggressiveness in cultured human breast cancer cells. ... Breast cancer cells were seeded at the concentration of 5 × 105 cells/60 mm tissue culture plate. After adherence, cells were ...
  https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-11-129/
*  ATCC Cell Lines by APC Gene Mutation Page 1
... altered cell migration and chromosome instability. They have been implicated in colon, lung and esophageal cancers. ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cells and Microorganisms * Collections * Focus Area * Disease/Model * * Tissue * Tumor Cell Panels ...
  https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/APC.aspx?dsNav=N:4294922437
*  ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cell Type: Melanocyte,Melanoma Tissue: Previously Described As: Mammary Gland/Breast; Derived From Metastatic Site: Pleural ...
  https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Ro:20
*  ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Cells and Microorganisms * Collections * Focus Area * Disease/Model * * Tissue * Tumor Cell Panels ...
  https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Up:Page_Product_Listing,N:4294927639
*  ATCC Cell Lines by APC Gene Mutation Page 1
... altered cell migration and chromosome instability. They have been implicated in colon, lung and esophageal cancers. ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Grade Iii, Mesodermal Tumor (Mixed) ; Consistent With Leiomy... (1) * Grade Iv, Adenocarcinoma (1) ...
  https://www.atcc.org/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/APC.aspx?slp=1
*  Exploring Intra-tumor Cooperation in Metastasis and Drug Resistance using Heterogeneous Xenograft Models of Breast Cancer
Using a 3D co-culture model, we identified significant sub-type-specific changes in gene expression, metabolic, and therapeutic ... Lastly, we examined heterotypic interactions between tumor cells and cancer associated fibroblasts (CAFs) to understand the ... Our model revealed that tumor growth can be driven by a minor clone, expressing IL11, in a non-cell autonomous fashion mediated ... as well as between tumor microenvironmental components and cancer cells, to identify mechanisms that influence tumorigenesis, ...
  https://dash.harvard.edu/handle/1/33493472
*  ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... GCT [Giant Cell Tumor] (ATCC® TIB-223™) ATCC® Number: TIB-223™ Organism: Homo sapiens, human ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Ro:10,Up:Page_Product_Listing&slp=1
*  ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... This validated panel is composed of 8 select human tumor cell lines carrying BRAF mutations, and is useful for BRAF pathway ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Nrc:id-1000511&hasShowAll=
*  ATCC Cell Lines by BRAF Gene Mutation Page 1
... and are commonly found in birth defects and thyroid and skin cancers. ... BRAF mutations lead to excessive cell proliferation and survival, independent of growth factors, ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Disease: Stage 4,Adenocarcinoma; Non-Small Cell Lung Cancer .plsearchwithin .searchinresults input.text { padding: 7px 4px; ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Disease__Model/Cancer/Tumor_Cell_Panels/Cell_lines_by_genetic_mutation/BRAF.aspx?dsNav=Nrc:id-1000511-dynrank-disabled,N:4294963777&hasShowAll=1000511%7C
*  BRAF Inhibition Increases Tumor Infiltration by T cells and Enhances the Antitumor Activity of Adoptive Immunotherapy in Mice |...
PD-1 blockade enhances T-cell migration to tumors by elevating IFN-gamma inducible chemokines. Cancer Res 2012;72:5209-18. ... then were washed 3 times with culture medium. After counting, tumor cells were coincubated with DMF5 or pmel-1 T cells at 5 × ... Infected tumor cells were collected and sorted on the basis of the expression of the reporter gene using a FACSAria. ... Using gp100-negative tumor cells, PLX4720 treatment failed to increase T-cell trafficking to tumor sites. These findings ...
  http://clincancerres.aacrjournals.org/content/19/2/393?rss=1
*  Modulation of Gene Expression and Cell-Cycle Signaling Pathways by the EGFR Inhibitor Gefitinib (Iressa) in Rat Urinary Bladder...
Squamous cell carcinoma accounts for around 8% of bladder cancer cases. These cancers originate from the thin, flat cells that ... Cell culture study confirmed that the increased expression of let-7c decreases Iressa-treated bladder tumor cell growth. The ... Both tumor module and treatment module are enriched for genes involved in cell-cycle control. The tumor module and treatment ... Cell-culture study also confirmed that the increased expression of let-7c decreases Iressa-treated bladder tumor cell growth. ...
  http://cancerpreventionresearch.aacrjournals.org/content/5/2/248
*  Arsenic trioxide in the mechanism of drug resistance reversal in MCF-7/ADM cell line of human breast cancer.
... and its possible mechanism in human breast cancer cell line MCF-7/ADM. METHODS: Cytotoxicity of As(2)O(3) and the sensibility ... Tumor Cells, Cultured. Chemical. Reg. No./Substance: 0/Antineoplastic Agents; 0/Arsenicals; 0/Isoenzymes; 0/Oxides; 0/RNA, ... Gene Expression. Glutathione S-Transferase pi. Glutathione Transferase / genetics. Humans. Isoenzymes / genetics. Oxides / ... Cadmium uptake and metabolism in cultured cells.. 19322799 - Effects of thymoquinone-fatty acid conjugates on cancer cells.. ...
  http://www.biomedsearch.com/nih/Arsenic-trioxide-in-mechanism-drug/12408759.html
*  Targeting Tumor-Infiltrating Macrophages Decreases Tumor-Initiating Cells, Relieves Immunosuppression, and Improves...
... and endothelial cell/tumor cell interactions may be a critical component of the cancer stem cell niche (44-46). Thus, multiple ... Mean fold changes in CD45−ALDHBright cells is depicted. B, quantitation of PDAC tumor spheroids following 14 days of culture in ... IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion. Genes Dev 2010; ... Marginating dendritic cells of the tumor microenvironment cross-present tumor antigens and stably engage tumor-specific T cells ...
  http://cancerres.aacrjournals.org/content/73/3/1128
*  Intratumoral HPV16-Specific T Cells Constitute a Type I-Oriented Tumor Microenvironment to Improve Survival in HPV16-Driven...
Vaccine-induced tumor necrosis factor-producing T cells synergize with cisplatin to promote tumor cell death. Clin Cancer Res ... A dense tumor infiltration by T cells (38) and DCs (39) and a predominant adaptive immune gene signature (4) have been ... 4A). Stimulation of the tumor cells with IFNγ and/or TNFα or culture supernatant from antigen-stimulated HPV-specific Th1 or ... Treatment of tumor cells.. Tumor cells were seeded (15,000-27,500 cells/well) in a flat-bottom, 96-well plate (Costar/Thermo ...
  http://clincancerres.aacrjournals.org/content/24/3/634
*  Endothelial progenitor cells in non-Hodgkin's lymphoma | Haematologica
In cancer, angiogenic factors can be produced by the tumor cells themselves but also by surrounding hematopoietic cells in the ... 2004) The involvement of endothelial progenitor cells in tumor angiogenesis. J Cell Mol Med 8:294-300. ... more closely resembled that of freshly isolated endothelial cells from tumors rather than that of cultured endothelial cells.11 ... the gene expression profile of EPC ... progenitor cells in human non-small cell lung cancer. Cancer ...
  http://www.haematologica.org/content/92/4/433
*  Abstract 3026: Targeting GPX4 in tumor-associated stromal cells increases inflammatory-cell infiltration | Cancer Research
Consistent with this, GPX4 inhibition of stroma-cancer co-cultures suppressed T-cell chemotaxis but combined inhibition of GPX4 ... findings that 15-LO gene amplifications are inversely correlated with T-cell infiltration in breast cancers in The Cancer ... RSL3 was selectively cytotoxic to stromal cells over cancer cells, in comparisons of immortalized cell lines as well as ... Up to 55% of triple-negative breast cancers have 'stroma-rich' tumors with markedly lower T-cell inflammation. Here we report a ...
  http://cancerres.aacrjournals.org/content/77/13_Supplement/3026
*  Antisense DNA Therapy | SpringerLink
Many cancers are due to overexpression of the genes that promote cell... ... Many cancers are due to overexpression of the genes that promote cell proliferation, called tumor suppressor genes. Antisense ... Antisense nucleic acids are widely used to study the effect of genes in cultured cells. The potential of antisense nucleic ... In: Schwab M. (eds) Encyclopedia of Cancer. Springer, Berlin, Heidelberg. * .RIS Papers ...
  https://link.springer.com/referenceworkentry/10.1007/978-3-642-27841-9_335-2