Human Metabolome Database: Showing metabocard for Chenodeoxycholoyl-CoA (HMDB0006292)
... specific amidation activity of both chenodeoxycholoyl-CoA is always found in the most peroxisome-rich subcellular fractions. ( ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in catalytic activity. Specific function:. Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze ...http://www.hmdb.ca/metabolites/HMDB0006292
Inositol 1,4,5-trisphosphate turnover enzymes--activities and subcellular distribution in hepatocarcinogenesis. :...
The total phosphatase activity was found to be distributed between cytosol (15%) and membraneous fractions (75%), with most of ... The total phosphatase activity was found to be distributed between cytosol.... The metabolism of inositol 1,4,5-trisphosphate ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ...http://oxfordindex.oup.com/view/10.1093/carcin/18.12.2447
Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Action of the hormone...
... the incorporation of precursor into RNA in all subcellular fractions other than the nuclear one, restores incorporation of ... After treatment with noradrenaline the increase in radioactivity was found solely in AMP after hydrolysis of microsomal RNA to ... decarboxylase activities and causes a 12-fold increase in cytoplasmic Mn2+-dependent polyadenylate polymerase activity. ... rabbit heart with reserpine causes a decrease of incorporation of labelled precursors into RNA species of subcellular fractions ...http://www.biochemj.org/content/168/3/341
Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex and hypothalamus in...
It was found out that Al-AT activities under impairment of feeding regime depend on several factors including morphofunctional ... On the level of mitochondrial fractions, the enzyme is involved into the complex of compensatory reactions via glucose-alanin ... mitochondrial and cytosol fractions of visual, orbital, motor, limbic areas of brain cortex and hypothalamus of three-month old ... Total and specific activities of alaninaminotransferase (Al-AT) were determined in general tissues, ...http://ijpr.sbmu.ac.ir/article_99.html
Freshly isolated hPT cells exhibited high levels of GSH-dependent enzyme activities, and subcellular fractions prepared from ... In other studies (Lasker et al., 2000), we found renal CYP4A11 to be localized exclusively in the S2 and S3 segments of the ... 5 times higher than hexokinase activity at passage 4. GGT activity remained relatively constant through passage 4. GRD activity ... Activities of GSH-Dependent Enzymes in Freshly Isolated hPT Cells.. Activities of GGT, GCS, GST, GRD, GPX, and hexokinase were ...http://jpet.aspetjournals.org/content/293/2/677
AGPAT enzyme activity could not be demonstrated, and instead the protein was found to have GPAT activity with NEM sensitivity ( ... 2). Evidence for GPAT3 localization to the ER includes immunofluorescence and Western blots of subcellular fractions (17, 113 ... Lipin-1 PAP activity is modulated by factors that influence its subcellular localization. Prior to the identification of the ... GPAT2 activity was first identified as the mitochondrial GPAT activity remaining in Gpat1−/− mice (73). In contrast to GPAT1, ...http://ajpendo.physiology.org/content/296/6/E1195
Protocols and Video Articles Authored by Lei Cao (Translated to Spanish)
The wild type form of RAI1 was found in the chromatin bound and nuclear matrix subcellular fractions while the mutant product ... We found this effect in melanoma and colon cancer models, and that it was not caused by physical activity alone. Serum from ... Disease activity was measured using the Crohn's Disease Activity Index (CDAI). Regression analyses of REE with CDAI score, C- ... subcellular localization and transcriptional activity. Clinical features of patients with or without 17p11.2 deletions and ...https://www.jove.com/author/Lei_Cao?language=Spanish
In addition, we found that the increase in PDE3 activity was limited to the subcellular fraction expressing only PDE3B, and not ... Subcellular fractionation revealed that cp/cp VSMC particulate PDE3 activity was increased relative to +/?-derived VSMCs. ... PDE3 and PDE4 activities in JCR:LA-cp rat aortae.. Aortic cAMP PDE activity of the JCR:LA-cp rat strain was predominantly made ... When PDE3 and PDE4 activities were measured in isolated cytosolic and particulate fractions of aortic tissue or of VSMCs, ...http://diabetes.diabetesjournals.org/content/51/4/1194
... this activity is measured in a test tube under optimal conditions using a subcellular fraction as the enzyme source. Without ... We found that any precursor that will directly generate a steady stream of methylselenol is more active than selenite or ... As shown in Table 6 ⇓ , selenium deficiency caused a marked drop in the activities of both enzymes. Sodium selenite was used as ... Ip C., Ganther H. E. Activity of methylated forms of selenium in cancer prevention. Cancer Res., 50: 1206-1211, 1990. ...http://cancerres.aacrjournals.org/content/60/11/2882
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... Proton and calcium transport activities in the fraction were characterized by fluorescence microscopy and spectrophotometric ... as we can now isolate highly purified fractions that could be used for proteomic analysis to find proteins that may clarify the ... We report the purification and characterization of an acidocalcisome fraction from T. gondii tachyzoites after subcellular ...https://www.jove.com/visualize/abstract/21541023/calcium-uptake-proton-transport-acidocalcisomes-toxoplasma
Differential Effects of B Cell Receptor and B Cell Receptor-FcγRIIB1 Engagement on Docking of Csk to GTPase-activating Protein ...
... this complex is found in subcellular membrane or cytoskeletal fractions. Thus, GAP.A-p62 may represent a docking protein for ... 1991) Protein-tyrosine kinases regulate the phosphorylation, protein interactions, subcellular distribution, and activity of ... only a fraction of total cellular Src-related kinase activity is present in such complexes and negatively regulated by Csk. The ... exhibit increased activity of Src and related kinases ((19), (20)). In response to extracellular signals, the activity of Csk ...http://jem.rupress.org/content/186/2/259
A study of the glycerol phosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activities in rat liver...
The subcellular distribution of this enzyme differed from that of GPAT. The highest specific activity of DHAPAT was found in a ... This fraction also contained the highest specific activity of the peroxisomal marker uricase. DHAPAT activity in mitochondrial ... activities were measured both in subcellular fractions prepared from fed rat liver and in whole homogenates prepared from ... The GPAT and DHAPAT activities in mitochondrial fractions had a considerably higher affinity for the palmitoyl-CoA substrate. 6 ...http://www.biochemj.org/content/182/3/751
The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver |...
... and no endogenous activity was detected in either purified Golgi apparatus or plasma membrane fractions. Limited protease ... we have examined the site and topology of dihydroceramide synthesis in well-characterized subcellular fractions from rat liver ... The distribution of dihydroceramide synthesis was found to exactly parallel that of an endoplasmic reticulum (ER) marker upon ...http://www.biochemj.org/content/290/3/751
In untreated control cells, most of this activity found with the nuclear fraction can be extracted by chelators. Phorbol easter ... These results suggest that tumor promoters may induce association (activation) of protein kinase C with different subcellular ... of protein kinase C to the particulate fraction. Part of the enzyme activity stabilized to the membrane fraction in response to ... An apparently pure nuclear fraction prepared from NIH 3T3 cells was found to contain 25-30% of the total membrane-associated ...http://cancerres.aacrjournals.org/content/48/7/1910
Dietary Cod Protein Restores Insulin-Induced Activation of Phosphatidylinositol 3-Kinase/Akt and GLUT4 Translocation to the T...
Protein recoveries and 5′-nucleotidase activity of membrane fractions were similar between dietary groups (Table 1). We found, ... fraction to the plasma membrane (PM) and the T-tubules (TT) isolated by subcellular fractionation of control and insulin- ... We found that Akt activity is decreased (−40%, P , 0.05) in insulin-stimulated muscle of high-fat-fed rats consuming casein or ... However, we found that both high-fat feeding and cod protein consumption modulated Akt activity without affecting the ...http://diabetes.diabetesjournals.org/content/52/1/29.full
CrkRS | Journal of Cell Science
In subcellular fractionation studies the bulk of CrkRS remains in the insoluble fraction of the nucleus, even after nuclease ... We then assayed whether we were able to detect kinase activity in anti-CrkRS immunoprecipitates. We found that anti-CrkRS ... We found that α -amanitin caused similar changes in the immunofluorescence pattern with both SC35 and anti-CrkRS antibodies ( ... We found that anti-CrkRS antibodies exclusively stained the nucleus. Furthermore, CrkRS appeared to be localized in a discrete ...http://jcs.biologists.org/content/114/14/2591
It has been reported that the increase in SphK1 activity was particularly observed in membrane fractions (40). The subcellular ... However, our group (unpublished data) and others found that this agent failed to contract the rat myometrium (45). These ... Specific SphK activity was expressed as picomoles S1P per minute per milligram of protein, and total activity in each fraction ... Measurement of SphK1 activity.. SphK1 activity contained in rat myometrial supernatant, cytosolic, or membrane fractions was ...http://ajpcell.physiology.org/content/292/1/C240
Abstracts of Papers at the sixty-second annual meeting of the society of general physiologists | JGP
... and we found that endogenous TRPM7 channel activity in β cells requires severe Mg2+ and Mg-nucleotide depletion in order to be ... subcellular fractionation shows enhanced expression of NR1 in the striatal nonpostsynaptic plasma membrane fraction from YAC128 ... and anti-VGCC antibody has been found in a significant fraction of PCA patients, its contribution to PCA is still unclear. ... we found evidence for increased extrasynaptic NMDAR activity in YAC128 MSNs compared with YAC18 MSNs. NMDAR synaptic current ...http://jgp.rupress.org/content/132/1/3a
Interactive Fly, Drosophila
... and subcellular fractionation reveals that most tuberin is found in a membrane/particulate (100,000 x g) fraction. ... In order to test if this activity is potentially important in the prevention of the TSC disease, the GAP activity of wild-type ... If this is true, then the RhebGAP activity must be a limiting factor. If Tuberin possesses RhebGAP activity, overexpression of ... Immunoprecipitates of native tuberin contain an activity that specifically stimulates the intrinsic GTPase activity of Rap1a. ...http://www.sdbonline.org/sites/fly/newgene/gigas2.htm
Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and...
Subcellular fractionation and isolation of the cytoskeleton-containing detergent-insoluble fraction was performed according to ... was mainly found in the detergent-soluble cytosolic fraction and showed severely compromised cortical staining (Fig. 5 a, 15). ... Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge. Cell. 128:915-929. doi:10.1016/j.cell.2007.01 ... The detergent-insoluble pellet (P; cytoskeletal fraction) and the detergent-soluble supernatant (S; cytosolic fraction) were ...http://jem.rupress.org/content/208/9/1777
Compositions and methods relating to novel compounds and targets thereof - Glick, Gary D.
It was found in these past studies that in addition to cytotoxic activity, Bz-423 was also cytostatic under some conditions. ... Neither Bz-423 nor antimycin A generated substantial ROS in the subcellular S15 fraction (cytosol and microsomes), and Bz-423 ... The patches are usually found on the arms, legs, trunk, or scalp but may be found on any part of the skin. The most typical ... The compounds show maximum activity at high substrate concentration and minimal activity (e.g., F1Fo-ATPase inhibiting) at low ...http://www.freepatentsonline.com/y2006/0025388.html
... as confirmed by the activity measurements and the Western blot analysis of the subcellular membrane fractions. ... Here, both transporters were absent from the low sucrose density fractions and were found exclusively in nonraft fractions. ... being found exclusively in nonraft fractions. The heavy MVM and LMVM fractions used in this study correspond to the finger-like ... hENT2 immunoreactivity was found mainly in nonraft fractions, although it could be detected in some, but not all, lipid raft ...http://molpharm.aspetjournals.org/content/80/5/809
AGAP3 and Arf6 Regulate Trafficking of AMPA Receptors and Synaptic Plasticity | Journal of Neuroscience
We found that AGAP3 GTPase domain regulates Ras/ERK signaling previously reported to be essential for activity-dependent ... Next, AGAP3 was detected on subcellular fractions from brain. Using antibodies targeted against either AGAP3 N terminus or C ... 1C). This finding suggests that AGAP3 and SynGAP interact directly and that SynGAP regulates AGAP3 GTPase activity. To ... These findings provide an exciting avenue of study for finding common mechanisms of basal and activity-dependent regulation of ...http://www.jneurosci.org/content/33/31/12586.full
Its subcellular localization is mainly nuclear, with a small fraction found in the cytoplasm and membrane compartments ( ... While the role of VRK1 in neuronal proliferation depends on its kinase activity, its role in neuronal migration is at least ... Furthermore, we found that VRK1 deficiency in human and mouse leads to downregulation of amyloid-β precursor protein (APP), a ... We found that VRK1 deficiency results in extremely reduced levels of APP mRNA, which could result from reduced transcription ...http://www.jneurosci.org/content/35/3/936
Attenuating homologous recombination stimulates an AID-induced antileukemic effect | JEM
2, E-G). This finding was further supported by subcellular fractionation analysis to measure RAD51 nucleocytoplasmic ... B) Fraction change in body mass of mice treated with DNP-KLH and either 0 mg/kg (left) or 50 mg/kg (right) DIDS. Data represent ... Aberrant expression and mutation-inducing activity of AID in human lung cancer. Ann. Surg. Oncol. 18:2084-2092. doi:10.1245/ ... Requirement of non-canonical activity of uracil DNA glycosylase for class switch recombination. J. Biol. Chem. 282:731-742. doi ...http://jem.rupress.org/content/210/5/1021
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