The Prolyl Isomerase Pin1 Enhances HER-2 Expression and Cellular Transformation via Its Interaction with Mitogen-Activated...
... a potent Pin1 inhibitor, markedly suppressed the expression of activator protein-2α and the HER-2 promoter activity induced by ... O-tetradecanoylphorbol-13-acetate in MCF-7 cells. Importantly, these inhibitors inhibited the neoplastic cell transformation ... The HER-2 oncogene, a member of the erythroblastosis oncogene B (ERBB)-like oncogene family, has been shown to be amplified in ... Here, we found that Pin1 interacts with mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) ...http://mct.aacrjournals.org/content/early/2010/02/21/1535-7163.MCT-09-0560
O-tetradecanoyl-phorbol-13-acetate; MAPK, p44/42 mitogen-activated protein kinase; Mek, MAP/ERK kinase; PKC, protein kinase C; ... 1) ⇓ . The most potent growth inhibitor of MCF7 cells was ICI and atRA for T47D cells. Steroid hormones did not stimulate the ... v contains many IFN-inducible genes, and vi is a tight cluster of histone family members. Cluster vii represents genes strongly ... mitogen-activated protein/extracellular signal-regulated kinase 1/2 inhibitor U0126 and phorbol ester 12-O-tetradecanoylphorbol ...http://cancerres.aacrjournals.org/content/63/21/7158
Inhibition of Poly(ADP-Ribose) Polymerase Modulates Tumor-Related Gene Expression, Including Hypoxia-Inducible Factor-1...
... inhibitor of PARP, and applied simultaneously with 12-O-tetradecanoylphorbol-13-acetate (TPA). Control animals were also ... the levels of HIF-1α protein were determined in parp-1+/+ and parp-1−/− mice exposed to the inhibitor DPQ as described in ... New highly potent PARP inhibitors have shown their specificity and in vivo activity to enhance chemotherapy and radiotherapy of ... 33] and AP-1 (Tnc, procollagen type I, S100 family members, and Pold2; ref. 34), supporting our previous results that defective ...http://cancerres.aacrjournals.org/content/66/11/5744?ijkey=c60ce04f2abf13d6f520c0827926dda4eb6802ae&keytype2=tf_ipsecsha
Identification of zerumbone in Zingiber zerumbet Smith as a potent inhibitor of 12-O-tetradecanoylphorbol-13-acetate-induced ... 800 μg protein) was precipitated with antibody against IKKα followed by treatment with protein A/G-agarose beads. After 2 h of ... Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ... Finally, the protein was resolved on 10% SDS-PAGE, the gel was dried, and the radioactive bands were visualized with a ...http://cancerres.aacrjournals.org/content/69/4/1477
Molecular Characterization and Enhancement of Anticancer Activity of Caffeic Acid Phenethyl Ester by γ Cyclodextrin
... on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in mouse skin and the synthesis of DNA, RNA and protein in HeLa ... Caffeic acid phenethyl ester is a potent inhibitor of HIF prolyl hydroxylase: structural analysis and pharmacological ... Tumor protein p53 inducible nuclear protein 1, GATA3 (Gata binding protein 3) and RHOB (Ras homolog family member b) (Figs. 5A ... Molecular dynamics simulations as a tool for improving protein stability. Protein Eng. 2002;15:185-92 ...http://www.jcancer.org/v07p1755.htm
Celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," is a potent proteasome inhibitor and suppresses human ... O-tetradecanoylphorbol-13-acetate.. Constructs and inhibitors. An ATF2 peptide (amino acids 51-100) was cloned into HA-tagged ... Among the genetic changes commonly found in human melanomas are mutations in protein kinases of the mitogen-activated protein ... kinase (MAPK) family, indicating that signal transduction pathways have been largely modified in this tumor type (3, 4). ...http://clincancerres.aacrjournals.org/content/13/22/6769
Dietary chalcones with chemopreventive and chemotherapeutic potential | Genes & Nutrition | Full Text
Licochalcone A (18), another phytochemical isolated from licorice, belongs to a family of promising compounds with potent ... x-linked inhibitor of apoptosis protein (XIAP), and survivin. Flavokavain A (23) activated caspase 9, caspase 3, and poly (ADP- ... and mediated by 12-O-tetradecanoylphorbol-13-acetate (TPA). Isobavachalcone (15), isolated from Angelica keiskei, significantly ... Prenylation as protein post-translational modification results in higher protein lipophilicity and targets the modified protein ...https://genesandnutrition.biomedcentral.com/articles/10.1007/s12263-011-0210-5
Trefoil Factor 1 in Early Breast Carcinoma: A Potential Indicator of Clinical Outcome during the First 3 Years of Follow-Up
TPA: 12-0-tetradecanoylphorbol-13-acetate;. ER: Estrogen receptor;. AI: Aromatase inhibitor; ... A wide range of TFF1 levels was detected in breast carcinomas and protein levels varied from 1.5 to 135.8 ng/mg protein, with a ... 1. Thim L. A new family of growth factor-like peptides. 'Trefoil' disulphide loop structures as a common feature in breast ... that high levels of PR suppress tumor cell motility by preventing TFF1 dimerization as TFF1 dimer was shown to be more potent ...http://www.medsci.org/v11p0663.htm
Flavokawain A, a Novel Chalcone from Kava Extract, Induces Apoptosis in Bladder Cancer Cells by Involvement of Bax Protein...
Inhibitory effect of herbal remedies on 12-O-tetradecanoylphorbol-13-acetate-promoted Epstein-Barr virus early antigen ... Mountain View, CA). Caspase-3 inhibitor II (z-DEVD-fmk), caspase-9 inhibitor I (z-LEHD-fmk), and caspase inhibitor I (z-VAD-fmk ... Bcl-xL is the only Bcl-2 family protein specifically targeted to the mitochondrial outer membrane ( 40). The mitochondria ... Our findings should encourage not only the development of more potent chalcone derivatives for prevention and treatment of ...http://cancerres.aacrjournals.org/content/65/8/3479.long
Fruits Offer Powerful Protection From Skin Cancer
Our research has documented that perillyl alcohol is a potent in vivo (living cells) inhibitor of both UVB-induced non-melanoma ... 12-0-tetradecanoylphorbol-13-acetate), a strong promoter of chemically induced skin cancer. Applying pomegranate extract (2 mg/ ... and is a structurally unique member of the apoptosis inhibitors protein family. Survivin is overexpressed in most human cancers ... enzyme and protein levels; and protein levels of proliferating cell nuclear antigen (PCNA), all of which are established ...http://sci.rutgers.edu/forum/showthread.php?2622-Fruits-Offer-Powerful-Protection-From-Skin-Cancer&s=ad6cebac016e000fcdcb3485eef2a50c
The power and promise of "rewiring" the mitogen-activated protein kinase network in prostate cancer therapeutics | Molecular...
The Rho GTPase family affects various cellular processes like proliferation and apoptosis. Notably, the Rho kinase inhibitor Y- ... O-tetradecanoylphorbol-13-acetate response elements; ref. 24; Fig. 2). In addition, further to the classic nuclear "androgenic ... which was found to be a potent competitive inhibitor of ATP binding in the catalytic domains of Raf-1 and wild-type and mutated ... protein kinase C; RIN1, Ras and Rab interactor 1; RKIP, Raf kinase inhibitor protein; SOS, son of sevenless; TF, transcription ...http://mct.aacrjournals.org/content/6/3/811
Gene Expression sub-cluster 37
Furthermore, FPP-3 reduced MMP-2 expression at protein and mRNA levels, and suppressed 12-O-tetradecanoylphorbol-13-acetate ( ... Interaction of the Eph family of receptor protein tyrosine kinases and their ligands, ephrin family members, induces bi- ... The mRNA contents of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of MMP (TIMP)-1 and TIMP-2 in leukemia cells ... Expression and activity of above MMPs studied using gelatin zymography suggested SGlc as a potent ... ...http://www.biomedsearch.com/cluster/10/Gene-Expression/sub-37-p12.html
Clinical Application of Development of Nonantibiotic Macrolides That Correct Inflammation-Driven Immune Dysfunction in...
... biological response modifiers and the family of cell adhesion-promoting molecules. ... V. M. Rivera, R. M. Squillace, D. Miller et al., "Ridaforolimus (AP23573; MK-8669), a potent mtor inhibitor, has broad ... initiated and 12-tetradecanoylphorbol-13-acetate-(TPA-) promoted skin papillomas when 0.1 mol tacrolimus was applied topically ... Tacrolimus and the intracellular immunophilin protein known as the FK-binding protein form a complex that binds to and ...https://www.hindawi.com/journals/mi/2012/563709/
MK-1775, a potent Wee1 inhibitor, synergizes with gemcitabine to achieve tumor regressions, selectively in p53-deficient ... anthracene/12-O-tetradecanoylphorbol-l3-acetate) two-stage carcinogen protocol to induce SCC. Tumors induced by this protocol ... Tec protein-tyrosine kinase is an effector molecule of Lyn protein-tyrosine kinase. FASEB J 1996;10:637-42. ... rlk/TXK encodes two forms of a novel cysteine string tyrosine kinase activated by Src family kinases. Mol Cell Biol 1999;19: ...http://clincancerres.aacrjournals.org/content/20/16/4274
Immunology sub-cluster 65
Topical atorvastatin ameliorates 12-O-tetradecanoylphorbol-13-acetate induced skin inflammation by reducing cutaneous cytokine ... It has become clear that NETs are potent triggers of inflammation in autoimmune skin diseases. Moreover, the ability of NETs to ... Innate lymphoid cells (ILCs) are part of a heterogeneous family of innate immune cells with newly identified roles in mediating ... Atorvastatin is a 3-hydroxy-3-methylglutaryl coenzyme-A reductase inhibitor used in the treatment of atherosclerosis and ...http://www.biomedsearch.com/cluster/42/Immunology/sub-65.html
... on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in mouse skin and the synthesis of DNA, RNA and protein in HeLa ... We show here, for the first time, that CAPE is also a potent inhibitor of the NFAT pathway, and our results suggest that the ... diacylglycerol mediates activation of protein kinase C family members (Baier, 2003). As a consequence of an increase of ... Since CAPE has been described as a potent NF-κB inhibitor, and we have reported here that NFAT is also inhibited by CAPE, it is ...http://jpet.aspetjournals.org/content/308/3/993
Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway...
... which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation. Taken together, our data indicates that ... which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation. Moreover, EEPC clearly suppressed the ... Our results indicated that EEPC exhibits a potent inhibitory effect on NO production and inhibits PGE2 release in LPS-induced ... Suppression of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice by transduced Tat-Annexin protein. ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-101
Gene Expression sub-cluster 8
The neurite outgrowth inhibitor protein Nogo-A has been identified as an inhibitor of axonal regeneration, and Nogo-B as a ... In this study, we examined the effects of five selected food phytochemicals on 12-O-tetradecanoylphorbol-13-acetate (TPA)- ... The activator protein-1 (AP-1) family of transcription factors, including the most common member c-Jun-c-Fos, participates in ... Oxytocin (OT) is a potent uterine agonist. Its receptor (OTR) is a G protein-coupled receptor that is downregulated by ...http://www.biomedsearch.com/cluster/10/Gene-Expression/sub-8-p9.html
Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton | Journal of...
The diaphanous proteins are members of the ubiquitous formin protein family that can physically link the actin filament system ... Consistent with this assumption, we observed that microinjection of the ADP-ribosyltransferase C3, which is a potent inhibitor ... induced by the application of 12-O-tetradecanoylphorbol-13-acetate (TPA) (Watanabe et al., 1997), but the cells described here ... We demonstrate that complex formation between this mammalian protein with the actin-binding protein profilin is indeed mediated ...http://jcs.biologists.org/content/114/20/3663
The transcription factor Ap-1 regulates monkey 20α-hydroxysteroid dehydrogenase promoter activity in CHO cells | BMC...
These findings were confirmed by EMSA examining the interactions of the protein Ap-1 in a nuclear extract from CHO-K1 cells and ... When the reporter constructs were co-transfected with Ap-1 (Jun) and specificity protein (Sp-1) genes, the transcription ... revealed that a region corresponding to the activator protein 1 (Ap-1) located between -281 and -274 bp was essential for the ... such as the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) . Jun homodimers have less DNA binding activity than ...https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-14-71
A Live-Cell Assay for Studying Extracellular and Intracellular Endothelin-Converting Enzyme Activity | Hypertension
The effects of thiorphan, a specific NEP inhibitor,14 and phosphoramidon, an ECE inhibitor,31 were tested on the conversion of ... 12-o-tetradecanoyl-phorbol-13-acetate- responsive element. Acknowledgments. This work was supported in part by the Ministère de ... Endothelin induces transcription of fos/jun family genes: a prominent role for calcium ion. Mol Endocrinol. 1992;6:1003-1012. ... SB209670, a rationally designed potent nonpetide endothelin receptor antagonist. Proc Natl Acad Sci U S A. 1994;91:8052-8056. ...http://hyper.ahajournals.org/content/30/4/837
We have adopted a special experimental strategy to identify early responsive genes during 12-O-tetradecanoylphorbol-13-acetate ... performed in cells that were synchronized by nocodazole and treated with TPA in the presence of a protein synthesis inhibitor, ... Multigene families encoding surface antigens demonstrate an exclusive expression of proteins. The resulting presence of just ... This may explain why DEDD L is a more potent apoptosis inducer, because DED-containing proteins usually induce apoptosis ...https://www.cognizantcommunication.com/cccSiteFiles/Gene/gene13abs3.html
WikiGenes - SDC1 - syndecan 1
Physiological degradation converts the soluble syndecan-1 ectodomain from an inhibitor to a potent activator of FGF-2. Kato, M ... In contrast, syndecan-1 shedding induced by 12-O-tetradecanoylphorbol-13-acetate treatment was inhibited by BB-94 but not by ... Human CASK/LIN-2 binds syndecan-2 and protein 4.1 and localizes to the basolateral membrane of epithelial cells. Cohen, A.R., ... The syndecan family of cell-surface heparan sulfate proteoglycans participate in multiple cell behaviors ranging from growth ...https://www.wikigenes.org/e/gene/e/6382.html
Growth factors can activate ATF2 via a two‐step mechanism: phosphorylation of Thr71 through the Ras-MEK-ERK pathway and of...
... tetradecanoylphorbol‐13‐acetate (TPA) to examine further the role of the Raf-MEK pathway in ATF2 activation. TPA is a potent ... A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein. ... As the Src inhibitor PP1 had the same selective effects on ATF2 Thr69 + 71 dual phosphorylation as the p38 inhibitor SB203580 ( ... Reuther GW and Der CJ (2000) The Ras branch of small GTPases: Ras family members don't fall far from the tree. Curr Opin Cell ...http://emboj.embopress.org/content/21/14/3782
Noni (Morinda citrifolia) | Plant Profiler | China-Mainland | Sigma-Aldrich
... cell transformation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) or epidermal growth factor (EGF)33,34 , the tumor- ... 6-trihydroxyanthraquinone may be a potent quinine reducatase inducer that is nearly 40 times more potent than the positive ... A new unusual iridoid with inhibition of activator protein-1 (AP-1) from the leaves of Morinda citrifolia L. Org Lett 5-3-2001; ... family), rubiadin, rubiadin-1-methyl ether, rutin, scopoletin, te non (Gilbertese), terpenoids, TNJ, ursolic acid, vitamin A, ...https://www.sigmaaldrich.com/china-mainland/zh/life-science/nutrition-research/learning-center/plant-profiler/morinda-citrifolia.html