JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Longitudinal Sequence and Functional Evolution within Glycoprotein E2 in Hepatitis C Virus Genotype 3a Infection. ... Immunology, Issue 99, Antigen Capsid-Incorporation strategy, transgene method, Adenovirus (Ad), vaccine, capsid proteins, dual ... and Proteins, De novo protein and peptide design, Drug design, In silico sequence selection, Optimization, Fold specificity, ... Protein-protein interaction, High-throughput screening, Luminescence, Yeast two-hybrid, HT-GPCA, Network, protein, yeast, cell ...https://www.jove.com/visualize/abstract/25970466/longitudinal-sequence-functional-evolution-within-glycoprotein-e2
WWP1 - Wikipedia
"Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... "Physical and functional interactions between the transactivation domain of the hematopoietic transcription factor NF-E2 and WW ... "Adenovirus protein involved in virus internalization recruits ubiquitin-protein ligases". Biochemistry. 41 (48): 14299-305. doi ... The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key ...https://en.wikipedia.org/wiki/WWP1
Method for treating a metastatic carcinoma using a conditionally lethal gene - Patent # 5997859 - PatentGenius
... represent the only adenovirus sequences required for cellular transformation. Berkner at 616. The E2 region encodes proteins ... "Functional Mapping of the Human Papillomavirus Type 11 Transcriptional Enhancer and its Interaction with the trans-acting E2 ... SEQUENCE LISTING - (1) GENERAL INFORMATION: - (iii) NUMBER OF SEQUENCES: 8 - (2) INFORMATION FOR SEQ ID NO:1: - (i) SEQUENCE ... More specifically, proteins expressed from viral vectors based on DNA viruses, such as adenovirus, simian virus 40, ...http://www.patentgenius.com/patent/5997859.html
Compositions and methods for the diagnosis and treatment of sepsis - The Regents of the University of Michigan
The deletion in the E1 region preferably extends from nucleotides 455 to 3329 in the sequence of the Ad5 adenovirus (PvuII- ... on the protein; in other words the antibody is recognizing and binding to a specific protein structure rather than to proteins ... To ensure the functional integrity of C5aRa after the labeling procedure, chemotaxis assays were performed using 125I-C5aRa (10 ... the E2 region (e.g., WO94/28938), the E4 region (e.g., WO94/28152, WO94/12649 and WO95/02697), or in any of the late genes L1- ...http://www.freepatentsonline.com/7455837.html
Abstracts from the British Association for the Study of the Liver Meeting Held on 13-14 September 2001 at the Royal Free...
Despite the deletion of the hydrophobic region of the HCV E2 sequence protein secretion was not observed. Detectable levels of ... lntratumoral coinjection of two adenoviruses, one encoding the chemokine IFN-gamma-inducible protein-lO and another encoding IL ... 67 FUNCTIONAL INTERPLAY OF FACTORS BINDING TO THE UTE-1 AND SERUM RESPONSE ELEMENT (SRE) OF THE TIMP-1 PROMOTER DIRECTS HIGH- ... Antibody responses to E2 and FrC were assessed by ELISA.. Results: In vitro expression of FrC-E2 and the E2 construct was ...http://gut.bmj.com/content/50/5/e1
Patent US5856152 - Hybrid adenovirus-AAV vector and methods of use therefor - Google Patents
ITR sequences from an AAV, and a selected transgene. Other hybrid vectors form a polycation conjugate and incorporate an AAV ... The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ... a human kidney cell line containing a functional adenovirus E1a gene which provides a transacting E1a protein). Other ... E2, E3, and E4) followed by late genes (e.g. L1-5), first generation adenovirus vectors for gene therapy focused on the removal ...http://www.google.com/patents/US5856152?dq=7143430
Acetylation of Nrf2 by p300/CBP Augments Promoter-Specific DNA Binding of Nrf2 during the Antioxidant Response
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated acetylation. J. Biol. Chem. 27610715-10721. [PubMed] ... The proteins were resolved on SDS-PAGE and subjected to immunoblotting with anti-HA antibodies to detect Nrf2. The sequences of ... p300 was cloned based on its interaction with the adenovirus-transforming protein E1A, while CBP was identified by its ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2682049/
ARTICLES | Physiology
BCL2/adenovirus E1B 19-kDa-interacting protein; NIX, NIP3-like; P4HA1, prolyl-4-hydroxylase α1-subunit; PFKFB3, 6-phosphofructo ... and RBX1 to form an E3 ubiquitin-protein ligase capable of functioning with E1 ubiquitin-activating and E2 ubiquitin- ... Coactivators interact with both sequence-specific DNA binding proteins such as HIF-1 and with the general transcription factors ... Erythropoietin reduces myocardial infarction and left ventricular functional decline after coronary artery ligation in rats. ...http://physiologyonline.physiology.org/content/19/4/176?ijkey=86490f33858df8b0d2ecef474e9b92e8a3eca099&keytype2=tf_ipsecsha
Chromatin associated proteins of Ustilago maydis, Seema Dutta. High-throughput DNA sequence analysis for the investigation of ... Structural and functional analysis of the two human tumor necrosis factor receptors, Paul Chih-Hsueh Chen ... Characterization of the transcriptional regulatory functions of the transforming region of the adenovirus E1A oncoprotein, ... Pathways of lipopolysaccharide-induced prostaglandin E2 production in human microvascular endothelial cells, Kimberly Anne ...http://jdc.jefferson.edu/theses/index.3.html
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11202790:Structural and Functional Analysis of the cis-Acti. *11202791:Interaction of Bovine Papillomavirus E2 Protein wi ... 11202506:Adenovirus-Mediated RNA Interference against Foot-. *11202507:Plasmid DNA Sequences Present in Conventional Herp ...http://www.100md.com/index/other/byinfono/022/406.htm
Cell proliferation, tumorogenicity, and apoptosis sub-cluster 38
F13 is a neutralization escape variant selected with M1-1A and contains amino acid mutations N83Y and M186I in the HN protein, ... RUBV virions contain two cysteine-rich glycoproteins, E2 and E1. The latter is believed to be involved in the membrane fusion. ... To facilitate communication, functional differences are usually required between interacting cells, which can be established ... Glycoprotein B (gB) homologues within the herpesvirus family display high sequence conservation, and a number of gB homologues ...http://www.biomedsearch.com/cluster/2/Cell-proliferation-tumorogenicity-and-apoptosis/sub-38-p3.html
Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in...
"Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in ... Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine ... Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer's patches ... Dongjin to express a recombinant E2 protein (rE2-TRCs). The expression of the recombinant E2 protein (rE2) in rE2-TRCs was ...https://www.deepdyve.com/lp/springer_journal/induction-of-immune-responses-in-mice-and-pigs-by-oral-administration-oVRvTfh06U
Search: All Pfam signatures | InterPro | EMBL-EBI
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Zein seed storage protein (IPR002530) Pfam signature: PF01559 Hepatitis C virus non-structural protein E2/NS1 (IPR002531) Pfam ... Adenovirus Pll, hexon, N-terminal (IPR016107) Pfam signature: PF01065 CDP-alcohol phosphatidyltransferase (IPR000462) Pfam ...http://www.ebi.ac.uk/interpro/member-database/Pfam
Nonsense Mutations in hERG Cause a Decrease in Mutant mRNA Transcripts by Nonsense-Mediated mRNA Decay in Human Long-QT...
The mRNA from uninfected myocytes was used as control (CON). The level of E2 transcripts (E2) from adenovirus served as a ... Several LQT2 nonsense and frameshift mutations have been studied at the functional and protein levels with cDNA constructs.8,12 ... an FRT site sequence is integrated into the genome of HEK293 cells and recombined by Flp recombinase with the FRT site of the ... The expression level of the hygromycin resistance gene from the pcDNA5/FRT vector or the E2 gene from adenovirus was used as a ...http://circ.ahajournals.org/content/116/1/17
US6764709B2 - Method for making and measuring a coating on the surface of a medical device using an ultraviolet laser ...
... nanoparticles and microparticles with and without targeting sequences such as the protein transduction domain (PTD). The ... Viral vectors include adenoviruses, gutted adenoviruses, adeno-associated virus, retroviruses, alpha virus (Semliki Forest, ... angiogenic substances, such as acidic and basic fibrobrast growth factors, estrogen including estradiol (E2), estriol (E3) and ... creating functional groups on the surface by RF plasma with water vapor, and then applying the polysaccharide using ...https://patents.google.com/patent/US6764709
Adenovirus DNA Replication | SpringerLink
... the adenoviruses have proved to be useful models for probing the basic mechanisms of macromolecular synthesis in eukaryotic ... DNA sequence analysis of the region encoding the terminal protein and the hypothetical N-gene product of adenovirus type 2, J. ... Purification of the terminal protein in a functional form, Proc. Natl. Acad. Sci. U.S.A. 78:6779.PubMedGoogle Scholar ... Further studies of early polypeptides in vivo and localization of E2 and E2A to the cell plasma membrane, Virology 7:518.Google ...https://link.springer.com/chapter/10.1007/978-1-4684-7935-5_7
US20030012770A1 - Recombinant p53 adenovirus methods and compositions - Google Patents
Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 ... Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and ... Virus lacking functional E2 and E2-expressing cells may be used, as may virus lacking functional E4 and E4-expressing cells, ... The large E1B protein together with the E4orf6 protein target p53 for active degradation in adenovirus infected cells. ...https://patents.google.com/patent/US20030012770A1/en
METHOD OF SUPPRESSING GENE TRANSCRIPTION THROUGH HISTONE LYSINE METHYLATION - Patent application
Viral proteins such as human adenovirus-5 E1A protein and bovine herpesvirus-1 VP8 protein contain basic KRPR and RRPR patches ... sequences. For example, operative linkage of a nucleic acid to a promoter refers to the physical and functional relationship ... For instance, the papillomavirus E2 protein binds Brd4 to tether the viral genome to mitotic chromosomes to ensure persistence ... adenovirus E1A protein interacts with the retinoblastoma protein p130 to disrupt a network of protein interactions required for ...http://www.patentsencyclopedia.com/app/20160137989
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Phenotypes are determined by a complex series of physical (e.g. protein-protein) and functional (e.g. gene-gene or genetic) ... Genetics, Issue 72, Molecular Biology, Immunology, Medicine, Genomics, Proteins, RNA-seq, Next Generation DNA Sequencing, ... They include ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, and ubiquitin ligase E3. Unlike to E1 and E2, E3 ... Cellular Biology, Issue 13, Springer Protocols, hair follicles, liposomes, adenovirus, genes, stem cells ...https://www.jove.com/visualize/abstract/25158067/genome-wide-transcriptional-profiles-berry-skin-two-red-grape
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Briefly, the adenovirus has the sequence for a red fluorescent protein (Ds Red DR from Clontech) under the control of a hypoxia ... They include ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, and ubiquitin ligase E3. Unlike to E1 and E2, E3 ... As an alternative treatment option to exogenous insulin injection, transplantation of functional pancreatic tissue has been ... Significant changes are confirmed with targeted qPCR and then protein detection. We probe for tissue-based cytokine protein ...https://www.jove.com/visualize/abstract/22506063/microtubular-stability-affects-pvhl-mediated-regulation-hif-1alpha
WWP2 - NEDD4-like E3 ubiquitin-protein ligase WWP2 - Homo sapiens (Human) - WWP2 gene & protein
... the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation ... E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ... between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.,p>, ... Integrated resource of protein families, domains and functional sites. More...InterProi. View protein in InterPro. IPR000008 C2 ...https://www.uniprot.org/uniprot/O00308
Protocols and Video Articles Authored by Hong He (Translated to Hindi)
The 4622-base pair cDNA encodes a protein of 1473 AA with >70% sequence identity and all typical domains of other A2M-family ... mediated the effect of E2 on CF growth by the gain of function analysis using an adenovirus vector. One hundred nanomoles per ... Quantitative and Functional Changes of T Helper Cell Subsets in the Bone Marrow of Severe Aplastic Anemia Patients] Zhonghua ... Proteins. Dec, 2007 , Pubmed ID: 17803223 Protein-protein docking is usually exploited with a two-step strategy, i.e., ...https://www.jove.com/author/Hong_He?language=Hindi
Professor Ellie (Eleanor) Barnes - Nuffield Department of Medicine
In parallel, the core region and E2 protein were sequenced. Gamma interferon-secreting CD4+ T-cell responses directed against ... human adenovirus 6 (Ad6) and chimpanzee adenovirus 3 (ChAd3)]. Both vectors primed T cell responses against HCV proteins; these ... Further defining the functional significance of these regions may have important implications for genotype 3a E2 virus-receptor ... To identify novel vaccine carriers suitable for vaccine delivery in humans, we isolated and sequenced more than 1000 adenovirus ...https://www.ndm.ox.ac.uk/principal-investigators/researcher/ellie-eleanor-barnes
Mutation site and context dependent effects of ESR1 mutation in genome-edited breast cancer cell models | Breast Cancer...
RNA sequencing (RNA-seq) was employed to study gene targets of mutant ER. Cells with ESR1 mutations displayed ligand- ... However, their functional role is not well-understood, at least in part due to a lack of ESR1 mutant models. Here, we describe ... or 1 nM E2 plus 1 μM of Ful for 24 h. RNA was isolated, and GREB1 or insulin-like growth factor-binding protein 4 (IGFBP4) ... However, their functional role is not well-understood, at least in part due to a lack of ESR1 mutant models. Here, we describe ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-017-0851-4
De novo derivation of proteomes from transcriptomes for transcript and protein identification.
Here we demonstrate that, for a non-model species, the sequencing of expressed mRNA can generate a protein d ... Identification of proteins by tandem mass spectrometry requires a reference protein database, but these are only available for ... Thus, at 8 hours post infection most reads map to adenovirus early genes (E1, E2, E3 and E4) whereas by 24 hours most reads map ... Indeed, de novo assembly from raw sequence data has clear benefits for gene identification and the functional annotation of ...http://www.biomedsearch.com/nih/De-novo-derivation-proteomes-from/23142869.html