Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging | Journal of Cell Science
This aberration rate reflects the intensity of DNA damage. As chromosome aberrations we counted breaks, fragments, ring ... we also calculated the incidence of chromosome aberrations, i.e. aberration rate, which represents the total number of ... S3A), oocytes with normal paternal chromosomes (supplementary material Fig. S3B), and aberration rate (supplementary material ... Sperm chromosome analysis. Chromosome preparation and analysis were performed as described by us before (Yamauchi et al., 2010 ...http://jcs.biologists.org/content/126/3/803
Induction of chromosome aberrations in human lymphocytes by technetium-99m. In vitro and in vivo studies
The induction by ionizing radiations of unstable structural chromosome aberrations (dicentrics, centrics, and fragments) in ... Induction of chromosome aberrations in human lymphocytes by technetium-99m. In vitro and in vivo studies Bibliographie ... The distribution of unstable chromosome aberrations after in vitro 99mTc irradiation follows a Poisson law. We observed no ... Further investigations examining the stable aberrations such as translocations are presently being carried out in our ...http://www.irsn.fr/EN/Research/publications-documentation/Publications/DRPH/SRBE/Pages/Induction-of-chromosome-aberrations-in-human-lymphocytes-by-technetium-99m-In-vitro-and-in-vivo-studies-516.aspx
Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients...
Chromosome aberrations. Table 1 presents the number of unstable CA (dicentrics and fragments) in lymphocytes, as well as their ... Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the ... Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients ... For the chromosome aberration studies, 0.3 mL of the blood samples were cultured for 48 h, in a humidified atmosphere ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300021&lng=en&nrm=iso&tlng=en
Okazaki Fragments - Biological Function | Technology Trends
... "chromosome aberrations". Severe defects of Okazaki fragment maturation may halt DNA replication and induce cell death. However ... Okazaki Fragments - Biological Function. Biological Function. Although synthesis of the lagging strand involves only half the ... Processing of Okazaki fragments is therefore very common and crucial for DNA replication and cell proliferation. ... During this process, RNA and DNA primers are removed, allowing the Okazaki fragments to attach to the lagging DNA strand. While ...http://www.primidi.com/okazaki_fragments/biological_function
... higher incidence of chromosome aberrations (acentric fragments and chromatid exchanges) than in control individuals. Annual ...http://netbiologen.dk/artikel/beskidtedusin.htm
Multiple structural aberrations and physical mapping of rye chromosome 2R introgressed into wheat | Springer for Research &...
Multiple structural aberrations produced by chromosome breakage and reunion not only provide new germplasm for enriching ... Ma ZQ, Sorrells ME (1995) Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. ... Potential use of these stable aberrations and the colinearity of chromosome 2R with corresponding chromosomes in the other ... gametocidal chromosome action and irradiation. A total of 88 chromosome 2R aberrations were identified in 65 plants. From the ...https://rd.springer.com/article/10.1007/s11032-015-0333-2
Okazaki fragments - Wikipedia
... "chromosome aberrations". Severe defects of Okazaki fragment maturation may halt DNA replication and induce cell death. However ... Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA ... Prokaryotes have Okazaki fragments that are quite longer than those of eukaryotes. Eukaryotes typically have Okazaki fragments ... Okazaki fragments that are not ligated could cause double-strand-breaks, which cleaves the DNA. Since only a small number of ...https://en.wikipedia.org/wiki/Okazaki_fragments
Protective Effects of Hydrogen against Low-Dose Long-Term Radiation-Induced Damage to the Behavioral Performances,...
Chromosome aberrations observed in bone marrow metaphases were scored as follows: (1) chromosome- and chromatid-type fragments ... Chromosome Aberration. The bone marrow chromosome aberration (CA) investigation was conducted as reported previously with minor ... chromosome deletion, and (h) chromosomes with exchanged fragments. Arrows indicate specific aberrations. Pictures were taken at ... chromosome fragments, (e) chromosome with dicentrics and the accompanying fragments, (f) Robertsonian translocation, (g) ...https://www.hindawi.com/journals/omcl/2016/1947819/
A MODEL OF RADIATION-INDUCED CELL KILLING: INSIGHTS INTO MECHANISMS AND APPLICATIONS FOR HADRONTHERAPY | IRIS UNIPV
... it was assumed that chromosome aberrations derive from µm-scale mis-rejoining of chromatin fragments, which in turn originate ... and that these lesions lead to chromosome aberrations via mis-rejoining of chromatin fragments at the µm-scale. Following ... it was assumed that chromosome aberrations derive from µm-scale mis-rejoining of chromatin fragments, which in turn originate ... and that these lesions lead to chromosome aberrations via mis-rejoining of chromatin fragments at the µm-scale. Following ...https://iris.unipv.it/handle/11571/721421
Urinary Naphthol Metabolites and Chromosomal Aberrations in 5-Year-Old Children | Cancer Epidemiology, Biomarkers & Prevention
... and unidentified green chromosomes were presumed to be composed of either 3, 5, or 6p. Fragments without centromeres and ... Chromosome aberration scoring by WCP-FISH. Aberrations are scored in T lymphocytes that have a half-life of up to a year in ... Rapid human chromosome aberration analysis using fluorescence in situ hybridization. Int J Radiat Biol 1989;56:35-44. ... and aberration type. The aberration and translocation frequencies were calculated from the number of chromosomal aberrations ...http://cebp.aacrjournals.org/content/21/7/1191.long
Sirt1 ablation promotes stress-induced loss of epigenetic and genomic hematopoietic stem and progenitor cell maintenance | JEM
... chromosome fragments, gaps, and breaks (Fig. 5 F). The frequency of individual aberrations observed in cultures from three ... The frequency of total metaphases containing DNA aberrations is shown. DAPI images depict examples of individual aberrations. ... fragments, dicentric chromosomes, centromere fucions/robertsonian translocations, and radials. Same scoring standard were ... Metaphase aberration assay.. Metaphase spreads were prepared and analyzed as described previously (Biswas et al., 2011). In ...http://jem.rupress.org/content/210/5/987
Evaluation of the Genotoxic Potential against -Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of...
A dose-dependent increase of chromosome aberrations was also observed in the Allium cepa assay. The abnormalities scored were ... which is the product of the single-stranded cleavage of supercoil DNA and quantified as fragmented separate bands on agarose ... stickiness, c-mitosis, bridges, and vagrant chromosomes. Micronucleated cells were also observed at the interphase. The results ...https://www.hindawi.com/journals/ecam/2013/925380/abs/
Hepatitis C Virus Inhibits DNA Damage Repair through Reactive Oxygen and Nitrogen Species and by Interfering with the ATM-NBS1...
About 23% of metaphase chromosomes contained aberrations at passage 38 (Fig. 5D). Multiple chromosomal aberrations could be ... These aberrations included nonreciprocal clonal rearrangements (e.g., chromatid breaks, acentric fragments, and duplications; ... 5A, 5B). NAC, BSO, and 1400W treatment of 293core cells significantly reduced total aberrations per chromosomes (Fig. 5E). ... 2F). The propensity for chromosome loss was greater than that for chromosome gain (Fig. 2F, 2G). As cancer cell lines, such as ...http://www.jimmunol.org/content/185/11/6985.long
DNA Vaccination Controls Her-2+ Tumors that Are Refractory to Targeted Therapies | Cancer Research
Similarly, NCCAs and chromosome fragments were found in TUBO, not Bam1a cells, showing more severe chromosomal aberrations in ... Clonal and non-clonal chromosome aberrations and genome variation and aberration. Genome 2006; 49: 195-204. ... shows chromosomal aberrations in neu-expressing tumor lines, including CCAs, NCCAs, and chromosome fragments. ... When comparing D2F2/neu with D2F2 cells, they shared one CCA at t(11;16) and chromosome fragments X, 13, 3, and 1 ( Table 1), ...http://cancerres.aacrjournals.org/content/68/18/7502.long
... to aberrations than the diabetic animals (D-group).. sperm abnormalities and chromosome aberrations [14, 16 It is worth noting ... display four amplified fragments while 610 bp fragments for A-group. Also, samples treated with Eight fragments in the control ... DNA damage and chromosomes aberrations. The published remains grossly unsatisfactory. Thus, it is vital to. clinical and / or ... And Chromosomes Aberrations. Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203 Jeddah ...http://medpdffinder.com/i/ijaset.com1.html
Polycentric chromosome - Wikipedia
... as a result the chromosome is fragmented, which causes the death of the cell. In some algae polycentric chromosome are found ... Polycentric chromosome is produced by chromosomal aberrations like deletion, duplication and trans location. It is specifically ... The polycentric chromosome is when more than 1 Centromere are present in a chromosome. ... In those organism the chromosome instead of remaining at specific position remain dispersed throughout the chromosome. This ...https://en.wikipedia.org/wiki/Polycentric_chromosome
The ageing epigenome: damaged beyond repair?
2007) and can result in detrimental chromosomal aberrations such as end-to-end fusions or fragmented chromosomes (Feldser et al ... The SAC ensures faithful chromosome segregation and is triggered by defects in chromosome alignment or chromosomal DNA breaks ... 2008). Telomeres are composed of TTAGGG repeats that can expand over ,10 kb at the ends of each chromosome. They are maintained ... 2008). Lack of SIRT1 causes an increase in chromosomal aberrations in response to ROS or ionizing irradiation and mice with ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2696802/
McCabism: April 2010
The fragments from the two broken chromosomes are then exchanged, rather than spliced back to the correct chromosomes from ... It is generally thought that such chromosome aberrations occur because the two separate chromosomes both suffer double-strand ... For example, chromosome translocations occur when genetic material is exchanged between two different chromosomes. ... which may be the number of chromosome aberrations, DNA mutations, or the probability of cell death. For radiation of a fixed ...http://mccabism.blogspot.com/2010/04/
CyDAS User Guide: Develop a Karyogram
Ring chromosomes are linearized. Marker chromosomes are shown as chromosomes of unknown origin ("?"). For unknown fragments or ... for chromosomes derived from an aberration) is displayed. Hovering over the "#", information on the chromosome (the aberration ... For each aberration, all derivative chromosomes derived from the aberration are calculated. I.e. a ". t(9;22)(q34.12;q11.23). ... Among these are: not exact number of chromosomes, sex chromosomes, some description errors in the aberrations. ...http://www.cydas.org/Docs/CyDASUserGuide/Karyogram.html
Immortal, telomerase-negative cell lines derived from a Li-Fraumeni syndrome patient exhibit telomere length variability and...
... and the principal types of aberrations were breaks, double minute chromosomes and dicentric chromosomes. In addition, ... All five immortal cell lines exhibited variability in the mean length of terminal restriction fragments (TRFs). Subclones of ... Chromosome aberrations were observed at high frequencies in these cell lines including the subclones and re-subclones, ... Keywords: AFB1, aflatoxin B1; ALT, alternative lengthening of telomeres; DM, double minute chromosomes LFS, Li-Fraumeni ...http://oxfordindex.oup.com/view/10.1093/carcin/bgg024
Inherited sterility in insects - Wikipedia
The plates also reduce the risk of lethality caused by the formation of dicentric chromosomes, acentric fragments, and other ... unstable aberrations. The F1 sterile progeny produced in the field enhance the efficacy of released partially sterile males, ... Meiotic pairing of sex chromosome fragments and its relation to atypical transmission of a sex-llinked marker in Ephistia ... The acentric fragment is frequently lost, while the dicentric fragment forms a bridge at anaphase leading to another ...https://en.wikipedia.org/wiki/Inherited_sterility_in_insects
Mechanisms of DNA damage-Induced Cell Death Radiobiology ppt download
33 Asymmetric chromosome aberrations from DSBs Occur primarily in unreplicated cells (G1) Can result in direct loss of genetic ... chromatin fragment) Necrotic death -Loss of energy leads to ion imbalance across plama membrane (Na/K ATPase) Mitotic death - ... 31 Radiation-Induced aberrations In metaphase spreads 32 FISH to Identify Aberrations Lymphocytes exposed to 4 Gy Nataragan MR ... 27 Radiation-Induced Chromosome Aberrations Exact mechanisms not established, but involves DNA damage and Misrepair ATM fragile ...http://slideplayer.com/slide/4382444/
Tumor fragment F329 came from a patient who displayed a high response to FOLFOX treatment, but because of L-OHP-induced ... For example, chromosome number varied between 52 and 56 for CT320, between 49 and 55 for CT320X6, from 65 to 136 for CT329, and ... Karyotypes showed a pattern of aberrations indicating that these cell lines share a core of karyotype alterations and display ... Using 32 tumor fragments from patients with colon cancer, the present study shows that prior xenograft leads to more efficient ...http://cancerres.aacrjournals.org/content/67/1/398
Stop the radiation!: Lynn Surgalla: Biolelectromangnetics radiation DANGERS
and specific chromosomal aberrations (acentric fragments and dicentric chromosomes). The results of the study indicate that ... researchers also reported finding fragmented nuclei and fragmented chromosomes among the cells that had been exposed to ... chromosome aberrations in human lymphocytes exposed to microwave radiation in vitro. Garaj-Vrhovac V, Fucic A, Horvat D Mutat ... chromosome aberration test and of the micronucleus test were comparatively analyzed. The values obtained showed a positive ...http://lowlevelradiation.blogspot.com/2008/03/lynn-surgalla-biolelectromangnetics.html
Apaf1 plays a pro-survival role by regulating centrosome morphology and function | Journal of Cell Science
... it is known that spindle aberrations can cause chromosome instability (Fukasawa, 2007). In fact, among ETNA−/− cells we have ... a combination of Drp1-dependent fragmented mitochondria, low levels of anti-apoptotic proteins, fast cytochrome c release and ... which suggests chromosome segregation defects (see supplementary material Fig. S1). Gain or loss of chromosomes greatly ... 1987). The chromosome cycle and the centrosome cycle in the mitotic cycle. Int. Rev. Cytol. 100, 49-92. ...http://jcs.biologists.org/content/124/20/3450
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