The Calcium Sensitivity of Individual Secretory Vesicles Is Invariant with the Rate of Calcium Delivery | JGP
Exocytosis occurs when a vesicle has at least one fusion complex active; the rate of exocytosis is a function of the number of ... Elimination of Models for Calcium-triggered Exocytosis. The properties of sea urchin egg secretory vesicle exocytosis in vitro ... 1987) Exocytosis from the vesicle viewpoint: an overview. Ann NY Acad Sci 493:504-523, pmid:3109302.. ... 1994) Using caged calcium to study sea urchin egg cortical granule exocytosis in vitro. Methods (Orlando) 6:82-92. ...http://jgp.rupress.org/content/112/5/569
GTP Hydrolysis of TC10 Promotes Neurite Outgrowth through Exocytic Fusion of Rab11- and L1-Containing Vesicles by Releasing...
The use of exocytosis for membrane expansion at nerve growth cones is critical for neurite outgrowth. TC10 is a Rho family ... Exocytosis of Rab11- and L1-positive vesicles may play a central role in TC10-mediated neurite outgrowth. The combination of ... Therefore, activity of TC10 at vesicles is presumed to be inactivated near the plasma membrane during neuronal exocytosis. Our ... exocytosis in HeLa cells suggests that the signaling machinery containing TC10 proposed here may be broadly used for exocytosis ...http://books.duhnnae.com/2017/jun8/149831306945-GTP-Hydrolysis-of-TC10-Promotes-Neurite-Outgrowth-through-Exocytic-Fusion-of-Rab11-and-L1-Containing-Vesicles-by-Releasing-Exocyst-Component-Exo70.php
Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close...
This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that ... Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ ... or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. ... and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated ...https://www.rdm.ox.ac.uk/publications/3525
Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed...
Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed- ... constitutive exocytosis; membrane fusion; Munc18b; soluble N-ethylmaleimide-sensitive factor-attachment receptor (SNARE); ...https://www.research-collection.ethz.ch/handle/20.500.11850/158675
To better understand how the proteins mediating exocytosis operate, the reconstruction of exocytosis in a "naive" cell is ... Reconstitution of exocytosis using synthetic vesicles in intact cells. Institution: Department of Neurobiology, Max-Planck- ... Jahn's team plans to reconstruct regulated, neuron-like exocytosis by testing the hypothesis as to whether it suffices to equip ... Upon arrival of an action potential within a nerve terminal, voltage-gated calcium channels open and trigger exocytosis of ...http://www.balzan.org/en/prizewinners/reinhard--jahn/research-project-jahn
Spatiotemporal Resolution of Mast Cell Granule Exocytosis | Journal of Cell Science
Spatiotemporal Resolution of Mast Cell Granule Exocytosis Message Subject (Your Name) has sent you a message from Journal of ... Mast cell activation initiated by antigen-mediated crosslinking of IgE receptors results in stimulated exocytosis of secretory ... and compound exocytosis. Using this method, we find strong dependence of degranulation kinetics on the duration of cell- ... dextran as a reporter for granule exocytosis in RBL mast cells and takes advantage of the pH sensitivity of FITC. We ...http://jcs.biologists.org/content/early/2012/03/02/jcs.102632
Exocytosis elicited by action potentials and voltage-clamp calcium currents in individual mouse pancreatic B-cells. -...
2. Exocytosis was dependent on a rise in [Ca2+]i and could be evoked by activation of voltage-dependent Ca2+ currents. The ... 4. Exocytosis could be initiated by simulated action potentials. Whereas a single action potential only produced a small ... 8. Over short periods, exocytosis may be extremely fast (1 pF/s or 500 granules/s), which is much higher than the rate of ... 3. During repetitive stimulation (1 Hz), when [Ca2+]i attained micromolar levels, exocytosis also took place during the ...https://www.dpag.ox.ac.uk/publications/20040
Regulation of Patterned Dynamics of Local Exocytosis in Growth Cones by Netrin-1 | Journal of Neuroscience
Netrin-1 elicits exocytosis in growth cones by increasing peak dynamics. To study the regulation of growth cone exocytosis by ... including the exocytosis of single vesicles and in bulk exocytosis of small vesicles or other vesicular components (Hines et al ... growth cone exocytosis takes place as spontaneous patterned peaks of increased exocytosis, followed by periods of low membrane ... 2010) Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines. Cell 141:524-535, doi:10.1016/j.cell. ...http://www.jneurosci.org/content/35/13/5156
Calcium influx and signaling in cytotoxic T-lymphocyte lytic granule exocytosis - Pores-Fernando - 2009 - Immunological Reviews...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...http://onlinelibrary.wiley.com/doi/10.1111/j.1600-065X.2009.00809.x/pdf
Kv2.1 Clustering Contributes to Insulin Exocytosis and Rescues Human β-Cell Dysfunction | Diabetes
Insulin exocytosis is regulated by ion channels that control excitability and Ca2+ influx. Channels also play an increasingly ... Thus, the ability of Kv2.1 to directly facilitate insulin exocytosis depends on channel clustering. Loss of this structural ... Kv2.1 Clustering Contributes to Insulin Exocytosis and Rescues Human β-Cell Dysfunction ... Kv2.1 Clustering Contributes to Insulin Exocytosis and Rescues Human β-Cell Dysfunction ...http://diabetes.diabetesjournals.org/content/early/2017/05/31/db16-1170
Thrombin-induced lysosomal exocytosis in human platelets is dependent on secondary activation by ADP and regulated by...
Exocytosis of lysosomal contents from platelets has been speculated to participate in clearance of thrombi and vessel wall ... Thrombin-induced lysosomal exocytosis in human platelets is dependent on secondary activation by ADP and regulated by ... Notably, lysosomal exocytosis in response to thrombin was significantly reduced if the secondary activation by ADP was ... The mechanisms that regulate lysosomal exocytosis in platelets are, however, still unclear. The aim of this study was to ...http://oru.diva-portal.org/smash/record.jsf?pid=diva2:906036
VCAC: Cellular Processes: Regulated Secretion (Golgi): Advanced Look: Exocytosis
Regulated Secretion: Advanced Look --, 2.) Exocytosis In regulated secretion, the exocytosis process is not completed until a ... Exocytosis is completed as the fusion of the secretory vesicles into the cell membrane releases insulin into neighboring blood ... HOME , REGULATED SECRETION , ADVANCED LOOK , 1.) VESICLES , 2.) EXOCYTOSIS. ...http://vcell.ndsu.nodak.edu/animations/regulatedsecretion/exocytosis_02.htm
Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells. - Radcliffe Department of Medicine
Experiments involving photorelease of caged Ca(2+) revealed that the rate of exocytosis was half-maximal at a cytoplasmic Ca(2+ ... The rapid component of exocytosis was not affected by inclusion of millimolar concentrations of the Ca(2+) buffer EGTA but ... In keeping with the idea that secretion is determined by Ca(2+) influx through individual Ca(2+) channels, exocytosis triggered ... Analysis of the kinetics of exocytosis during voltage-clamp depolarizations revealed an early component that reached a peak ...https://www.rdm.ox.ac.uk/publications/9662
Ana Maria Cardenas Diaz
The exocytosis is a key mechanism, crucial for physiological functions such as neurotransmission and hormone secretion. Upon ... Thus the existence of these two modes of exocytosis allows the cells to adjust the release of the active vesicular components ... These two modes of exocytosis are known as "full fusion" and "kiss-and-run" respectively. Why do endocrine cells require ...http://www.pewtrusts.org/en/projects/pew-latin-american-fellows/directory-of-latin-fellows/1992/ana-maria-cardenas-diaz
Suppression of sulfonylurea- and glucose-induced insulin secretion in vitro and in vivo in mice lacking the chloride transport...
This effect was mirrored by a approximately 80% reduction in depolarization-evoked beta cell exocytosis (monitored as increases ... Exocytosis in Type 2 Diabetes - Functional and Genetic Studies of Hormone Secretion ...https://www.ludc.lu.se/erik-renstrom/publication/930a3545-4eb6-423e-8fe4-9d5565e1c038
Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells | JCB
Extent of lysosomal exocytosis is sensitive to cell type and calcium level. To examine if calcium induced exocytosis of ... Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is ... and did not lead to an increase in exocytosis of post-Golgi vesicles. The absence of an effect of calcium on exocytosis of ... Calcium-induced exocytosis is important in the repair of membrane rupture and the invasion of cells by some pathogens (Caler et ...http://jcb.rupress.org/content/159/4/625.long
Ba2+ ions evoke two kinetically distinct patterns of exocytosis in chromaffin cells, but not in neurohypophysial nerve...
"Stimulus- decoupled" exocytosis is slow (approximately 25-40 fF/sec) compared with Ca(2+)-evoked stimulus-coupled exocytosis ( ... Slow exocytosis is initiated by inclusion of Ba2+ ions in the recording pipette and continues steadily for 5-12 min, producing ... Stimulus- coupled exocytosis apparently requires elevated submembrane cation concentrations that dissipate rapidly on ... Intracellular dialysis of NHP terminals with Ba2+ does not evoke exocytosis, nor does it interfere with depolarization-evoked ...http://www.jneurosci.org/content/16/4/1370
The cell. 5. Vesicular traffic. Exocytosis. Atlas of plant and animal histology.
There are two types of exocytosis: constitutive and regulated. Constitutive exocytosis is present in almost every cell and ... Exocytosis. Endocytosis. Endosomes. Lysosomes. In plant cells Vacuoles 6. Non vesicular Peroxisomes. Mitochondria Plastids. ... constitutive and regulated exocytosis. Constitutive exocytosis is present in every cell, it is the default pathway. Molecules ... whereas the other is known as regulated exocytosis, which is present in secretory cells. Regulated exocytosis need a signal for ...http://mmegias.webs.uvigo.es/02-english/5-celulas/5-exocitosis.php
Reductive chain separation of botulinum A toxin - a prerequisite to its inhibitory action on exocytosis in chromaffin cells
The actions ofboth neurotoxins on exocytosis were presently compared using streptolysin O-permeabilized bovine adrenal ... a prerequisite to its inhibitory action on exocytosis in chromaffin cells. In: FEBS Letters, Vol. 248: S. 23-27 ... that the interchain S-S bond of botulinum A toxin must also be split before the neurotoxin can exert its effect on exocytosis. ...https://epub.ub.uni-muenchen.de/7142/
"Role Of Secretory Granule Heterogeneity In Calcium-Triggered Exocytosis " by Tejeshwar Rao
In chromaffin cells, the trigger for stimulus-evoked exocytosis is a rise in intracellular Ca2+. The level of intracellular ... In chromaffin cells, the trigger for stimulus-evoked exocytosis is a rise in intracellular Ca2+. The level of intracellular ... Rao, Tejeshwar, "Role Of Secretory Granule Heterogeneity In Calcium-Triggered Exocytosis" (2016). Wayne State University ... mode of exocytosis). Also, lumenal and membrane proteins are released more slowly from Syt-7 than from Syt-1 granules. The ...http://digitalcommons.wayne.edu/oa_dissertations/1579/
The Toxoplasma Blog: May 2010
Abstract Regulated exocytosis allows the timely delivery of proteins and other macromolecules precisely when they are needed to ... Calcium-regulated exocytosis is a ubiquitous process in eukaryotes, whereby secretory vesicles fuse with the plasma membrane ... Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma. Lourido S, Shuman J, Zhang C, Shokat ... Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma ...http://toxoplasmaparasite.blogspot.com/2010_05_01_archive.html
Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis | JCB
TIRFM analysis reveals exocytosis of post-Golgi vesicles from both daughter cells at the cleavage furrow during cytokinesis. (A ... Exocytosis from both daughter cells occurs at the cleavage furrow during cytokinesis. Arguably, vesicles that accumulate in the ... Visualizing exocytosis at the cleavage furrow by TIRFM might seem unlikely, as this region is generally suspended between ... 4, A, D, and E). Video 6 shows that TIRFM analysis reveals exocytosis of post-Golgi vesicles from both daughter cells at the ...http://jcb.rupress.org/content/181/7/1047
Adenosine Triphosphate and the Late Steps in Calcium-dependent Exocytosis at a Ribbon Synapse | JGP
The Maximum Rate of Exocytosis. Previously, we reported that the maximum rate of exocytosis saturated at calcium concentrations ... 1996) Ultrafast exocytosis elicited by calcium current in synaptic terminals of retinal bipolar neurons. Neuron 17:1241-1249, ... 1996) N-ethylmaleimide-sensitive factor acts at a prefusion step in Ca2+-activated exocytosis. J Biol Chem 271:20223-20226, ... 1996) Exocytosis: proteins and perturbations. Annu Rev Pharmacol Toxicol 36:659-701, pmid:8725405.. ...http://jgp.rupress.org/content/111/2/225
Exocytosis - Wikipedia
"Exocytosis". Oxford Dictionaries. Oxford University Press. Retrieved 2016-01-21.. *^ "Exocytosis". Merriam-Webster Dictionary. ... Look up exocytosis in Wiktionary, the free dictionary.. *Exocytosis at the US National Library of Medicine Medical Subject ... Vesicular exocytosis in prokaryote gram negative bacteria is a third mechanism and latest finding in exocytosis. The periplasm ... Exocytosis in neuronal chemical synapses is Ca2+ triggered and serves interneuronal signalling. Constitutive exocytosis is ...https://en.m.wikipedia.org/wiki/Cellular_secretion
exocytosis - definition and meaning
exocytosis: A process of cellular secretion or excretion in which substances contained in vesicles are discharged from the cell ... The Golgi bud fuses with the inner surface of the axon synaptic membrane, and then, in a process known as exocytosis, bursts ... Regulated exocytosis is essential for many biological processes and many components of the protein trafficking machinery are ... Drag to Playlist exocytosis: vesicular transport eukaryotic cells secrete molecules to the outside of the cell as well as ...https://www.wordnik.com/words/exocytosis