Immune Responses to Autologous Langerhans-type Dendritic Cells Electroporated With mRNA Encoding a Tumor-associated Antigen in...
This study will see if altered dendritic cells will make T cells work against tumor cells. The dendritic cells will be made in ... A dendritic cell is another type of white blood cell. It has most, if not all, of the proteins needed to make T cells work to ... The dendritic cells will contain messenger-RNA (mRNA). Cells use mRNA to make proteins. The mRNA will be put into dendritic ... This mRNA will help the dendritic cell make a tumor antigen like what the cancer expresses. The dendritic cell can then put ...https://clinicaltrials.gov/ct2/show/record/NCT01456104
Anatomic localization of dendritic cells subsets and plasmacytoid-like Th1 T lymphocytes in rheumatoid synovium: correlation...
Anatomic localization of dendritic cells subsets and plasmacytoid-like Th1 T lymphocytes in rheumatoid synovium: correlation ... This suggests a role for chemokines in the migration of T lymphocytes and dendritic cells (DCs) leading to the local ... CCL20-producing cells were associated with CD1a+ immature DCs or IL-17-producing T cells, suggesting the contribution of CCL20 ... Close association between CCL19, 21, CCR7, and mature DCs or IL-17-producing T cells argues for a role for these chemokines in ...https://arthritis-research.biomedcentral.com/articles/10.1186/ar498
Arc/Arg3.1 governs inflammatory dendritic cell migration from the skin and thereby controls T cell activation | Science...
Research ArticleDENDRITIC CELLS. Arc/Arg3.1 governs inflammatory dendritic cell migration from the skin and thereby controls T ... Dendritic cell migration reform. Dendritic cells are the messengers of the immune system, transporting antigens from sites of ... that is expressed in migratory dendritic cells in the skin. Arc/Arg3.1 regulates cytoskeletal changes in dendritic cells, ... Arc/Arg3.1 governs inflammatory dendritic cell migration from the skin and thereby controls T cell activation ...http://immunology.sciencemag.org/content/1/3/eaaf8665.editor-summary
DC (Dendritic-Cell) Isolation - Page 2 - Immunology - BioForum
Dendritic-Cell) Isolation - posted in Immunology: Hello Friends,I have just entered into immunology research and I need to know ... Hello! I have been isolating dendritic cells from a mouse spleen and the past three experiements my cells have died by the end ... cells is 1 million cell/ml DC medium and I replace half of the medium every 3 days.. While working with fresh PBMC of Buffy ... DC (Dendritic-Cell) Isolation. Started by Dirk Benke, Mar 13 2002 10:18 AM ...http://www.protocol-online.org/forums/topic/1390-dc-dendritic-cell-isolation/page-2
T cell stimulatory capacity of DCs cultured with PGE2. | Open-i
T cell stimulatory capacity of DCs cultured with PGE2. Day-5 DCs were recultured in the absence or presence of PGE2 (10 μM), ... Prostaglandin E2 and tumor necrosis factor alpha cooperate to activate human dendritic cells: synergistic activation of ... Prostaglandin E2 and tumor necrosis factor alpha cooperate to activate human dendritic cells: synergistic activation of ... After 48 h the cells were washed, irradiated, and DCs were used as stimulators of allogeneic T cell proliferation. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199106_JEM.971297f5&req=4
Mimicking the immunoregulatory properties of primary dendritic cells in vitro − A rational approach to design a test system. |...
Mimicking the immunoregulatory properties of primary dendritic cells in vitro − A rational approach to design a test system.. ...https://www.madforcancer.lu.se/carl-borrebaeck-0/publication/b2711bd2-5b58-4468-a9d1-9a889780d7da
Dendritic Cell Therapy - Emerging Treatment for Cancer
Oncologist Sumana Prem Kumar discusses Dendritic Cell Therapy, the Personalized Cancer Immunotherapy to treat cancer, which is ... Dendritic Cell Vaccine. Dendritic Cell Vaccine or Dendritic Cell Therapy (DCT) is an antigen extracted from the cancerous tumor ... About Dendritic Cell therapy Dendritic cells are found in the blood stream and are a unique set of antigen-producing cells that ... The neutrophils cells were then directed to the dormant dendritic cells. The dormant cells now activated, attacked the cancer ...http://www.medindia.net/news/Interviews/dendritic-cell-therapy-emerging-treatment-for-cancer-107157-1.htm
Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells | Journal of Cell Science
Dendritic cells (DCs) are potent antigen-presenting cells endowed with the unique ability to initiate adaptive immune responses ... The splicing co-factor Barricade/Tat-SF1 is required for cell cycle and lineage progression in Drosophila neural stem cells. ... Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells ... Serotonin receptor 5-HT7 regulates morphology and migratory properties of dendritic cells ...http://jcs.biologists.org/content/early/2015/06/16/jcs.167999
Helicobacter pylori induces human dendritic cell maturation and duodenal ulcer promoting gene a (dupA) enhances dendritic cell...
Dendritic cells are antigen-presenting cells with a unique ability to initiate a primary immune response from naïve T-cells. We ... Helicobacter pylori induces human dendritic cell maturation and duodenal ulcer promoting gene a (dupA) enhances dendritic cell ... Helicobacter pylori induces human dendritic cell maturation and duodenal ulcer promoting gene a (dupA) enhances dendritic cell ... Methods Human dendritic cells were derived from circulating monocytes from healthy donors. These cells were co-cultured with H ...http://gut.bmj.com/content/60/Suppl_1/A54.2
HKU Scholars Hub: BCG promotes cord blood monocyte-derived dendritic cell maturation with nuclear Rel-B up-regulation and...
BCG promotes cord blood monocyte-derived dendritic cell maturation with nuclear Rel-B up-regulation and cytosolic IκB α and β ... BCG promotes cord blood monocyte-derived dendritic cell maturation with nuclear Rel-B up-regulation and cytosolic IκB α and β ... Article: BCG promotes cord blood monocyte-derived dendritic cell maturation with nuclear Rel-B up-regulation and cytosolic IκB ... little is known about the initiation of response in neonatal dendritic cells (DCs) to BCG. To address this issue, the ...http://hub.hku.hk/handle/10722/170327
Frontiers | Paradigm Shift in Dendritic Cell-Based Immunotherapy: From in vitro Generated Monocyte-Derived DCs to Naturally...
... focused on vaccines that make use of primary dendritic cells. Though rare in the blood, these naturally circulating dendritic ... focused on vaccines that make use of primary dendritic cells. Though rare in the blood, these naturally circulating dendritic ... Upcoming treatment paradigms aim to include several primary dendritic cell subsets in a single vaccine as preclinical studies ... Upcoming treatment paradigms aim to include several primary dendritic cell subsets in a single vaccine as preclinical studies ...https://www.frontiersin.org/articles/10.3389/fimmu.2014.00165/full
Cell surface marker analysis of mouse thymic dendritic cells - Ardavin - 1992 - European Journal of Immunology - Wiley Online...
Thymic dendritic cells are negative for the stem cell antigen-2 (Sca-2), the B cell-specific form of CD45 (B220), the mouse ... The effects of IL-2 and Treg cells on dendritic cell homeostasis are mediated indirectly via activation of conventional T cells ... Cell surface marker analysis of mouse thymic dendritic cells. Authors. *. Carlos Ardavin,. Corresponding author. *Walter and ... Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase ...http://onlinelibrary.wiley.com/doi/10.1002/eji.1830220334/abstract
Help: Mouse Dendritic Cells preparation
... iddavis at vms.cis.pitt.edu iddavis at vms.cis.pitt.edu Fri Oct 25 11:17:44 EST 1996 * ... 2. Also how many DC needed to be added to see some IL-2/IFN production , by T cells (for 1x10 5 T cells) ? It depends on your ... I wouldn't go above a DC:T cell ratio of 1:4 at the most. Hope this helps. Ian Davis iddavis at vms.cis.pitt.edu University of ...http://www.bio.net/bionet/mm/immuno/1996-October/008840.html
Human dendritic cell subsets.
Dendritic cells are highly adapted to their role of presenting antigen and directing immune responses. Developmental studies ... dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong TH17/TH1 T-cell responsesJ Allergy Clin ... "Dermal Dendritic Cells" comprise two distinct populations: CD1+ dendritic cells and CD209+ macrophagesJ Invest DermatolYear: ... Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated ...http://www.biomedsearch.com/nih/Human-dendritic-cell-subsets/23621371.html
Analysis of Dendritic Cells at the Genetic Level | SpringerLink
... we have used two approaches to search at the genetic level for molecules which are specifically expressed by these cells. First ... To increase our understanding of dendritic cell (DC) function ... Dendritic Cell cDNA Library Mannose Receptor Dendritic Cell ... 1997) Analysis of Dendritic Cells at the Genetic Level. In: Ricciardi-Castagnoli P. (eds) Dendritic Cells in Fundamental and ... Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after ...https://link.springer.com/chapter/10.1007/978-1-4757-9966-8_73
Cutaneous Dendritic Cells Promote Replication of Immunodeficiency Viruses | SpringerLink
Cutaneous Dendritic Cells Promote Replication of Immunodeficiency Viruses. In: Ricciardi-Castagnoli P. (eds) Dendritic Cells in ... Dendritic Cells in Fundamental and Clinical Immunology pp 395-399 , Cite as ... are similar to the skin in histology and dendritic cell [DC] content. Since large amounts of fresh skin are more readily ... Hirsch, V., Riedel, N. and Mullins, J. I. Cell 49, 307-319 (1987).CrossRefGoogle Scholar ...https://link.springer.com/chapter/10.1007/978-1-4757-9966-8_64
Dendritic cells in herpesvirus infections | Frontiers Research Topic
Immature myeloid DC such as Langerhans cells (LC) and dermal DC are strategically located in the skin and mucosae, where they ... where they stimulate naïve T and B cells to proliferate and differentiate into CD8+ or CD4+ T lymphocytes, and into antibody- ... producing plasma cells. Plasmacytoid DC, by contrast, are mostly found in blood, where they exert potent anti-viral functions ... are a heterogeneous family of innate immune cells with crucial functions in host defense against invading pathogens, including ...https://www.frontiersin.org/research-topics/2189/dendritic-cells-in-herpesvirus-infections
Dendritic Cells, Viruses, and the Development of Atopic Disease
... Jonathan S. Tam and Mitchell H. Grayson ... Dendritic cells are important residents of the lung environment. They have been associated with asthma and other inflammatory ... Recent studies have begun to show the critical importance of the dendritic cell in this process. This paper focuses on these ... In addition to their antigen-presenting functions, dendritic cells have the ability to modulate the lung environment to promote ...https://www.hindawi.com/journals/ja/2012/936870/abs/
Immunity and Tolerance Induced by Intestinal Mucosal Dendritic Cells
... biological response modifiers and the family of cell adhesion-promoting molecules. ... Julio Aliberti, "Immunity and Tolerance Induced by Intestinal Mucosal Dendritic Cells," Mediators of Inflammation, vol. 2016, ... Immunity and Tolerance Induced by Intestinal Mucosal Dendritic Cells. Julio Aliberti Division of Immunobiology, Cincinnati ...https://www.hindawi.com/journals/mi/2016/3104727/cta/
Simian Immunodeficiency Virus Interactions with Macaque Dendritic Cells | SpringerLink
... dendritic cell (DC)-mediated simian immunodeficiency virus (SIV) transmission, (2) role of DCs in innate and adaptive immunity ... Dendritic cells during infection with HIV-1 and SIV. In: Lotze MT, Thomson AW (eds) Dendritic cells, chapter 35, 2nd edn. ... Hu J, Miller CJ, O'Doherty U, Marx PA, Pope M (1999) The dendritic cell-T cell milieu of the lymphoid tissue of the tonsil ... Lore K (2004) Isolation and immunophenotyping of human and rhesus macaque dendritic cells. Methods Cell Biol 75:623-642PubMed ...https://link.springer.com/chapter/10.1007/978-1-4614-4433-6_6
Some interfaces of dendritic cell biology: Ingenta Connect
Some interfaces of dendritic cell biology. APMIS 2003;111:675-97. The field of dendritic cell (DC) biology is robust, with ... regulatory T cells, peripheral T cell deletion), not just the initial priming or induction of T cell-mediated immunity, which ... DCs are now known to influence many different classes of lymphocytes (B, NK, NKT) and many types of T cell responses (Th1/Th2, ... A third interface is with cell biology. This is a critical discipline to understand at the subcellular and molecular levels the ...http://www.ingentaconnect.com/content/mksg/apm/2003/00000111/F0020007/art00002
Modified Dendritic Cells Induce Immune Response | Cancer Network
Such cells were shown to induce a significant and therapeutic tumor-specific immune response in an animal model. 1 ... PITTSBURGH-University of Pittsburgh researchers have shown that immature dendritic cells can be genetically modified to serve ... First, the dendritic cells, a type of antigen-presenting cell, were. injected directly into tumor tissue in mice. Then, Dr. ... cells directly into tumor tissue. The secretion of IL-12 by the. genetically modified dendritic cells was found to further ...http://www.cancernetwork.com/articles/modified-dendritic-cells-induce-immune-response
Sensors | Free Full-Text | Dendritic Cells as Danger-Recognizing Biosensors
Since DCs possess the intrinsic capacity to polarize CD4+ helper cells, it is critical to understand the immunological roles of ... are antigen presenting cells that are characterized by a potent capacity to initiate immune responses. DCs comprise several ... DCs become mature and subsequently present antigens to CD4+ T cells. ... Dendritic cells (DCs) are antigen presenting cells that are characterized by a potent capacity to initiate immune responses. ...http://www.mdpi.com/1424-8220/9/9/6730
Gating of dendritic cells - Flow Cytometry - BioForum
When we prepare cells for FACS analysis, we always try to disaggregate dendritic cells, which are notoriously sticky (e.g. with ... I have a question regarding FACS analysis of dendritic cells. I understand that aggregates need to be excluded during analysis ... Gating of dendritic cells - posted in Flow Cytometry: ... Gating of dendritic cells. Started by xinmimi77, Mar 25 2013 08 ... When we prepare cells for FACS analysis, we always try to disaggregate dendritic cells, which are notoriously sticky (e.g. with ...http://www.protocol-online.org/forums/topic/28863-gating-of-dendritic-cells/
Positioning of follicular dendritic cells within the spleen controls prion neuroinvasion. - Zurich Open Repository and Archive
Here we show that ablation of the chemokine receptor CXCR5 juxtaposes follicular dendritic cells (FDCs) to major splenic nerves ... Here we show that ablation of the chemokine receptor CXCR5 juxtaposes follicular dendritic cells (FDCs) to major splenic nerves ... Positioning of follicular dendritic cells within the spleen controls prion neuroinvasion. Nature, 425(6961):957-962. ... Both immune cells and nerves are involved in pathogenesis, but the mechanisms of prion transfer from the immune to the nervous ...http://www.zora.uzh.ch/id/eprint/1891/