Human Metabolome Database: Showing metabocard for Chenodeoxycholoyl-CoA (HMDB0006292)
... specific amidation activity of both chenodeoxycholoyl-CoA is always found in the most peroxisome-rich subcellular fractions. ( ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in catalytic activity. Specific function:. Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze ...http://www.hmdb.ca/metabolites/HMDB0006292
Pharmacology-Lithium's Effects on Cyclic AMP, Membrane Transport, and Cholinergic Mechanisms | SpringerLink
These enzymes have been found to vary in activity in specific regions of the brain (Weiss and Costa, 1968). Adenyl cyclase has ... and to be associated with subcellular fractions that contain membrane fragments and nerve endings (De Robertis et al., 1967). ... Forn, J., and Valdecasas, F. G., 1971, Effects of lithium on brain adenyl cyclasc activity, Biochem. Pharmacol. 20:2773.PubMed ... Adenyl Cyclase Adenyl Cyclase Activity Acetylcholine Release Cholinergic Mechanism Affective Illness These keywords were added ...https://link.springer.com/chapter/10.1007/978-1-4684-2022-7_6
Inositol 1,4,5-trisphosphate turnover enzymes--activities and subcellular distribution in hepatocarcinogenesis. :...
The total phosphatase activity was found to be distributed between cytosol (15%) and membraneous fractions (75%), with most of ... The total phosphatase activity was found to be distributed between cytosol.... The metabolism of inositol 1,4,5-trisphosphate ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ...http://oxfordindex.oup.com/view/10.1093/carcin/18.12.2447
Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Action of the hormone...
... the incorporation of precursor into RNA in all subcellular fractions other than the nuclear one, restores incorporation of ... After treatment with noradrenaline the increase in radioactivity was found solely in AMP after hydrolysis of microsomal RNA to ... decarboxylase activities and causes a 12-fold increase in cytoplasmic Mn2+-dependent polyadenylate polymerase activity. ... rabbit heart with reserpine causes a decrease of incorporation of labelled precursors into RNA species of subcellular fractions ...http://www.biochemj.org/content/168/3/341
Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex and hypothalamus in...
It was found out that Al-AT activities under impairment of feeding regime depend on several factors including morphofunctional ... On the level of mitochondrial fractions, the enzyme is involved into the complex of compensatory reactions via glucose-alanin ... mitochondrial and cytosol fractions of visual, orbital, motor, limbic areas of brain cortex and hypothalamus of three-month old ... Total and specific activities of alaninaminotransferase (Al-AT) were determined in general tissues, ...http://ijpr.sbmu.ac.ir/article_99.html
Existence of a reverse transcriptase-p30 complex in AKR mice with a high incidence of spontaneous lymphocytic leukaemia. -...
Significant levels of RT activity were first detected in the microsomal fractions of the two tissues at 15 and 20 weeks old, ... Although low amounts of p30 could be found in both tissues within the first week of life, the overall increase in the amount of ... In addition, a protein complex consisting of p30 and RT was first found in thymus and spleen lymphocytes of 15 and 20 week-old ... The complex could not be found in lymphocytic tissue taken from younger animals or in 'non-target' organs, such as liver or ...https://www.semanticscholar.org/paper/Existence-of-a-reverse-transcriptase-p30-complex-in-Bandyopadhyay-Fish/f638187057b3967c0f3de6c6b931e918b5d1baf3
Freshly isolated hPT cells exhibited high levels of GSH-dependent enzyme activities, and subcellular fractions prepared from ... In other studies (Lasker et al., 2000), we found renal CYP4A11 to be localized exclusively in the S2 and S3 segments of the ... 5 times higher than hexokinase activity at passage 4. GGT activity remained relatively constant through passage 4. GRD activity ... Activities of GSH-Dependent Enzymes in Freshly Isolated hPT Cells.. Activities of GGT, GCS, GST, GRD, GPX, and hexokinase were ...http://jpet.aspetjournals.org/content/293/2/677
Protocols and Video Articles Authored by Lei Cao (Translated to Japanese)
The wild type form of RAI1 was found in the chromatin bound and nuclear matrix subcellular fractions while the mutant product ... We found this effect in melanoma and colon cancer models, and that it was not caused by physical activity alone. Serum from ... Disease activity was measured using the Crohn's Disease Activity Index (CDAI). Regression analyses of REE with CDAI score, C- ... subcellular localization and transcriptional activity. Clinical features of patients with or without 17p11.2 deletions and ...https://www.jove.com/author/Lei_Cao?language=Japanese
AGPAT enzyme activity could not be demonstrated, and instead the protein was found to have GPAT activity with NEM sensitivity ( ... 2). Evidence for GPAT3 localization to the ER includes immunofluorescence and Western blots of subcellular fractions (17, 113 ... Lipin-1 PAP activity is modulated by factors that influence its subcellular localization. Prior to the identification of the ... GPAT2 activity was first identified as the mitochondrial GPAT activity remaining in Gpat1−/− mice (73). In contrast to GPAT1, ...http://ajpendo.physiology.org/content/296/6/E1195
... enzyme activity with enzyme content data compared to those extrapolated from the data obtained from subcellular fractions or ... 2001) Non-synonymous single nucleotide alterations found in the CYP2C8 gene result in reduced in vitro paclitaxel metabolism. ... This suggests that, if any developmental changes in CYP2C8 and CYP1A2 activity occur after about 35 days and 15 months ... 2013) Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic ...http://dmd.aspetjournals.org/content/45/5/468
In addition, we found that the increase in PDE3 activity was limited to the subcellular fraction expressing only PDE3B, and not ... Subcellular fractionation revealed that cp/cp VSMC particulate PDE3 activity was increased relative to +/?-derived VSMCs. ... PDE3 and PDE4 activities in JCR:LA-cp rat aortae.. Aortic cAMP PDE activity of the JCR:LA-cp rat strain was predominantly made ... When PDE3 and PDE4 activities were measured in isolated cytosolic and particulate fractions of aortic tissue or of VSMCs, ...http://diabetes.diabetesjournals.org/content/51/4/1194
... this activity is measured in a test tube under optimal conditions using a subcellular fraction as the enzyme source. Without ... We found that any precursor that will directly generate a steady stream of methylselenol is more active than selenite or ... As shown in Table 6 ⇓ , selenium deficiency caused a marked drop in the activities of both enzymes. Sodium selenite was used as ... Ip C., Ganther H. E. Activity of methylated forms of selenium in cancer prevention. Cancer Res., 50: 1206-1211, 1990. ...http://cancerres.aacrjournals.org/content/60/11/2882
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... Proton and calcium transport activities in the fraction were characterized by fluorescence microscopy and spectrophotometric ... as we can now isolate highly purified fractions that could be used for proteomic analysis to find proteins that may clarify the ... We report the purification and characterization of an acidocalcisome fraction from T. gondii tachyzoites after subcellular ...https://www.jove.com/visualize/abstract/21541023/calcium-uptake-proton-transport-acidocalcisomes-toxoplasma
Differential Effects of B Cell Receptor and B Cell Receptor-FcγRIIB1 Engagement on Docking of Csk to GTPase-activating Protein ...
... this complex is found in subcellular membrane or cytoskeletal fractions. Thus, GAP.A-p62 may represent a docking protein for ... 1991) Protein-tyrosine kinases regulate the phosphorylation, protein interactions, subcellular distribution, and activity of ... only a fraction of total cellular Src-related kinase activity is present in such complexes and negatively regulated by Csk. The ... exhibit increased activity of Src and related kinases ((19), (20)). In response to extracellular signals, the activity of Csk ...http://jem.rupress.org/content/186/2/259
The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver |...
... and no endogenous activity was detected in either purified Golgi apparatus or plasma membrane fractions. Limited protease ... we have examined the site and topology of dihydroceramide synthesis in well-characterized subcellular fractions from rat liver ... The distribution of dihydroceramide synthesis was found to exactly parallel that of an endoplasmic reticulum (ER) marker upon ...http://www.biochemj.org/content/290/3/751
CrkRS | Journal of Cell Science
In subcellular fractionation studies the bulk of CrkRS remains in the insoluble fraction of the nucleus, even after nuclease ... We then assayed whether we were able to detect kinase activity in anti-CrkRS immunoprecipitates. We found that anti-CrkRS ... We found that α -amanitin caused similar changes in the immunofluorescence pattern with both SC35 and anti-CrkRS antibodies ( ... We found that anti-CrkRS antibodies exclusively stained the nucleus. Furthermore, CrkRS appeared to be localized in a discrete ...http://jcs.biologists.org/content/114/14/2591
A study of the glycerol phosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activities in rat liver...
The subcellular distribution of this enzyme differed from that of GPAT. The highest specific activity of DHAPAT was found in a ... This fraction also contained the highest specific activity of the peroxisomal marker uricase. DHAPAT activity in mitochondrial ... activities were measured both in subcellular fractions prepared from fed rat liver and in whole homogenates prepared from ... The GPAT and DHAPAT activities in mitochondrial fractions had a considerably higher affinity for the palmitoyl-CoA substrate. 6 ...http://www.biochemj.org/content/182/3/751
Valosin-containing protein - Wikipedia
A fraction of p97/CDC48 was also found in the nucleus. According to the crystal structures of full-length wild-type p97, six ... Mutations in p97 can also influence its activity. For example, p97 mutant proteins carrying single point mutations found in ... The subcellular localization of CDC48 has not been fully characterized, but is likely to be similar to the mammalian ... D2 mediates the major enzyme activity, and D1 contributes to the heat-induced activity". The Journal of Biological Chemistry. ...https://en.wikipedia.org/wiki/Valosin-containing_protein
Protocols and Video Articles Authored by Vladislav Levchenko (Translated to Italian)
... we found that betaPix does not decrease ENaC activity through its guanine nucleotide exchange factor (GEF) activity for Rac1 ... 14C]7alpha-OOH radioactivity accumulated much faster in SC2A mitochondria than in VC, whereas other subcellular fractions ... We found that Rac1 inhibitor NSC23766 markedly decreased ENaC activity in freshly isolated collecting ducts. Knockdown of Rac1 ... We have shown recently that Rac1 increases ENaC activity, whereas Cdc42 fails to change channel activity. Here we tested ...https://www.jove.com/author/Vladislav_Levchenko?language=Italian
Frontiers | A Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome | Plant Science
The method is based on the separation of cellular fractions via density gradients consisting of organic, non-aqueous solvents. ... The calculation algorithm performs correlation of marker enzyme activity and metabolite abundance accounting for technical ... The method is based on the separation of cellular fractions via density gradients consisting of organic, non-aqueous solvents. ... The presented benchtop method aims at unravelling subcellular metabolome dynamics in a precise and statistically robust manner ...https://www.frontiersin.org/articles/10.3389/fpls.2016.01912/full
Compositions and methods relating to novel compounds and targets thereof - Glick, Gary D.
It was found in these past studies that in addition to cytotoxic activity, Bz-423 was also cytostatic under some conditions. ... Neither Bz-423 nor antimycin A generated substantial ROS in the subcellular S15 fraction (cytosol and microsomes), and Bz-423 ... The patches are usually found on the arms, legs, trunk, or scalp but may be found on any part of the skin. The most typical ... The compounds show maximum activity at high substrate concentration and minimal activity (e.g., F1Fo-ATPase inhibiting) at low ...http://www.freepatentsonline.com/y2006/0025388.html
Epidemic dropsy - Wikipedia
... are involved in AO induced toxicity causing peroxidative damage of lipids in various hepatic sub-cellular fractions including ... Alkaloid content in argemone oil varies from 0.44% to 0.50%. Argemone seeds find use as a substitute because of the easy ... Cardiac Failure:The inhibition of Na+-K+-ATPase activity of heart by sanguinarine is due to interaction with the cardiac ... The damage in hepatic microsomal membrane causes loss of activity of cytochrome P-450 and other membrane bound enzymes ...https://en.wikipedia.org/wiki/Epidemic_dropsy
Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates |...
Guilluy, G., Dubash, A. D., García-Mata, R. Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature ... The presented GEF assay was recently modified for application for nuclear fractions 9,10. With further modifications, testing ... find the optimal cell number, lysis buffer volume, and detection method for the GEF to be tested (a good antibody for Western ... of GEF activation in other subcellular compartments might also be possible.. We use the presented method to study activation of ...https://www.jove.com/video/3932/affinity-precipitation-active-rho-gefs-using-gst-tagged-mutant-rho
Compositions relating to novel compounds and targets thereof - The Regents of the University of Michigan
Neither Bz-423 nor antimycin A generated substantial ROS in the subcellular S15 fraction (cytosol and microsomes), and Bz-423 ... Effect of Bz-423 on ODC Levels and Activity  To determine whether ODC activity and polyamine metabolism is affected by Bz ... The present invention provides novel compounds that find use in treating a number of diseases and conditions and that find use ... the compounds of the present invention do not generate substantial ROS in the subcellular S15 fraction (e.g., cytosol; ...http://www.freepatentsonline.com/y2004/0220180.html
Phosphodiesterase 4D acts downstream of Neuropilin to control Hedgehog signal transduction and the growth of medulloblastoma |...
In our study, we found that Sema3 signals through Nrps to promote PDE4D activity and inhibit PKA, which ultimately potentiates ... Alternatively, PKA may control Gli2 and Gli3 at distinct subcellular locations, and Sema3-Nrp only modulates PKA activity where ... The pellet was designated as the membrane fraction, and was resuspended in RIPA buffer. For each fraction, the protein ... The cilium or its base appears to be a key site for regulated AC activity and PKA activity regarding Gli processing and likely ...https://elifesciences.org/articles/07068