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*  The M230L Nonnucleoside Reverse Transcriptase Inhibitor Resistance Mutation in HIV-1 Reverse Transcriptase Impairs Enzymatic...
... demonstrate reductions in RNase H activity and DNA synthesis from tRNA(Lys, 3) that correlate with reductions in replication ... Mutational analysis of primer grip residues has shown their influence on various RT functions, including dNTP binding (46), ... are versatile DNA polymerases endowed with several properties essential for viral replication, i.e., RNA- and DNA-dependent DNA ... RT that contained M230L was also deficient in regard to each of minus-strand DNA synthesis, both DNA- and RNA-dependent ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2876396/
*  Karla Kirkegaard | Stanford Medicine Profiles
... mutational analyses in cells, in vitro activity analyses and RNA substrate modeling studies. The results of these studies ... Protein primers are used to initiate genomic synthesis of several RNA and DNA viruses, although the structural details of the ... Protein priming of viral RNA synthesis plays an essential role in the replication of picornavirus RNA. Both poliovirus and ... Mutational analysis of the polymerase identified four surface residues on the three-dimensional structure of poliovirus ...
  https://med.stanford.edu/profiles/karla-kirkegaard
*  Oncogenomics - Wikipedia
Such excess DNA damage can increase mutational errors during DNA replication due to error-prone translesion synthesis. Excess ... Nucleotide sequence context influences mutation probability and analysis of mutational (mutable) DNA motifs can be essential ... Un-repaired DNA damage is a major cause of mutations that drive carcinogenesis. If DNA repair is deficient, DNA damage tends to ... Such motifs represent the fingerprints of interactions between DNA and mutagens, between DNA and repair/replication/ ...
  https://en.wikipedia.org/wiki/Oncogenomics
*  BRCA1 - Wikipedia
Such excess DNA damage may increase mutational errors during DNA replication due to error-prone translesion synthesis. Excess ... "Mutational analysis of BRCA1 and BRCA2 and clinicopathologic analysis of ovarian cancer in 82 ovarian cancer families: two ... BRCA1 directly binds to DNA, with higher affinity for branched DNA structures. This ability to bind to DNA contributes to its ... and deficiencies in DNA repair appears to underlie many forms of cancer. If DNA repair is deficient, DNA damage tends to ...
  https://en.wikipedia.org/wiki/BRCA1
*  Chargaff kuralları - Vikipedi
Rudner R, Karkas JD, Chargaff E (1968). Separation of B. subtilis DNA into complementary strands. III. Direct Analysis. Proc ... species-specific and DNA strand-specific mutational biases". Nucleic Acids Res 27 (7): 1642-1649. DOI:10.1093/nar/27.7.1642. ... "Pyrimidine clusters on the transcribing strands of DNA and their possible role in the initiation of RNA synthesis". Cold Spring ... McLean MJ, Wolfe KH, Devine KM (1998). "Base composition skews, replication orientation, and gene orientation in 12 prokaryote ...
  https://tr.wikipedia.org/wiki/Chargaff_kurallar%C4%B1
*  Inactivation of the Escherichia coli priA DNA replication protein induces the SOS response.
Mutational analysis of primosome assembly sites. I. Distinct classes of mutants in the pBR322 Escherichia coli factor Y DNA ... Stuitje AR, Weisbeek PJ, Meijer M. Initiation signals for complementary strand DNA synthesis in the region of the replication ... Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication ... replication factor Y, required for in vitro synthesis of phiX174 DNA. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3342-3346. [ ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC209016/?lang=en-ca
*  Chromosome segregation control by Escherichia coli ObgE GTPase - Foti - 2007 - Molecular Microbiology - Wiley Online Library
Jonathan Berthon, Ryosuke Fujikane, Patrick Forterre, When DNA replication and protein synthesis come together, Trends in ... A mutational analysis of the C. crescentus orthologue suggests accumulation of 50S ribosomal subunits at the expense of 70S ... Flow cytometry analysis for DNA content, cell size and vital staining. For DNA content and cell size, ethanol fixed cells ... Despite this problem in chromosome partitioning, DNA replication continued after ObgE depletion. DNA content in these ObgE- ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2007.05811.x/full
*  Differential effects of cisplatin and MNNG on dna mutants of Escherichia coli
Impairment of lagging strand synthesis triggers the formation of a RuvABC substrate at replication forks. EMBO J. 2001;20:619- ... Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity. J Bacteriol. 1997;179:7435-7445. ... Vandewiele D, Fernandez de Henestrosa AR, Timms AR, Bridges BA, Woodgate R. Sequence analysis and phenotypes of five ... We have examined various DNA replication mutants and we describe two instances where dna gene products alter the cellular ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2927670/
*  APOBEC1 - Wikipedia
The antiviral properties of A1 extend to both DNA and RNA due to its deamination function, which can hinder DNA replication and ... Teng BB, Ochsner S, Zhang Q, Soman KV, Lau PP, Chan L (1999). "Mutational analysis of apolipoprotein B mRNA editing enzyme ( ... While A1 has a negligible impact on human lipid synthesis, at high concentrations it can be genotoxic. Its diffusion toward the ... In single growth assays, A1 has been found to impact HIV replications. Additionally, A1 has reduced Hepatitus B virus (HBV) DNA ...
  https://en.wikipedia.org/wiki/APOBEC1
*  Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase ι | The EMBO Journal
Kinetic analysis of replication products. Time course reactions using the standard replication conditions were initially ... Replication fork bypass of a pyrimidine dimer blocking leading strand DNA synthesis. J Biol Chem, 272, 13945-13954. ... Analysis of the mutation spectra generated from UV‐irradiated cultured human cell lines reveals that mutational events are ... Yuan F, Zhang Y, Rajpal DK, Wu X, Guo D, Wang M, Taylor JS and Wang Z (2000) Specificity of DNA lesion bypass by the yeast DNA ...
  http://emboj.embopress.org/content/19/19/5259
*  RAD52 - Wikipedia
Such excess DNA damage may increase mutational errors during DNA replication due to error-prone translesion synthesis. Excess ... "Analysis of the human replication protein A:Rad52 complex: evidence for crosstalk between RPA32, RPA70, Rad52 and DNA". Journal ... This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing ... Bernstein C, Prasad AR, Nfonsam V, Bernstein H. (2013). DNA Damage, DNA Repair and Cancer, New Research Directions in DNA ...
  https://en.wikipedia.org/wiki/RAD52
*  Biochemistry of Viral Replication - Maurice Green
Green, M; Ishino, M; Loewenstein, P M (1989) Mutational analysis of HIV-1 Tat minimal domain peptides: identification of trans- ... induction of cellular DNA synthesis, cell immortalization, cell transformation as well as, paradoxically, the inhibition of ... autonomous protein domains for induction of cellular DNA synthesis and for trans activation. J Virol 64:6121-9 ... Biochemistry of Viral Replication Green, Maurice Saint Louis University, Saint Louis, MO, United States ...
  http://grantome.com/grant/NIH/K06-AI004739-53
*  Genetic and Molecular Analysis of Yeast DNA Replication - Robert Sclafani
The long-term objective of this proposal is to understand the cellular processes that regulate high-fidelity eukaryotic DNA ... DDK may also be important for replication restart after DNA damage and TLS. A combination of mutational analysis, whole-genome ... trans-lesion synthesis). Our studies have shown that DDK regulates both spontaneous and induced mutagenesis by DNA polymerase ... Genetic and Molecular Analysis of Yeast DNA Replication Sclafani, Robert A. University of Colorado Denver, Aurora, CO, United ...
  http://grantome.com/grant/NIH/R01-GM035078-22
*  Best Practices for Mapping Replication Origins in Eukaryotic Chromosomes - Current Protocols
Understanding the regulatory principles ensuring complete DNA replication in each cell division is critical for deciphering the ... Discrete start sites for DNA synthesis in the yeast ARS1 origin. Science 279:95‐98. ... High‐resolution analysis of four efficient yeast replication origins reveals new insights into the ORC and putative MCM binding ... Replication‐associated mutational asymmetry in the human genome. Mol. Biol. Evol. 28:2327‐2337. ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb2218.html
*  Beverly S. Mitchell, M.D. | Stanford Medicine Profiles
... lead to the synthesis of guanine nucleotides required for cellular entry into S phase and the initiation of DNA replication. ... tandem CRE and POS sites are of major functional importance as judged by mutational and electrophoretic mobility shift analyses ... On an M13 DNA template, RNA-primed DNA synthesis catalyzed by the purified complex of DNA primase (EC 2.7.7.6) and DNA ... and inhibition of DNA synthesis. To test the hypothesis that dATP and dGTP accumulation inhibit DNA synthesis by inhibiting the ...
  https://med.stanford.edu/profiles/beverly-mitchell
*  Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression system. -...
Mutational analysis of the promoter required for influenza virus virion RNA synthesis. ... Influenza virus NS 1 protein interacts with viral transcription - replication complexes in vivo. *Rosa, Ma, Mário, Thomas Zu, ... Molecular cloning of natural paramyxovirus copy-back defective interfering RNAs and their expression from DNA. ... polymerase subunit . domains in the influenza A virus PB 2 Mutational analysis identifies functional. *Beatriz Perales, SUSANA ...
  https://www.semanticscholar.org/paper/Synthesis-of-biologically-active-influenza-virus-a-Mena-Luna/8bd946b7482abd497404d1e9bad537b96ecfd51e
*  Metabolism, Genomics, and DNA Repair in the Mouse Aging Liver
... this adduct in genomic DNA may lead to a point mutation upon DNA replication. During DNA replication, 8-oxo-deoxyguanines ... Podlutsky, I. I. Dianova, V. N. Podust, V. A. Bohr, and G. L. Dianov, "Human DNA polymerase β initiates DNA synthesis during ... A. Collins, C. Gedik, N. Vaughan et al., "Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by ... M. E. T. Dolle, W. K. Snyder, D. B. Dunson, and J. Vijg, "Mutational fingerprints of aging," Nucleic Acids Research, vol. 30, ...
  https://www.hindawi.com/journals/cggr/2011/859415/
*  Aurora Kinases as Anticancer Drug Targets | Clinical Cancer Research
This selective killing may be achieved either by interfering with the synthesis/replication of DNA (e.g., using agents such as ... Analysis of phosphorylated histone H3 Ser10 levels can show the effects of both aurora A and aurora B inhibitors (Fig. 4). ... The tumor cell phenotype is the consequence of a relatively high level of genetic instability, with sequential mutational and ... DNA methylation promotes aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains. J Cell Sci 2007;120:101-14. ...
  http://clincancerres.aacrjournals.org/content/14/6/1639.long
*  Φ29 DNA polymerase - Wikipedia
"Highly efficient DNA synthesis by the phage Φ29 DNA polymerase. Symmetrical mode of DNA replication". J. Biol. Chem. 264 (15): ... de Vega M, Lázaro JM, Salas M, Blanco L (1998). "Mutational analysis of phi29 DNA polymerase residues acting as ssDNA ligands ... motif of phi29 DNA polymerase as a metal ligand during both TP-primed and DNA-primed DNA synthesis". J Mol Biol. 283 (3): 633- ... of phi29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication". ...
  https://en.wikipedia.org/wiki/%CE%A629_DNA_polymerase
*  Microviridae - Wikipedia
Synthesis of bacteriophage phi X174 in vitro: mechanism of switch from DNA replication to DNA packaging" Cell 47(1) 99-106 ... Mutational analysis of the bacteriophage phi X174 replication origin" J Mol Biol 198(1) 51-61 Fluit AC, Baas PD, Jansz HS (1985 ... Replication of the genome now begins via a rolling circle mechanism. The host's DNA polymerase converts the single-stranded DNA ... Protein A* inhibits host DNA replication. Unlike protein A it is capable of cleaving the phi X viral DNA in the presence of ...
  https://en.wikipedia.org/wiki/Microviridae
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
This method is based on replication of a ColE1 plasmid encoding the desired sequence by a low-fidelity variant of DNA ... The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, ... Mutational load can be thought of as a balance between selection for or against a deleterious mutation and its production by de ... Analysis of H-bonding residues of blaA variants with amoxicillin and clavulanic acid revealed that if amino acid residues of a ...
  https://www.jove.com/visualize/abstract/25919756/structural-variabilities-lactamase-blaa-different-biovars-yersinia
*  Sandwalk: Stephen Meyer Talks About Junk DNA
... even if they do not direct protein synthesis. ID theorists do not deny that mutational processes might have degraded or "broken ... regulate DNA replication, (2) regulate transcription, (3) mark sites for programmed rearrangements of genetic material, (4) ... But there's a problem with that analysis, and it's a serious one. The problem is the genome itself: it's not perfect. In fact, ... Here's what Meyer says about junk DNA. Consider the case of so-called junk DNA-the DNA that does not code for proteins found in ...
  http://sandwalk.blogspot.com/2011/10/stephen-meyer-talks-about-junk-dna.html
*  PLOS Genetics: PCNA Ubiquitination Is Important, But Not Essential for Translesion DNA Synthesis in Mammalian Cells
... which help replication to bypass the lesions. A major universal tolerance mechanism is translesion DNA synthesis (TLS), in ... but there exist secondary yet significant TLS mechanisms that operate in its absence and have an altered mutational outcome. ... a sliding DNA clamp that tethers the DNA polymerases to DNA, which functions to recruit the TLS DNA polymerase to the damaged ... In cases when the removal of DNA damage and restoration of the original sequence prior to replication is impossible, cells ...
  http://journals.plos.org/plosgenetics/article/authors?id=10.1371/journal.pgen.1002262&imageURI=info:doi/10.1371/journal.pgen.1002262.g006
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
This method is based on replication of a ColE1 plasmid encoding the desired sequence by a low-fidelity variant of DNA ... The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, ... Structure-function analyses of the BRCA1 have been complicated by the lack of a robust assay for the full length BRCA1 protein ... Mutational load can be thought of as a balance between selection for or against a deleterious mutation and its production by de ...
  https://www.jove.com/visualize/abstract/22132097/a-novel-mutation-hsd17b10-gene-10-year-old-boy-with-refractory
*  Squaring up to DNA: pentapeptide repeat proteins and DNA mimicry | SpringerLink
Guo Q, Weng J, Xu X, Wang M, Wang X, Ye X, Wang W, Wang M (2010) A mutational analysis and molecular dynamics simulation of ... Zechiedrich EL, Cozzarelli NR (1995) Roles of topoisomerase IV and DNA gyrase in DNA unlinking during replication in ... Growth phase dependence of the activation of a bacterial gene for carotenoid synthesis by blue light. EMBO J 12(4):1265-1275 ... Williams NL, Maxwell A (1999a) Locking the DNA gate of DNA gyrase: investigating the effects on DNA cleavage and ATP hydrolysis ...
  https://link.springer.com/article/10.1007/s00253-014-6151-3