Complementary DNA definition | Drugs.com
Definition of complementary DNA. Provided by Stedman's medical dictionary and Drugs.com. Includes medical terms and definitions ... single-stranded DNA that is complementary to messenger RNA;. *DNA that has been synthesized from mRNA by the action of reverse ...https://www.drugs.com/dict/complementary-dna.html
Complementary DNA definition and meaning | Collins English Dictionary
... a form of DNA artificially synthesized from a messenger RNA template and used in genetic... , Meaning, pronunciation, ... Trends of 'complementary DNA'. Used Rarely. complementary DNA is in the lower 50% of commonly used words in the Collins ... a form of DNA artificially synthesized from a messenger RNA template and used in genetic engineering to produce gene clones ...https://www.collinsdictionary.com/dictionary/english/complementary-dna
Quantitative monitoring of gene expression patterns with a complementary DNA microarray. - PubMed - NCBI
Quantitative monitoring of gene expression patterns with a complementary DNA microarray.. Schena M1, Shalon D, Davis RW, Brown ... Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression ...https://www.ncbi.nlm.nih.gov/pubmed/7569999
Complementary DNA - Wikipedia
In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) ... generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G ... This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. To synthesize an additional DNA strand ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ...https://en.wikipedia.org/wiki/Complementary_DNA
A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large...
A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA ...http://pubs.rsc.org/en/content/articlelanding/2018/ob/c8ob00062j/unauth
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays |...
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays. ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ... For PCR of genomic DNA, DNA was extracted from samples by incubating overnight at 55°C with lysis buffer containing 10 mm Tris- ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ...http://clincancerres.aacrjournals.org/content/10/15/4944
Molecular Cloning and Characterization of the Complementary DNA of an Mr 110,000 Antigen Expressed by Human Gastric Carcinoma...
Molecular Cloning and Characterization of the Complementary DNA of an Mr 110,000 Antigen Expressed by Human Gastric Carcinoma ... Molecular Cloning and Characterization of the Complementary DNA of an Mr 110,000 Antigen Expressed by Human Gastric Carcinoma ... Molecular Cloning and Characterization of the Complementary DNA of an Mr 110,000 Antigen Expressed by Human Gastric Carcinoma ... We describe the molecular cloning and sequence analyses of overlapping clones that constitute a full-length complementary DNA ...http://cancerres.aacrjournals.org/content/54/14/3831?ijkey=90393d8dd21178c341b2e6e7ecba842a4b5ce92e&keytype2=tf_ipsecsha
Complementary DNA cloning and constitutive expression of cytoc...
Complementary DNA cloning and constitutive expression of cytochrome P450 1C1 in the gills of carp (Cyprinus carpio).: ... Complementary DNA cloning and constitutive expression of cytochrome P450 1C1 in the gills of carp (Cyprinus carpio).. Authors * ... A new complementary DNA of the CYP1C subfamily encoding CYP1C1 was isolated from carp liver after intraperitoneal injection of ...https://www.mysciencework.com/publication/show/complementary-dna-cloning-constitutive-expression-cytochrome-p450-1c1-gills-carp-cyprinus-carpio-633ae3e9
Aptamer-Based Electrochemical Sensors with Aptamer−Complementary DNA Oligonucleotides as Probe
DNA Barcoding: Complementary and alternative medicines
In addition they found DNA from pit vipers, frogs and trace amounts of cat and dog DNA. Whether the animal products were ... Of the 26 TCMs investigated, all but two can be classified as non-compliant on the grounds of DNA, toxicology and heavy metals ... The researchers also found one of the herbal medicines for sale had trace amounts of snow leopard DNA in it. ... Metabarcoding is a rapid method of biodiversity assessment that combines two technologies: DNA based identification and high- ...http://dna-barcoding.blogspot.com/2015/12/complementary-and-alternative-medicines.html
Complementary DNA Cloning, Protein Expression, and Characterization of Alpha-Class GSTs from Macaca fascicularis Liver :...
Large species differences exist in sensitivity to aflatoxin B1 (AFB1)-induced liver cancer. Mice are resistant to AFB1-induced liver cancer because they express an alpha-class GST (mGSTA3-3) that has high activity toward the reactive intermediate aflatoxin B1-8,9-epoxide (AFBO). Rats constitutively express only small amounts of a GST with high AFBO activity (rGSTA5-5) and thus are sensitive to AFB1-induced hepatocarcinogenesis, although induction of rGSTA5-5 can confer resistance in rats. In contrast to rodents, constitutively expressed human hepatic alpha-class GSTs have little or no AFBO detoxifying activity. Recently, we found that the nonhuman primate, Macaca fascicularis (Mf), has significant constitutive hepatic GST activity toward AFBO and most of this activity belongs to mu-class GSTs. To determine if any alpha-class GSTs in Mf liver have AFBO activity, a cDNA library from a male Mf liver was constructed and screened using the human alpha-class GstA1 cDNA as a probe. Three different cDNA ...http://oxfordindex.oup.com/view/10.1093/toxsci/70.1.20
Bromine in the structure of Crystal Structure of A 7-Aminoactinomycin D Complex With Non-Complementary Dna (pdb 1unj)
Crystal Structure of A 7-Aminoactinomycin D Complex With Non-Complementary Dna ... Bromine in the structure of Crystal Structure of A 7-Aminoactinomycin D Complex With Non-Complementary Dna (pdb 1unj). ... sites of Bromine atom in the structure of Crystal Structure of A 7-Aminoactinomycin D Complex With Non-Complementary Dna (pdb ...http://bromine.atomistry.com/pdb1unj.html
Detection of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary...
... where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. The ... where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. The ... of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary DNA.. *. ... of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary DNA.}, ...https://www.semanticscholar.org/paper/Detection-of-virus-specific-RNA-in-simian-sarcoma-Kaufman-Gallo/534e6695efc6a5d9ffbc573b17bdf8de94a9ce53
GM-CSF Restores Innate, But Not Adaptive, Immune Responses in Glucocorticoid-Immunosuppressed Human Blood In Vitro | The...
Complementary DNA expression array. Equal numbers of PBMC obtained from different healthy donors in the same setting were ... After 5 days of incubation, the culture medium was removed, the cells were fixed, and the DNA was denatured for 0.5 h. ...http://www.jimmunol.org/content/171/2/938
Patent US7507873 - Transgenic avians containing recombinant ovomucoid promoters - Google Patents
The term "antisense DNA" as used herein refers to a gene sequence DNA that has a nucleotide sequence complementary to the " ... The term "antisense strand" as used herein refers to the single strand DNA molecule of a genomic DNA that is complementary with ... for example that encoded by an antisense DNA or synthesized complementary with the antisense DNA). Antisense RNA is capable of ... A recombinant DNA molecule of the present invention can be transferred into the extra-chromosomal or genomic DNA of the host. ...http://www.google.com/patents/US7507873?dq=5920316
COMPLEMENTARY DNA CLONING. The conversion of mRNA into cDNA for the purposes of functional studies is a complex, interrelated ... eIF-4GII DNA refers to a DNA fragment of eIF-4GII used as a positive control for DNA hybridizations. Oligos used as probes on ... the hydrolysis of RNA from the RNA-DNA hybrid, and the copying of single-stranded DNA into double-stranded DNA. The two most ... E. coli DH10β cells were grown at 30°C, and supercoiled DNA (lane 1) or BamHI-digested DNA (lane 2) was analyzed on a 1% ...http://physiolgenomics.physiology.org/content/6/2/57
Features of Gene Structure, Organization, and Expression That Are Providing Unique Insights into Molecular Evolution and...
Synthesis of double-stranded DNA complementary to lysozyme. ovomucoid, and ovalbumin mRNAs. J. Biot Cheer. 253: 2483-2495. ... Sanger, F., and Coulson, A. R., 1975, A rapid method for determining sequences in DNA by primed synthesis with DNA polymerise, ... Correlation of DNA exonic regions with protein structural units in haemoglobin, Nature 291: 90-92.PubMedCrossRefGoogle Scholar ... DNA sequence of two closely linked human leukocyte interferon genes, Science 212: 1159-1162.PubMedCrossRefGoogle Scholar ...https://link.springer.com/chapter/10.1007/978-1-4684-4283-0_9
The N-Terminal Juxtamembranous Domain of KCNQ1 Is Critical for Channel Surface Expression | Circulation Research
Complementary DNA Constructs and Mutagenesis. Classical polymerase chain reaction based methods, described in the online data ... the transfection mixture contained 200 ng of WT plus 200 ng of mutant-KCNQ1 complementary DNAs (cDNAs). In the latter ...http://circres.ahajournals.org/content/99/10/1076
Patent US20020164819 - Sample preparing arrangement and a method relating to such an arrangement - Google Patents
Another example would be DNA-complementary DNA (or PNA) reaction. The reaction may occur spontaneously over many sites of the ... In particular, this could be used to synthesize DNA strands or (using appropriate well-known techniques) to investigate the ...http://www.google.ca/patents/US20020164819
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme - Cetus Corporation
... any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary ... A method of cleaving double stranded DNA.. EP0138242. 1985-04-24. 435/172.3. Method of preparing DNA complementary to the ... "Activated" DNA is a native preparation of DNA after partial hydrolysis with DNase I until 5% of the DNA was transferred to the ... A process for amplifying at least one specific DNA sequenc contained in a DNA or a mixture of nucleic acids, wherein the DNA ...http://www.freepatentsonline.com/4965188.html
Avenin fails to induce a Th1 response in coeliac tissue following in vitro culture | Gut
RNA extraction and complementary DNA (cDNA) synthesis. Whole biopsies were homogenised and total RNA isolated by the guanidium ... Primers or probes were designed to cross an exon-exon boundary to ensure that genomic DNA was not being amplified. BLAST ...http://gut.bmj.com/content/52/1/47
How Do You Build a Hot Tub Base? | Reference.com
How Do You Determine the Base Sequence of a Complementary DNA Strand?. ...https://www.reference.com/home-garden/build-hot-tub-base-6badec7942c9de4f
How many base codes are there for one amino acid? | Reference.com
How do you determine the base sequence of a complementary DNA strand?. A: Wageningen University explains that DNA bases only ... so the complementary DNA sequence can be read out by replacing each base with ... Full Answer , Filed Under: * Molecular ...https://www.reference.com/science/many-base-codes-one-amino-acid-9b073acfb9baabb0
RNA and Complementary DNA Preparation for T-Cell Repertoire Analysis.. RNA was extracted using guanidium hydrochloride- ... Five C57BL/6 mice in each group were immunized with rAd-hTRP-2, rAd-EGFP, plasmid DNA encoding p15E (p15E-DNA), or rVV ... Plasmids were purified with Qiagen columns assuring an endotoxin-free DNA preparation (Qiagen GmbH, Hilden, Germany). For DNA ... DNA Immunization.. Preparation of the VR1055-P15 plasmid encoding the p15E portion of the env gene has been described ...http://cancerres.aacrjournals.org/content/64/21/8068
Hippo signaling is required for Notch-dependent smooth muscle differentiation of neural crest | Development
RNA isolation, complementary DNA synthesis and quantitative real-time PCR. RNA was harvested from one 100-mm dish of untreated ... Yap and Taz have no known intrinsic DNA-binding capabilities and therefore require interaction with a DNA-binding molecule to ... Complementary DNA (cDNA) was synthesized with the Superscript III system (Invitrogen). Quantitative RT-PCR was performed in ... In canonical mammalian Hippo signaling, the DNA-binding moiety is one of four homologous Tead factors, but Yap and Taz can ...http://dev.biologists.org/content/142/17/2962
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