MARIAN ELLIOTT KOSHLAND | Biographical Memoirs: Volume 90 | The National Academies Press
T T K O S H L A N D MARIAN ELLIO 233 1967 Location of specificity and allotypic amino acid residues in antibody Fd fragments. ... quisitely careful amino acid composition analyses, convincingly showed that these antibodies had different amino acid ... Differences in amino acid composition related to allotypic and antibody specificity of rabbit heavy chains. Immunochemistry 5: ... Differences in amino acid composition of two purified antibodies from the same rabbit. Proc. Natl. Acad. Sci. U. S. A. 50:61-68 ...https://www.nap.edu/read/12562/chapter/11
Recombinant Human Antibodies Against Aldehyde-Modified Apolipoprotein B-100 Peptide Sequences Inhibit Atherosclerosis |...
... amino acids 661 to 680) or P210 (KTTKQSFDLSVKAQYKKNKH, amino acids 3136 to 3155) were selected from the single-chain fragment- ... A total of 4 scFv with specificity for the MDA-modified apoB-100 peptide composed of amino acids 661 to 680 (IEI-A8, IEI-D8, ... 11 Single-chain human antibody fragments with specificity for MDA-modified apoB-100-derived peptides P45 (IEIGLEGKGFEPTLEALFGK ... P2 (amino acids [aa] 16 to 31, red), P45 (aa 661 to 680, blue), P143 (aa 2131 to 2150, orange), P210 (aa 3136 to 3155, purple ...http://circ.ahajournals.org/content/110/14/2047
A recombinant fragment of rabphilin [amino acid (aa) 1-361] was expressed and purified from bacteria in its wild-type form (WT ... A large proportion of rabphilin is phosphorylated on S234 after a 2 min high K+ stimulation.A, Specificity of the antibody ... Briefly, a peptide corresponding to amino acids 230-239 (TRRASEARMS) of rabphilin was synthesized with an additional cysteine ... Equal amounts of rabphilin wild-type fragment (WT), wild-type fragment phosphorylated by PKA (WT/PKA), and each of the two ...http://www.jneurosci.org/content/21/15/5473
Lamin B Receptor Antibody (BB2SS3F3) [Alexa Fluor® 700] (NBP2-59947AF700): Novus Biologicals
Mouse Monoclonal Anti-Lamin B Receptor Antibody (BB2SS3F3) [Alexa Fluor® 700]. Validated: WB, ICC/IF, IHC, IHC-P. Tested ... GST fused to N-terminal fragment of human LBR (amino acids residues 1-211) ... Specificity. Specificity of human, mouse Lamin B Receptor antibody verified on a Protein Array containing target protein plus ... Reviews for Lamin B Receptor Antibody (NBP2-59947AF700) (0) There are no reviews for Lamin B Receptor Antibody (NBP2-59947AF700 ...https://www.novusbio.com/products/lamin-b-receptor-antibody-bb2ss3f3_nbp2-59947af700
PSMA/FOLH1/NAALADase I Antibody (GCP-04) (NBP1-45057): Novus Biologicals
Mouse Monoclonal Anti-PSMA/FOLH1/NAALADase I Antibody (GCP-04). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, ... PSMA/FOLH1/NAALADase I Antibody (GCP-04) Summary. Immunogen. Recombinant fragment of human GCPII (amino acids 44-750) produced ... Specificity. The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate ... Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Fragment of human GCPII (aminoacids 44-750) produced in ...https://www.novusbio.com/products/psma-folh1-naaladase-i-antibody-gcp-04_nbp1-45057
Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and...
Anti-Mcm2 monoclonal antibody (mAb) (mouse IgG1 isotype) was raised against a fragment of human Mcm2 (amino acids 725-888; BM28 ... Specificity of anti-Mcm2 mAb and anti-Mcm5 polyclonal antibody was established by immunoblot, immunofluorescence, and ... Rabbit polyclonal antibodies were raised against a fragment of human Mcm5 (amino acids 372-590) and purified by affinity ... Immunostaining with the Mcm2 antibody yielded predominantly nuclear staining. The Mcm5 antibody stained nuclei but also cell ...http://gut.bmj.com/content/50/3/373
Monoclonal antibody therapy - Wikipedia
... which consist of three antibody hypervariable amino acid domains responsible for the antibody specificity embedded into ... fragment-antigen binding) part can be separated from the Fc (fragment constant) part of the molecule. The Fab fragments contain ... Humanised antibodies are produced by grafting murine hypervariable regions on amino acid domains into human antibodies. This ... Immunoliposomes have been successfully used in vivo to convey tumour-suppressing genes into tumours, using an antibody fragment ...https://en.wikipedia.org/wiki/Monoclonal_antibody_therapy
epithelial Sodium Channel gamma Antibody (11-35-1) - BSA Free (NBP2-41374): Novus Biologicals
Mouse Monoclonal Anti-epithelial Sodium Channel gamma Antibody (11-35-1) - BSA Free. Validated: WB, ELISA, ICC/IF, IHC, IHC-Fr ... neoepitope generated after proteolytic cleavage of human gENaC and recognizes the C-terminal fragment starting from amino acid ... Specificity. Antibody is specific for the ... Secondary Antibodies. *Anti-Mouse HRP Labeled Antibodies. *Anti ... Home » epithelial Sodium Channel gamma » epithelial Sodium Channel gamma Antibodies » epithelial Sodium Channel gamma Antibody ...https://www.novusbio.com/products/epithelial-sodium-channel-gamma-antibody-11-35-1_nbp2-41374
Molecular basis of β-amyloid oligomer recognition with a conformational antibody fragment | PNAS
To analyze the molecular basis of its oligomeric specificity, we turned to the KW1 amino acid sequence and determined the ... Such antibodies and antibody fragments have been reported to occur with different specificities, and considerable applications ... which are responsible for the molecular specificity of the antibody fragment, differ profoundly from those of fibril-specific ... Aβ oligomer recognition with an antibody fragment. Isabel Morgado, Karin Wieligmann, Magdalena Bereza, Raik Rönicke, Katrin ...http://www.pnas.org/content/109/31/12503?ijkey=7e80de8a362583404e36a225aacba2204f0da0b6&keytype2=tf_ipsecsha
A rapid and scalable method for selecting recombinant mouse monoclonal antibodies | BMC Biology | Full Text
Here we report a rapid and scalable method to select and express recombinant mouse monoclonal antibodies that are essentially ... This method can be used to quickly select several high quality monoclonal antibodies from a single immunized mouse and ... we selected antibodies that gave expected staining patterns on wholemount fixed zebrafish embryos. ... Monoclonal antibodies with high affinity and selectivity that work on wholemount fixed tissues are valuable reagents to the ...https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-8-76
Osteocalcin Antibody (8H12F9H10) [DyLight 680] (NB100-62805FR): Novus Biologicals
Mouse Monoclonal Anti-Osteocalcin Antibody (8H12F9H10) [DyLight 680]. Validated: ELISA. Tested Reactivity: Human. 100% ... It does not recognize fragments representing amino acids 20-43 or intact bovine osteocalcin.. Osteocalcin (Bone Gla Protein) is ... Specificity. This product recognizes intact human and bovine osteocalcin in a "2 site" assay and reacts with osteocalcin ... fragments corresponding to amino acids 1-19, 7-19 and 15-31 of the native molecule. ...https://www.novusbio.com/products/osteocalcin-antibody-8h12f9h10_nb100-62805fr
US20110081408A1 - Anti-chemokine and associated receptors antibodies for inhibition of growth of neoplasms - Google...
... the particular chemokines which are over-expressed in the tumor using methods of the invention and administer antibodies ... invention provides a means of inhibiting the growth and metastasis of cancer cells by administering anti-chemokine antibodies. ... that consists of 10 to 15 amino acids from Sequences 1 through 22. The antibodies or antigen-binding fragment(s) can be ... Antibodies or antigen-binding fragments with specificity for functional mutant or variant mammalian chemokines are appropriate ...https://patents.google.com/patent/US20110081408A1/en
US20160244518A1 - Methods for prevention and treatment of inflammation using anti-chemokine antibodies - Google Patents
Identification of chemokines which are over-produced makes it possible to block specific chemokine activity using antibodies to ... It is possible to inhibit inflammatory processes by administration of antibodies to chemokines. ... The antibodies or antigen-binding fragment(s) bind to epitope(s) or peptide(s) that consists of 10 to 15 amino acids from ... Antibodies or antigen-binding fragments with specificity for functional mutant or variant mammalian chemokines are appropriate ...https://patents.google.com/patent/US20160244518A1/en?oq=US+14%2F843%2C764
CASQ1 / Calsequestrin 1 Antibody for WB/Western LS-C155368
Calsequestrin 1 antibody LS-C155368 is an unconjugated rabbit polyclonal antibody to Calsequestrin 1 (CASQ1) from human, mouse ... Recombinant fragment corresponding to a region within amino acids 136 and 381 of Calsequestrin 1 (SwissProt P31415). Percent ... Specificity. Human, mouse, rat CASQ1. Usage. Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet ... Calsequestrin 1 antibody LS-C155368 is an unconjugated rabbit polyclonal antibody to Calsequestrin 1 (CASQ1) from human, mouse ...https://www.lsbio.com/antibodies/casq1-antibody-calsequestrin-1-antibody-wb-western-ls-c155368/162211
Improved scFv Anti-HIV-1 p17 Binding Affinity Guided from the Theoretical Calculation of Pairwise Decomposition Energies and...
The new antibodies were designed by mutating the potential amino acid residues in CDRs of scFv anti-p17. With the guide from ... Single chain Fv fragments display the binding specificity, monovalent binding affinity of full-size antibodies, and provide the ... Potential amino acids that involved changing nonalanine amino acids into alanines will be listed up. The relevant change of ... One type of genetically engineered antibody is the single chain Fv fragment (scFv). Single chain Fv fragments are genetically ...https://www.hindawi.com/journals/bmri/2013/713585/
KR20140033986A - Protein complex for preparing bispecific antibodies and method using thereof - Google Patents
The present invention relates to protein complexes and a method of manufacturing bispecific antibodies using protein complexes ... C L comprises an amino acid sequence having sufficient variable region sequence to impart specificity to the antigen It is ... Multispecific antibodies comprising full length antibodies and single chain fab fragments WO2002044215A2 (en) 2002-06-06. ... wherein the cleavable amino acid sequence is SEQ ID NO-protein conjugate having an amino acid sequence selected from the group ...https://patents.google.com/patent/KR20140033986A/en
US Patent # 9,770,011. Veterinary composition and methods for non-surgical neutering and castration - Patents.com
The term "donor antibody" refers to an antibody (e.g., monoclonal or recombinant) which contributes the amino acid sequences of ... which retains the same antigen binding specificity and/or neutralizing ability as the antibody from which the fragment was ... pyroglutamic acid; # = amide; Underlined amino acid differ from GnRH-I amino acid sequence. GnRH has the same amino acid ... Complete canine antibody heavy chain amino acid sequence (variable heavy sequence (SEQ ID NO: 14) fused to antibody constant ...http://patents.com/us-9770011.html
GENE RECOMBINANT ANTIBODY AND ANTIBODY FRAGMENT THEREOF - Patent application
... and the amino acid sequence of the CDR regulates binding reactivity and binding specificity of the antibody (J. Exp. Med., 132 ... in the amino acid sequence represented by SEQ ID NO:16. Antibodies and antibody fragments in which one or more amino acids are ... 0124] aspartic acid, glutamic acid, isoaspartic acid, isoglutamic acid, 2-aminoadipic acid, 2-aminosuberic acid; Group C: [0125 ... has the amino acid sequence of human antibody κ class.  A human CDR-grafted antibody is an antibody in which CDR amino ...http://www.patentsencyclopedia.com/app/20120276090
Patent US6372957 - Transgenic mouse comprising a MEF2 binding site operatively linked to an ... - Google Patents
A HDAC5 deletion mutant lacking amino acids 22-488 (HDAC5-ΔN), including the 18-amino acid minimal MEF2-interactin region, ... Single-chain antibody variable fragments (Fvs) in which the C-terminus of one variable domain is tethered to the N-terminus of ... Immunohistological analysis for the expression of a transgene by using an antibody of appropriate specificity can be performed ... Once the component amino acids of the turn are determined, mimetics can be constructed to achieve a similar spatial orientation ...http://www.google.com/patents/US6372957?dq=7125605
Type-specific immunogenicity of a chemically synthesized peptide fragment of type 5 streptococcal M protein. | JEM
The synthetic peptides, designated S-M5(1-20) and S-M5(20-40), represent the amino-terminal amino acid sequence of the native ... antibodies in rabbits without evoking heart cross-reactive antibodies. ... We determined the antigenic specificity and protective immunogenicity of two chemically synthesized peptides of type 5 ... Type-specific immunogenicity of a chemically synthesized peptide fragment of type 5 streptococcal M protein.. J B Dale, J M ...http://jem.rupress.org/content/158/5/1727
Suppression of MMP activity in bovine cartilage explants cultures has little if any effect on the release of aggrecanase...
Fragments released into the supernatant were determined using a range of neo-epitope specific immunoassays; (1) sandwich ... We found that (1) aggrecanase-derived aggrecan fragments are released in the early (day 2-7) and mid phase (day 9-14) into the ... We therefore investigated the time-dependant release ex vivo of MMP- and aggrecanase-derived fragments of aggrecan and type II ... the MMP-derived aggrecan and type II collagen fragments were released in the late phase (day 16-21) only. Our data support the ...https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-259
Anti-VDAC1 / PORIN Antibody (aa1-211) for IHC, WB/Western LS-C186351
PORIN antibody LS-C186351 is an immunoaffinity-purified rabbit polyclonal that binds aa1-211 of human PORIN (also known as ... VDAC1 / PORIN antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 211 of VDAC1 ... Specificity:. Human PORIN / VDAC-1. Usage:. IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer ... VDAC1 Antibody, HVDAC1 Antibody, Plasmalemmal porin Antibody, Porin 31HM Antibody, PORIN Antibody, PORIN-31-HL Antibody, Vdac5 ...https://www.lsbio.com/antibodies/anti-vdac1-antibody-porin-antibody-aa1-211-ihc-wb-western-ls-c186351/194003
NOVEL CHEMICAL COMPUNDS AND THEIR USE - BIOTA SCIENTIFIC MANAGEMENT PTY LTD
... wherein the fragments retain the binding specificity of the whole antibody molecule. The binding fragments include, for example ... 4) Suzuki, H.; Kajimoto, Y.; Kumagai, H. Improvement of the Bitter Taste of Amino Acids through the Transpeptidation Reaction ... Also included for moiety X are antibodies or antigen binding fragments of whole antibody, ... introduction of a glucuronic acid residue or a lipophile-modified version of glucuronic acid HO OH UDP-glucuronic acid 0 ooh ...http://www.sumobrain.com/patents/wipo/Novel-chemical-compunds-their-use/WO2004050677.html
US20080292618A1 - Intranuclear protein transduction through a nucleoside salvage pathway - Google Patents
... antibody mAb 3E10, and variants and/or functional fragments thereof. The nucleotide and amino acid sequences for the variable ... As used herein, the phrase "functional fragments of an antibody having the binding specificity of the antibody as produced by ... The free amino group (at the amino terminus of a protein or on a lysine) in amino acid residues may be reacted with a compound ... Thus, in certain embodiments, a functional fragment of an antibody having the binding specificity of the antibody as produced ...https://patents.google.com/patent/US20080292618A1/en
Characterization of the Borna disease virus phosphoprotein, p2...
Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with t ... Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments ... and synthetic peptides were analyzed to characterize the specificities of antibodies to p23. Epitope mapping revealed eight ... None of these epitopes was reactive with antibodies to p40. Cross-reactivity with monospecific sera and monoclonal antibodies ...https://www.mysciencework.com/publication/show/characterization-of-the-borna-disease-virus-phosphoprotein-p23