Loading...
*  Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50 C. ... most preferably the DNA polymerase I from Thermus aquaticus (Taq polymerase and the Stoffel fragment of Taq polymerase are the ... plus cells carrying integrated viral genomes or vital particles. ... After DNA amplification, all digoxigenin-tagged DNA which was ... "Single-stranded DNA binding protein" (SSB) refers to a polypeptide which binds to single-stranded DNA more tightly than to ...
  http://www.google.es/patents/US5538871
*  Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... A. DNA probe for 23s RNA. The DNA probe is an EcoRI/BglII fragment from the rrnD operon which codes for 23s RNA in E. coli ... Diagnosis of viral infections by detection of genomic and infectious viral dna by molecular combing. ... C. Preparation of Antibody to DNA.RNA Hybrid. A DNA.RNA hybrid is prepared by transcription of φX174 virion DNA with RNA ...
  http://www.google.ca/patents/US5200313
*  DNA restriction enzymes facts, information, pictures | Encyclopedia.com articles about DNA restriction enzymes
Make research projects and school reports about DNA restriction enzymes easy with credible articles from our FREE, online ... and pictures about DNA restriction enzymes at Encyclopedia.com. ... the viral DNA is fragmented. Destruction of the viral DNA ... These blunt ended fragments can be joined to any other DNA fragment with blunt ends, making these enzymes useful for certain ... Typically, bacterial DNA in the form of a plasmid (a small, circular DNA molecule) is joined to another piece of DNA (a gene) ...
  https://www.encyclopedia.com/science-and-technology/biology-and-genetics/genetics-and-genetic-engineering/dna-restriction
*  Patent US6376234 - Method of inserting viral DNA into plant material - Google Patents
Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific ... which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon ... The present invention relates to a novel method of inserting viral DNA, ... It has ready proved possible in many cases to insert selected DNA fragments into viral DNA and then, together with the virus, ...
  http://www.google.com/patents/US6376234?dq=7,136,842
*  How ex-Penn scientist's hunch led to cervical vaccine
Using the lab's latest HPV - isolated from laryngeal warts - he found a fragment of viral DNA in a cervical cancer biopsy. ... Cloning provided a fuller picture, a complete genetic sequence that they could use to look for the viral DNA in the biopsies, ... This rarely yielded virus particles, but even when it did, zur Hausen's team couldn't tease out the prize - pure viral DNA. ... His understanding of 'viral DNA integration and persistence' would shape his insights into the papillomavirus. ...
  http://www.philly.com/philly/business/jobs/industries/healthcare/20081006_UNCOVERING_A_VIRAL_VILLAIN.html
*  Avian herpesvirus amplicon as a eucaryotic expression vector - Patent # 4996152 - PatentGenius
DNA fragments (seeds) having the characteristics of amplicons, which are useful for amplifying genes of interest, have been ... amplicon: a fragment of viral DNA which contains all cis-acting functions required for DNA replication. In the presence of ... total pA5 DNA; (2) linear viral DNA isolated from pA5 after EcoRI digestion; (3) the viral DNA of (2) ligated to form circles; ... Cloning Viral DNA into pUC18. In order to characterize the amplified 4-kb EcoRI fragment, total DNA isolated from cells ...
  http://www.patentgenius.com/patent/4996152.html
*  The key to defeating superbugs? Designer viruses could be the new antibiotics
With both these observations it quickly became clear that bacteria were introducing viral DNA fragments into their own genome ... which guides the DNA-cutting protein called Cas9 and tells it where to chop off viral DNA. ... In 2012, a German team identified all the pieces and showed how exactly bacteria transcribe viral DNA into a short RNA - ... the Cas9 protein to target genes that endow the bug antibiotic resistance or other harmful properties by embedding viral DNA. ...
  https://www.rawstory.com/2014/10/the-key-to-defeating-superbugs-designer-viruses-could-be-the-new-antibiotics/
*  Recombinant DNA Research and Viruses | SpringerLink
The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacter ... The development of recombinant DNA technology has made a marked impact on molecular virology. ... The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led ... The Cloning and Sequencing of Sites of Linkage between Adenovirus DNA and Cellular DNA: Recombination of Foreign DNA with the ...
  https://link.springer.com/book/10.1007%2F978-1-4613-2565-9
*  Association of a Geminivirus with a Leaf Curl Disease of Sunn Hemp ( Crotalaria juncea) in India | SpringerLink
The PCR-amplified viral DNA fragments were further characterized by Southern hybridization with a geminivirus probe consisting ... amplification of DNA fragments of expected sizes with three pairs of degenerate geminivirus primers. ... Restriction fragment length polymorphism analysis of a PCR-amplified CP fragment revealed that the geminivirus from sunn hemp ... of Indian tomato leaf curl virus genome following a simple method of concentrating super coiled replicative form of viral DNA. ...
  https://link.springer.com/article/10.1023%2FA%3A1024237322397
*  bt toxin | Food Democracy Now
That's certainly not proven to be safe, indeed the hazards are numerous: protein encoding viral DNA fragments, herbicide ...
  http://www.fooddemocracynow.org/tags/bt-toxin
*  Microbiology Society Journals | The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus...
... but not to other regions of the viral chromosome in vivo. In contrast, in vitro LANA-DNA binding was much less specific. To ... order to identify the in vivo-binding sites for LANA on the whole KSHV genome and to analyse the function of this protein-DNA ... The latency-associated nuclear antigen (LANA) has been shown to tether viral DNA fragments to chromosomes and is proposed to ... The latency-associated nuclear antigen (LANA) has been shown to tether viral DNA fragments to chromosomes and is proposed to ...
  http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18940-0
*  Inter Research » AB » v14 » n3 » p223-232
We analyzed a fragment of viral DNA from lobsters collected in the Florida Keys, which provided the first insight into the ... The variation in viral sequences, both within and between individual lobsters, indicates that lobsters can be infected with ... multiple viral strains. Laboratory experiments in which uninfected lobsters were serially exposed to lobsters inoculated with ...
  http://www.int-res.com/abstracts/ab/v14/n3/p223-232/
*  Medical Consumers Beware: 'First do no harm' does not apply at the FDA - SaneVax, Inc.
Viral DNA and bacterial DNA fragments firmly bound to aluminum salts are more of a concern than yeast DNA, particularly when it ... Any way you slice it, HPV DNA is viral DNA - it need not be the complete virus to be viral DNA. ... Undegraded viral and plasmid DNA fragments are known to activate macrophages, causing them to release tumor necrosis factor, a ... We must emphasize that what was discovered in the Gardasil® vaccine and in Jasmine's samples are viral DNA fragments, not the ...
  http://sanevax.org/medical-consumers-beware-first-do-no-harm-does-not-apply-at-the-fda/
*  Flu Vaccines and the Risk of Cancer
There is a real risk of residual retinal DNA and stray viral fragments from the animal tissues getting into flu shots. DNA ... The entire process, from viral selection to viral harvest, can take up to nine months [2]. With the potential for a pandemic ... by researchers who worry that the stray DNA is being incorporated into the recipient's DNA. FDA regulations insist on the " ... The annual flu shot contains three viral strains: two type influenza A viruses and one type influenza B. Most commonly, two of ...
  http://www.i-sis.org.uk/fluVaccinesCancerRisks.php
*  Cold Sores - RxMed.com
As a result, virus «fleeing», and fragments of viral DNA migrate into trigeminal ganglion (nerve node or plexus of trigeminal ... integrating viral particles into human genetic code, and human body reacts by activating immune defense mechanisms. ...
  https://www.rxmed.com/illness/cold-sores
*  The oncogene of BAV-3 as a mutagen | Journal of Cell Science
Fragment C, pBR325 without viral sequences and DNA from normal Syrian hamster cells did not have any mutagenic effect. We also ... Fragment D located between 3.6 and 19.7 map units, contains the viral oncogene, fragment C, located between 44.3 and 63.7 map ... the mutagenic and transforming effects of viral DNA and show that both activities are mapped in the same region of viral DNA - ... looked at the combined action of the viral DNA fragments and the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), ...
  http://jcs.biologists.org/content/78/1/97
*  Erythema multiforme - Cancer Therapy Advisor
... where they transfer viral DNA fragments (including those that encode viral DNA polymerase) to epidermal keratinocytes. The ... Proposes that viral DNA fragments are transported to the skin by peripheral blood mononuclear cells, which then triggers ... Expression of HSV genes encoded within DNA fragments deposited on the skin leads to recruitment of HSV-specific CD4+ Th1 cells ... Infections (viral, bacterial, fungal) account for approximately 90% of cases, with HSV as the most commonly identified ...
  https://www.cancertherapyadvisor.com/dermatology/erythema-multiforme/article/594964/
*  Spliceosome - Wikipedia
When larger fragments of viral DNAs were used, forked structures of looped out DNA were observed when hybridized to the viral ... gene structure was found when adenoviral mRNAs were hybridized to endonuclease cleavage fragments of single stranded viral DNA. ... or present in several distinct segments along the DNA molecule.[2][3] The coding regions of the gene are separated by non- ... 2] It was observed that the mRNAs of the mRNA-DNA hybrids contained 5' and 3' tails of non-hydrogen bonded regions. ...
  https://en.m.wikipedia.org/wiki/Spliceosome
*  Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation...
To overcome these limitations, we used synthetic biology tools to capture genomic fragments from viral DNA and assemble full- ... PCR for viral DNA.Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using ... Expected DNA band sizes were observed. Blue diamonds indicate vector DNA bands, and red circles indicate fragment 4 DNA bands ... DNA from positive transformants was isolated and transformed into E. coli. DNA was isolated from E. coli, and the viral genome ...
  https://msphere.asm.org/content/2/5/e00331-17
*  Link identified between virus recognition, destruction in bacterial immune system | EurekAlert! Science News
A bacterium gains immunity against a virus through a sophisticated process of acquiring a fragment of the viral DNA and ... A CRISPR-associated protein known as Cas9 destroys invading viral DNA and has been co-opted as a tool for programmable genome ... In the study, the team describes the basic set of machinery that is required to obtain a specific fragment of viral sequence ... "That Cas9 is involved in both processes represents a major step forward in understanding how bacteria discern which DNA to cut ...
  https://www.eurekalert.org/pub_releases/2015-03/uog-lib030215.php
*  Patent US7541048 - Coating compositions for bioactive agents - Google Patents
... including plasmid DNA and viral fragments, along with associated promoters and excipients. Examples of other molecules that can ... Anti-viral agents are substances capable of destroying or suppressing the replication of viruses. Examples of anti-viral agents ... Examples of suitable gene therapy agents include a) therapeutic nucleic acids, including antisense DNA and antisense RNA, and b ... The term antibody includes whole antibodies or fragments thereof.. Cell response modifiers are chemotactic factors such as ...
  http://www.google.co.uk/patents/US7541048
*  Patent US20050220843 - Coating compositions for bioactive agents - Google Patents
... including plasmid DNA and viral fragments, along with associated promoters and excipients. Examples of other molecules that can ... Anti-viral agents are substances capable of destroying or suppressing the replication of viruses. Examples of anti-viral agents ... Examples of suitable gene therapy agents include a) therapeutic nucleic acids, including antisense DNA and antisense RNA, and b ... DNA that encodes for the production of any of these proteins, antisense molecules, androgenic receptor blockers and statin ...
  http://www.google.com/patents/US20050220843?dq=6446111
*  Vaccination's Dilemma: 'Unavoidably Unsafe' at Any Dose | Wake Up World
Isn't it therefore time to pause and review the huge amount of DNA contamination, known and unknown viral genetic fragments ... the FDA convened a non-public regulatory meeting to review the health hazards of undesirable viral DNA fragments and protein ... Science still has very limited knowledge about how bacterial and viral genes interact with our own DNA, gene regulation, and ... This viral concoction combines bovine rotavirus strains that causes diarrhea in cows with viral strains causing diarrhea in ...
  https://wakeup-world.com/2016/06/05/vaccinations-dilemma-unavoidably-unsafe-at-any-dose/
*  State of the Union Illuminati imprisoning Little girl as she walked down the ramp of a military vehicle called an A.P.C.
It was reconfirmed that vaccines are "widely contaminated by viral and DNA genetic code fragments, many viruses and proteins. ... The FDA's reassurance that the ALV RT activity was safe is based on laboratory observations that there was no viral-human DNA ... Andrew Lewis, then head of the DNA Virus Laboratory in the Division of Viral Products confirmed that: "All the egg-based ... Children today are inoculated with many vaccines before entering school, each with unique DNA and viral contaminants due to the ...
  http://thewe.cc/weplanet/news/americas/us-/state_of_the_union-2010-2011.html
*  BioNews - 919
Fascinatingly, Professor Mojica's research uncovered how bacterial cells store and save fragments of viral DNA within the ... The study, published in Nature, marks the first time that the nuclear DNA of human embryos has been edited in the UK, where use ... Robin, who provided DNA and a womb, is Mary's mother; Julia, who brought Mary up, isn't. Someone commissioning surrogacy ticks ... The risks of off-target DNA mutations that could lead to unwanted genetic complications (BioNews 837) must be considered, and ...
  http://www.bionews.org.uk/page.asp?obj_id=889996&CView=188645