Mutations in the DDR2 kinase gene identify a novel therapeutic target in squamous cell lung cancer. Cancer Discov 2011;1:78-89 ... S2). Given recent reports that EPHA7 is a tumor suppressor (51), we also evaluated whether knockdown of EPHA7 might drive ... Generation and analysis of dasatinib-resistant cell lines. We cultured 2 cell lines, HCC-366 and NCI-H2286, previously shown to ... that ERK1/2 is the most critical effector of the MAPK pathway in the setting of resistance to vemurafenib in melanoma cell ...http://mct.aacrjournals.org/content/13/2/475
Yes-associated protein (YAP) transcriptional coactivator functions in balancing growth and differentiation in skin | PNAS
alpha}-Catenin Is a Tumor Suppressor That Controls Cell Accumulation by Regulating the Localization and Activity of the ... E) Precocious induction of late-differentiation genes by Yap KD in MKs cultured in low-Ca2+ medium. mRNAs were isolated from ... Yorkie is the ultimate effector of the evolutionarily conserved Hippo tumor suppressor pathway (6). Upon Hippo signaling in ... as well as the negative YAP regulator large tumor suppressor (LATS) kinase (3-6). ...http://www.pnas.org/content/108/6/2270
2003) The product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1. Mol Cell 12:841-849. ... To elevate cAMP levels in cultured SCs, forskolin (5 μm; Sigma) was added into serum-free cell culture medium for 30 min. The ... Rac1 knock-out abrogated phosphorylation of the effector p21-activated kinase and decreased NF2/merlin phosphorylation. ... 2008) Tumor suppressor schwannomin/merlin is critical for the organization of Schwann cell contacts in peripheral nerves. J ...http://www.jneurosci.org/content/32/48/17251
Nutrients | Free Full-Text | Selenium in Bone Health: Roles in Antioxidant Protection and Cell Proliferation | HTML
Such findings may relate to roles of Se in antioxidant protection, enhanced immune surveillance and modulation of cell ... In that model, Se treatment increased the expression of humoral defense and tumor suppressor-related genes while decreasing the ... Jurkat and HL60 cells) were cultured in a Se-deficient, serum-free medium, GPX and TRR activities were low and cells did not ... Cell. Biol. 2001, 21, 2755-2766. [Google Scholar]. *Gille, H.; Downward, J. Multiple ras effector pathways contribute to G(1) ...http://www.mdpi.com/2072-6643/5/1/97/htm
Hyperactivation of P21ras and the Hematopoietic-Specific Rho Gtpase, Rac2, Cooperate to Alter the Proliferation of...
Genetic and biochemical evidence that haploinsufficiency of the Nf1 tumor suppressor gene modulates melanocyte and mast cell ... determined by precipitating the active GTPase with a GST fusion of the p21ras binding domain of Raf-1 kinase in an effector ... cultured from Nf1+/− mice demonstrate increased proliferation relative to wild-type cells in response to stem cell factor (SCF ... Mutations in the NF1 tumor suppressor gene cause neurofibromatosis type I (NF1), a disease characterized by the formation of ...http://jem.rupress.org/content/194/1/57
Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human...
... an insertional activation of the mevalonate kinase gene by hepatitis B virus DNA has been reported in the PLC/PRF/5 cell line ( ... Disruption of STAT3 signaling leads to tumor cell invasion through alterations of homotypic cell-cell adhesion complexes. ... B, confluent monolayers of control and silenced (Si1 and Si2) HCT8/S11 cells were wounded and then cultured for 24 h at 37°C. ... 4D). We found consistent activation of Akt and p70/p85 S6 kinase (S6K), two downstream effectors of PI3K, and concomitant ...http://cancerres.aacrjournals.org/content/70/19/7710
Drosophila Apc2 Is a Cytoskeletally-Associated Protein That Regulates Wingless Signaling in the Embryonic Epidermis
... tumor suppressor protein localizes to plasma membrane sites involved in active cell migration. J. Cell Biol. 1996;134:165-180. ... together with previous studies of hAPC in cultured cells, raises the possibility that dAPC2 acts as an effector molecule ... Hayashi S., Rubinfeld B., Souza B., Polakis P., Wieschaus E., Levine A. A Drosophila homolog of the tumor suppressor gene ... the Drosophila homolog of glycogen synthase kinase-3, to regulate engrailed and establish cell fate. Cell. 1992;71:1167-1179. [ ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2156123/?lang=en-ca
2004) RASSF4/AD037 is a potential ras effector/tumor suppressor of the RASSF family. Cancer Res 64:8688-8693. ... cells (see Fig. 1D) and the Atoh1BAC-GFP+ to GFP− cells (see Fig. 2B) using Ppib as the endogenous control gene. Graphs were ... appears to inhibit early endocytosis and recycling in cultured cells (Zuk and Elferink, 2000). An attractive hypothesis is that ... upregulates the cyclin-dependent kinase inhibitor, p21 (Laub et al., 2005). Interestingly, in Merkel cell carcinomas in which ...http://www.jneurosci.org/content/31/30/10859
The Drosophila melanogaster gene brain tumor negatively regulates cell growth and ribosomal RNA synthesis | Development
Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway. Genes ... In contrast to the cell growth regulation pathway of the insulin receptor and its effectors, our results indicate that brat ... Role in hypertrophic growth of cultured cardiomyocytes. J. Biol. Chem. 266, 22003-22009. ... We now indicate that the Drosophila melanogaster tumor suppressor brain tumor (brat) is an inhibitor of cell growth and is a ...http://dev.biologists.org/content/129/2/399
... kinase 1 (DAPK1), 14-3-3 sigma, and the candidate tumor suppressor gene RASSF1A (3). However, comparatively little is known ... All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS) and 1% ... In the murine lung, GATA6 drives the expression of the thyroid transcription factor TTF-1, which is an upstream effector ... Cell culture and cell lines. The A549 and Phoenix amphotrophic cell lines were obtained from the American Type Culture ...http://mcr.aacrjournals.org/content/11/2/161
Drosophila Apc2 Is a Cytoskeletally-Associated Protein That Regulates Wingless Signaling in the Embryonic Epidermis | JCB
... tumor suppressor protein localizes to plasma membrane sites involved in active cell migration. J. Cell Biol. 134:165-180, pmid: ... together with previous studies of hAPC in cultured cells, raises the possibility that dAPC2 acts as an effector molecule ... 1997) A Drosophila homolog of the tumor suppressor gene adenomatous polyposis coli down-regulates β-catenin but its zygotic ... along with Axin and the kinase Zeste-white 3 (Zw3)/glycogen synthase kinase 3β (GSK). In cells of a colon polyp, loss of APC ...http://jcb.rupress.org/content/146/6/1303
Inhibition of Vascular Smooth Muscle Cell Proliferation, Migration, and Survival by the Tumor Suppressor Protein PTEN |...
The effectors of PI 3-kinase are activated by the phospholipid products of PI 3-kinase. In this report, we investigated the ... Schwartzbauer G, Robbins J. The tumour suppressor gene PTEN can regulate cardiac hypertrophy and survival. J Biol Chem. 2001; ... Production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation ... in tumor cells the effects of PI 3-kinase on cell proliferation and survival have been the subject of intense investigation.20 ...http://atvb.ahajournals.org/content/22/5/745
A peptide antigen derived from EGFR T790M is immunogenic in non‑small cell lung cancer
Target cells were labeled with calcein-AM and co-cultured with the effector cells for 4-6 h. The T790M-A-specific CTL line ... Moreover, point mutations within the ABL kinase domain of the BCR-ABL gene are the most common causes of resistance to imatinib ... Rubio V, Stuge TB, Singh N, et al: Ex vivo identification, isolation and analysis of tumor-cytolytic T cells. Nat Med. 9:1377- ... 105 cells/well, target 1×105 cells/well). Right: H-1975 and H1975-A2 cells were used as the targets (effector, 5×104 cells/well ...https://www.spandidos-publications.com/10.3892/ijo.2014.2787
PTEN Induces G1 Cell Cycle Arrest and Decreases Cyclin D3 Levels in Endometrial Carcinoma Cells | Cancer Research
Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway. Genes ... AN3CA, RL-95, and 293T cells were cultured in DMEM (Life Technologies, Inc., Bethesda, MD), and Hec1A cells were cultured in ... Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Mol. Cell. Biol. ... The PTEN/MMAC1/TEP tumor suppressor gene decreases cell growth and induces apoptosis and anoikis in breast cancer cells. ...http://cancerres.aacrjournals.org/content/61/11/4569
Simvastatin inhibits the expression of stemness‑related genes and the metastatic invasion of human cancer cells via destruction...
... we cultured cells in the presence of 5 μM cytochalasin, a well known inhibitor of actin polymerization. Notably, after 6 h of ... where it can bind to a set of effector molecules that are important for maintaining the undifferentiated status of the cell. On ... Sox2 maintains self renewal of tumor-initiating cells in osteosarcomas. Oncogene. 31:2270-2282. 2012. View Article : Google ... regulatory signals that activate kinase cascades or transcriptional factors controlling the activation of proliferative genes ...https://www.spandidos-publications.com/ijo/51/6/1851
Canonical and alternative transcript expression of PAX6 and CXCR4 in pancreatic cancer
... to indicate cell nuclei. The sample shown had a ductal lesion with desmoplastic fibroblasts around the tumor cells. (B) A ... interacts with the promoter of the receptor tyrosine kinase gene MET and promotes gene expression (17,18). While PAX6 is ... and all the cell lines were cultured as previously described (14,18). Cells were transfected using Effectene Transfection ... downstream effector genes of PAX6, other than MET (18), have not been reported. ...https://spandidos-publications.com/ol/13/6/4027
ARF TUMOR-SUPPRESSOR; GENE-EXPRESSION; LOCUS; MUTATIONS; INK4A/ARF; MELANOMA; GENOME; SENESCENCE; NICHE; CELLS National ... In addition, the method presents approaches to target the expression of effector genes, such as regulators of cell fate or cell ... We analyzed the endothelin signaling system in chicken retina and cultured primary chicken Muller cells as well as the human ... Retinal injury induces Müller cell dedifferentiation by activating extracellular signal-regulated kinase (ERK) signaling. ...http://uu.diva-portal.org/smash/person.jsf?pid=authority-person%3A11188
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays |...
Among the genes underrepresented in the tumor cell population, there were a number of potential tumor suppressor genes such as ... For example, genes indicating cell cycle aberrations (cyclin D2, cyclin B1, activator of S-phase kinase, and the cell cycle ... the different source of RNA used because cultured cells may have adapted to in vitro growth conditions and thus express genes ... Although both RalA and RalB are downstream effectors of ras signaling pathway (55) , a distinct function for RalA and RalB has ...http://clincancerres.aacrjournals.org/content/10/15/4944
c-Myc Regulates RNA Splicing of the A-Raf Kinase and Its Activation of the ERK Pathway | Cancer Research
Tumor and Stem Cell Biology c-Myc Regulates RNA Splicing of the A-Raf Kinase and Its Activation of the ERK Pathway. Jens Rauch ... Cell lines. HeLa, GHD-1, HCT116, and NIH3T3 cells were cultured in standard Dulbecco's modified Eagle's medium containing 10% ... Global mapping of c-Myc binding sites and target gene networks in human B cells. Proc Natl Acad Sci U S A 2006;103:17834-9. ... To prove that A-Rafshort is acting directly on Ras and not on downstream effectors, NIH3T3 cells were cotransfected with A-Raf ...http://cancerres.aacrjournals.org/content/71/13/4664
MDMX: from bench to bedside | Journal of Cell Science
Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics. Genes Dev. 18, 1413-1422. ... MDMX regulates cell death and cell cycle exit in cultured retinoblastoma cells in a p53-dependent manner. Moreover, ectopic ... Chen, L., Li, C., Pan, Y. and Chen, J. (2005b). Regulation of p53-MDMX interaction by casein kinase 1 alpha. Mol. Cell. Biol. ... Tumors retaining wild-type p53 are thought to have defects either in effector target genes or in the expression of p53 ...http://jcs.biologists.org/content/120/3/371
Raveh T., Kimchi A. DAP kinase-a proapoptotic gene that functions as a tumor suppressor. Exp. Cell Res., 264: 185-192, 2001. ... Target cells were added to the wells at a concentration of 1 × 104 cells/ml. Effector cells were added to the wells at E:T ... Cells were cultured in 75 cm2 tissue flask (Corning Costar Corp., Cambridge, MA) at 37°C in a fully humidified atmosphere of 5 ... Tumor Cells.. B16F10 melanoma cells with a high metastatic potential and L1210 leukemia cells were used throughout experiments ...http://clincancerres.aacrjournals.org/content/9/8/3124
Suppression of Deacetylase SIRT1 Mediates Tumor-Suppressive NOTCH Response and Offers a Novel Treatment Option in Metastatic...
Tumor suppressor HIC1 directly regulates SIRT1 to modulate p53-dependent DNA-damage responses. Cell 2005;123:437-48. ... Briefly, Ewing sarcoma cells (2 × 104 cells per well) were cultured in 96-well plates at 37°C and exposed to varying ... VH64 and TC252 cells express wild-type TP53, STA-ET-7.3 expresses mutant, SK-N-MC truncated, and A673 no TP53 due to gene ... Ca2+/calmodulin-dependent protein kinase kinase beta phosphorylation of Sirtuin 1 in endothelium is atheroprotective. Proc Natl ...http://cancerres.aacrjournals.org/content/74/22/6578
CASP8 | Cancer Genetics Web
In addition, A549 cells were injected into nude mice, and the effects of shRNA targeting the survivin gene on tumor growth were ... J Cell Physiol. 2015; 230(9):2281-98 [PubMed] Related Publications We determined whether the multi-kinase inhibitor sorafenib ... has shown modest effectiveness and did not follow the outcomes in cultured cells or animal models. Moreover, the molecular ... The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD ...http://www.cancerindex.org/geneweb/CASP8.htm
Laboratory for signal transduction mechanisms of TGF-ß | LUMC
It is well established that TGF-β switches its function from being a tumor suppressor to a tumor promoter during the course of ... Quiescent and activated HSCs were identified as major BMP-9 producing liver cell type. BMP-9 stimulation of cultured ... via serine/threonine kinase receptors and SMAD transcriptional effectors. However, how this pathway is (mis)regulated in cancer ... Moreover, we found that several components in the WNT pathway were enriched among the late TGF-β-target genes, including the ...https://www.lumc.nl/org/moleculaire-celbiologie/research/Aging-and-Signal-transduction/Laboratory-for-signal-transduction-mechanisms-of-TGF-b/
Cell growth assay. Cell lines expressing or not expressing the RhoC gene were cultured in the RPMI 1650 medium. At each time ... PTEN up-regulates the tumor metastasis suppressor gene Drg-1 in prostate and breast cancer. Cancer Res 2004; 64: 7655-60. ... we found that RhoC promotes tumor metastasis but not tumor growth by sequential activation of Pyk2, focal adhesion kinase (FAK ... 5A). Pyk2 is capable of transducing signals via several known pathways, and one of the effectors is FAK which has been shown to ...http://cancerres.aacrjournals.org/content/68/18/7613