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Devising new lipid-coated calcium phosphate/carbonate hybrid nanoparticles for controlled release in endosomes for efficient...
... are proven to be effective vehicles for the delivery of genes and some drugs, while it is not desirable for NPs to release ... genes/drugs in late endosomes/lysosomes. To achieve the early endosomal release and escape, we have designed and developed new ... Lipid-coated calcium phosphate (LCP) nanoparticles (NPs) are proven to be effective vehicles for the delivery of genes and some ... To achieve the early endosomal release and escape, we have designed and developed new lipid-coated calcium carbonate/phosphate ...http://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb01635b
Growth factor gene expression profiles of bone morphogenetic protein-2-treated human adipose stem cells seeded on calcium ...
Subsequently, hASCs were seeded on plastic or on biphasic calcium phosphate (BCP) consisting of 60% hydroxyapatite and 40% β- ... Growth factor gene expression profiles of bone morphogenetic protein-2-treated human adipose stem cells seeded on calcium ... tricalcium phosphate. A PCR array for ∼150 trophic factors and differentiation-related genes was performed at day 21 of culture ... Expression of another ∼50 genes was decreased ≥2-fold on BCP vs. plastic, even though hASCs differentiate better on BCP than on ...http://dare.uva.nl/search?metis.record.id=403446
Protocols and Video Articles Authored by L. James Lee (Translated to Hindi)
Lipid-coated Nano-calcium-phosphate (LNCP) for Gene Delivery International Journal of Pharmaceutics. Jun, 2010 , Pubmed ID: ... Here we report a novel gene transfer vector comprising lipid-coated nano-calcium-phosphate (LNCP) that provides consistently ... It is based on core-shell nanoparticles comprising a calcium-phosphate core and a cationic lipid shell. This method, in ... The LNCP transfected pDNA 24 times greater than the naked pDNA and 10-fold greater relative to the standard calcium-phosphate ...https://www.jove.com/author/L.+James_Lee?language=Hindi
Stem and progenitor cells: advancing bone tissue engineering | SpringerLink
Effect of NELL1 gene overexpression in iPSC-MSCs seeded on calcium phosphate cement. Acta Biomater. 2014. doi: 10.1016/j.actbio ... Mol Ther J Am Soc Gene Ther. 2013;21(8):1631-8. doi: 10.1038/mt.2013.109.CrossRefGoogle Scholar ... Expression of VEGF gene isoforms in a rat segmental bone defect model treated with EPCs. J Orthop Trauma. 2012;26(12):689-92. ... Dual delivery of rhPDGF-BB and bone marrow mesenchymal stromal cells expressing the BMP2 gene enhance bone formation in a ...https://link.springer.com/article/10.1007%2Fs13346-015-0235-1
A New Binding Motif for the Transcriptional Repressor REST Uncovers Large Gene Networks Devoted to Neuronal Functions | Journal...
1998) Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in ... presumably duplicated genes extends to many other gene families as well, including those encoding calcium and potassium voltage ... 1995)] or green fluorescent protein (GFP) were introduced into MEFs by calcium phosphate precipitation (Fasbender et al., 1998 ... The RE1 site for the Gad1 gene, a gene previously shown to be repressed by REST (Lunyak et al., 2002), served as a control. As ...http://www.jneurosci.org/content/27/25/6729
Myocardial grafts and cellular compositions useful for same - FIELD LOREN J.
The MT-TGF fusion gene was introduced into C2C12 myoblasts by calcium phosphate transfection; stable transfectants were ... gene. The pGK-HYG plasmid provides selection for transfected ES cells, while the mMHC-neo. r gene facilitates a second round of ... Thus, a marker gene, such as a gene conferring antibiotic resistance (e.g. neomycin or hygromycin), is introduced into the stem ... 0085] A fusion gene comprised of the metallothionein (MT) promoter driving a modified Transforming Growth Factor-Beta 1 (TGF-β1 ...http://www.freepatentsonline.com/y2002/0061295.html
Diglons are heterodimeric proteins composed of IgLON subunits, and Diglon-CO inhibits neurite outgrowth from cerebellar granule...
Chen, C. A. and Okayama, H. (1988). Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably ... or from calcium-phosphate-mediated transient transfection of HEK 293 human embryonic kidney cells, using methods described ... Is the expression of the different gene products coordinated or can LAMP-OBCAM heterodimers exist alongside excess OBCAM? In ... 0.12 M sodium phosphate buffer (pH 7.4), 1% bovine serum albumin (BSA)] containing antisera (1:100) or IgLON-Fc (25 μg ml-1) ...http://jcs.biologists.org/content/117/17/3961
Simulated body fluid - Wikipedia
The in vitro studies showed higher gene delivery efficiency for the calcium-phosphate/DNA complexes made of SBF solution than ... An attempt was made to investigate the application of SBF in gene delivery. Calcium phosphate nanoparticles, required for the ... "Calcium phosphate-mediated gene delivery using simulated body fluid (SBF)". International Journal of Pharmaceutics. 434: 199- ... The consumption of calcium and phosphate ions, present in the SBF solution, results in the spontaneous growth of bone-like ...https://en.wikipedia.org/wiki/Simulated_body_fluid
1988) Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with ... was performed through calcium phosphate precipitation (24). After 2 days, the cells were harvested by being scraped into ... The cell pellet was washed twice in 10 mm potassium phosphate, pH 7.4, with pelleting between washes before being resuspended ... in assay buffer (10 mmpotassium phosphate, pH 7.4, 100 mm potassium chloride) and homogenization by passage through a 27-gauge ...http://molpharm.aspetjournals.org/content/52/5/874
1988) Calcium phosphate-mediated gene transfer: A highly efficient transfection system for stably transforming cells with ... Cells were transiently transfected with WT or mutant cDNAs using a modification of the calcium phosphate coprecipitation method ... 1994) Organisation of the mouse 5-HT3 receptor gene and the functional expression of two splice variants. Mol Brain Res 26:233- ... 1987) Kinetics and selectivity of a low-voltage-activated calcium current in chick and rat sensory neurones. J Physiol 386:547- ...http://molpharm.aspetjournals.org/content/57/6/1249
Patent US5460830 - Biochemically active agents for chemical catalysis and cell receptor activation - Google Patents
Therapeutic calcium phosphate particles for use in inhibiting expression of a gene. ... Therapeutic calcium phosphate particles and methods of manufacture and use. US20010048925 *. Feb 27, 2001. Dec 6, 2001. Bell ... Therapeutic calcium phosphate particles and methods of manufacture and use. US20020014182 *. Feb 20, 2001. Feb 7, 2002. Tapesh ... Therapeutic calcium phosphate particles and methods of making and using same. WO2017011292A1 *. Jul 8, 2016. Jan 19, 2017. ...http://www.google.com/patents/US5460830?dq=6,064,942
An Evaluation on Transfection Efficiency of pHRE-Egr1-EGFP in Hepatocellular Carcinoma Cells Bel-7402 Mediated by PEI-MZF-NPs
Y. Liu, T. Wang, F. He et al., "An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery," ... T. Shibata, A. J. Giaccia, and J. M. Brown, "Development of a hypoxia-responsive vector for tumor-specific gene therapy," Gene ... Q. S. Tang, D. S. Zhang, and N. Gu, "Synthesis and in vitro study of PEI-coated MneZn ferrite e a novel gene vector," Journal ... H. Akita and H. Harashima, "Nonviral gene delivery," Contributions to Nephrology, vol. 159, pp. 13-29, 2008. View at Publisher ...https://www.hindawi.com/journals/jnm/2011/136052/ref/
Hydroxyapatite: Design with Nature | SpringerLink
Hanifi A, Fathi MH, Sadeghi HM, Varshosaz J. Mg2+ substituted calcium phosphate nano particles synthesis for non viral gene ... Hu Q, Tan Z, Liu Y, Tao J, Cai Y, Zhang M, Pan H, Xu X, Tang R. Effect of crystallinity of calcium phosphate nanoparticles on ... Macroporous calcium phosphate ceramic: a prospective study of 106 cases in lumbar spinal fusion. J Long-Term Eff Med Implants. ... Lin X, de Groot K, Wang D, Hu Q, Wismeijer D, Liu Y. A review paper on biomimetic calcium phosphate coatings. Open Biomed Eng J ...https://link.springer.com/chapter/10.1007/978-3-319-73664-8_6
Frontiers | Live imaging of synaptic vesicle release and retrieval in dopaminergic neurons | Frontiers in Neural Circuits
We then optimized a strategy for transient transfection of spH into DA neurons, ultimately using calcium-phosphate gene ... James Rothman, Yale University) using calcium phosphate-mediated gene transfer. Experiments were typically conducted at 14-40 ... Balaji, J., Armbruster, M., and Ryan, T. A. (2008). Calcium control of endocytic capacity at a CNS synapse. J. Neurosci. 28, ... Finally these approaches should also be well suited for studying mutations in genes such as α-synuclein that are known to both ...https://www.frontiersin.org/articles/10.3389/neuro.04.003.2009/full
News Archive - NUI Galway
"Design and Functionalisation of Collagen/Calcium Phosphate Scaffolds for Non-Viral Gene Delivery in Bone Tissue Engineering". ... "Our work at NUI Galway shows that bacteria can change their genes and even swap genes with other bacteria very quickly. Some ... The more often we use antibiotics, the better the chance that a bacteria with a gene that breaks down the antibiotic will ... genes can give bacteria the power to smash the antibiotics into pieces before they have a chance to work. ...https://www.nuigalway.ie/about-us/news-and-events/news-archive/79/
Soft-tissue sarcomas were generated by intramuscular injection of a calcium phosphate precipitate of Ad-Cre (Gene Transfer ... which target more than 24,000 genes. Image analysis was conducted using Illumina's BeadStudio v3.0.14 Gene Expression Module. ... Gene Expression Analysis. RNA was isolated from human tumor tissue using the RNeasy Kit (Qiagen), and quality was analyzed ... A, left and middle, relative gene expression in human metastatic (N = 5) and nonmetastatic (N = 8) UPS and fibrosarcoma in ...http://cancerdiscovery.aacrjournals.org/content/3/10/1190
Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs
... gene delivery to HeLa cells demonstrated significant reporter gene expression compared to lipofection and calcium phosphate ... "Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection," Gene, vol. 270, no. 1-2, pp. 61- ... Reporter gene expression was observed 48 hr after transfection and the result showed that the reporter gene expression was ... Figure 6: BALB/c mice lung and trachea luciferase reporter gene expression analysis. (a) Gene delivery efficiency of various ...https://www.hindawi.com/journals/bmri/2014/646787/
Patent US7413753 - Calcium phosphate delivery vehicles for osteoinductive proteins - Google Patents
The composition comprises an osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of an ... The Frizzled family of genes and proteins is described in Wang et al., J. Biol. Chem. 271:4468-4476 (1996). The active agent ... the calcium phosphate material is a poorly crystalline apatitic calcium phosphate solid having a calcium-to-phosphate (Ca/P) ... Calcium Phosphate. In one embodiment, the calcium phosphate material is poorly crystalline apatitic (PCA) calcium phosphate. ...http://www.google.ca/patents/US7413753
Patent US20010020011 - Polymeric gene delivery system - Google Patentsuche
... with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a ... The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid ... where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve ... polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a ...http://www.google.de/patents/US20010020011?hl=de
Patente US9295756 - Methods for inducing immune tolerance to organ transplants - Google Patentes
In a specific embodiment, a calcium-phosphate precipitate containing DNA encoding the gene(s) of interest can be prepared using ... neomycin resistant gene, ampicillin resistance gene, E. coli gpt gene or the like. ... Since the gene transferred by the retroviral vector is integrated into chromosomal DNA of the host stem cell, the gene is ... Twenty-four hours after plating the cells, the calcium phosphate precipitate containing approximately 20 μg/ml of the ...http://www.google.es/patents/US9295756
Patente US7169904 - Immunocytokine sequences and uses thereof - Google Patentes
... including via calcium phosphate, a "gene gun", adenovirus vectors, cationic liposomes, retroviral vectors, or any other ... Treatment of Cancer by Gene Therapy. The nucleic acids of the invention can be used as gene therapy agents for treatment of ... 7:798-804). Alternative methods include electroporation or calcium phosphate precipitation. Other useful methods of producing ... washed with 10 mM sodium phosphate pH 7; and eluted with 100 mM sodium phosphate, 150 mM NaCl pH 3.5. The pH of the eluted ...http://www.google.es/patents/US7169904?dq=flatulence
Patente US20070128245 - Porous calcium phosphate bone material - Google Patentes
... the calcium phosphate compositions are remodeled into bone. Methods for using the calcium phosphate compositions, e.g., to ... In addition to calcium phosphate, the compositions include an effervescent agent to promote the formation of interconnected ... Porous calcium phosphate implant compositions that approximate the chemical composition of natural bone mineral are provided. ... genes, gene fragments, gene regulatory sequences, and antisense molecules), nucleoproteins, polysaccharides, glycoproteins, and ...http://www.google.es/patents/US20070128245
CD4+ T Cell-Derived IL-21 and Deprivation of CD40 Signaling Favor the In Vivo Development of Granzyme B-Expressing Regulatory B...
... followed by an internal ribosomal entry site and the eGFP gene, using the calcium phosphate method. The respective Δnef ... The NEMO patient was diagnosed with a hemizygous mutation in exon 3 of the NEMO gene (c.337G , A p.Asp113Asn) at Ulm University ... Generation of wild-type HIV-1 (NL4-3-based) proviral constructs containing a functional nef gene, followed by an internal ... These cells were maintained in DMEM and cotransfected with an NL4-3 proviral construct carrying a functional nef gene, ...http://www.jimmunol.org/content/194/8/3768
Webinar - Transfection Technologies Market (2012-2017) | MarketsandMarkets
Calcium Phosphate, Electroporation, Nucleofection, Magnetofection, Gene Gun, Viral) And Types (Gene Delivery, DNA Delivery, ... Calcium Phosphate, Electroporation, Nucleofection, Magnetofection, Gene Gun, Viral) And Types (Gene Delivery, DNA Delivery, ...https://www.marketsandmarkets.com/Webinar.asp?id=895
Docking interactions of the JNK scaffold protein WDR62 | Biochemical Journal
2001) Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection. Gene 270:61-68. ... HEK-293T cells were transfected with the appropriate expression plasmid using the calcium phosphate method . The total ... 1999) The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases. Mol. Cell. Biol. 19:1569-1581. ... Patients that harbour this mutation display a phenotype as severe as patients with upstream disruption of the WDR62 gene . ...http://www.biochemj.org/content/439/3/381