Quantitative X-ray microanalysis of calcium in sea urchin eggs after quick-freezing and freeze-substitution | SpringerLink
Freeze-substitution was used to study the distribution of calcium in sea urchin eggs, and the validity of the technique was ... Quantitative X-ray microanalysis of calcium in sea urchin eggs after quick-freezing and freeze-substitution. Validity of the ... Ornberg RL, Reese TS (1981) Quick freezing and freeze substitution for X-ray microanalysis of calcium. In: Hutchinson T, Somlyo ... Freeze-substitution was used to study the distribution of calcium in sea urchin eggs, and the validity of the technique was ...https://link.springer.com/article/10.1007/BF00524764
Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze...
We imaged in vitro GCX in response to TNF-α using rapid freezing/freeze substitution (RF/FS) transmission electron microscopy ( ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ... Abstract 450: Tumor Necrosis Factor Disrupts Endothelial Surface Glycocalyx Imaged by High-pressure Freezing, Freeze ...http://atvb.ahajournals.org/content/35/Suppl_1/A450
Postnatal development and control of the pulmonary surfactant system in the tammar wallaby Macropus eugenii | Journal of...
Freeze substitution and immunohistochemistry. The methods used for freeze substitution of lung tissue were similar to those ... The tissue was then cryofixed by plunge-freezing in liquid nitrogen. Dehydration and embedding were performed in an Automatic ... Preservation and immunogold localization of lipids by freeze-substitution and low temperature embedding. Scan. Microsc. (Suppl ... freeze-substitution system (Leica Reichert, Austria). Dehydration of the tissue was achieved with methanol containing 0.5 % ...http://jeb.biologists.org/content/204/23/4031.full
A conserved family of proteins facilitates nascent lipid droplet budding from the ER | JCB
Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution ... HPF and automatic freeze substitution. HPF and automatic freeze substitution was performed as previously described (Walther and ... The automatic freeze substitution unit was programmed to keep samples frozen at −90°C for 22 h. The temperature was increased ... The cells were then subjected to high-pressure freezing (HPF), automatic freeze substitution, and visualized by EM and EM ...http://jcb.rupress.org/content/211/2/261
Electron microscopy (EM) core facility | Biocenter Oulu
Freezing is followed by freeze substitution, which is done at temperature below -70°C, where water (in form of ice) in sample ... High-pressure freezing and freeze substitution (HPF-FS). One of the best available methods of biological sample preparation for ... EM-Pact high pressure freezing device (Leica). *Leica EM AFS2 freeze-substitution device with automatic liquid handling system ... followed by freeze-substitution (FS). This method enables the observation of cells and cellular organelles in close-to-native ...http://www.oulu.fi/biocenter/em
High-Pressure Freezing-Freeze Substitution and Transmission Electron Microscopy. Gametophytes of P. patens GD06 and PpCESAKOs ... 4). When we attempted to prepare specimens by high-pressure freezing and freeze substitution, the leaves fractured in a plane ... were high-pressure frozen using a Leica EMPACT2 high-pressure freezer (Leica Microsystems) followed by freeze substitution in ... CBM3a affinity cytochemistry and freeze substitution transmission electron microscopy were supported by the Australian Research ...http://www.plantphysiol.org/content/175/1/210
Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture...
Freeze substitution and Lowicryl embedding.. Male SD rats (9-14 weeks old) were anesthetized with sodium pentobarbital and ... The sections were then frozen by a high-pressure freezing machine (HPM 010; Bal-Tec, Balzers, Liechtenstein) and fractured by ... in a freeze substitution system (EM AFS; Leica). The temperature was raised stepwise to −45°C. The samples were infiltrated ... 1995) Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane ...http://www.jneurosci.org/content/27/8/2135
'osmium tetroxide' Protocols and Video...
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy', 'Scanning ... Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy', 'A Magnetic Microbead Occlusion ... Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron ... Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy. Krzysztof ...https://www.jove.com/keyword/osmium+tetroxide
'epoxy resins' Protocols and Video...
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy', 'A Novel ... Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy. Krzysztof ...https://www.jove.com/keyword/epoxy+resins
Intracerebral Injections and Ultrastructural Analysis of High‐Pressure Frozen Brain Tissue - Current Protocols
Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples. J. Microsc. 212:53‐61. ... Improved preservation of ultrastructure in difficult‐to‐fix organisms by high pressure freezing and freeze substitution: I. ... Ultrastructure of growing epiphyseal cartilage processed by rapid freezing and freeze‐substitution. J. Electron. Microsc. 32: ... Freeze‐substitution and freeze‐drying. In Cryotechniques in Biological Electron Microscopy (R.A. Steinbrecht and K. Zierold, ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ns0227.html?quicktabs_cp=materials
Brief communication State of preservation of tissues from ancient human remains found in a glacier in Canada.
Processed by freeze substitution. (B) Lysosome (ly) and lipid drops (l). Processed by freeze substitution. (C) Proﬁles of rough ... Other frozen lung and heart tissues were freeze substituted through acetone and osmium for transmission electron microscopy. ... Processed by freeze substitution. (D) Fibrillar collagen from heart. Processed by freeze substitution. the characteristic axial ... For the tissues processed by freeze substitution, we used a Leica Automatic Freeze Substitution unit. First, tissues were ﬁxed ...https://www.docme.ru/doc/1913279/brief-communication--state-of-preservation-of-tissues-fro..
A comparative morphology of the male genitalia of Aphididae (Insecta, Hemiptera): part 2.
22394188 - High-pressure freezing/freeze substitution and transmission electron microscopy for cha.... 25348328 - Dissociative ...http://www.biomedsearch.com/nih/comparative-morphology-male-genitalia-Aphididae/23144528.html
World-Wide Web Virtual Library: Microscopy
Bal-Tec - coating systems, high pressure and jet freezing, freeze fracturing, cryo transfer, freeze substitution, ion milling * ...http://www.ou.edu/research/electron/www-vl/long.shtml
Hydrotropism Interacts with Gravitropism by Degrading Amyloplasts in Seedling Roots of Arabidopsis and Radish | Plant Physiology
Robards AW, Sleytr UB (1985) Freeze-substitution and low temperature embedding. In AM Glauert, ed, Practical Methods in ... The root tips excised from the radish seedlings were frozen in absolute acetone containing 0.4% (v/v) glutaraldehyde in a vial ... Amyloplasts and columella cells were observed with low-vacuum SEM (JSM-5800LV, JEOL Ltd., Tokyo). A freeze substitution method ...http://www.plantphysiol.org/content/132/2/805?cited-by=yes&legid=plantphysiol
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
High-pressure freezing and freeze substitution are currently the methods of choice for producing the highest quality morphology ... a process called freeze substitution. Freeze substitution is typically carried out over several days in dedicated, costly ... Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy ... Here we present a protocol combining high-pressure freezing and quick freeze substitution that enables plant sample fixation to ...https://www.jove.com/visualize/abstract/23734222/increase-il-12-following-reconstruction-for-total-en-bloc
Ultrastructural Organization of Bovine Chromaffin Cell Cortex-Analysis by Cryofixation and Morphometry of Aspects Pertinent to...
After cold metal freezing, freeze substitution in (UO2)2+ acetate in methanol and methacrylate embedding 326 nm were reported ( ... Frozen sandwich samples were split under liquid N2 or in the freeze-substitution medium (see below) at −80°C. Both fracture ... 1965) A study of extracellular space in central nervous tissue by freeze-substitution. J Cell Biol 25:117-137, pmid:14283623.. ... 2 and 3 show examples of chromaffin cells after the cryofixation/freeze substitution was applied. DVs identified by their ...http://jcb.rupress.org/content/139/7/1709
Glycocalyx - Wikipedia
"Imaging the Endothelial Glycocalyx In Vitro By Rapid Freezing/Freeze Substitution Transmission Electron Microscopy". ...https://en.wikipedia.org/wiki/Glycocalyx
Mesosome - Wikipedia
By the mid to late 1980s, with advances in cryofixation and freeze substitution methods for electron microscopy, it was ... Dubochet J, McDowall AW, Menge B, Schmid EN, Lickfeld KG (1 July 1983). "Electron microscopy of frozen-hydrated bacteria". J. ...https://en.wikipedia.org/wiki/Mesosome
Scintillons - Wikipedia
Fast-freeze Fixation/Freeze Substitution) contain a large number of electron dense bodies around the cell periphery. These ... Cells observed under the electron microscope after a technique involving rapid freezing of the cells followed by substitution ... binding protein and luciferase to the scintillons ofGonyaulax polyedra revealed by double immunolabeling after fast-freeze ...https://en.wikipedia.org/wiki/Scintillons
Operation Big Bird - Wikipedia
The Swiss Federal Office of Police then issued a freeze order in substitution for the exceptional freezing order of the Federal ... 2, freezing all Marcos assets in the Philippines. March 15, 1986 De Guzman and Dagher return to Vienna, and was able to get the ... Scheller replied by telex from Credit Suisse, but informs De Guzman that the freeze order is still in place. De Guzman finally ... They were initially informed by Scheller that the Swiss authorities have put a "freeze order" on all the accounts linked to the ...https://en.wikipedia.org/wiki/Operation_Big_Bird
List of MeSH codes (E05) - Wikipedia
... freeze drying MeSH E05.200.500.620.760.160.260.270 --- freeze substitution MeSH E05.200.500.620.760.720 --- tissue fixation ... frozen sections MeSH E05.200.500.620.620 --- replica techniques MeSH E05.200.500.620.620.150 --- corrosion casting MeSH E05.200 ... freeze drying MeSH E05.200.750.600.760.160.260.270 --- freeze substitution MeSH E05.200.750.600.760.720 --- tissue fixation ... freeze drying MeSH E05.760.156.260.270 --- freeze substitution MeSH E05.760.833.230 --- blood preservation MeSH E05.760.833.445 ...https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)
Cooling bath - Wikipedia
A familiar example of this is the use of an ice/rock-salt mixture to freeze ice cream. Adding salt lowers the freezing ... Suitable freezing point. In some cases, a simple substitution can give nearly identical results while lowering risks. For ... This leads to a new, lower freezing point. With dry ice these baths will never freeze solid, as pure methanol and ethanol both ... Once the temperature reaches −41 °C, the acetonitrile will freeze. Therefore, dry ice must be added slowly to avoid freezing ...https://en.wikipedia.org/wiki/Cooling_bath
Winterization - Wikipedia
... battery as well as the substitution of winter-grade coolants and lubricants allows the vehicle to start and run in sub-freezing ... Fountains and other water features are often drained and sealed up so that water inside does not freeze or cause breakage of ... A properly maintained water feature containing fish can operate even in freezing temperatures. Steps should be taken to ensure ... This might involve a chemical treatment process, additional waterproofing/insulation, or even the total substitution of new ...https://en.wikipedia.org/wiki/Winterization
Wood frog - Wikipedia
Frogs can survive many freeze/thaw events during winter if no more than about 65% of the total body water freezes. Wood frogs ... have a series of 7 amino acid substitutions in the sarco/endoplasmic reticulum Ca2+-ATPase 1 (SERCA 1) enzyme ATP binding site ... The wood frog has garnered attention by biologists over the last century because of its freeze tolerance, relatively great ... doi:10.1890/0012-9658(1997)078[2279:EEOFWC]2.0.CO;2. Kenneth B. Storey (1997). "Organic solutes in freezing tolerance". ...https://en.wikipedia.org/wiki/Wood_frog
Degasification - Wikipedia
To complete the substitution, the solution should be stirred vigorously and bubbled for a long time. Because helium is not very ... In this laboratory-scale technique, the fluid to be degassed is placed in a Schlenk flask and flash-frozen, usually with liquid ... "Freeze-Pump-Thaw Degassing of Liquids" (PDF). University of Washington. ... The formation of gas bubbles when a liquid is frozen can also be undesirable, necessitating degassing. This process is called ...https://en.wikipedia.org/wiki/Degasification