Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides | BMC Biochemistry | Full Text
10 μg of crude protein extracts of HeLa cells transfected with PDE12ΔmTP-His, red-fluorescent protein (DsRed), empty-vector ... Zilberstein A, Kimchi A, Schmidt A, Revel M. Isolation of two interferon-induced translational inhibitors: a protein kinase and ... Synthesis of 2-5As was performed with a human OAS1 isoform known to produce oligomers up to 30-mers in vitro . The OAS2 and ... Similarly, the A(pA)3 will be committed to the acceptor-site due to a lack of 5′ phosphates. Consequently, the single A(pA)3pdA ...https://bmcbiochem.biomedcentral.com/articles/10.1186/s12858-015-0043-8
The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance | SpringerLink
Within the past decade, ectonucleotidases belonging to several enzyme families have been... ... The future planned use of transgenic mice expressing fluorescent protein under the promoter of the respective NTPDase isoform ... adenylate kinase, nucleoside diphosphate kinase, and potentially ecto-F1-Fo ATP synthases [21, 22, 23, 24, 25] that may ... Enzyme activity is reduced following avoidance learning  and status epilepticus [247, 248]. It is altered in two rat ...https://link.springer.com/article/10.1007%2Fs11302-006-9003-5
Mechanism of Cardiac Sarcolemmal Adenosine Triphosphate-sensitive Potassium Channel Activation by Isoflurane in a Heterologous...
Consequently, it has been demonstrated that under such conditions (pHi 7.4), activation of protein kinase C isoform PKC-ϵ is ... The phosphotransfer enzymes such as creatine kinase or adenylate kinase provide a mechanism to communicate changes in the ... Under our experimental conditions, HEK293 cells transfected with cDNA for green fluorescent protein alone exhibited green ... Zingman LV, Alekseev AE, Bienengraeber M, Hodgson D, Karger AB, Dzeja PP, Terzic A: Signaling in channel/enzyme multimers: ...http://anesthesiology.pubs.asahq.org/article.aspx?articleid=1931115
Optogenetic Reporters: Fluorescent Protein-Based Genetically-Encoded Indicators of Signaling and Metabolism in the Brain
G protein coupled receptors, Rho GTPases), enzyme activities (e.g., protein kinase A, caspases), and reactive species. We focus ... Phosphoinositides can be hydrolyzed to inositol phosphates and diacylglycerol (DAG) in a receptor-mediated manner, and these ... The chloride transporter Na(+)-K(+)-Cl- cotransporter isoform-1 contributes to intracellular chloride increases after in vitro ... Two major downstream pathways involve activation of phospholipase C (PLC) or activation/inhibition of adenylate cyclase. ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3494096/?lang=en-ca
Adenoprotection of the heart involves phospholipase C-induced activation and translocation of PKC-ε to RACK2 in adult rat and...
Steinberg SF, Goldberg M, Rybin VO. The protein kinase C isoform diversity in the heart. J Mol Cell Cardiol 27: 141-153, 1995. ... 1, C and F). A linear fluorescent density scan was conducted over 18.8 μm perpendicular to the t-tubular structures, as ... Romano FD, MacDonald SG, Dobson JG Jr. Adenosine receptor coupling to adenylate cyclase of rat ventricular myocyte membranes. ... and inositol phosphates, suggesting that the A1R is coupled to phospholipase C (PLC) (34). One objective of the present study ...http://ajpheart.physiology.org/content/297/2/H718.long
Application # 2018/0071285. SYSTEMS PHARMACOLOGY FOR TREATING OCULAR DISORDERS - Patents.com
protein kinase C.alpha.), and GFAP (glial fibrillary acidic protein) (all of which are green) was then revealed by ... Dark-adapted Abca4.sup.-/-Rdh8.sup.-/- mice at the age of 4-5 weeks were intraperitoneally injected with the fluorescent ROS ... TABLE-US-00003 TABLE 3 Expression of adrenergic receptors, serotonin receptors, and adenylate cyclases in the eye and retina of ... 0083] Examples of prodrugs include, but are not limited to, esters (e.g., acetate, dialkylaminoacetates, formates, phosphates, ...http://patents.com/us-20180071285.html
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), a Neuron-Derived Peptide Regulating Glial Glutamate Transport and...
... but not on protein kinase A, nerve growth factor receptor tyrosine kinase, p21ras G-protein, and pp60c-src cytoplasmic tyrosine ... In the next set of experiments we asked whether PACAP also affects the expression of the glutamate-converting enzyme GS. To ... receptor isoform, respond to PACAP by proliferation, whereas cerebral neuroblasts, expressing PAC1-R s, cease to divide in the ... and optical densities of the bands were determined by fluorescent image scanning. The ratio of optical densities was calculated ...http://www.jneurosci.org/content/20/10/3596
James Ferrell | Stanford Medicine Profiles
... a 42-kDa protein related to mitogen-activated protein kinase or microtubule-associated protein-2 kinase (MAP kinase) and a 34- ... Progesterone brings about inhibition of adenylate cyclase and activation of the Mos/MEK1/p42 MAP kinase cascade, which ... Selective regulation of neurite extension and synapse formation by the beta but not the of isoform of CaMKII NEURON Fink, C. C ... Probing the precision of the mitotic clock with a live-cell fluorescent biosensor NATURE BIOTECHNOLOGY Jones, J. T., MYERS, J. ...https://med.stanford.edu/profiles/james-ferrell
Gene Report for G00001619 - Genes2Cognition Neuroscience Research Programme
The brain-type cytosolic isoform of creatine kinase (BB-CK), which is found mainly in the brain and retina, is a key enzyme in ... Protein Expression. 1254 (human protein atlas). Protein Sequence. P12277 (UniProt). Literature (41). Pubmed - other. * Defining ... as well as adenylate kinase (myokinase, E.C. 22.214.171.124) were identified in washed spermatozoa from chicken and man by cellulose ... because high-energy phosphates are transferred through the creatine kinase/phosphocreatine shuttle system. The recombinant ...http://www.genes2cognition.org/db/Gene/G00001619
SMART: Secondary literature for RAS domain
GTP activated Ras interacts directly with the Raf protein to recruit the MAP kinases and their subordinates. Attachment of Ras ... and adenylate kinase (ADK). We find that there is a close correspondence between the conformation and position of our predicted ... fluorescent tags) by which the protein can be traced in biological systems, could provide invaluable reagents. We report here ... Four loops are involved in interactions with bound guanosine diphosphate: one with the phosphates, another with the ribose, and ...http://smart.embl.de/smart/show_secondary.cgi?domain=RAS
Interplay between a cytosolic and a cell surface carbonic anhydrase in pH homeostasis and acid tolerance of Leishmania |...
... procedure was checked by immunoblotting with antibodies against cytosolic adenosine kinase and plasma membrane protein Na+/K+- ... P≤0.05, **P,0.01 (Student's t-test). (F) Bar graph showing specific activity of carbonic anhydrase [enzyme unit (EU)/mg] in ... 1990). Effects of carbonic anhydrase inhibitors on contraction, intracellular pH and energy-rich phosphates of rat skeletal ... 2013). Globin-coupled heme containing oxygen sensor soluble adenylate cyclase in Leishmania prevents cell death during hypoxia ...http://jcs.biologists.org/content/130/4/754