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*  YAC Transfer into Mammalian Cells by Cell Fusion | Springer for Research & Development
... are ideal for expression studies of the complex genes and gene clusters found in the mammalian genome. Such studies... ... The large regions of DNA that can be cloned in yeast artificial chromosomes (YACs) ... 1991) Stable integration and expression in mouse cells of yeast artificial chromosomes harboring human genes. Proc. Natl. Acad ... Huxley, C. and Gnirke, A. (1991) Transfer of yeast artificial chromosomes from yeast to mammalian cells. BioEssays 13, 545-549. ...
  https://rd.springer.com/protocol/10.1385/0-89603-313-9%3A281
*  Human antibodies derived from immunized xenomice - Abgenix, Inc.
Eliceiri, et al., "Stable integration and expression in mouse cells of yeast artificial chromosomes harboring human genes," ... Garza, et al., "Mapping the drosophila genome with yeast artificial chromosomes with yeast artificial chromosomes", Science 246 ... Gnirke, et al., "Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes", EMBO Journal 10(7): ... YEAST ARTIFICIAL CHROMOSOMES AND THEIR USE IN THE CONTROL OF GENE EXPRESSION. ...
  http://www.freepatentsonline.com/6150584.html
*  Artificial chromosome - Patent # 6716608 - PatentGenius
... preparing a CEPH artificial yeast chromosome library containing a human genome, identifying clones having a repetitive human ... An artificial mammalian chromosome, more specifically, a clone containing a mammalian centromere sequence and a DNA replication ... alphoid sequence from this library, and further preparing a yeast strain in which mammalian telomere sequences are added to the ... Peterson et al., "Use Of Yeast Artificial Chromosomes (YACs) For Studying Control Of Gene Expression: Correct Regulation Of The ...
  http://www.patentgenius.com/patent/6716608.html
*  Transgenesis upgrades for Drosophila melanogaster | Development
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425,917 -925. ... Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells. Genomics 83,1072 -1082. ... Pioneered in the yeast Schizosaccharomyces pombe (Thomason et al., 2001) and in mouse (Belteki et al., 2003), a modification of ... bacterial artificial chromosomes (BACs) (Shizuya et al., 1992) and P1 artificial chromosomes (PACs) (Ioannou et al., 1994), ...
  http://dev.biologists.org/content/134/20/3571
*  Nucleic acid sequences encoding strictosidine synthase proteins - Patent # 8710201 - PatentGenius
Bradshaw et al., "A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes" Nucl. ... "Expression of genes transferred into monocot and dicot plant cells by electroporation" Proc. Natl. Acad. Sci. USA, 82:5824 ( ... "Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast" Proc. Natl. Acad. Sci. ... Burke et al., "Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors" Science, 236: ...
  http://www.patentgenius.com/patent/8710201.html
*  Craig Cameron Mello | Encyclopedia.com
... working with yeast had identified functional DNA elements that direct the replication and partitioning of chromosomes. ... Mello was intrigued that bacterial cells could speak the same genetic language as human cells to produce a human protein useful ... understanding these essential functional chromosomal elements and then using them to produce stable artificial chromosomes for ... More specifically, in the technique, dsRNA triggers sequence-specific silencing of (or interfering with) gene expression, ...
  https://www.encyclopedia.com/people/science-and-technology/genetics-and-genetic-engineering-biographies/craig-cameron-mello
*  Xenotransplantation - the transplant of organs or tissues between different species has become a major issue within the past
... to use whole yeast artificial chromosomes (YACs) containing large segments of the human genome to optimize gene expression, but ... But high expression did not guarantee expression in endothelial cells (cells on the surfaces of the organ). No correlation was ... The use of artificial organs and human cells and tissues will both avoid the risk of cross-species viral epidemics. ... And, it had not performed FDA-required tests to determine whether its mouse cells could infect human cells. Most shocking was ...
  https://www.iatp.org/files/Xenotransplantation_How_Bad_Science_and_Big_Bu.htm
*  PDCD2 | Cancer Genetics Web
A microarray based expression profiling of paclitaxel and vincristine resistant MCF-7 cells.. Eur J Pharmacol. 2011; 657(1-3):4 ... In all cases, deletions of 6q27 extended from yeast artificial chromosome (YAC) 977e10 spanning the proximal border of RMD1 to ... Genes Chromosomes Cancer. 2003; 37(4):421-6 [PubMed] Related Publications Deletions in the long arm of chromosome 6 (6q) are ... We found that in primary mouse B cells, BCL-6 repressed expression of the Blimp-1, p27kip1, and cyclin D2 target genes. BCL-6 ...
  http://www.cancerindex.org/geneweb/PDCD2.htm
*  Polynucleotides Encoding Stop Codons in Multiple Reading Frames and Methods of Use - PIONEER HI-BRED INTERNATIONAL, INC.
The compositions include isolated polynucleotides, expression cassettes, a ... from yeast episomes, from yeast chromosomal elements, including yeast artificial chromosomes; from viruses, such as, ... Baculovirus vectors available for expression of proteins in cultured insect cells, such as, Sf9 cells, include the pAc series ( ... mini-chromosomes, artificial chromosomes, satellite chromosomes and the like; vectors derived from bacterial plasmids, from ...
  http://www.freepatentsonline.com/y2009/0038034.html
*  METHODS AND COMPOSITIONS FOR RNA-GUIDED TREATMENT OF HIV INFECTION - TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER...
... artificial chromosomes, BACs, YACs, or PACs. The term "vector" includes cloning and expression vectors, as well as viral ... yeast plasmids such as the 2μ plasmid or derivatives thereof, vectors useful in eukaryotic cells, such as vectors useful in ... Embryonic stem cells (ES cells) and artificial pluripotent stem cells (induced pluripotent stem cell, iPS cells) have been ... natural killer cells, dendritic cells such as Langerhans cells and follicular dendritic cells, hematopoietic stem cells, ...
  http://www.freepatentsonline.com/y2016/0250300.html
*  Plus it
3 The abbreviations used are: FISH, fluorescence in situ hybridization; YAC, yeast artificial chromosome. ... In the vast majority of cells, the 9p rearrangement was detectable in one homologue. Only a fraction of cells appears to have ... Pdef Expression in Human Breast Cancer Is Correlated with Invasive Potential and Altered Gene Expression ... 1F ⇓ , c) or translocations of distal 9p to other chromosomes (Fig. 1F ⇓ , d) were observed. Collectively these results ...
  http://cancerres.aacrjournals.org/content/61/13/5179
*  Human Genome Project | Encyclopedia.com
A new artificial chromosome vector called YAC (yeast artificial chromosome) was introduced providing a construct with an even ... chromosomes), in the centers of cells, and that these are not only the units of function-the things that carry the information ... "The I.M.A.G.E. Consortium: An Integrated Molecular Analysis of Genomes and Their Expression." Genomics 33 (1996): 1512. ... A new artificial chromosome vector called YAC (yeast artificial chromosome) was introduced providing a construct with an even ...
  https://www.encyclopedia.com/science-and-technology/biology-and-genetics/genetics-and-genetic-engineering/human-genome-project
*  WO2003025137A2 - Sweet taste receptors - Google Patents
Common vectors include plasmids, viral genomes, and (primarily in yeast and bacteria) 'artificial chromosomes.' 'Expression ... Streptomyces and Bacillus subtilis cells; fungal cells such as yeast cells, e.g., Pichia, and Aspergillus cells; insect cells ... for the identification of sweet or glutamate tastants in cells. The cells that are rescued can include cells in culture, ( ... human taste cells or heterologous cells expressing hTIRs, or plasma membrane preparations of such cells, can be utilized to ...
  https://patents.google.com/patent/WO2003025137A2/en
*  Type 2 cytokine receptor and nucleic acids encoding same - Patent application
... insect cells (using baculovirus expression vectors) yeast cells or mammalian cells. Suitable host cells are discussed further ... The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. ... T cells, B cells, NK cells, NK T cells, dendritic cells, macrophages, monocytes, neutrophils, mast cells, basophils, ... yeast or mammalian cells (such as human, Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known ...
  http://www.patentsencyclopedia.com/app/20090232809
*  Indian Patents. 233827:'A PROCESS FOR RECOVERING POLYPEPTIDE, AN IMMUNOGLOBIN VARIABLE DOMAIN THAT UNFOLDS REVERSIBLY FROM A...
... artificial chromosomes and episomal vectors. Such vectors may be used for simple cloning and mutagenesis, or an expression ... such as prokaryotic cells (E. coif), insect cells (Drosophila Schnieder S2 cells, Sf9) and yeast (P. methcmolica, P. pastoris, ... Suitable expression vectors for expression in prokaryotic (e.g., bacterial cells such as E. coli) or mammalian cells include, ... Expression constructs or expression vectors suitable for the expression of a antibody or antigen-binding fragment are also ...
  http://www.allindianpatents.com/patents/233827-a-process-for-recovering-polypeptide-an-immunoglobin-variable-domain-that-unfolds-reversibly-from-a-repertoire-of-polypeptides
*  Yeast artificial chromosome - Wikipedia
With the insertion of a YAC into yeast cells, they can be propagated as linear artificial chromosomes, cloning the inserted ... Yeast expression vectors, such as YACs, YIps (yeast integrating plasmids), and YEps (yeast episomal plasmids), have an ... Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces ... Yeast Artificial Chromosomes at the US National Library of Medicine Medical Subject Headings (MeSH) North Dakota State ...
  https://en.wikipedia.org/wiki/Yeast_artificial_chromosome
*  FLP-FRT recombination - Wikipedia
The novel GRIM Expression System allows for the much faster generation of expression vectors containing artificial RNAi ... The authors went on to show that their expression system works quite effectively in human embryonic kidneys (HEK) cells, a ... This has been seen from fruit flies to mice and is indiscriminate against specific chromosomes (somatic and sex) or cell types ... Senecoff, Julie F.; Rossmeissl, Peter J.; Cox, Michael M. (1988-05-20). "DNA recognition by the FLP recombinase of the yeast 2 ...
  https://en.wikipedia.org/wiki/FLP-FRT_recombination
*  Patente US8093043 - β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing ... - Google Patentes
... the invention relates to inhibitors of at least one of β-TrCP1/2 or RSK1/2 proteins that sensitize tumor cells to chemotherapy- ... to sensitize cancer cells to cell death or apoptosis. In certain embodiments, the invention relates to increasing BimEL levels ... and yeast artificial chromosomes.. Expression constructs of the present invention may comprise vector sequences that facilitate ... cells were seeded at 5 105 cells/well, and left in medium containing fetal calf serum (FCS). On Day 2, cells were re-feed cells ...
  http://www.google.es/patents/US8093043?hl=es&dq=flatulence
*  Patent US7981863 - Treatment of Parkinson's disease with PDGF - Google Patents
... differentiation and survival of central nervous system cells. These methods are useful for reducing at least one symptom of the ... The invention relates generally to methods of influencing central nervous system cells to produce progeny useful in the ... The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. ... Using RT-PCR, the MRNA from these cells was examined for the expression of 4 different isoforms of VEGF. GADPH expression ...
  http://www.google.com/patents/US7981863?dq=7,134,016
*  Robin Allshire - Wikipedia
"A fission yeast chromosome can replicate autonomously in mouse cells". Cell. 50: 391. 1987. PMID 3475186. "Introduction of ... "Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast". J Cell Sci ... "Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes". EMBO J. 13: 3801 ... guidance of Chris Bostock and Ed Southern investigating the use of Bovine Papilloma Virus as a chassis for mammalian artificial ...
  https://en.wikipedia.org/wiki/Robin_Allshire
*  Transgenic non-human animals capable of producing heterologous antibodies of various isotypes - GenPharm International Inc.
Murray & Szostak, Construction of artificial chromosomes in yeast, Nature 305:189-193 (1983). Neuberger et al., Isotype ... This construct expresses an RNA transcript in B-cells that hybridizes with mouse IgM mRNA, thus down-regulating the expression ... and IgG3 expression and increases IgE and IgG1 expression; while IFNγ selectively stimulates IgG2a expression and antagonizes ... 4) are pooled and cloned into the NotI site of the vector PYACNN in Yeast cells. Plasmid pYACNN is prepared by digestion of ...
  http://www.freepatentsonline.com/5877397.html
*  Patent US8170808 - Methods and computer software for detecting splice variants - Google Patents
In general the methods involve normalizing probe set or exon intensity to an expression level measurement of the gene. The ... Design of artificial genes for use as controls in gene expression analysis systems. ... I-IV), Using Antibodies: A Laboratory Manual, Cells: A Laboratory Manual, PCR Primer: A Laboratory Manual, and Molecular ... The term "genome" as used herein is all the genetic material in the chromosomes of an organism. DNA derived from the genetic ...
  http://www.google.com/patents/US8170808?dq=patent:7076806
*  PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants. - Semantic Scholar
The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase ... Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping.. *Heinz-Ulrich G Weier, ... NF45/ILF2 tissue expression, promoter analysis, and interleukin-2 transactivating function.. *Guohua Zhao, Lingfang Shi, ... Carrier-specific breakpoint-spanning DNA probes: an approach to preimplantation genetic diagnosis in interphase cells.. *M J ...
  https://www.semanticscholar.org/paper/PCR-probes-for-chromosome-in-situ-hybridization-of-Kroisel-Ioannou/9ccc3142e29f4aa0a29ad91da061a3e315589a00
*  Difference between revisions of "User:Jarle Pahr/Articles" - OpenWetWare
Artificial repressors for controlling gene expression in bacteria: http://pubs.rsc.org/en/content/articlepdf/2013/cc/c2cc37107c ... Transferring a synthetic gene circuit from yeast to mammalian cells: http://www.nature.com/ncomms/journal/v4/n2/full/ncomms2471 ... Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement 2012. A novel PCR-based method for high ... Per co-transformation 200 ng of vector plus 50-500 ng of insert were mixed and the competent cells added to the DNA mixture ...
  https://openwetware.org/wiki/?title=User:Jarle_Pahr/Articles&curid=130354&diff=685253&oldid=684404
*  Genes and Hypertension | Hypertension
... physical mapping of the region is undertaken with yeast artificial chromosomes, bacterial artificial chromosomes, and a P1 ... They studied differential expression between the parental SHR and the SHR.4 strain. These 2 strains are identical apart from ... Overexpression of human superoxide dismutase inhibits oxidation of low-density lipoprotein by endothelial cells. Circ Res. 1998 ... then the flanking yeast artificial chromosomes clones will overlap, as judged by pulsed field electrophoresis restriction ...
  http://hyper.ahajournals.org/content/35/1/164