*  Events - World Nuclear Association
World Nuclear Association Symposium, WNFC (World Nuclear Fuel Cycle) and other conference and events from the World Nuclear Association
*  A) Intranuclear localization of U2-C/D motif chimeras. | Open-i
(A) Intranuclear localization of U2-C/D motif chimeras. 1 fmol of fluorescein-labeled wild-type U2-C/D motif chimera (U2+CD), Sm-mutant U2-C/D motif chimera (U2
*  Import Export Accounting Jobs In India | Import Export Accounting Jobs | Timesjobs.com
Import Export Accounting Jobs in India. Apply for Import Export Accounting Jobs. Employment Opportunities for Import Export Accounting in accounting at Timesjobs.com
*  Russian military uses nanotechnology to build world's most powerful non-nuclear bomb
The Russian military has successfully tested what it described as the world's most powerful non-nuclear air-delivered bomb, Russia's state television reported Tuesday.
*  Margulis, Mol Vis 2002; 8:477-482. Figure 5.
Figure 5. Estimation of rhodopsin and phosducin in retinal homogenate and purified nuclear preparation. Proteins of the retinal homogenate and purified nuclear preparation were subjected to electrophoresis, transferred to PVDF membrane and probed with anti-phosducin (left panel) or anti-rhodopsin (right panel) antibody. A: 100 ng of phosducin standard; B: 100 ng of rhodopsin standard. Numbers under lanes represent the amount of total protein loaded, in μg.. ...
*  Examination of determinants for intranuclear localization and transactivation within the RING finger of herpes simplex virus...
*  Plus it
In previous reports we challenged the concept that uterine nuclei of rats contain two forms of estrogen receptors, one salt extractable and the other salt resistant. Although it is likely that a certain fraction of the nuclear bound receptor-estrogen complex exists as a ternary high-affinity acceptor-receptor-estrogen complex, current salt extraction procedures do not allow discrimination between receptor-17β-estradiol complexes associated with high-affinity and low-affinity nuclear binding sites. Recent reports suggested that the DNA-intercalating agent ethidium bromide selectively extracted those sites that appeared to be salt resistant. In view of contradictory reports to this effect, we have attempted to clarify this issue. The data presented indicate that ethidium bromide is not a useful tool for the identification of a specific class ("salt-resistant") of nuclear binding sites for receptor-17β-estradiol complexes. This conclusion is based on measurement of nuclear bound ...
*  Press releases - Article | Friedrich Miescher Institute
Using the genetically amenable model organism C. elegans, a small worm commonly found on rotting fruits, FMI scientists have shown that the driving force for gene localization is encoded in the DNA sequence of promoters. Cell type-specific developmentally regulated promoters direct genes either to the nuclear interior when they are active or towards the compacted chromatin at the nuclear periphery when inactive. In muscle differentiation this is controlled by the presence of a "master regulatory" transcription factor called Hlh-1 (MyoD in mammals). Specific localization is not seen in committed embryonic cells nor for housekeeping genes. The authors find a dramatic increase in nuclear compartmentalization during the course of development and cell differentiation. This study opens the way to genetic analysis of nuclear organization and will allow the analysis of human diseases linked to nuclear function using worms as models ...
*  In leukemia, discovery of Mer protein in cancer cells' nuclei offers another place to target this known cause of cancer
Migdall and Graham think it's likely that Mer in the nucleus may influence "gene expression" - helping to decide which parts of the cells' DNA are printed or expressed into proteins. If Mer is, in fact, altering genes within cells, it may be one way in which healthy cells become cancerous - with the wrong genes expressed, a good cell may go bad. Or perhaps Mer in the nucleus may help existing cancer cells survive and thrive despite chemotherapy treatment, as is commonly the case in patients who relapse ...
*  Cancer cell nucleus, TEM - Stock Image C019/9935 - Science Photo Library
Cancer cell nucleus. Coloured transmission electron micrograph (TEM) of a deformed nucleus (yellow) in a cancer cell. Healthy nuclei have a smooth membrane, that is spherical or ovoid, whereas this nucleus has indentations in several places. The nucleus contains the cell's genetic information. Within the nucleus is the nucleolus (brown), which is responsible for producing components of ribosomes, the cell's protein-manufacturing organelles. Magnification: x6000 when printed at 10 centimetres wide. - Stock Image C019/9935
*  Cellular differentiation state modulates the mRNA export activity of SR proteins | JCB
Although several SR proteins were reported to shuttle poorly in HeLa cells (Cáceres et al., 1998; Lin et al., 2005; Sapra et al., 2009), we have recently shown that all SR proteins act as NXF1 adapters in pluripotent P19 cells (Müller-McNicoll et al., 2016). To investigate this discrepancy, we developed a quantitative shuttling assay to measure the nucleocytoplasmic shuttling of seven canonical family members. Key technical advances were the use of stable clonal cell lines expressing similar and near-endogenous levels of GFP-tagged proteins (donor) and a membrane-bound marker protein (recipient). Quantification of total nuclear fluorescence in a large number of donor and recipient cells allowed for the first time the determination of mean shuttling capacities of individual SR proteins. We could show that all seven SR proteins shuttle in P19 cells; however, they shuttle to different extents, suggesting a differential ...
*  Most recent papers with the keyword Cell of origin | Read by QxMD
In the interphase cell nucleus, chromosomes adopt a conserved and non-random arrangement in subnuclear domains called chromosome territories (CTs). Whereas chromosome translocation can affect CT organization in tumor cell nuclei, little is known about how aneuploidies can impact CT organization. Here, we performed 3D-FISH on control and trisomic 21 nuclei to track the patterning of chromosome territories, focusing on the radial distribution of trisomic HSA21 as well as 11 disomic chromosomes. We have established an experimental design based on cultured chorionic villus cells which keep their original mesenchymal features including a characteristic ellipsoid nuclear morphology and a radial CT distribution that correlates with chromosome size ...
*  How to build a cell nucleus: it starts from the ends
The spatial and temporal organization of the cell nucleus and its components is essential for genome regulation. The nuclear envelope maintains the shape and the mechanical integrity of the nucleus, but also provides an anchor for chromatin that is connected to its inner side. This interaction mediates the regulation of gene expression, DNA replication and the maintenance of genome stability. Cell nuclei need to be rebuilt after each cell division, and this is achieved in a remarkable short period of time. My lab use state-of-the art microscopy (live and super-resolution), as well as complementary approaches, to understand how human telomeres, natural ends of linear chromosomes, play an essential role in this critical process.
*  Saylor.org's Cell Biology/The Cell Nucleus and Gene Expression - Wikibooks, open books for an open world
As you learned in BIO101, the cell nucleus is the storage area for all genetic material and constantly full of activity. The nucleus in fact contains not only DNA, but RNA and protein as well. This unit will take a detailed look at chromosomes, the cell nucleus, gene expression, and expression regulation. When we refer to "expression regulation," we are talking about the fact that not all genes are expressed in the cell at the same time. After all, though a liver cell and a nerve cell have the same genome (and thus the same DNA), they look and act completely differently. How does this happen? The answer is regulated gene expression!. ...
*  The Role of Cell Nucleus | Custom Writing Help Site
Molecular Expressions Cell Biology: The Cell Nucleus Function of the Nucleus. and the nucleoskeleton which nuclei the role as a whole. The nucleus maintains the security
*  "Intranuclear trafficking: organization and assembly of regulatory mach" by Sayyed K. Zaidi, Daniel W. Young et al.
The molecular logistics of nuclear regulatory processes necessitate temporal and spatial regulation of protein-protein and protein-DNA interactions in response to physiological cues. Biochemical, in situ, and in vivo genetic evidence demonstrates the requirement for intranuclear localization of regulatory complexes that functionally couple cellular responses to signals that mediate combinatorial control of gene expression. We have summarized evidence that subnuclear targeting of transcription factors mechanistically links gene expression with architectural organization and assembly of nuclear regulatory machinery for biological control. The compromised intranuclear targeting of regulatory proteins under pathological conditions provides options for the diagnosis and treatment of disease.
*  intact nuclei from tissue samples?
Dear all, I am searching for a method that will allow me to isolate intact nuclei from rat brain tissue (we aren't tooled up for cell culture right now - otherwise I would use one of the many techniques available for such a situation). Many thanks. -- _____________________________________________________________________ Keith Hoek hoek at biosci.uq.edu.au mRNA transport http://florey.biosci.uq.edu.au/~hoek/Pg1.html ...
*  Quantifying colocalization : thresholding, void voxels and the H-coef
A critical step in the analysis of images is identifying the area of interest e.g. nuclei. When the nuclei are brighter than the remainder of the image an intensity can be chosen to identify the nuclei. Intensity thresholding is complicated by variations in the intensity of individual nuclei and their intensity relative to their surroundings. To compensate thresholds can be based on local rather than global intensities. By testing local thresholding methods we found that the local mean performed poorly while the Phansalkar method and a new method based on identifying the local background were superior. A new colocalization coefficient, the Hcoef, highlights a number of controversial issues. (i) Are molecular interactions measurable (ii) whether to include voxels without fluorophores in calculations, and (iii) the meaning of negative correlations. Negative correlations can arise biologically (a) because the two fluorophores are in different places or (b) when high intensities of one fluorophore ...
*  Scientists find signals that make cell nucleus blow up like a balloon | EurekAlert! Science News
The size of a cell's nucleus varies from one species to another, in different cell types and at different stages of development, and even with disease: many cancer cells develop larger nuclei as they become more malignant. Working with the African clawed frog, Rebecca Heald and Daniel Levy of University of California -- Berkeley have discovered two proteins that control the size of the nucleus. One imports lamin to shore up the nuclear envelope; the other controls importation.
*  Biology-Online • View topic - Activation Energy and Enzyme Catalysis
The progress of a reaction can be graphed 2-dimensionally as a reaction coordinate vs potential energy. Everyone has seen these potential energy profiles. From what I understand, the profile for a reaction is derived in this way: The potential energy of a set of nuclei can be graphed as dependent variable on a hyperdimensional surface. The extra dimensions include different states of the nuclei (vibrational rotational etc.)and their positions with respect to each other. If all but 2 dimensions can be set as constant, then those 2 independent variables (usually representing internuclear distance?) can be graphed with respect to the dependent variable of potential energy. The low points on this surface represent reactants, products, or intermediates. The saddle points represent transition states. The lowest energy path (the gradient) from reactant to transition state to product is the potential energy profile, which is graphed against a reaction coordinate ...
*  De Histology: Introduction of The Cell Nucleus
The nucleus contains a blueprint for all cell structures and activities, encoded in the DNA of the chromosomes. It also contains the molecular machinery to replicate its DNA and to synthesize and process the three types of RNA : ribosomal (rRNA), messenger (mRNA), and transfer (tRNA). Mitochondria have a small DNA genome and produce RNAs to be used in this organelle, but the genome is so small that it is not sufficient even for the mitochondrion itself. On the other hand, the nucleus does not produce proteins; the numerous protein molecules needed for the activities of the nucleus are imported from the cytoplasm ...
*  Journey to the Center of the Cell | Science
The animal cell nucleus houses the genetic material of the organism and therefore protects and maintains the blueprint for the cell and all its progeny. However, the nucleus is more than a simple repository for chromosomes. A dynamic organelle, the nucleus goes through astonishing transformations during each cell cycle, breaking down completely during mitosis and reforming afresh in each daughter cell after cell division. Within the nucleus, chromosomes are replicated and their DNA is transcribed to provide information that programs the physiology of the cell. Also, ribosomes assemble in the nucleus, then leave and carry out protein translation in the cytoplasm. All of this activity requires complex machineries that can respond to the changing needs of the cell throughout the cell cycle and ...
*  Genome Organization And Function In The Cell Nucleus, Buch. Karsten Rippe - ReadRate
Genome Organization And Function In The Cell Nucleus von Karsten Rippe und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
*  A Comprehensive Guide to Cell Nucleus History and Histology
The cell, with its chromatin, ribosomes, and organelles is a complex command center for the proper functioning of the cell. The cell nucleus also contains the genetic material necessary for the identification and replication of life.
*  About Stereology
There are several types of stereology, but instead of talking about all of these (which is the subject of books) this page will get you started with the most common and simplest form of stereology: 'Point Counting Stereology'. In point counting stereology you typically project a uniform grid of points over an image, and then simply count how many points fall inside the particular compartments you're interested in quantifying the volume of (eg: Mitochondria, Nucleus). After counting ~1000 points (which should take under one hour) you can make estimates such as the 'fraction of non-nuclear cell volume occupied by mitochondria' for a wild-type mouse (as averaged over a large area). In biology, a good stereologists would repeat this for three wild-type mice, and then three mutant mice... and at that stage the results should have enough accuracy to compare the conditions with good statistical accuracy and publish the results - results which may either support or reject the ...