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*  Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with...
Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with ... The triglyceride-synthesizing enzyme acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) plays a critical role in hepatitis C ...
  https://www.sigmaaldrich.com/catalog/papers/23420847
*  Microbiology Society Journals | Molecular cloning and overexpression of |em class=''|DGA1|/em|, an acyl-CoA-dependent...
Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (DGA1), of the ... diacylglycerol acyltransferase 2 (DGAT2) from other organisms. The cDNA of RfDGA1 was cloned into the yeast expression vector ... The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is ... 16. Chen Z, Liu P, Liu Y, Tang H, Chen Y et al. Identification and characterization of a type-2 diacylglycerol acyltransferase ...
  http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000584
*  DGAT2L6 antibody | acris-antibodies.com
DC3, Diacylglycerol O-acyltransferase 2-like protein 6, Diacylglycerol O-acyltransferase candidate 3 ... Lenti ORF particles, DGAT2L6 (mGFP-tagged) - Human diacylglycerol O-acyltransferase 2-like 6 (DGAT2L6), 200 uL, ,10^7 TU/mL. ... Lenti ORF particles, DGAT2L6 (Myc-DDK tagged) - Human diacylglycerol O-acyltransferase 2-like 6 (DGAT2L6) , 200 uL, ,10^7 TU/mL ... Lenti ORF particles, Dgat2l6 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 2-like 6 (Dgat2l6), 200 uL, ,10^7 TU/mL ...
  https://www.acris-antibodies.com/target/dgat2l6-antibody.htm
*  Birkbeck Institutional Research Online
Diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in the triacylglyceride synthesis pathway. Bovine DGAT1 is an ...
  http://eprints.bbk.ac.uk/11764/
*  Expression of tung tree diacylglycerol acyltransferase 1 in E. coli | BMC Biotechnology | Full Text
Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, Hawkins DJ: DGAT2 is a new diacylglycerol acyltransferase gene family: ... Cheng D, Meegalla RL, He B, Cromley DA, Billheimer JT, Young PR: Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric ... Diacylglycerol acyltransferase families (DGATs) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis ... Hobbs DH, Lu C, Hills MJ: Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its ...
  https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-73
*  Effect of breed and Diacylglycerol acyltransferase 1 gene polymorphism on milk production traits in Beninese White Fulani and...
Diacylglycerol acyltransferase 1 (DGAT1) is a potential candidate gene with a non-conservative substitution of lysine by alanine (K232A) in exon 8 having a major effect on milk production traits in cattle. The aim of this study was to analyze the allele and genotype frequency, and investigate the association of the DGAT1 K232A polymorphism with milk production traits in indigenous White Fulani and Borgou cattle breeds in Benin. In total, 103 White Fulani and 103 Borgou were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms and validated by Sanger sequencing. The genotypic frequencies of KK, KA and AA in White Fulani and Borgou breeds were 0.83, 0.16, 0.01 and 0.57, 0.39, 0.04 respectively. Frequencies of K and A alleles were 0.91 and 0.09, and 0.77 and 0.23 in White Fulani and Borgou breeds respectively. The White Fulani cows showed higher daily milk yield (P, 0.01), lactose content (P,0.001), protein yield (P, 0.01) and fat yield (P, 0.01) ...
  http://globalscienceresearchjournals.org/gjabg/221220173711?view=print
*  The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells | Garvan Institute of Medical Research
Expression of the long noncoding RNA (lncRNA) SPRY4-IT1 is low in normal human melanocytes but high in melanoma cells. siRNA knockdown of SPRY4-IT1 blocks melanoma cell invasion and proliferation, and increases apoptosis. To investigate its function further, we affinity purified SPRY4-IT1 from melanoma cells and used mass spectrometry to identify the protein lipin 2, an enzyme that converts phosphatidate to diacylglycerol (DAG), as a major binding partner. SPRY4-IT1 knockdown increases the accumulation of lipin2 protein and upregulate the expression of diacylglycerol O-acyltransferase 2 (DGAT2) an enzyme involved in the conversion of DAG to triacylglycerol (TAG). When SPRY4-IT1 knockdown and control melanoma cells were subjected to shotgun lipidomics, an MS-based assay that permits the quantification of changes in the cellular lipid profile, we found that SPRY4-IT1 knockdown induced significant changes in a number of lipid species, including increased acyl ...
  https://www.garvan.org.au/research/publications/12621
*  Unitus DSpace
Title: Exploring polymorphisms and effects of candidate genes on milk fat quality in dairy sheep Authors: Crisà, Alessandra; Marchitelli, Cinzia; Pariset, Lorraine; Contarini, Giovanna; Signorelli, Federica; Napolitano, Francesco; Catillo, Gennaro; Valentini, Alessio; Moioli, Bianca Abstract: Aim of the present study was to investigate the genetic control of the fatty acid (FA) composition in milk from three breeds of sheep: Altamurana, Gentile di Puglia and Sarda. SNP within genes, encoding enzymes putatively involved in the synthesis and metabolism of milk fat, were selected for analysis, and the allele substitution effects were determined for 16 genes, that were polymorphic in the three sheep breeds, upon the milk fat composition. Four genes (alpha-1-antichymotrypsin-2; diacylglycerol O-acyltransferase homolog-2; propionyl Coenzyme A carboxylase, beta polypeptide; insulin-like growth factor-I) play a role in the desaturation of stearic FA into polyunsaturated fatty acids ...
  http://dspace.unitus.it/feed/rss_1.0/author?author=Contarini%2C+Giovanna
*  CAZy - CE1
acetyl xylan esterase (EC 3.1.1.72); cinnamoyl esterase (EC 3.1.1.-); feruloyl esterase (EC 3.1.1.73); carboxylesterase (EC 3.1.1.1); S-formylglutathione hydrolase (EC 3.1.2.12); diacylglycerol O-acyltransferase (EC 2.3.1.20); trehalose 6-O-mycolyltransferase (EC 2.3.1.122 ...
  http://www.cazy.org/CE1_bacteria.html?debut_TAXO=300
*  DIACYLGLYCEROL ACYLTRANSFERASE INHIBITORS - Patent application
0272]To a solution of above (2-chloro-benzoylamino)-acetic acid methyl ester (6 g, 26 mmol) in 30 mL of methanol, was added three equivalents of lithium hydroxide hydrate in 10 mL of water. The solution was stirred at room temperature for 1 hour, concentrated and mixed with water. Citric acid was added until pH of the solution was adjusted to pH 2 to 3. The mixture was extracted with ethyl acetate and the organic layer was washed with water and brine, dried over MgSO4, filtered and concentrated to dryness to give (2-chloro-benzoylamino)-acetic acid as a solid. To a solution of (2-chloro-benzoylamino)-acetic acid in 40 mL of acetone at -20° C. was added excess of trifluoroacetic anhydride. The mixture was warmed up to room temperature and stirred overnight. The solvent was removed under vacuum. The residue was poured into 400 mL of water and stirred for 20 min. The solid was filtered out and washed with 2×100 mL of water, and dried under vacuum to give ...
  http://www.patentsencyclopedia.com/app/20100190979
*  A Plant Acyltransferase Involved in Triacylglycerol Biosynthesis Complements an Escherichia Coli sn-1-acylglycerol-3-phosphate...
The second acylation reaction in glycerolipid biosynthesis is catalyzed by an sn-1-acylglycerol-3-phosphate acyltransferase. The enzyme of Limnanthes douglusii involved in triacylglycerol synthesis has an unusual specificity for very long chain acyl groups in both of its substrates, namely acyl-CoA and sn-1-acylglycerol-3-phosphate, and causes the enrichment of erucoyl groups in the sn-2 position of the seed oil of this plant species. We have isolated a cDNA clone encoding this embryo-specific, microsomal acyltransferase via heterologous complementation of an Escherichia coli mutant deficient in sn -1-acyl-glycerol-3-phosphate acyltransferase activity. The open reading frame of the cDNA insert encodes a protein with a length of 281 amino acids, with three predicted membrane-spanning domains and of about 31.7 kDa. The sequence exhibits substantial sequence similaritiy to the sn-1-acylglycerol-3-phosphate acyltransferase of E. coli. The ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1995.806zz.x/abstract
*  Storage Rates of Circulating Free Fatty Acid Into Adipose Tissue During Eating or Walking in Humans | Diabetes
We measured circulating FFA storage rates into UBSQ and LBSQ fat under postprandial and physical activity conditions. Unexpectedly, the absolute rates of palmitate storage per kilogram fat were relatively similar between postabsorptive, postprandial, and walking conditions (Table 5). This was surprising because the postprandial state favors fat storage, whereas the postabsorptive and walking conditions do not. We also found that both sexes preferentially stored FFAs in UBSQ relative to LBSQ fat in the postprandial state, whereas the walking condition favored LBSQ storage in women only. Lastly, in LBSQ adipocytes, CD36 content independently predicted postprandial palmitate storage, whereas DGAT activity independently predicted palmitate storage during walking.. Using arterio-venous balance/isotopic tracer methodology, Bickerton et al. (7) found FFA uptake rates into abdominal subcutaneous fat in men in the early and midpostprandial period of 82-183 nmol ⋅ 100 g fat−1 ⋅ min−1 ...
  http://diabetes.diabetesjournals.org/content/61/2/329?with-ds=yes
*  Pet10p is a yeast perilipin that stabilizes lipid droplets and promotes their assembly | JCB
Pet10p is a yeast lipid droplet protein of unknown function. We show that it binds specifically to and is stabilized by droplets containing triacylglycerol (TG). Droplets isolated from cells with a PET10 deletion strongly aggregate, appear fragile, and fuse in vivo when cells are cultured in oleic acid. Pet10p binds early to nascent droplets, and their rate of appearance is decreased in pet10Δ. Moreover, Pet10p functionally interacts with the endoplasmic reticulum droplet assembly factors seipin and Fit2 to maintain proper droplet morphology. The activity of Dga1p, a diacylglycerol acyltransferase, and TG accumulation were both 30-35% lower in the absence of Pet10p. Pet10p contains a PAT domain, a defining property of perilipins, which was not previously known to exist in yeast. We propose that the core functions of Pet10p and other perilipins extend beyond protection from lipases and include the preservation of droplet integrity as well as collaboration with seipin and Fit2 ...
  http://jcb.rupress.org/content/early/2017/08/10/jcb.201610013
*  The High-fat Hep C Diet: NASH and dietary cholesterol - the potted version
One of the most common forms of liver disease worldwide is steatohepatitis, which encompasses both alcoholic (ASH) and nonalcoholic steatohepatitis (NASH). While the pathogenesis of these diseases are obviously different, they exhibit indistinguisable histological features and commom underlying mechanisms of disease progression that begin with hepatic steatosis. Although fat infiltration in these diseases is heterogenous consisting primarily of triglyceride, free fatty acids and cholesterol, recent studies have pointed to an emerging role for cholesterol, particularly in mitochondria, in the progression from steatosis to steatohepatitis as it sensitizes hepatocytes to inflammatory cytokines (e.g. TNF/Fas ligand) [45]. Moreover, massive triglyceride accumulation in the liver of transgenic mice overexpressing DGAT2 did not impair insulin sensitivity, a hallmark feature of NASH [46]. In addition, unchanged hepatic free fatty acids levels were reported in patients with NASH compared to healthy ...
  http://hopefulgeranium.blogspot.com/2013/07/nash-and-dietary-cholesterol-potted.html
*  CARBOXY OXAZOLE OR THIAZOLE COMPOUNDS AS DGAT- 1 INHIBITORS USEFUL FOR THE TREATMENT OF OBESITY - Patent application
0114] Compounds of formula (I): [0115] Methyl 3-methyl-2-(5-(4-(3-(3-(trifluoromethyl)phenyl)ureido)phenyl)oxazole-2-ca- rboxamido)butanoate, [0116] 3-Methyl-2-(5-(4-(3-(3-(trifluoromethyl)phenyl)ureido)phenyl)oxazole-2-ca- rboxamido)butanoic acid, [0117] Methyl 2-(5-(4-(3-(2-chlorophenyl)ureido)phenyl)oxazole-2-carboxamido)-3-methylb- utanoate, [0118] 2-(5-(4-(3-(2-Chlorophenyl)ureido)phenyl)oxazole-2-carboxamido)-3-methylb- utanoic acid, [0119] Methyl 2-(5-(4-(3-(3,4-dimethylphenyl)ureido)phenyl)oxazole-2-carboxamido)-3-met- hylbutanoate, [0120] 2-(5-(4-(3-(3,4-dimethylphenyl)ureido)phenyl)oxazole-2-carboxamido)-3-met- hylbutanoic acid, [0121] Methyl 2-(5-(4-(3-(4-chloro-2-phenoxyphenyl)ureido)phenyl)oxazole-2-carboxamido)- -3-methylbutanoate, [0122] 2-(5-(4-(3-(4-chloro-2-phenoxyphenyl)ureido)phenyl)oxazole-2-carboxamido)- -3-methylbutanoic acid, [0123] Methyl 3-methyl-2-(5-(4-(3-(3-(trifluoromethyl)phenyl)ureido)phenyl)thiazole-2-c- arboxamido)butanoate, [0124] ...
  http://www.patentsencyclopedia.com/app/20120214854
*  Advances in Lipid Methodology, Volume 3 by W. W. Christie - GO JAPAN Books
83. , Fahey,J. ,J. A, 704, 99-111 (1995). 84. N. , Lipids, 5, 353-358 (1970). 85. , Ferrato,F. , Chirality, 5, 24-30 (1993). 86. , Ransac,S. ,J. Biol. , 265, 20271-20276 (1990). 87. , Damiani,P. ,J. Am. Oil Chem. , 69, 552-556 (1992). 88. , J. Am. Oil Chem. , 42, 945-957 (1965). 89. Sempore,G. ,J. Am. Oil Chem. , 68, 702-709 (1991). 90. Sempore,G. ,J. , 547, 89-103 (1991 ). 91. G. ,J. , 557, 227-240 (1991). 36 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105. 106. 107. 108. 109. 110. Yang and Kuksis [110,112] have used the method for stereospecific analysis of menhaden oil and of the chylomicron triacylglycerols derived from it to propose a convergence of the phosphatidic acid and the monoacylglycerol pathways of triacylglycerol biosynthesis during fat absorption. Yang et al. [113] have employed the chiral-phase HPLC method to demonstrate that hepatic triacylglycerols are not directly transferred to VLDL but first undergo lipolysis to acylglycerols before being re-synthesised into ...
  http://gojapan.seamolec.org/index.php/read/advances-in-lipid-methodology-volume-3-the-oily-press-lipid-library
*  A922500 | Transferase inhibitor | Read Reviews & Product Use Citations
A 922500 is an inhibitor for human and mouse DGAT-1 with IC50 of 7 nM and 24 nM, respectively, good selectivity over related acyltransferases, hERG, a...Quality confirmed by NMR & HPLC. See customer reviews, validations & product citations.
  http://www.selleckchem.com/products/a-922500.html
*  SAUSA300 0162 - AureoWiki
Cell Wall and CapsuleCapsular and extracellular polysacchridesSerotype determining Capsular polysaccharide biosynthesis in Staphylococcus Capsular polysaccharide synthesis enzyme Cap5K ...
  http://aureowiki.med.uni-greifswald.de/SAUSA300_0162
*  Cyclic Ether Dgat1 Inhibitorscyclic Ether Dgat1 Inhibitors (Novartis Ag)
The invention relates to compounds of formula (I): useful for treating disorders mediated by acyl coA-diacylglycerol acyl transferase 1 (DGAT1), e.g. metabolic disorders. The invention also provides methods of treating such disorders, and compounds and compositions etc. for their treatment.
  http://www.freshpatents.com/-dt20150521ptan20150141474.php
*  DGAT2L4 purified MaxPab mouse polyclonal antibody (B01P) - (H00158835-B01P) - Products - Abnova
Mouse polyclonal antibody raised against a full-length human DGAT2L4 protein. DGAT2L4 (AAI53071.1, 1 a.a. ~ 333 a.a) full-length human protein. (H00158835-B01P) - Products - Abnova
  http://www.abnova.com/products/products_detail.asp?catalog_id=H00158835-B01P
*  E-mail a Wiley Online Library Link
The aim of this study is to present the accumulation of microbial oil for biodiesel feedstock from low cost feedstock. The authors demonstrated the overproduction of TAG from glycerol in engineered Saccharomyces cerevisiae via the glycerol-3-phosphate pathway by overexpressing the major TAG synthesis. The content of TAG in the engineered strain yielded 8.2% TAG, representing a 2.3-fold improvement compared to the wild-type strain. The results should allow a reduction of costs and a more sustainable production of biodiesel. ...
  http://onlinelibrary.wiley.com/emailArticleLink?doi=10.1002/bit.24623&issueDoi=10.1002/bit.v110.1
*  重组人DGAT2蛋白(ab112427)|Abcam中国
购买我们的重组人DGAT2蛋白。Ab112427为全长蛋白,在小麦胚芽中生产并经过SDS-PAGE, ELISA, Western blot实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
  http://www.abcam.cn/recombinant-human-dgat2-protein-ab112427.html
*  Euonymus obovatus
To prevent poaching, NIRMI does not provide the location of any endangered, threatened, or otherwise protected species found within any of our plots. All lists, charts, and calculations (richness, FQI, etc.) therefore exclude these species as well ...
  http://www.nirmi.org/?pageNav=species&number=2809
*  重组人MGAT1蛋白(ab116960)|Abcam中国
购买我们的重组人MGAT1蛋白。Ab116960为蛋白片段,在小麦胚芽中生产并经过Western blot, SDS-PAGE, ELISA实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
  http://www.abcam.cn/recombinant-human-mgat1-protein-ab116960.html
*  DGA noms quintet for ad kudos - Variety
The Directors Guild of America has tapped feature filmmaker David Fincher, documentarian Errol Morris and 14-time nominee Joe Pytka as finalists for its top commercial award.
  http://variety.com/2004/film/news/dga-noms-quintet-for-ad-kudos-1117898546/