COS cells - Wikipedia
Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying ... COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a ... The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym "COS" is derived from the cells ... and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can ...https://en.wikipedia.org/wiki/COS_cells
GFP-Shank forms filaments in COS cells. A-H, COS cells were transfected with the same constructs as those shown in Figure 1 and ... Shank1B forms filaments in COS cells, but is localized to the synapses of mature neurons. COS cells do not express Shank1 ... GFPSh1BΔSH3 and GFPSh1BΔANK form filaments in COS cells only when coexpressed with full-length Shank1B. A, B, COS cells were ... Only GFP-Shank1B, GKAP1A, and wild-type PSD-95 are distributed in clusters when coexpressed in COS cells. A, B, COS cells were ...http://www.jneurosci.org/content/24/42/9391
Gene Report for G00000032 - Genes2Cognition Neuroscience Research Programme
Co-expression of PSD-95 causes ErbB4 to form clusters in COS cells. We propose that PDZ domain-containing proteins play a role ... Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little ... cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the ... We detected these proteins, apart from SAP102, in parathyroid chief cells, a cell type having a marked expression of megalin. ...http://www.genes2cognition.org/db/Gene/G00000032
PAM polyclonal antibody - (PAB7587) - Products - Abnova
Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells. ...http://www.abnova.com/products/products_detail.asp?catalog_id=PAB7587
Peptidylglycine alpha-amidating monooxygenase - Wikipedia
... cloning and functional expression of a truncated form in COS cells". Biochem Biophys Res Commun. 169 (2): 551-8. doi:10.1016/ ... Cell. Biochem. 55 Suppl (S1994A): 19-28. doi:10.1002/jcb.240550004. PMID 7929615. Ouafik LH, Stoffers DA, Campbell TA, Johnson ...https://en.wikipedia.org/wiki/Peptidylglycine_alpha-amidating_monooxygenase
GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling.
GKAP and PSD-95 formed co-clusters when overexpressed together in COS cells5 (cluster formation in 95% cells examined; see d1 ... Furthermore, S54&S201D mutant failed to form co-clusters with PSD-95 in COS cells (cluster formation in , 1% cells; d5 in Fig. ... COS Cells. Cells, Cultured. Cercopithecus aethiops. Double-Blind Method. Enzyme Activation / physiology. Guanine Nucleotide ... 5a, when tested in COS cells, MVa RNAi specifically reduced MVa levels but had effect on neither PSD-95 nor GKAP. When ...http://www.biomedsearch.com/nih/GKAP-orchestrates-activity-dependent-postsynaptic/23143515.html
Repulsion and Attraction of Axons by Semaphorin3D Are Mediated by Different Neuropilins In Vivo | Journal of Neuroscience
Npn-1 and Npn-2 are capable of forming homodimers or heterodimers when coexpressed in COS cells in the absence of ligand (Chen ... 1B). Each nucMLF extends axons caudally, away from the sema3D-expressing cells, forming the two MLFs that extend on either side ... Drazba J, Lemmon V (1990) The role of cell adhesion molecules in neurite outgrowth on Muller cells. Dev Biol 138: 82-93. ... Shimizu M, Murakami Y, Suto F, Fujisawa H (2000) Determination of cell adhesion sites of neuropilin-1. J Cell Biol 148: 1283- ...http://www.jneurosci.org/content/24/39/8428.long
Interestingly, reticulon proteins can form aggregates when transfected into COS-7 cells, especially Rtn2. Reticulon aggregate- ... For Western blots of COS-7 cell lysates, COS-7 cells were transfected with the empty vector pcDNA3.1mychis or constructs ... Cell culture.. COS-7 cells (ATCC) were cultured in DMEM with 10% FBS, 1 mm glutamine, and penicillin/streptomycin at 37°C in a ... n = 3 experiments in all cases, 124-224 cells per antigen. D, Immunoblot of COS-7 cell lysates probed with antibody against ...http://www.jneurosci.org/content/29/44/13850
1C). In protein coclustering assays, COS cells doubly expressing PSD-95 and TANC2 formed discrete intracellular clusters where ... D, TANC2 coclusters with PSD-95 in heterologous cells in a manner requiring the PDZ-dependent interaction. COS cells were ... TANC1 and TANC2 form a complex with PSD-95 in heterologous cells. HEK293T cell lysates doubly transfected with FLAG-tagged ... Dopamine-Dependent Sensitization of Rod Bipolar Cells by GABA Is Conveyed through Wide-Field Amacrine Cells ...http://www.jneurosci.org/content/30/45/15102
Monoclonal antibodies that block ligand binding to the CD22 receptor in mature B cells - Dana-Farber Cancer Institute, Inc.
... average number of cells bound per COS cell or by determining the mean number of test cells bound per rosette-forming COS cell. ... Adhesion of blood cells and cell lines to COS-CD22 cells Cell attachment to:a Cell expression of:b COS COS-CD22 CD45RO CDw75 ... As shown in Table I, blood T cells and spleen B cells bound avidly to the COS-CD22 cells while they did not attach to COS cells ... 1 shows binding of various cell types and cell lines to untransfected COS cells, to COS cells transfected with CD22 cDNA, and ...http://www.freepatentsonline.com/5484892.html
Marinesco-Sjögren Syndrome - GeneReviews® - NCBI Bookshelf
In transiently transfected COS-1 cells the mutated formed aggregates within the ER implying that aggregation of the mutated ... DNA banking is the storage of DNA (typically extracted from white blood cells) for possible future use. Because it is likely ... A truncation of Sil1 was shown to cause ataxia and cerebellar Purkinje cell loss in naturally-occurring woozy mutated mouse [ ...https://www.ncbi.nlm.nih.gov/books/NBK1192/
Mitochondrial Networking Protects β-Cells From Nutrient-Induced Apoptosis | Diabetes
Previous studies have reported that β-cell mitochondria form less elaborate network structures, compared with COS cells for ... Under normal condition, the dynamic activity of mitochondria is evident in clonal β-cells, dispersed β-cells, and β-cells ... of cells after 4 h and in 51, 61, and 79% of cells after 24 h, respectively (Fig. 4A). Only 16 and 28% of cells exposed to 20 ... a β-cell within an islet, and an INS1 cell. Under noxious conditions, we find that β-cell mitochondria become fragmented and ...http://diabetes.diabetesjournals.org/content/58/10/2303.long
SMART: CASc domain annotation
Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature form. Sequence analysis ... cell surface receptors on cells targeted to die by various TNF cytokines that are produced by cells such as cytotoxic T cells. ... Programmed cell death (apoptosis) is a controlled process by which unwanted cells are selectively eliminated. Several families ... elegans ced-3 gene causes Rat-1 cells to undergo programmed cell death. Point mutations in a region homologous between mICE and ...http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=CASc&BLAST=DUMMY
The correctly folded portions of the opsin isolated from COS cells bound 11-cis-retinal to form rhodopsin-like chromophore and ... The RP mutations cause partial to total misfolding of the corresponding opsins when their genes were expressed in COS cells. ... It was shown previously that these two pathways in COS-1 cells are coupled to two different G-proteins (Perez et al., 1993). It ... GTPγS binding assay using cell membranes from CHO cells stably expressing the wild-type or a W284L mutant human δ-opioid ...http://pharmrev.aspetjournals.org/content/57/2/147
WikiGenes - TUBB3 - tubulin, beta 3 class III
... alpha 6 beta 4 forms a complex with the cytoskeletal protein HD1 and induces its redistribution in transfected COS-7 cells. ... On the other hand, the expression of myr-AKT in MCF7 beta4-short hairpin RNA cells rescues the cells from apoptosis in the ... Cell (1997) [Pubmed]. *Loss of co-localization of alpha 6 beta 4 integrin and collagen VII in bladder cancer. Liebert, M., ... Cell. Endocrinol. (2007) [Pubmed]. *Estrogen receptor (ER)-beta isoforms: a key to understanding ER-beta signaling. Leung, Y.K ...https://www.wikigenes.org/e/gene/e/10381.html
Expression of the Vesicular Inhibitory Amino Acid Transporter in Pancreatic Islet Cells | Diabetes
Transfected COS-7 cells were also found to synthesize the lower-molecular weight molecule (20). It is unclear how the two ... Our results indicate that there are at least two forms of the VIAAT protein; these migrate at ∼57 and ∼52 kDa. Both forms were ... although at lower levels than β-cells (33,39). Here, using islets depleted of β-cells, we confirm that rat islet non-β-cells ... where glucagon-producing α-cells and other non-β-cells are localized. VIAAT was also present in the β-cells but in a lower ...http://diabetes.diabetesjournals.org/content/51/6/1763
KAKEN - Research Projects | Regulation Mechanism of Cyclin Dependent Kinase Activity in Mammalian Cells (KAKENHI-PROJECT...
When both weel kinase and 14-3-3 zeta were transfected into COS-1 cells, they formed a complex in a cell. The weel kinase ... is phosphorylated in mammalian cells by protein kinase Cdc2.' J.Cell Sci.108. 2145-2152 (1995). *. Description. 「研究成果報告書概要(欧文)」 ... cdc2 kinase / cdk2 / cyclin / weel kinase / 14-3-3 / cell cycle / myt1キナーゼ / 核移行 / リン酸化 / サイクリン依存性キナーゼ / 転写因子 / PEA3 / weelキナーゼ ... Cell Res.214.https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454597/
Dissecting the basis of nongenomic activation of endothelial nitric oxide synthase by estradiol: role of ERalpha domains with...
... the potential roles of ERalpha domains necessary for classical nuclear function were investigated in COS-7 cells. In cross- ... linking studies, estradiol-17beta (E2) caused PM-associated ERalpha to form dimers. However, eNOS activation by E2 was ...https://www.ncbi.nlm.nih.gov/pubmed/15486047
Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7 | JCB
... we performed GST pull-downs of extracts from COS-7 cells expressing different combinations of epitope-tagged forms of Vps26, ... and pEF-HA-Vps35 in COS-7 cells using Lipofectamine-2000 (Invitrogen). After 24 h, the cells were washed with ice-cold PBS and ... caused substantial increases in the amount of precursor and intermediate forms present within the cells and of precursor form ... control cells or cells expressing GFP-Rab7-Q67L were incubated with 0.1% DMSO or 200 nM wortmannin in DMSO for 30 min. Cells ...http://jcb.rupress.org/content/183/3/513
Frontiers | Interaction of Protease-Activated Receptor 2 with G Proteins and β-Arrestin 1 Studied by Bioluminescence Resonance...
These data demonstrate the coupling of PAR2 with Galphai1, Galphao and Galpha12 in COS-7 cells with differences in the kinetics ... These data demonstrate the coupling of PAR2 with Galphai1, Galphao and Galpha12 in COS-7 cells with differences in the kinetics ... Using YFP and Rluc fusion constructs expressed in COS-7 cells, BRET data revealed a pre-assembly of PAR2 with both Galphai1 and ... Using YFP and Rluc fusion constructs expressed in COS-7 cells, BRET data revealed a pre-assembly of PAR2 with both Galphai1 and ...https://www.frontiersin.org/articles/10.3389/fendo.2013.00196/full
Paracellular route is the major urate transport pathway across the blood-placental barrier.
TER of COS‐7 cell monolayers, which do not form tight junctions (negative control), was 218Ω. In contrast, BeWo cell monolayers ... 7 cell monolayers but not BeWo or MDCK cell monolayers (Fig. 4). These results indicate that BeWo cells form functional tight ... COS‐7 cells and MDCK cells (ATCC) were cultured in a humidified incubator under 5% CO2 in Dulbecco's Modified Eagle's Medium ( ... Cell lines were cultured on Transwell cell culture inserts to full confluency. Cells were chilled and washed with uptake ...http://www.biomedsearch.com/nih/Paracellular-route-major-urate-transport/24844637.html
Protocols and Video Articles Authored by Imre Berger (Translated to Dutch)
In hypoosmotically stressed COS cells with increased levels of PI(3,5)P2, Mtmr2 is bound to the membrane of vacuoles formed ... multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell ... resulting in homogenous cell populations. Analysis of multiple parameters in living cells may require co-expression of ... the use of mammalian cells is limited. The baculoviral insect cell system has proven to be a powerful alternative for the ...https://www.jove.com/author/Imre_Berger?language=Dutch
Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...
Polyplexes form a heterogeneous population from which smaller particles can be taken up via the clathrin-mediated pathway and ... COS-7 cells were used in this study as they are a well-established model cell line for gene transfer research. Both pDMAEMA and ... COS-7 cells were incubated with pDMAEMA- or PEI-based polyplexes for 60 minutes at 4°C. Subsequently, the cells were incubated ... Cell Culture. COS-7 African green monkey cells were grown in DMEM (Gibco BRL, Breda, The Netherlands) supplemented with ...https://link.springer.com/article/10.1007%2Fs11095-007-9287-3
Gene Report for G00001491 - Genes2Cognition Neuroscience Research Programme
Two forms of protein kinase C-delta were isolated from COS cells and characterized by differences in gel mobility, GAP-43 ... GAP-43 expressed in either PC12 or COS-1 cells was acetylated at the N-terminal methionine. Approximately 35% of the N-terminal ... the integration of data from cell-based screening assays addressing the influence of protein-overexpression on cell ... Only some Merkel cells and Meissner corpuscles were GAP-43-immunoreactive. In conclusion, our results show that GAP-43 protein ...http://www.genes2cognition.org/db/Gene/G00001491
APLP1 - Wikipedia
... ectopic expression in COS-7 cells and identification of soluble forms in the cerebrospinal fluid". Eur. J. Biochem. 250 (2): ... of the amyloid precursor-like protein APLP2 and reduction of APLP1 in retinoic acid-differentiated human neuroblastoma cells ...https://en.wikipedia.org/wiki/APLP1