*  Download Advances in Immunoassay Technology by N. Chiu, et. al. PDF - PinPoint Strategies Books
Gene silencing by RNA interference : technology and application. Content material: Gene silencing by means of RNA interference ... Kriegel, A. , Boustany, C. , & Blake, D. A. (2011). Single-chain variable fragment (scFv) antibodies optimized for ... DSC Study on the Polyacrylonitrile Precursors for Carbon Fibers. Journal of Material Science and Technology, Vol. ... Download Gene silencing by RNA interference : technology and by Muhammad Sohail PDF ...
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*  piRNAs, transposon silencing, and Drosophila germline development | JCB
The resulting piRNA-Ago3 complexes then cleave antisense piRNA precursors from clusters to produce RNA fragments that associate ... Precursor RNA fragments bound by Piwi could be trimmed to produce mature primary piRNAs (Fig. 1 B). ... The rhi locus is required for accumulation of putative piRNA precursor RNAs from the 42AB cluster, and the heterochromatin ... The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature. 453:803-806. doi:10.1038/nature07015. ...
  http://jcb.rupress.org/content/191/5/905
*  Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution | BMC Plant Biology | Full Text
The miRNAs 1-50 and 2-51 are located on a shared precursor that are separated by only one nucleotide and become processed in a ... As we have described here within, the coupled maturation of two miRNAs from a shared precursor has not been previously ... are regulatory RNA molecules that are specified by their mode of action, the structure of primary transcripts, and their ... We assume that this larger RNA fragment represents an incompletely processed precursor transcript. Thus, processing of the two ...
  https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-13
*  John Abelson - Wikipedia
He identified the enzymes that cleave RNA precursors into fragments, and elucidated the mechanisms by which fragments are ... His work has made possible an understanding of how genomic DNA can be converted to both messenger RNA and transfer RNA, ... List of RNA biologists His uncle, Philip Abelson, a physicist, was the longtime editor of Science and his aunt, Neva Martin ... This work involved both genetics and RNA sequencing (developed at that time by Fred Sanger) His first faculty position was in ...
  https://en.wikipedia.org/wiki/John_Abelson
*  Tracking miRNA precursor metabolic products and processing sites through completely analyzing high-throughput sequencing data |...
Highly expressed miRNA precursors could be entirely covered by various short RNAs and small RNA fragments with a hierarchical ... while other regions of precursors were found shorter RNAs or small fragments with fewer sequence counts. Furthermore, we ... Other regions of hsa-mir-24 also can be detected all kinds of mutual overlapping shorter RNAs (especially shorter RNAs with 6 ... strands into the RNA interference pathway. RNA 16:43-56PubMedGoogle Scholar ...
  https://rd.springer.com/article/10.1007/s11033-011-0950-8
*  Genetics 1 Flashcards - Cram.com
Stable RNAs are made from precursors, then processed into smaller fragments in the ________. ... Need RNA polymerase to make all cellular RNAs.. euk: rRNA is transcribed by RNA polymerase I in the nucleOlus.. tRNA ... prok: genes occur in multiple copies on precursor molecules that must be processed.. It's a ribozyme: contains RNA instead of ... 1) 5' to 3' removes RNA primers from Okazaki fragments. 2) 3' to 5' proofreads ...
  http://www.cram.com/flashcards/genetics-1-371376
*  RNA fragments may yield rapid, cccurate Cancer diagnosis | BioEd Online
He says it is a precursor to what he hopes will be a series of multiple-gene-signature cancer tests. "We are looking for ... Fragments of RNA that cells eject in fatty droplets may point the way to a new era of cancer diagnosis, potentially eliminating ... RNA fragments may yield rapid, cccurate Cancer diagnosis. January 31, 2013 This article courtesy of Nature News. ... Cancer tumor cells shed microvesicles containing proteins and RNA fragments, called exosomes, into cerebral spinal fluid, blood ...
  http://www.bioedonline.org/news/nature-news/rna-fragments-may-yield-rapid-accurate-cancer-diagnosis/
*  Altering α-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropism | Genetic...
Encoded by the small RNA fragment, the arenavirus glycoprotein precursor (GP-C) is post-translationally cleaved to yield GP1 ... one occurring in the viral polymerase encoded by the large RNA fragment and the other at position 260 of GP1 [14]. LCMV WE54 ... Arenaviruses are a family of single-stranded, enveloped, bisegmented RNA viruses that include the Old World arenaviruses ... Optimization of feline immunodeficiency virus vectors for RNA interference. J Virol. 2006, 80: 9371-9380.PubMed CentralView ...
  https://gvt-journal.biomedcentral.com/articles/10.1186/1479-0556-9-8
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Kotzot, D. (A) The DNA polymerase of the upstream fragment meets the RNA primer from the next precursor fragment down-stream. ... Researchers use hybridization to determine the location of particular DNA sequences in a library of frag- ments. ...
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*  RNA Processing and Synthesis
... bacteria process the primary transcripts for r RNA and t RNA. The newly synthesized RNA molecule... ... end of the precursor molecule by RNase P that generates a single fragment and a correct 5' end. ... r RNA processing. Bacterial ribosomes consists of 16s r RNA (1541 nucleotides), 23s r RNA (2904 nucleotides) and 5s r RNA (120 ... Processing of t RNA, r RNA and small RNAs. To form physiologically active RNA molecules, bacteria process the primary ...
  https://www.ukessays.com/essays/biology/processing-rna-rna-rnas-4329.php
*  Gap Junctions/Hemichannels Modulate Interkinetic Nuclear Migration in the Forebrain Precursors | Journal of Neuroscience
We used the short hairpin RNA (shRNA) Target finder at GenScript to design shRNA sequences against the mouse Cx43, and the ... shRNA fragment was inserted into the pRNAT-U6.2/Lenti vector. The targeting sequences are as follows: Cx43, 5′- ... Neural precursors release ATP via hemichannels. VZ precursors express P2Y1 receptors and display Ca2+ increase in response to ... VZ precursors were examined at E16.5. A , In the control brain, the EGFP+ VZ precursors identified by the foot-like process ...
  http://www.jneurosci.org/content/30/12/4197
*  Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a...
XMRV RNA was detected in prostatic secretions of some men with prostate cancer. The fact that the precursor of SEVI is produced ... We show that amyloidogenic fragments known as semen-derived enhancer of virus infection (SEVI) originating from prostatic acid ... Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a ...
  http://www.uniprot.org/citations/19403677
*  ArcZ RNA - Wikipedia
This precursor is unstable and is processed into an abundant fragment ~58 nucleotides which represents the 3' end of the ... In molecular biology the ArcZ RNA (also known as RyhA and SraH) is a small non-coding RNA (ncRNA). It is the functional product ... ArcZ is an Hfq binding RNA that functions as an antisense regulator of a number of protein coding genes. This non-coding RNA ... "Genome-wide identification of Hfq-regulated small RNAs in the fire blight pathogen Erwinia amylovora discovered small RNAs with ...
  https://en.wikipedia.org/wiki/ArcZ_RNA
*  Epstein-Barr virus small nucleolar RNA 1 - Wikipedia
... also been proposed that this snoRNA may act as a miRNA-like precursor that is processed into 24-nucleotide-sized RNA fragments ... Page for human herpesvirus 1 small nucleolar RNA at Rfam Page for EBER1 at Rfam Page for v-snoRNA1 at Rfam Page for IRES EBNA ... "Expression and processing of a small nucleolar RNA from the Epstein-Barr virus genome" (Free full text). PLoS Pathogens. 5 (8 ... Epstein-Barr virus stable intronic sequence RNAs Hutzinger, R.; Feederle, R.; Mrazek, J.; Schiefermeier, N.; Balwierz, J.; ...
  https://en.wikipedia.org/wiki/Epstein%E2%80%93Barr_virus_small_nucleolar_RNA_1
*  Genes | Free Full-Text | Hints of tRNA-Derived Small RNAs Role in RNA Silencing Mechanisms | HTML
They could represent the most primitive small RNA pathways from which the well-known canonical RNA silencing pathways reported ... tRNA-derived small RNAs represent one of these novel members that are, surprisingly, among the most conserved class of small ... a growing number of novel classes of small RNA, other than miRNAs or siRNA, has emerged, which appear as new actors in gene ... fragments derived from small nucleolar RNA (snoRNA) [37] and tRNAs [5] which undergo cleavage of the precursor RNA before ...
  http://www.mdpi.com/2073-4425/3/4/603/htm
*  The Developmental Transcriptome of the Mosquito Aedes aegypti, an Invasive Species and Major Arbovirus Vector
piRNAs are generated from a variety of different precursors, including long single-stranded RNAs, and complex and repetitive ... The unit of measurement is fragments per kilobase of exon per million fragments mapped (FPKM). ... Total RNA was extracted from ovaries of NBF females, and PBM, at 24-, 48-, and 72-hr time points. RNA was also extracted from 0 ... Small RNAs from 0−2 hr and 2−4 hr embryos should consist of maternally expressed RNAs deposited into the egg but not include ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3755910/?lang=en-ca
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The resulting fragments are components of the RNA-induced silencing complex (RISC), directing it to the cognate target mRNA. ... The short double-stranded RNAs originate from longer double stranded precursors by the activity of Dicer, a protein of the ... The potent ability of small interfering (si)RNAs to inhibit the expression of complementary RNA transcripts is being exploited ... Immunology, Issue 52, SELEX (Systematic Evolution of Ligands by EXponential enrichment), RNA aptamer, HIV-1 gp120, RNAi (RNA ...
  https://www.jove.com/visualize/abstract/23717655/local-applications-myostatin-sirna-with-atelocollagen-increase
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The resulting fragments are components of the RNA-induced silencing complex (RISC), directing it to the cognate target mRNA. ... Cleavage of the precursor RNA normally occurs 10-30 nt downstream from the consensus polyA site (AAUAAA) after the CA ... RNA, High-throughput SHAPE, Capillary electrophoresis, RNA structure, RNA probing, RNA folding, secondary structure, DNA, ... RNAi is induced by short double-stranded RNAs also known as small interfering RNAs (siRNAs). The short double-stranded RNAs ...
  https://www.jove.com/visualize/abstract/25141304/exploring-microrna-like-small-rnas-filamentous-fungus-fusarium
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The resulting fragments are components of the RNA-induced silencing complex (RISC), directing it to the cognate target mRNA. ... Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA- ... RNAi is induced by short double-stranded RNAs also known as small interfering RNAs (siRNAs). The short double-stranded RNAs ... RNA-sequencing (RNA-Seq) has been increasingly used to replace microarray for gene expression studies. We report here a ...
  https://www.jove.com/visualize/abstract/22870320/optimized-pcr-conditions-increased-shrna-fold-representation-improve
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The resulting fragments are components of the RNA-induced silencing complex (RISC), directing it to the cognate target mRNA. ... The short double-stranded RNAs originate from longer double stranded precursors by the activity of Dicer, a protein of the ... RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes ... RNAi is induced by short double-stranded RNAs also known as small interfering RNAs (siRNAs). ...
  https://www.jove.com/visualize/abstract/24223931/identification-wee1-like-kinase-gene-essential-for-procyclic
*  Starvation-Induced Stress Response Is Critically Impacted by Ceramide Levels in Caenorhabditis elegans | Genetics
Ceramides were detected using precursor ion scans in the positive mode for the 250.3 μ fragment of D17:1 sphingoid bases. For ... RNA isolation. Synchronized L1 worms were obtained as above. Animal samples were collected 30 hr after bleaching. Total RNA was ... 2009 RNA Pol II accumulates at promoters of growth genes during developmental arrest. Science 324(5923): 92-94. ... The hyl-1 genomic DNA fragment from the translational start codon to the stop codon was amplified from N2 genomic DNA. It was ...
  http://www.genetics.org/content/205/2/775.full
*  Reduced pachytene piRNAs and translation underlie spermiogenic arrest in Maelstrom mutant mice | The EMBO Journal
... long fragments of piRNA precursors formed a few prominent peaks while small and mid‐sized RNA reads formed numerous peaks ... The RNA pellets were resuspended in 50 μl (RNA pellet from lysate) or 15 μl (RNA pellet from IP samples) of water. The RNA ... Small RNA sequencing.. For small RNA deep sequencing, 16 μg of Mael129+/+ and 10 μg Mael129−/− of total RNA extracted from the ... RT-PCR analysis of piRNA precursors in sucrose gradient fractions. RNA extraction from sucrose gradients.. For RT-PCR analysis ...
  http://emboj.embopress.org/content/33/18/1999
*  What's hot today: Current papers in developmental biology and gene function
In addition, they can play diverse regulatory roles on gene expression, acting as precursors of smaller fragments able to ... The exosome acts on different RNA substrates and plays important roles in RNA metabolism. The fact that short non-coding RNAs ... perturb thermal responsiveness in RNA editing profile for a particular RNA structure. These results suggest that the RNA ... The acal locus coded for a conserved, long, non-coding, nuclear RNA. Long non-coding RNAs are an abundant and diverse class of ...
  http://www.sdbonline.org/sites/fly/aimain/model-organisms-february2015.htm
*  From Gene to Protein: Information Transfer in Normal and Abnormal Cells - 1st Edition
Identification of Putative Precursors of Ovalbumin and Ovomucoid Messenger RNAs. Purification of mRNA Guanylyl Transferase from ... Cloning of Fragments of the Sea Urchin Histone Gene Cluster with SV40 DNA as a Vector. Regulation by Metabolites of ... Splice Patterns of Adenovirus and Adenovirus-SV40 Mosaic RNA's. Processing of Bacteriophage T7 RNAs by RNase III. Regulation of ... Characterization of a Virion-Associated RNA Polymerase from Killer Yeast. In Vitro Transcription of Ad2 DNA by Eukaryotic RNA ...
  https://www.elsevier.com/books/from-gene-to-protein-information-transfer-in-normal-and-abnormal-cells/russell/978-0-12-604450-8
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Short hairpin RNAs, precursors to siRNA, can be expressed using lentivirus, allowing for RNAi in a variety of cell types. ... The resulting fragments are components of the RNA-induced silencing complex (RISC), directing it to the cognate target mRNA. ... RNA viruses use RNA dependent RNA polymerases to replicate their genomes. The intrinsically high error rate of these enzymes is ... RNAi is induced by short double-stranded RNAs also known as small interfering RNAs (siRNAs). The short double-stranded RNAs ...
  https://www.jove.com/visualize/abstract/26147769/assorted-processing-synthetic-trans-acting-sirnas-its-activity