Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary...
Khodjakov, A., Cole, R. W., McEwen, B. F., Buttle, K. F. and Rieder, C. L. ( 1997). Chromosome fragments possessing only one ... An increasing number of studies have shown that abnormalities such as aneuploidy and whole-chromosome loss of heterozygosity ... that in untreated human primary fibroblasts the frequency of anaphase lagging chromosomes for all the 23 chromosome pairs was ... of which 94 displayed lagging chromosomes, for a total of 130 chromosome loss events for the two chromosomes under study. The ...http://jcs.biologists.org/content/115/3/507
Karyotype analysis of paired cell lines. Using classic cytogenetic techniques, a study of chromosomal abnormalities was done to ... the two pairs of cell lines obtained from 2 of 32 tumor fragments were extensively studied. They displayed similar morphology ... For example, chromosome number varied between 52 and 56 for CT320, between 49 and 55 for CT320X6, from 65 to 136 for CT329, and ... Tumor fragment F329 came from a patient who displayed a high response to FOLFOX treatment, but because of L-OHP-induced ...http://cancerres.aacrjournals.org/content/67/1/398
Positive predictive value of non-invasive prenatal screening for fetal chromosome disorders using cell-free DNA in maternal...
... for fetal chromosome abnormalities using cell-free deoxyribonucleic acid (cfDNA) in maternal serum has significantly influenced ... The fragments are most frequently 150-180 base pairs in length [1, 2] and derive mostly from apoptotic cells . During ... NIPS for fetal chromosome abnormalities using cfDNA in maternal serum is a powerful tool. It can be of enormous benefit to ... Non-invasive prenatal screening (NIPS) for fetal chromosome abnormalities using cell-free deoxyribonucleic acid (cfDNA) in ...https://bmcmedicine.biomedcentral.com/articles/10.1186/s12916-015-0374-8
Turner's syndrome - what is, facts, symptoms and treatment | Beltina.org
These abnormalities may include only a single X chromosome (X chromosome deletion) instead of the normal pair of X chromosomes ... or one complete and one fragmented or partial X chromosome. As well, the pattern may be mosaic, with some cells in the body ... A spontaneous (nonhereditary) chromosomal disorder in which there are abnormalities of the X CHROMOSOME, the SEX CHROMOSOME ... carrying the normal paired X chromosome complement and others carrying the abnormality. Turner's syndrome affects only females. ...https://www.beltina.org/turners-syndrome-what-is-facts-symptoms-and-treatment/
9q34 deletion syndrome - Wikipedia
The multicolour probes attach to a certain DNA fragment. MLPA is a test that finds and records DNA copy change numbers through ... The DNA samples (which are 25-80 base pairs in length) are then placed on slides to be observed under microscope. Lastly, EHMT1 ... Terminal deletions of chromosome 9q34 have been associated with childhood hypotonia, a distinctive facial appearance and ... MLPA can be used to detect tumours in the glial cells of the brain, as well as chromosomal abnormalities. Array-based ...https://en.wikipedia.org/wiki/9q34_deletion_syndrome
Prenatal Testing & Screening - What You Need To Know | BellyBelly
Close to 100 per cent of pregnant women choose to have an ultrasound to check for major physical abnormalities at 18 to 20 ... Close to 100 per cent of pregnant women choose to have an ultrasound to check for major physical abnormalities at 18 to 20 ... Chromosomal abnormalities are caused by defects in one of the 23 pairs of chromosomes that, deep inside our cells, carry all ... In CVS, a small fragment of placenta is removed with a syringe, either through the abdomen or via the vagina, at 10 to 12 weeks ...https://www.bellybelly.com.au/pregnancy/pre-natal-testing/
Genes & Chromosomes [M.Tevfik Dorak]
Chromosome abnormalities. Chromosome abnormalities may be numerical (aneuploidy: monosomy or trisomy) or structural: deletion, ... Telomeres have important functions such as preventing end-to-end fusion of chromosomes, assisting with chromosome pairing in ... fragments (May CA, 2002). The consequences of the obligatory recombination within PAR-1 are that genes show only partial sex ... Chromosomes differ in their sizes. The smallest human chromosome is chromosome 21 (50 Mb) and the largest one is chromosome 1 ( ...http://dorakmt.tripod.com/genetics/notes03.html
End-sequence profiling - Wikipedia
Chromosome abnormalities Chromosomal inversion Insertion (genetics) Deletion (genetics) Chromosomal translocation Chromosome ... Snyder and his group improved the ESP to 3kb resolution by sequencing both pairs of 3-kb DNA fragments without BAC construction ... the fragments is cloned into plasmids to construct artificial chromosome such as bacterial artificial chromosomes (BAC) which ... The size of fragments inserted is 150-350 kb. Another commonly used artificial chromosome is fosmid. The difference between BAC ...https://en.wikipedia.org/wiki/End-sequence_profiling
Glossary of Terms
Autosome Any chromosome that is not a sex chromosome; in humans there are 22 pairs of autosomes and one pair of sex chromosomes ... Chromosome abnormality Any variation from the normal chromosomal pattern. The common abnormalities found in Prader-Willi ... Translocation: a transfer of a fragment of one chromosome to another chromosome. ... Klinefelter's syndrome XXY syndrome A chromosome abnormality in which a male has an extra X chromosome. ...https://www.pwsausa.org/glossary-of-terms/
Mapping and Sequencing the Human Genome: Primer on Molecular Genetics
... or other identifiable DNA fragments are assigned to their respective chromosomes, with distances measured in base pairs. These ... associated with chromosomal abnormalities. cDNA map. A cDNA map shows the positions of expressed DNA regions (exons) relative ... which consist of fragments derived from source DNA enriched for a particular chromosome. (See Separating Chromosomes, above.) ... places on the same chromosome or on the other copy of the same chromosome (i.e., the homologous chromosome). This process ( ...https://biotech.law.lsu.edu/blaw/HGP/DOE1/prim2.html
COL18A1 - ddPCR Primer Pair - EvaGreen - Digital PCR | PrimePCR | Bio-Rad
The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal ... The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin a potent antiangiogenic protein. ... Chromosome Location:. 21:46896316-46897360question. Amplicon Length:. 85. Gene Information This gene encodes the alpha chain of ... Info: EG; Same primer pair as used in qPCR assay qHsaCID0012310; Intron-spanning ...http://www.bio-rad.com/en-us/prime-pcr-assays/assay/dhsaeg5018547-primepcr-ddpcr-expression-evagreen-assay-col18a1-human
Fluorescence in situ hybridization - Wikipedia
... of oligozoospermic men have an increased rate of sperm chromosome abnormalities. The analysis of chromosomes 21, X, and Y is ... These fragments are on the order of 100 thousand base-pairs, and are the basis for most FISH probes. Cells, circulating tumor ... In the eventual analysis, these fragments were put into order by digesting a copy of each fragment into still smaller fragments ... Each probe for the detection of mRNA and lncRNA is composed of 20 oligonucleotide pairs, each pair covering a space of 40-50 bp ...https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization
Microsoft word - parkinson-plus syndromes.doc
Tau protein abnormalities also have been found in frontotemporal dementia with parkinsonism linked to chromosome 17 ( FTDP-17 ... In Alzheimer disease, the pattern consists of a paired helical fragment triplet (55/64/69), whereas in PSP and CBGD a tau ... Abnormality of tau protein Of late, PSP has been considered 1 of the tau protein disorders. Cortical fibrillary tangles of PSP ... Abnormalities in peripheral nerves and muscles that are absent in patients with idiopathic PD have been found in patients with ...http://pharmpdf.com/m/multiplesystematrophy.info1.html
Array-CGH and multipoint FISH to decode complex chromosomal rearrangements | BMC Genomics | Full Text
Totally 53 chr3 fragments were identified having copy numbers from 0 to 14. MpFISH results from the BAC/PAC clones and array- ... Each copy number change on the array profile could be related to a specific chromosome aberration detected by metaphase mpFISH ... In most cases, the changes in copy number seen on array-CGH profiles reflected cumulative chromosome rearrangements. Most of ... for their ability to characterize complex rearrangements on human chromosome 3 (chr3) in tumour cell lines. We have used 179 ...https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-7-330
SIRT1 Deacetylates TopBP1 and Modulates Intra-S-Phase Checkpoint and DNA Replication Origin Firing
SIRT1-MT MEF cells displayed a high number of fragmented chromosomes, minute chromosomes, and chromosomes with quadro-crossover ... Three pairs of MEF cells at passage 2 were utilized. (C) Chromosome spreads showing abnormal chromosome structures (arrows) in ... 1C), the increased BrdU incorporation is unlikely to be due to greater cell proliferation, but rather abnormalities associated ... All the chromosome spreads were stained with Giemsa and chromosome number and structure were examined under a 100X lens with a ...http://www.ijbs.com/v10p1193.htm
Genome | Encyclopedia.com
The females have a single pair of chromosomes, while males have only a single chromosome. Like some other members of the insect ... Genetic abnormalities, where one chromosome is missing from the genome, can be represented in the same manner. For example, a ... A genomic library is a comprehensive collection of cloned DNA fragments derived from a genome. Each part of the genome is ... The H157:O7 chromosome is 20 percent larger, while MG1655 and H157:O7 share 4.1 million base pairs (mbp) in common. H157:O7 has ...https://www.encyclopedia.com/science-and-technology/biology-and-genetics/genetics-and-genetic-engineering/genome
Fundamentals of Human Genetics & Genomics | Current Medical Diagnosis & Treatment 2017 | AccessMedicine | McGraw-Hill Medical
One of these pairs, the sex chromosomes X and Y, determines the sex of the individual; females have the pair XX and males the ... The total chromosome count is determined first, followed by the sex chromosome complement and then by any abnormalities. The ... At the sites of breaks and new attachments of chromosome fragments, there may be permanent structural or functional damage to ... in which only one member of a pair of chromosomes is present; (2) trisomy, in which three chromosomes are present instead of ...https://accessmedicine.mhmedical.com/content.aspx?bookid=1843§ionid=135760835
Rhabdomyosarcoma | Cancer Genetics Web
A wide range of other chromosomal abnormalities have also been reported; particularly gain of material in chromosomes 2, 8, 12 ... To circumvent this problem, we irreversibly inverted the orientation of a 4.9 Mb syntenic fragment on chromosome 3, ... we applied a systematic method that takes into account germline and somatic alterations in 44 tumor-normal RMS pairs using deep ... Selected list of common recurrent structural abnormalities. Abnormality. Type. Gene(s). t(1;13)(p36;q14) in Rhabdomyosarcoma. ...http://www.cancerindex.org/geneweb/X2002.htm
DNA - New World Encyclopedia
For instance, the largest human chromosome, chromosome number 1, is 220 million base pairs long. ... This is because DNA will survive in the environment for less than one million years and slowly degrades into short fragments in ... Non-homologous recombination can be damaging to cells, as it can produce chromosomal translocations and genetic abnormalities. ... DNA usually occurs as linear chromosomes in eukaryotes, and circular chromosomes in prokaryotes. The set of chromosomes in a ...http://www.newworldencyclopedia.org/entry/DNA
Nutrition and the genome: a new chapter in health and disease: (EUFIC)
This DNA is arranged in 23 units or chromosomes, each chromosome occurs as a pair of 2 with one chromosome donated by each ... and shape of each chromosome type. The karyotype can be used to map gross abnormalities Messenger RNA (mRNA): RNA that serves ... The cDNAs with a sequence that matches the sequence of one of the gene fragments stick to the fragment like glue and the cDNA ... Chromosome: Structures that contain the genes. The human somatic cell has 23 pairs of chromosomes (46 chromosomes). ...http://www.eufic.org/en/healthy-living/article/nutrition-and-the-genome-a-new-chapter-in-health-and-disease/
Amplification of Genome-Representative DNA from Limited Sources with GenomePlex® WGA Technology for Use in Genetic Alterations...
The resulting DNA fragments are efficiently primed to generate a library of DNA fragments with defined 3' and 5' termini - the ... CMA uses CGH with bacterial artificial chromosome (BAC) or phage-derived artificial chromosome (PAC) clones (verified by ... producing a series of overlapping short templates ranging in the size from 200 to 1,500 base pairs and averaging 400 base pairs ... We combined GenomePlex WGA technology with a CGH array to detect chromosomal abnormalities in patients with mental and ...https://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/life-science-innovations/amplification-of-genome-representative.html
Protective Effects of Hydrogen against Low-Dose Long-Term Radiation-Induced Damage to the Behavioral Performances,...
... chromosome- and chromatid-type fragments, identified as acentric fragments that derived from a chromosome or chromatid ... Sperm Abnormality. Reproductive toxicity was tested by applying sperm-shape abnormalities assessment using the methods ... cut into 3-4 parts by a pair of clean scissors, and immediately placed into 1 mL PBS at 37°C. After standing for 20 minutes ... chromosome fragments, (e) chromosome with dicentrics and the accompanying fragments, (f) Robertsonian translocation, (g) ...https://www.hindawi.com/journals/omcl/2016/1947819/
Neither the New Zealand Genetically Hypertensive Strain nor Dahl Salt-Sensitive Strain Has an A1079T Transversion in the α1...
PCR fragments amplified from genomic DNA, with the use of the 1079+/1079− primer pair, and from control and mutant clones were ... Mapping of a quantitative trait locus for blood pressure on rat chromosome 2. J Clin Invest. 1994; 94: 431-436. ... However, it is still possible that some other, as yet undiscovered, abnormality in the untranscribed portion of the Atp1a1 gene ... The 1079+/1079− primer pair was chosen with the principal criteria of strong and specific amplification of a fragment that ...http://hyper.ahajournals.org/content/38/4/786
Molecular Genetic Testing in Pediatric Practice: A Subject Review | AMERICAN ACADEMY OF PEDIATRICS | Pediatrics
... from the maternally derived chromosome and the other originating from the paternally derived chromosome of each chromosome pair ... In addition to detecting large-scale deletions of chromosome material, FISH also can be used to detect abnormalities of ... Markers for DNA sequences in or near genes include restriction fragment length polymorphisms, variable number of tandem repeats ... A genetic locus is the place on homologous chromosome pairs where genes are located. Each gene is composed of 2 alternative ...http://pediatrics.aappublications.org/content/106/6/1494?ijkey=78adce5a88ab729c4dd697053eb9d8c267f9f9c5&keytype2=tf_ipsecsha
Hepatitis C Virus Inhibits DNA Damage Repair through Reactive Oxygen and Nitrogen Species and by Interfering with the ATM-NBS1...
B and C, SKY analysis of spontaneous chromosome abnormalities in HCV-infected JT and Raji cells. The t(8;14) translocation was ... We also examined the formation of MN, which are small nuclei arising from acentric chromatids or chromosome fragments, induced ... and complex rearrangements involving different pairs of chromosome (Fig. 2B). Similar observations were made in another cell ... 2F). The propensity for chromosome loss was greater than that for chromosome gain (Fig. 2F, 2G). As cancer cell lines, such as ...http://www.jimmunol.org/content/185/11/6985.long