Human Metabolome Database: Showing metabocard for Chenodeoxycholoyl-CoA (HMDB0006292)
... specific amidation activity of both chenodeoxycholoyl-CoA is always found in the most peroxisome-rich subcellular fractions. ( ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in transferase activity, transferring acyl groups other than amino-acyl groups. Specific function:. Not Available. ... Involved in catalytic activity. Specific function:. Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze ...http://www.hmdb.ca/metabolites/HMDB0006292
JCI Insight - Arginine vasopressin infusion is sufficient to model clinical features of preeclampsia in mice
We found no differences in HIF1A concentrations within soluble nuclear or chromatin-bound subcellular fractions between ... Fetal cardiac activity and maternal uterine and umbilical artery flow velocity were visualized using 2-D images and color ... This finding may support relcovaptan and tolvaptan as novel preventative agents against AVP-mediated subtype(s) of PE. However ... No differences were observed in ALT or AST activities between plasmas from saline- or AVP-infused dams on GD 17.5 (Supplemental ...https://insight.jci.org/articles/view/99403
Pharmacology-Lithium's Effects on Cyclic AMP, Membrane Transport, and Cholinergic Mechanisms | SpringerLink
These enzymes have been found to vary in activity in specific regions of the brain (Weiss and Costa, 1968). Adenyl cyclase has ... and to be associated with subcellular fractions that contain membrane fragments and nerve endings (De Robertis et al., 1967). ... Forn, J., and Valdecasas, F. G., 1971, Effects of lithium on brain adenyl cyclasc activity, Biochem. Pharmacol. 20:2773.PubMed ... Adenyl Cyclase Adenyl Cyclase Activity Acetylcholine Release Cholinergic Mechanism Affective Illness These keywords were added ...https://link.springer.com/chapter/10.1007/978-1-4684-2022-7_6
Inositol 1,4,5-trisphosphate turnover enzymes--activities and subcellular distribution in hepatocarcinogenesis. :...
The total phosphatase activity was found to be distributed between cytosol (15%) and membraneous fractions (75%), with most of ... The total phosphatase activity was found to be distributed between cytosol.... The metabolism of inositol 1,4,5-trisphosphate ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ... Subcellular fractions were purified by sequential steps of differential centrifugation and density gradient fractionation ...http://oxfordindex.oup.com/view/10.1093/carcin/18.12.2447
Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Action of the hormone...
... the incorporation of precursor into RNA in all subcellular fractions other than the nuclear one, restores incorporation of ... After treatment with noradrenaline the increase in radioactivity was found solely in AMP after hydrolysis of microsomal RNA to ... decarboxylase activities and causes a 12-fold increase in cytoplasmic Mn2+-dependent polyadenylate polymerase activity. ... rabbit heart with reserpine causes a decrease of incorporation of labelled precursors into RNA species of subcellular fractions ...http://www.biochemj.org/content/168/3/341
Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex and hypothalamus in...
It was found out that Al-AT activities under impairment of feeding regime depend on several factors including morphofunctional ... On the level of mitochondrial fractions, the enzyme is involved into the complex of compensatory reactions via glucose-alanin ... mitochondrial and cytosol fractions of visual, orbital, motor, limbic areas of brain cortex and hypothalamus of three-month old ... Total and specific activities of alaninaminotransferase (Al-AT) were determined in general tissues, ...http://ijpr.sbmu.ac.ir/article_99.html
NIOSHTIC-2 Publications Search - 20037419 - Characterization of [3H]di-isopropyl phosphorofluoridate-binding proteins in hen...
The recovery of the binding activity in the 160 000-Mr band was found to be similar to the recovery of neurotoxic esterase ... Neurotoxic-effects; Toxic-effects; Protein-biochemistry; Subcellular-fractions; Neurotoxicity; Exposure-assessment; Exposure- ... Di-isopropyl phosphorofluoridate was found to phosphorylate all three of these proteins at rates similar to the rate at which ... However, two other binding proteins with suitable binding characteristics were also found in smaller amounts, one of which has ...https://www.cdc.gov/niosh/nioshtic-2/20037419.html
Existence of a reverse transcriptase-p30 complex in AKR mice with a high incidence of spontaneous lymphocytic leukaemia. -...
Significant levels of RT activity were first detected in the microsomal fractions of the two tissues at 15 and 20 weeks old, ... Although low amounts of p30 could be found in both tissues within the first week of life, the overall increase in the amount of ... In addition, a protein complex consisting of p30 and RT was first found in thymus and spleen lymphocytes of 15 and 20 week-old ... The complex could not be found in lymphocytic tissue taken from younger animals or in 'non-target' organs, such as liver or ...https://www.semanticscholar.org/paper/Existence-of-a-reverse-transcriptase-p30-complex-in-Bandyopadhyay-Fish/f638187057b3967c0f3de6c6b931e918b5d1baf3
Publishers of academic thesis & dissertations. Free search & preview. PDF downloads. Instant access to paperback & ebook...
To investigate this in a human genetic context, techniques were established for isolating distinct subcellular fractions of the ... These studies identified a pattern of activity-associated miRNA expression changes unique to the neurites that was revealed to ... Interestingly, some miRNA were found to be preferentially enriched in the nucleus. A motif detected within these sequences lead ... a role for miRNA and the components of PTGS in the functional compartmentalisation of neurons and the response to activity, ...http://www.dissertation.com/abstracts/1032692
Freshly isolated hPT cells exhibited high levels of GSH-dependent enzyme activities, and subcellular fractions prepared from ... In other studies (Lasker et al., 2000), we found renal CYP4A11 to be localized exclusively in the S2 and S3 segments of the ... 5 times higher than hexokinase activity at passage 4. GGT activity remained relatively constant through passage 4. GRD activity ... Activities of GSH-Dependent Enzymes in Freshly Isolated hPT Cells.. Activities of GGT, GCS, GST, GRD, GPX, and hexokinase were ...http://jpet.aspetjournals.org/content/293/2/677
Protocols and Video Articles Authored by Lei Cao (Translated to Japanese)
The wild type form of RAI1 was found in the chromatin bound and nuclear matrix subcellular fractions while the mutant product ... We found this effect in melanoma and colon cancer models, and that it was not caused by physical activity alone. Serum from ... Disease activity was measured using the Crohn's Disease Activity Index (CDAI). Regression analyses of REE with CDAI score, C- ... subcellular localization and transcriptional activity. Clinical features of patients with or without 17p11.2 deletions and ...https://www.jove.com/author/Lei_Cao?language=Japanese
AGPAT enzyme activity could not be demonstrated, and instead the protein was found to have GPAT activity with NEM sensitivity ( ... 2). Evidence for GPAT3 localization to the ER includes immunofluorescence and Western blots of subcellular fractions (17, 113 ... Lipin-1 PAP activity is modulated by factors that influence its subcellular localization. Prior to the identification of the ... GPAT2 activity was first identified as the mitochondrial GPAT activity remaining in Gpat1−/− mice (73). In contrast to GPAT1, ...http://ajpendo.physiology.org/content/296/6/E1195
Sphingomyelinase Activity of Trichomonas vaginalis Extract and Subfractions
All fractions (TE, P30, and S30) have this activity. But it was found that sphingomyelinase activity was higher in TE (2.57 U/ ... Figure 3: Time-curse of SMase activity from the P30 subcellular fraction. Fractions of P30 containing 400 μg of proteins total ... Preparation of Subcellular Fractions. The subcellular fractions were prepared as described previously . Briefly, pellet ... It was found that these fractions of T. vaginalis have SMase activity, which comes principally from P30 subfraction and was ...https://www.hindawi.com/journals/bmri/2013/679365/
... enzyme activity with enzyme content data compared to those extrapolated from the data obtained from subcellular fractions or ... 2001) Non-synonymous single nucleotide alterations found in the CYP2C8 gene result in reduced in vitro paclitaxel metabolism. ... This suggests that, if any developmental changes in CYP2C8 and CYP1A2 activity occur after about 35 days and 15 months ... 2013) Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic ...http://dmd.aspetjournals.org/content/45/5/468
Characterization of an L-Amino Acid Oxidase in Equine Spermatozoa1
This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a ... of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to ... The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live ... These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the ...https://insights.ovid.com/biorep/201505000/00001677-201505000-00015
Subcellular localization of an intracellular serine protease of 68 kDa in Leishmania (Leishmania) amazonensis promastigotes
... but the highest activity was found in the P-35,000 g fraction. This later fraction corresponded to the membranous vesicles and ... Enzymatic activity detection in subcellular fractions - Previous experiments showed that extracts of L. amazonensis were able ... The finding that the enzymatic activity displayed no latency when vesicle fraction was incubated with Triton X-100 (data not ... In order to identify the L. amazonensis protease activity in the subcellular fractions, inhibition experiments were carried out ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000400007
In addition, we found that the increase in PDE3 activity was limited to the subcellular fraction expressing only PDE3B, and not ... Subcellular fractionation revealed that cp/cp VSMC particulate PDE3 activity was increased relative to +/?-derived VSMCs. ... PDE3 and PDE4 activities in JCR:LA-cp rat aortae.. Aortic cAMP PDE activity of the JCR:LA-cp rat strain was predominantly made ... When PDE3 and PDE4 activities were measured in isolated cytosolic and particulate fractions of aortic tissue or of VSMCs, ...http://diabetes.diabetesjournals.org/content/51/4/1194
... this activity is measured in a test tube under optimal conditions using a subcellular fraction as the enzyme source. Without ... We found that any precursor that will directly generate a steady stream of methylselenol is more active than selenite or ... As shown in Table 6 ⇓ , selenium deficiency caused a marked drop in the activities of both enzymes. Sodium selenite was used as ... Ip C., Ganther H. E. Activity of methylated forms of selenium in cancer prevention. Cancer Res., 50: 1206-1211, 1990. ...http://cancerres.aacrjournals.org/content/60/11/2882
Asialoglycoprotein | definition of asialoglycoprotein by Medical dictionary
Topology of asialoglycoprotein receptor in rat liver subcellular fractions: a ferritin immunoelectron microscopic study.. ... silencing activity was found to be restored by the use of an exogenous GalNAc ligand that targets the iLNP to the ... Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein. (ā'sī-al'ō-glī'kō-prō'tēn), A glycoprotein without a sialic acid moiety; ... 03 mg/kg of recombinant ApoE was able to fully restore the activity of an siRNA formulated in an iLNP; and,aA alternatively, in ...http://medical-dictionary.thefreedictionary.com/asialoglycoprotein
892. Carbendazim (Pesticide residues in food: 1995 evaluations Part II Toxicological & Environmental)
... and subcellular fractions were prepared as described above. Wet liver weights and the activities of microsomal cytochrome P450 ... Although haemangiomas of the liver were found in all treated groups but not in controls, no dose-related response was evident. ... subcellular fractions isolated from the liver. The mean absolute liver weights were elevated in males and females fed 1000 or ... The activities of the phase-II drug metabolizing enzymes glucuronyl transferase I and II and the glutathione content were ...http://www.inchem.org/documents/jmpr/jmpmono/v95pr04.htm
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... Proton and calcium transport activities in the fraction were characterized by fluorescence microscopy and spectrophotometric ... as we can now isolate highly purified fractions that could be used for proteomic analysis to find proteins that may clarify the ... We report the purification and characterization of an acidocalcisome fraction from T. gondii tachyzoites after subcellular ...https://www.jove.com/visualize/abstract/21541023/calcium-uptake-proton-transport-acidocalcisomes-toxoplasma
Differential Effects of B Cell Receptor and B Cell Receptor-FcγRIIB1 Engagement on Docking of Csk to GTPase-activating Protein ...
... this complex is found in subcellular membrane or cytoskeletal fractions. Thus, GAP.A-p62 may represent a docking protein for ... 1991) Protein-tyrosine kinases regulate the phosphorylation, protein interactions, subcellular distribution, and activity of ... only a fraction of total cellular Src-related kinase activity is present in such complexes and negatively regulated by Csk. The ... exhibit increased activity of Src and related kinases ((19), (20)). In response to extracellular signals, the activity of Csk ...http://jem.rupress.org/content/186/2/259
General | High-Throughput Screen for the Chemical Inhibitors
... em M sildenafil on cAMP-PDE activity in both subcellular fractions from rat MPA. The next arguments ought to be considered: (1 ... Both cAMP- and cGMP-PDE actions can be found in rat MPA and so are considerably higher in cytosolic than microsomal fractions. ... Sildenafil inhibited the cGMP-PDE activity in both subcellular fractions. This inhibitory impact shows up mainly linked to PDE5 ... 4promoter trans-activation and enzyme activity. (promoter activity. promoter activity was identified in Personal computer12 ...http://acancerjourney.info/index.php/category/general/
The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver |...
... and no endogenous activity was detected in either purified Golgi apparatus or plasma membrane fractions. Limited protease ... we have examined the site and topology of dihydroceramide synthesis in well-characterized subcellular fractions from rat liver ... The distribution of dihydroceramide synthesis was found to exactly parallel that of an endoplasmic reticulum (ER) marker upon ...http://www.biochemj.org/content/290/3/751
CrkRS | Journal of Cell Science
In subcellular fractionation studies the bulk of CrkRS remains in the insoluble fraction of the nucleus, even after nuclease ... We then assayed whether we were able to detect kinase activity in anti-CrkRS immunoprecipitates. We found that anti-CrkRS ... We found that α -amanitin caused similar changes in the immunofluorescence pattern with both SC35 and anti-CrkRS antibodies ( ... We found that anti-CrkRS antibodies exclusively stained the nucleus. Furthermore, CrkRS appeared to be localized in a discrete ...http://jcs.biologists.org/content/114/14/2591