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*  Ultrastructural Organization of Bovine Chromaffin Cell Cortex-Analysis by Cryofixation and Morphometry of Aspects Pertinent to...
After cold metal freezing, freeze substitution in (UO2)2+ acetate in methanol and methacrylate embedding 326 nm were reported ( ... Frozen sandwich samples were split under liquid N2 or in the freeze-substitution medium (see below) at −80°C. Both fracture ... 1965) A study of extracellular space in central nervous tissue by freeze-substitution. J Cell Biol 25:117-137, pmid:14283623.. ... 2 and 3 show examples of chromaffin cells after the cryofixation/freeze substitution was applied. DVs identified by their ...
  http://jcb.rupress.org/content/139/7/1709
*  Mesosome | Open Access articles | Open Access journals | Conference Proceedings | Editors | Authors | Reviewers | scientific...
... with advances in cryofixation and freeze substitution methods for electron microscopy, it was generally concluded that ... "Electron microscopy of frozen-hydrated bacteria". J. Bacteriol. 155 (1): 381-90. PMC 217690. PMID 6408064 ... Mesosomes form in bacterial cells prepared for electron microscopy by chemical fixation, but not by freeze-fracture fixation.[1 ...
  http://research.omicsgroup.org/index.php/Mesosome
*  EMS Freeze Substitution Kit
Freeze substitution is a process for low temperature dehydration and fixation of rapidly frozen cells that usually takes days ... Freeze Substitution is a process for low temperature dehydration and fixation of rapidly frozen cells that usually takes days ... EMS Freeze Substitution Kit with Shaker. kit. 1,595.00. Add to Cart. 34500-S. EMS Freeze Substitution Kit without Shaker. kit. ... EMS Freeze Substitution Kit. EMS Freeze Substitution Kit. Overview. ...
  https://www.emsdiasum.com/microscopy/products/cryofixation/freeze_kit.aspx
*  Quantitative X-ray microanalysis of calcium in sea urchin eggs after quick-freezing and freeze-substitution | SpringerLink
Freeze-substitution was used to study the distribution of calcium in sea urchin eggs, and the validity of the technique was ... Quantitative X-ray microanalysis of calcium in sea urchin eggs after quick-freezing and freeze-substitution. Validity of the ... Ornberg RL, Reese TS (1981) Quick freezing and freeze substitution for X-ray microanalysis of calcium. In: Hutchinson T, Somlyo ... Freeze-substitution was used to study the distribution of calcium in sea urchin eggs, and the validity of the technique was ...
  https://link.springer.com/article/10.1007/BF00524764
*  Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze...
We imaged in vitro GCX in response to TNF-α using rapid freezing/freeze substitution (RF/FS) transmission electron microscopy ( ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ...
  http://atvb.ahajournals.org/content/35/Suppl_1/A450
*  Freeze substitution reveals a new model for sporangial cleavage in Phytophthora, a result with implications for cytokinesis in...
Rapid freezing and freeze substitution (RF-FS) have been used to re-examine the process by which the multinucleate sporangium ... Rapid freezing and freeze substitution (RF-FS) have been used to re-examine the process by which the multinucleate sporangium ... Freeze substitution reveals a new model for sporangial cleavage in Phytophthora, a result with implications for cytokinesis in ... article{Hyde1991FreezeSR, title={Freeze substitution reveals a new model for sporangial cleavage in Phytophthora, a result with ...
  https://www.semanticscholar.org/paper/Freeze-substitution-reveals-a-new-model-for-in-a-in-Hyde-Lancelle/6cc922e33c40d379fc157ef7f654c4384939244d
*  Postnatal development and control of the pulmonary surfactant system in the tammar wallaby Macropus eugenii | Journal of...
Freeze substitution and immunohistochemistry. The methods used for freeze substitution of lung tissue were similar to those ... The tissue was then cryofixed by plunge-freezing in liquid nitrogen. Dehydration and embedding were performed in an Automatic ... Preservation and immunogold localization of lipids by freeze-substitution and low temperature embedding. Scan. Microsc. (Suppl ... freeze-substitution system (Leica Reichert, Austria). Dehydration of the tissue was achieved with methanol containing 0.5 % ...
  http://jeb.biologists.org/content/204/23/4031.full
*  A conserved family of proteins facilitates nascent lipid droplet budding from the ER | JCB
Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution ... HPF and automatic freeze substitution. HPF and automatic freeze substitution was performed as previously described (Walther and ... The automatic freeze substitution unit was programmed to keep samples frozen at −90°C for 22 h. The temperature was increased ... The cells were then subjected to high-pressure freezing (HPF), automatic freeze substitution, and visualized by EM and EM ...
  http://jcb.rupress.org/content/211/2/261
*  Electron microscopy (EM) core facility | Biocenter Oulu
Freezing is followed by freeze substitution, which is done at temperature below -70°C, where water (in form of ice) in sample ... High-pressure freezing and freeze substitution (HPF-FS). One of the best available methods of biological sample preparation for ... EM-Pact high pressure freezing device (Leica). *Leica EM AFS2 freeze-substitution device with automatic liquid handling system ... followed by freeze-substitution (FS). This method enables the observation of cells and cellular organelles in close-to-native ...
  http://www.oulu.fi/biocenter/em
*  Plus it
High-Pressure Freezing-Freeze Substitution and Transmission Electron Microscopy. Gametophytes of P. patens GD06 and PpCESAKOs ... 4). When we attempted to prepare specimens by high-pressure freezing and freeze substitution, the leaves fractured in a plane ... were high-pressure frozen using a Leica EMPACT2 high-pressure freezer (Leica Microsystems) followed by freeze substitution in ... CBM3a affinity cytochemistry and freeze substitution transmission electron microscopy were supported by the Australian Research ...
  http://www.plantphysiol.org/content/175/1/210
*  Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture...
Freeze substitution and Lowicryl embedding.. Male SD rats (9-14 weeks old) were anesthetized with sodium pentobarbital and ... The sections were then frozen by a high-pressure freezing machine (HPM 010; Bal-Tec, Balzers, Liechtenstein) and fractured by ... in a freeze substitution system (EM AFS; Leica). The temperature was raised stepwise to −45°C. The samples were infiltrated ... 1995) Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane ...
  http://www.jneurosci.org/content/27/8/2135
*  'osmium tetroxide' Protocols and Video...
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy', 'Scanning ... Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy', 'A Magnetic Microbead Occlusion ... Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron ... Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy. Krzysztof ...
  https://www.jove.com/keyword/osmium+tetroxide
*  'epoxy resins' Protocols and Video...
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy', 'A Novel ... Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy. Krzysztof ...
  https://www.jove.com/keyword/epoxy+resins
*  Intracerebral Injections and Ultrastructural Analysis of High‐Pressure Frozen Brain Tissue - Current Protocols
Freezesubstitution protocols for improved visualization of membranes in high‐pressure frozen samples. J. Microsc. 212:53‐61. ... Improved preservation of ultrastructure in difficult‐to‐fix organisms by high pressure freezing and freeze substitution: I. ... Ultrastructure of growing epiphyseal cartilage processed by rapid freezing and freezesubstitution. J. Electron. Microsc. 32: ... Freezesubstitution and freeze‐drying. In Cryotechniques in Biological Electron Microscopy (R.A. Steinbrecht and K. Zierold, ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ns0227.html?quicktabs_cp=materials
*  Association with Soil Bacteria Enhances p38-Dependent Infection Resistance in Caenorhabditis elegans | Infection and Immunity
Freeze substitution in 3 hours or less. J. Microsc. 243:227-233. ... Recent advances in high-pressure freezing: equipment- and ... and fixated by freeze substitution using a 1% OsO4 and 0.1% uranyl acetate solution (26, 27). Following embedding in Epon resin ...
  https://iai.asm.org/content/81/2/514?ijkey=85f296e5090b5df5c8247f09a1fcc1e097bd2444&keytype2=tf_ipsecsha
*  MPI CBG Research Group -: Services
Leica Automatic Freeze Substitution units (AFS and AFS2). *Leica EM GP grid plunger ... Sample preparation for cryo-immobilization (High-pressure freezing, Plunge freezing), or chemical fixation, including microwave ...
  https://www.mpi-cbg.de/services-facilities/core-facilities/electron-microscopy/services/
*  Brief communication State of preservation of tissues from ancient human remains found in a glacier in Canada.
Processed by freeze substitution. (B) Lysosome (ly) and lipid drops (l). Processed by freeze substitution. (C) Profiles of rough ... Other frozen lung and heart tissues were freeze substituted through acetone and osmium for transmission electron microscopy. ... Processed by freeze substitution. (D) Fibrillar collagen from heart. Processed by freeze substitution. the characteristic axial ... For the tissues processed by freeze substitution, we used a Leica Automatic Freeze Substitution unit. First, tissues were fixed ...
  https://www.docme.ru/doc/1913279/brief-communication--state-of-preservation-of-tissues-fro..
*  High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles | SpringerLink
Until recently, cellular EM was synonymous with rapid-freezing and freeze-substitution fixation (RF-FSF) protocols followed by ... Improved preservation of ultrastructure in difficult-to-fix organisms by high pressure freezing and freeze substitution: I. ... vitrified sectioning omit the freeze-substitution and plastic-embedding step and cut sections straight from a block of a frozen ... a, b 70 nm, frozen-hydrated cryo-section observed in a high-pressure frozen A31-3 T3 cell. a Approximately 10-nm thick ...
  https://link.springer.com/article/10.1007%2Fs00709-013-0600-1
*  A comparative morphology of the male genitalia of Aphididae (Insecta, Hemiptera): part 2.
22394188 - High-pressure freezing/freeze substitution and transmission electron microscopy for cha.... 25348328 - Dissociative ...
  http://www.biomedsearch.com/nih/comparative-morphology-male-genitalia-Aphididae/23144528.html
*  Glycocalyx - Wikipedia
"Imaging the Endothelial Glycocalyx In Vitro By Rapid Freezing/Freeze Substitution Transmission Electron Microscopy". ...
  https://en.wikipedia.org/wiki/Glycocalyx
*  Mesosome - Wikipedia
By the mid to late 1980s, with advances in cryofixation and freeze substitution methods for electron microscopy, it was ... Dubochet J, McDowall AW, Menge B, Schmid EN, Lickfeld KG (1 July 1983). "Electron microscopy of frozen-hydrated bacteria". J. ...
  https://en.wikipedia.org/wiki/Mesosome
*  Scintillons - Wikipedia
Fast-freeze Fixation/Freeze Substitution) contain a large number of electron dense bodies around the cell periphery. These ... Cells observed under the electron microscope after a technique involving rapid freezing of the cells followed by substitution ... binding protein and luciferase to the scintillons ofGonyaulax polyedra revealed by double immunolabeling after fast-freeze ...
  https://en.wikipedia.org/wiki/Scintillons
*  Operation Big Bird - Wikipedia
The Swiss Federal Office of Police then issued a freeze order in substitution for the exceptional freezing order of the Federal ... 2, freezing all Marcos assets in the Philippines. March 15, 1986 De Guzman and Dagher return to Vienna, and was able to get the ... Scheller replied by telex from Credit Suisse, but informs De Guzman that the freeze order is still in place. De Guzman finally ... They were initially informed by Scheller that the Swiss authorities have put a "freeze order" on all the accounts linked to the ...
  https://en.wikipedia.org/wiki/Operation_Big_Bird
*  List of MeSH codes (E05) - Wikipedia
... freeze drying MeSH E05.200.500.620.760.160.260.270 --- freeze substitution MeSH E05.200.500.620.760.720 --- tissue fixation ... frozen sections MeSH E05.200.500.620.620 --- replica techniques MeSH E05.200.500.620.620.150 --- corrosion casting MeSH E05.200 ... freeze drying MeSH E05.200.750.600.760.160.260.270 --- freeze substitution MeSH E05.200.750.600.760.720 --- tissue fixation ... freeze drying MeSH E05.760.156.260.270 --- freeze substitution MeSH E05.760.833.230 --- blood preservation MeSH E05.760.833.445 ...
  https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)