Conducting atomic force microscopy study of silicon dioxide breakdown - CUED Publications database
Atta, RM and O'Shea, SJ and Murrell, MP and Welland, ME (1995) Conducting atomic force microscopy study of silicon dioxide breakdown. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, 13. pp. 1945-1952. ISSN 1071-1023. Full text not available from this repository ...http://publications.eng.cam.ac.uk/337498/
Plastic Deformation Quantified by Atomic Force Microscopy Measurements for Duplex Stainless Steel under Monotonic and Cyclic...
Advances in Experimental Mechanics VI: Plastic Deformation Quantified by Atomic Force Microscopy Measurements for Duplex Stainless Steel under Monotonic and Cyclic Loadinghttps://www.scientific.net/AMM.13-14.163
Design and characterization of a novel scanner for high-speed atomic force microscopy - ePrints Soton
Schitter, Georg, Fantner, Georg E., Thurner, Philipp J., Adams, Johnathan and Hansma, Paul K. (2006) Design and characterization of a novel scanner for high-speed atomic force microscopy At Mechatronics 2006: 4th IFAC-Symposium on Mechantronic Systems. 12 - 14 Sep 2006. ...https://eprints.soton.ac.uk/49048/
Park Systems 2014 Announces Atomic Force Microscopy Image Contest Winner
Park Systems, a leader in Atomic Force Microscopy (AFM) since 1997 announces their first AFM image contest winner, Namuna Panday, a Graduate Student at Florida International University. Her winning submission is an AFM image taken using Park XE Bio of a HeLa Cell used in her research studying drug delivery methods for cancer research.https://www.nanowerk.com/nanotechnology-news/newsid=34635.php
Nuit Blanche: Magni: A Python Package for Compressive Sampling and Reconstruction of Atomic Force Microscopy Images -...
Magni is an open source Python package that embraces compressed sensing and Atomic Force Microscopy (AFM) imaging techniques. It provides AFM-specific functionality for undersampling and reconstructing images from AFM equipment and thereby accelerating the acquisition of AFM images. Magni also provides researchers in compressed sensing with a selection of algorithms for reconstructing undersampled general images, and offers a consistent and rigorous way to efficiently evaluate the researchers own developed reconstruction algorithms in terms of phase transitions. The package also serves as a convenient platform for researchers in compressed sensing aiming at obtaining a high degree of reproducibility of their research ...http://nuit-blanche.blogspot.com/2014/10/magni-python-package-for-compressive.html
Atomic Force Microscopy Investigation of NI Particles Deposited on Porous Silicon
In this thesis properties and influence of modification techniques of porous silicon were studied by Atomic Force Microscope (AFM). This device permits to visualize the surface topography and to study properties of the samples on atomic scale, which was necessary for recent investigation. Samples of porous silicon were obtained by electrochemical etching. Nickel particles were deposited by two methods: electrochemical deposition and extracting from NiCl2 ethanol solution. Sample growth was conducted in Saint-Petersburg State Electrotechnical University, LETI ...http://www.doria.fi/handle/10024/105172
Understanding the Mechanical Properties of Microalgae Using Atomic Force Microscopy | Volume 1A: Abdominal Aortic Aneurysms;...
From consumer productions to energy production, algae is used in many industrial processes. Understanding the mechanical behavior of algae is important to optimize these processes. To obtain a better understanding of algae cell response, we mechanically characterized single, dried Scenedesmus dimorphus cells. To accomplish this, we used atomic force microscopy (AFM) to image S. dimorphus cells, which enabled us to map the AFM measurements to a location on the individual cells. We were then able to perform force measurements on the AFM to determine the Young's modulus of S. dimorphus. These findings enable a more detailed understanding of the mechanical properties of a single S. dimorphus cell, which may be useful in many applications.. Copyright © 2013 by ASME ...http://proceedings.asmedigitalcollection.asme.org/proceeding.aspx?articleid=1819979
A Study on the Mechanism of Small Fatigue Crack Deflection Behavior in Alpha-Brass by Means of In Situ Atomic Force Microscopy...
Progresses in Fracture and Strength of Materials and Structures: A Study on the Mechanism of Small Fatigue Crack Deflection Behavior in Alpha-Brass by Means of In Situ Atomic Force Microscopy and Crystallo-Graphic Orientation Analysishttps://www.scientific.net/KEM.353-358.1225
Atomic Force Microscopy studies of proteins immobilized on silica by thiol-disulfide exchange
In this thesis methods for investigation of orientation and conformation of individual macromolecules on surfaces are presented as well as novel methods for functionalization of silicon chips with the possibility to get an ordered immobilization of antibodies. Two novel methods are presented which makes it possible to investigate the orientation of individual macromolecules on different kinds of surfaces with AFM. One is based on threshold patterning where, depending on substrate, side- and end-on adsorbed immunoglobulin molecules could be detected. The other method is using the principle of site-specific ligands where the orientation of proteins adsorbed to various surfaces was evaluated. By measuring the increase in protein volume of the formed protein-ligand complexes with AFM, the amount of protein having an orientation that allows binding can be estimated. The influence of surface chemistry on protein structure was examined using human serum fibronectin adsorbed to hydrophilic and ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:161417
Публикации за 2009 год. Strekal', N.; Kulakovich, O.; Askirka, V.; Sveklo, I.; Maskevich, S. Features of the Secondary Emission Enhancement Near Plasmonic Gold Film, - Plasmonics, 2009, Vol. 4, pp. 1-7.. A.V. Belko, A.V. Nikitin, N.D. Strekal, A.E. German. Fractal Structure of Gold Clusters Formed under Vacuum Deposition on Dielectric Substrates // X-ray, Synchrotron and Neutron Techniques, 2009, Vol. 3, No. 3, pp. 338-342.. A Hyper-Raman Spectroscopy, Spectrophotometry and Atomic Force Microscopy Study of Photochromism of Spirocyclic Compounds on Nanostructured Metal Films / G.T. Vasilyuk, S.A. Maskevich, A.E. German, I.F. Sveklo, B.S. Lukyanov, L.A. Ageev. // High Energy Chemistry, 2009,Vol.43, No.7, pp.623-627.. ...http://www.lms.grsu.by/index.php/osnovnye-publikatsii
"The Role of Histone H4 Biotinylation in the Structure of Nucleosomes" by Nina A. Filenko, Carol Kolar et al.
Background: Post-translational modifications of histones play important roles in regulating nucleosome structure and gene transcription. It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes. We hypothesized that biotinylation modifies the physical structure of nucleosomes, and that biotin-induced conformational changes contribute to gene silencing associated with histone biotinylation. Methodology/Principal Findings: To test this hypothesis we used atomic force microscopy to directly analyze structures of nucleosomes formed with biotin-modified and non-modified H4. The analysis of the AFM images revealed a 13% increase in the length of DNA wrapped around the histone core in nucleosomes with biotinylated H4. This statistically significant (p,0.001) difference between native and biotinylated nucleosomes corresponds to adding approximately 20 bp to the classical 147 bp length of nucleosomal ...http://digitalcommons.unl.edu/nutritionfacpub/32/
Stepwise Unfolding of Ankyrin Repeats in a Single Protein Revealed by Atomic Force Microscopy | Fernandez Lab
Using single-molecule atomic force microscopy, we find that a protein consisting of six identical ankyrin repeat units flanked by N- and C-terminal modules (N6C) unfolds in a stepwise, unit-by-unit fashion under a mechanical force. Stretching a N6C molecule results in a sawtooth pattern fingerprint, with as many as six peaks separated by ~10 nm and an average unfolding force of 50 ± 20 pN. Our results demonstrate that a stretching force can unfold multiple repeat units individually in a single protein molecule, despite extensive hydrophobic interactions between adjacent units ...http://fernandezlab.biology.columbia.edu/publication/stepwise-unfolding-ankyrin-repeats-single-protein-revealed-atomic-force-microscopy
Enhanced efficiency in the excitation of higher modes for atomic force microscopy and mechanical sensors operated in liquids ...
Recent developments in dynamic Atomic Force Microscopy where several eigenmodes are simultaneously excited in liquid media are proving to be an excellent tool in biological studies. Despite its relevance, the search for a reliable, efficient, and strong cantilever excitation method is still in progress. Herein, we present a theoretical modeling and experimental results of different actuation methods compatible with the operation of Atomic Force Microscopy in liquid environments: ideal acoustic, homogeneously distributed force, distributed applied torque (MAC Mode™), photothermal and magnetostrictive excitation. From the analysis of the results, it can be concluded that magnetostriction is the strongest and most efficient technique for higher eigenmode excitation when using soft cantilevers in liquid media. ...https://www.osti.gov/scitech/biblio/22310669-enhanced-efficiency-excitation-higher-modes-atomic-force-microscopy-mechanical-sensors-operated-liquids
Plasmid AFM [image] | EurekAlert! Science News
An atomic force microscopy image shows a four-part complex of the protein RepA (bright mounds) bound together and 'handcuffing' two thread-like strands of DNA plasmid.https://www.eurekalert.org/multimedia/pub/74483.php?from=269627
JAIRO | Evaluation of peptide-material interaction by force mapping method with an atomic force microscope
Evaluation of peptide-material interaction by force mapping method with an atomic force microscopeEvaluation of peptide-material interaction by force mapping method with an atomic force microscope ...http://jairo.nii.ac.jp/0083/00235857/en
DIGITAL.CSIC: Mechanical properties of viruses analyzed by atomic force microscopy: A virological perspective
The advent of nanoscience and nanotechnology and the development of atomic force microscopy and other single-molecule techniques are leading to a renewed look at viruses from the point of view of the physical sciences. As any other solid-state object, virus particles are endowed with mechanical properties such as elasticity or brittleness. Emerging studies on virus mechanics may facilitate the engineering of the physical properties of viruses to improve their potential application in nanotechnology, and may be also relevant to understand virus biology. Viruses are subject to internal and external forces, and as evolving entities they may have selectively adapted their mechanical behavior to resist, or even use, those forces. This article adopts the perspective of structural and molecular virology to review the results obtained to date, using the atomic force microscope, on the mechanical properties of virus ...https://digital.csic.es/handle/10261/113860
NSF CMMI: Mechanical Phenotyping of Cells: Haptics-Enabled Atomic Force Microscopy | Institute for Systems Research
The research objective of this award is to investigate novel approaches for using Atomic Force Microscopy (AFM), combined with haptic (sense of touch) feedback, to mechanically phenotype and monitor individual cells. To enable this, one of the primary tasks in this project will be to develop a magnetically levitated haptic feedback device with near zero friction and sufficient fidelity so that a user can ?feel? individual cells. Furthermore, in the AFM studies, we will monitor the stiffness of the different cell types for both local and global responses. For global stiffness measurements, we will use modified AFM cantilevers whereby the diameter of the ball at the tip will be significantly larger than that of the cell. We will use localized measurements to understand the variation in the stiffness at various locations on the cell surface for each type of cell. We will use the Hertz contact model to quantify the mechanical response of the cell for local and global cell probing tasks. Such ...http://www.isr.umd.edu/node/854
Nanoscale characterization of solution-cast poly(vinylidene fluoride) thinfilms using atomic force microscopy
This thesis research focuses on the characterization of thinfilms made of poly(vinylidene fluoride) (PVDF) using an atomic force microscope. Thinfilms of PVDF were fabricated by a spin coating method with different conditions and characterized using the Atomic Force Microscopy (AFM) for morphological changes. Phase and conformational changes of PVDF were investigated using both wide angle X-ray diffraction (WAXD) and Fourier Transform Infrared Spectroscopy (FTIR). From this analysis, in-situ corona poling with annealing of spin-cast PVDF enabled a phase change from Ã Â± to the mixture of Ã Â² and Ã Â³ phases. This process can decrease the complexity of the conventional method which requires mechanical stretching before poling PVDF in addition to thermal annealing for Ã Â² phase transformation. This thesis describes some materials and surface properties of solution-cast PVDF thinfilms with various conditions such as topography and phase image, adhesion force, ...http://oaktrust.library.tamu.edu/handle/1969.1/4790
Atomic Force Microscopy Webinars | Bruker
Currently, there is a growing need for methods that can quantify and map the molecular interactions of biological samples, both with high-force sensitivity and high spatial resolution. Force-distance (FD) curve-based atomic force microscopy is a valuable tool to simultaneously contour the surface and map the biophysical properties of biological samples at the nanoscale. In this webinar, we will report the use of advanced FD-based technology combined with chemically functionalized tips to probe the localization and interactions of chemical and biological sites on single native proteins and on living cells at high-resolution. I will present how an atomic force and confocal microscopy set-up allows the surface receptor landscape of cells to be imaged and the virus binding events within the first millisecond of contact with the cell to be mapped at high resolution (,50 nm). I will also highlight theoretical approaches to contour ...https://www.bruker.com/fr/service/education-training/webinars/afm.html
Deflection images of living outer Annulus cells from Bovine Intervertebral discs, day 6 (after seeding), imaged in culture medium containing 10% fetal bovine serum (FBS) using Contact Mode. Left image was acquired before a series of force curves were taken on the area of the cell that is circled in red. Right image was acquired 45 minutes later. Displayed force curve was acquired on the cell marked with an "X". It can be clearly seen that the cells have moved, undergone cytoskeletal reorganization and, where the force curves were taken, were damaged by the AFM tip, 54µm.. Data Courtesy of Y. Dror and J. Klein, Oxford University and Weizmann Institute, NanoInteract consortium.. ...http://www.asylumresearch.com/Gallery/Nanomechanics/ForceCurves/Force12.shtml
Nanotechnology Learning Modules and Atomic Force Microscopy of Neanderthal Stone Tools, NC DOCKS (North Carolina Digital Online...
Abstract: This thesis uses a Veeco Icon Atomic Force Microscope (AFM) to educate undergraduate students about the nanoscale world and to perform archaeological research. In chapter 2, an educational resource is developed to provide hands-on nanotechnology experience for undergraduate students. With the rapid growth of atomic force microscopy at many levels of industry and academia, it is important to expose the next generation to this technique. This learning module attempts to provide an experimental approach to learning about AFM phase imaging and its many applications. In chapters 3 and 4, AFM is used as one of several techniques for classifying the use of Neanderthal flint tools from Weasel Cave, Russia. These stone tools were identified as being used for tasks such as wood working, hide scraping, and meat cutting. Depending on the type of flint and the task involved, various degrees of abrasion occurred, leaving behind microwear polishes. These microwear traces are ...http://libres.uncg.edu/ir/listing.aspx?id=8001
Controlling a Single DNA Molecule in an Electric Field by Means of In Situ Atomic Force Microscopy
DNA is the bio-polymer containing the genetic information needed for the development and functioning for all living organisms. It has a polymeric structure consisting of units called nucleotides, each consisting of a non-polar, hydrophobic interior (the base pairs) and polar, hydrophilic exterior which is negatively charged due to the phosphate groups along the backbone of the DNA. Its heterogeneous properties permit DNA molecule to interact with other molecules and different types of substrates at the same time. It is important to understand the DNA morphology on flat surface that serves as a template for DNA based sensors and microarray applications, particularly under an electric field. Taken together, the ability to deposit a single DNA molecule on the electrode is essential to increase its specificity. In our study, we optimize the conditions in order to control a single DNA molecule adsorbing and desorbing to/from the electrode. Simultaneously dynamic imaging enables us to analyze its ...https://infoscience.epfl.ch/record/184694
Making Atomic Force Microscopy Work For Cells - Redorbit
Atomic force microscopy, a tactile-based probe technique, provides a three-dimensional nanoscale image of a material by gliding a needle-like arm across the material's surface. The core of AFM imaging workhorse is a cantilever with a sharp tip that deflects as it encounters undulations across a surface. Due to a minimum force required for imaging, conventional AFM cantilevers can deform or even tear apart living cells and other biological materials. While scientists have made strides in reducing this minimum force by making smaller cantilevers, the force is still too great to image cells with high resolution. Indeed, for imaging objects smaller than the diffraction limit of light""that is, nanometer dimensions""this approach hits a roadblock as the instrument can no longer sense minute forces.. Now, however, scientists with the Molecular Foundry, a U.S. Department of Energy User Facility located at Berkeley ...http://www.redorbit.com/news/technology/1853246/making_atomic_force_microscopy_work_for_cells/
Imaging and Measuring the Molecular Force of Lymphoma Pathological Cells Using Atomic Force Microscopy.
Atomic force microscopy (AFM) provides a new technology to visualize the cellular topography and quantify the molecular interactions at nanometer spatial resolution. In this work, AFM was used to image the cellular topography and measure the moleculahttp://www.biomedsearch.com/nih/Imaging-Measuring-Molecular-Force-Lymphoma/22890585.html
Thermally induced phase transition in crystalline lead phthalocyanine films investigated by XRD and atomic force microscopy |...
Lead phthalocyanine films have been deposited at room temperature on to silicon substrates and than annealed in high vacuum at different temperatures. The samples have been analysed by X-ray diffraction (XRD) as well as atomic force microscopy (AFM) in order to get structural and morphological information on the samples. The as deposited film shows a monoclinic structure with a (320) orientation. Increasing the annealing temperature up to 170 degrees C, crystallites with both triclinic and monoclinic phases are observed, although the triclinic phase seems unstable. Annealing the sample at 190 degrees C the film consists mainly of monoclinic phase. The AFM topographic images suggest that at this temperature the triclinic crystallites evaporate, leaving wide holes in the films. (C) 1998 Elsevier Science B.V. All rights reserved.. ...https://ricerca.univaq.it/handle/11697/15647