Assessment of Nili-Ravi buffalo (Bubalus bubalis) semen by MTT reduction assay
IQBAL, M. et al. Assessment of Nili-Ravi buffalo (Bubalus bubalis) semen by MTT reduction assay. S. Afr. j. anim. sci. [online]. 2009, vol.39, n.5, pp.294-300. ISSN 2221-4062.. MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay is commonly used to validate the viability of metabolically active cells. The study was conducted to examine and validate the MTT test to assess the sperm viability of Nili-Ravi buffalo bulls and compare the efficiency of the test with the supra-vital staining technique (eosin-nigrosine) and hypo-osmotic swelling test. Fresh semen samples from breeding Nili-Ravi buffalo bulls (n = 20) were collected using an artificial vagina. After assessing the quality of semen for normal parameters, the MTT assay was carried out in phosphate buffer saline. Results revealed a high significant correlation (r = 0.995) between the viability of sperm and the rate of reduction of MTT. The other proportions of some semen samples showed a weak relationship between the ...http://www.scielo.org.za/scielo.php?script=sci_abstract&pid=S0375-15892009000500069&lng=es&nrm=iso&tlng=en
Variasi Panjang Kaki Kerbau Lumpur (Bubalus bubalis) di Kabupaten Jembrana Bali: Panjang Humerus - Metacarpus dan Femur â€"...
Kerbau rawa atau kerbau lumpur (Bubalus bubalis) merupakan hewan ternak yang cukup potensial dikembangkan di daerah pertanian. Suatu keunikan terdapat di Kabupaten Jembrana adalah kerbau secara berpasangan menarik cikar kemudian di adu lari cepat dengan pasangan-pasangan kerbau yang lain. Peristiwa adu lari cepat pasangan kerbau jantan tersebut dinamakan makepung. Penelitian dilakukan di Kabupaten Jembrana Bali pada bulan januari 2015 untuk meneliti variasi panjang kaki (humerus-metacarpal dan femur-metatarsal) kerbau lumpur. Penelitian menggunakan kerbau lumpur jantan dewasa yang digunakan dalam kegiatan makepung dengan jumlah 63 ekor. Pada blok barat terdapat 37 ekor dan blok timur 26 ekor. Data yang dikumpulkan dianalisis dengan Uji-t (P â‰¥0,05). Hasil penelitian menunjukkan bahwa pada blok barat, panjang kaki depan atas (scapula-humeri sampai carpi-antebracii) merupakan ukuran kaki yang paling seragam dan panjang kaki depan bawah (carpi-antebracii sampai metacarpi-phalangeal) merupakan ...http://erepo.unud.ac.id/11969/
Nematodes of the small intestine of African buffaloes, Syncerus caffer, in the Kruger National Park, South Africa
Communication. Helminths of African buffaloes, Syncerus caffer, have not been well documented compared with many southern African ungulates (Boomker et al. 1996). Although at least 25 nematode species have been recorded in the gastro-intestinal tract (GIT) of buffaloes across Africa (with 13 of these recorded in South Africa), quantitative studies to date have used small sample sizes, such as four from the Kruger National Park (KNP), South Africa, and four from the Hluhluwe-iMfolozi Game Reserve (HiMGR), South Africa (Boomker et al. 1996). The average burdens from these two collections were 1086 and 2096 worms, respectively. Such numbers appear low for an animal the size of a buffalo and the general impression is that buffaloes do not harbour significant intestinal nematode burdens (Boomker et al. 1996). Basson et al. (1970) examined the carcasses of 100 buffaloes in the KNP for parasitic and other diseases and recorded nine helminth species ...http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652013000100020&lng=pt&nrm=iso
Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina | BMC...
In the present study, we describe the isolation of BPIV3 from two water buffaloes with respiratory and reproductive signs. The virus was isolated from nasal and vaginal swabs from two out of twelve animals with reproductive and respiratory signs studied. To our knowledge little is known about the pathogenesis of this virus in buffalo. Should there be a syndrome similar to that present in cattle where the virus is transiently excreted , the unsuccessful virus isolation from the then remaining symptomatic animals could be related to the time point of sampling, which could have taken place at the end of the acute infection.. The IF assay showed strong and specific binding of anti-BPIV3 antibodies to cells infected with these three positive clinical isolates. Sera from buffaloes were able to neutralize the activity of the 7 N, 7 V and 2 V isolates and that of the reference bovine virus (BPIV3 strain SF), but the neutralization titers against the latter were lower than ...https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-83
Caudal Extradural Analgesia with Lidocaine, Xylazine, and a Combination of Lidocaine and Xylazine in the Iranian River Buffalo...
Objective- To compare the time of onset, duration of action and the extent of analgesia produced by a lidocaine/xylazine combination with that produced by lidocaine and xylazine alone after injection into the caudal extradural space of the Iranian river buffalo. Design- Observer-blind prospective descriptive trial, Latin square design. Animals- Eleven adult (aged over 2 years) female non-gravid healthy Iranian river buffaloes (Bubalus bubalis), weighing from 450 to 650 kg. Procedure- Caudal extradural analgesia was achieved in buffaloes on 3 occasions at 14-day intervals by injection of 2% lidocaine (L; 0.22 mg kg-1 ), 2% xylazine (X; 0.05 mg kg-1 ), and a combination of 2% lidocaine(0.22 mg kg-1 ) / 2% xylazine (LX; 0.05 mg kg-1 ) in a Latin square design. Analgesia was determined by the lack of response to pin-prick and haemostat pressure in the skin of the caudal areas. Results- Onset of analgesia for X was significantly longer (5.5 ± 0.7 minutes) than ...http://www.ivsajournals.com/article_3293.html
Buffalo Bisons - Wikipedia
Buffalo Bisons - squadra attiva nella International League Buffalo Bisons - squadra attiva nella National League dal 1879 al 1885 Buffalo Bisons - squadra attiva nella Players League nel 1890. Buffalo Bisons - squadra attiva nella International League e Eastern League dal 1886 al 1970 Buffalo Bisons - squadra attiva nella International Association (campione nel 1878) Buffalo Bisons - squadra attiva nella All-America Football Conference nel 1946 che disputò le stagioni dal 1947 al 1949 col nome di Buffalo Bills Buffalo Bisons - squadra attiva nella National Football League dal 1924 al 1929 e che disputò altre stagioni coi nomi di Buffalo All-Americans e Buffalo Rangers. Buffalo Bisons - squadra attiva nella National Basketball League dal 1937 al 1938. Buffalo Bisons - squadra attiva nella National Basketball League nel 1946 e rinominatasi Tri-Cities Blackhawks dopo il trasferimento a campionato 1946-47 in corso. Buffalo Bisons - squadra attiva nella International Hockey League Buffalo Bisons - ...https://it.wikipedia.org/wiki/Buffalo_Bisons
Download Bubalis, asian, ruminant, south, klaus-werner friedrich
Bubalis: 18 assigned downloads, like Water Buffaloes Bubalus bubalis, Kompong Thom Province, Cambodia, Southeast Asia from stock-photoshttps://www.tradebit.com/tagworld.php/bubalis
Concerted evolution of α globin genes in Podolian cattle (Bos taurus primigenius) and river buffalo (Bubalus bubalis)? |...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...http://www.biochemsoctrans.org/content/28/5/A186.3
Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park...
Introduction. Bovine tuberculosis (BTB) is a chronic, contagious disease caused by Mycobacterium bovis, a Gram-positive acid-fast bacterium. This pathogen is particularly noted for its diverse host tropism, which includes humans, livestock and several wildlife species. The first report of BTB in cattle in South Africa was made by Hutcheon (1880), whilst it was first diagnosed in African buffaloes (Syncerus caffer) in the Hluhluwe-iMfolozi Park (HiP) in 1986 (Jolles 2004). Spillover of BTB to other species has been documented (Michel et al. 2006; Michel et al. 2009). The HiP is situated in KwaZulu-Natal, South Africa and the area of 100 000 ha is almost entirely surrounded by communal farmland and livestock. It is the fourth largest game reserve in South Africa and has a buffalo population of approximately 3500 heads. The molecular characterisation of M. bovis is essential in understanding disease transmission between species and the spatial distribution of this infection in wildlife ...http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652011000100006&lng=en&nrm=iso&tlng=en
The osteology of the cranial and facial bones of the savannah buffalo Syncerus caffer caffer (Sparrman, 1779)
Zoologists classify the savannah buffalo under the Bovini Tribe. Osteologically, the skull differs from that of the water buffalo of Asia, inter alia, in that the vomer does not articulate with the palatine part of the osseus palate. This gross anatomical study gives a detailed description of all the bones of the skull, mandible and hyoid apparatus of the savannah buffalo Syncerus caffer caffer (Sparrman, 1779). These bones are similar in many respects to that of the domestic bovine. However, due to the robustness of the buffalo skull, many aspects pertaining to bones or parts of bones that are different or more pronounced, are of anatomical importance. The sum-total effect of all these features, gives the skull its typical macro-morphology that differentiates it clearly from the other genera in the Bovini Tribe. The more important characteristics that were found, are the following: ,ul, ,ol,1. The skull of young animals retains basic embryonic reshaping potential till quite late in life. It can ...http://repository.up.ac.za/handle/2263/30244
Cloned buffalo (Bubalus bubalis) embryos from adult cumulus cells and cytoplasts prepared by demecolcine-assisted enucleation...
We report a simplified chemically-enhanced enucleation of in vitro matured buffalo oocytes for generating cytoplasts for producing nuclear transfer (NT) embryo production. Oocytes aspirated from the ovaries from abattoir were subjected to in vitro maturation for 22h. In the first experiment, the in vitro matured (IVM) oocytes (22h) were denuded and treated with demecolcine (0.50 µg/ml IVM medium) for additional 2h. Oocytes exhibiting characteristic extrusion cones were enucleated using traditional or standard micromanipulator-guided enucleation. In the second experiment, the IVM (22h) oocytes were denuded and treated with demecolcine (0.50 µg/ml IVM medium) for 2h. The zona pellucidae of denuded oocytes were then removed using prteinase K (2µg/ml in M-199 with 0.4% FBS). Cytoplasts were prepared by micromanipulator-free or manual bisection of extrusion cones bearing zona-free oocytes. Chemically-assisted cytoplasts formation efficiency was found to be 59.99% using micromanipulator-guided ...http://inpressco.com/effect-of-cutting-parameters-on-mrr-and-surface-finish-prediction-model/
Isolation of pestes des petits ruminants virus from an outbreak in Indian buffalo (Bubalus bubalis) | Veterinary Record
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center's RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...http://veterinaryrecord.bmj.com/content/141/22/573
Cryopreservation of Zona-Free Cloned Buffalo (Bubalus Bubalis) Embryos: Slow Freezing vs Open-Pulled Straw Vitrification.
This study was carried out to compare the post-thaw cryosurvival rate and the level of apoptosis in vitro produced zona-free cloned buffalo blastocysts subjected to slow freezing or vitrification in open-pulled straws (OPS). Zona-free cloned embryoshttp://www.biomedsearch.com/nih/Cryopreservation-Zona-Free-Cloned-Buffalo/23281817.html
Amazing African Animals: The Cape African Buffalo great Bulls with great Horns
In many parts of the African Savannah the African Buffaloes,are mostly found in many parts of the African forests, the beautiful African woodlands and and the vast African Savannah which is rich with its great flora and fauna of Africa. These beautiful dangerous African buffaloes are mostly located near the swamps which are flooded with the dangerous Nile crocodiles and, many floodplains with Africa's most dangerous big five greatest dangerous animals of the world with many of the dangerous venomous snakes living along the grasslands of Africa. All African Buffaloes like to live in their natural habitat just as many herbivorous that prefer ta dense cover of reeds and fresh grass to fill their big appetite. African Buffaloes depend on large quantities of water and hence, stay close to the perennial sources of the same ...http://africansanimals.blogspot.com/2012/08/the-cape-african-buffalo-great-bulls.html
Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis...
PAULIN, Lília Márcia Silva et al. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis). Cienc. Rural [online]. 2012, vol.42, n.9, pp.1621-1626. Epub 14-Ago-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000070.. The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement ...http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-84782012000900017&lng=es&nrm=iso&tlng=en
In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell.
Spermatogonial stem cells (SSCs) have the unique ability both to self-renew and to produce progeny that undergo differentiation to spermatozoa. The present study has been carried out to develop a method to purify and enrich the pure populations of sphttp://www.biomedsearch.com/nih/In-vitro-culture-morphological-characterization/23151879.html
Gousiekte in African buffalo (Syncerus caffer)
INTRODUCTION Three African buffalo (Syncerus caffer) that died after capture and translocation showed macroscopic and microscopic lesions of cardiomyopathy. The commonest cause of cardiomyopathy in freeranging ruminants in southern Africa is gousiekte, a sub-acute to chronic intoxication by members of the plant family Rubiaceae that contain the polyamine pavetamine4. The previous assumption that the myocardial lesions described in the literature are pathognomonic has been discarded5, but a diagnosis of gousiekte is justified in cases of cardiomyopathy where one of the plants known to cause the condition is present. This paper describes an outbreak in Mutirikwe Recreational Park in Zimbabwe and considers possible epidemiological factors responsible for the outbreak. MUTIRIKWE RECREATIONAL PARK Mutirikwe (Kyle) Recreational Park in southern Zimbabwe (20º10'S, 31º01'E) includes a wildlife area of approximately 3800 ha enclosed by Lake Mutirikwe and a game-proof fence. In the north of the Park the ...http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S1019-91282010000300009&lng=en&nrm=iso&tlng=en
Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview | Journal of Venomous Animals and...
In Brazil, the following four buffalo breeds are recognized by the Brazilian Association of Buffalo Breeders: mediterrâneo, murrah, jafarabadi (river buffalo) and carabao (swamp buffalo). The murrah breed, Bubalus bubalis, of Indian origin has been raised at the Lageado Experimental Farm, UNESP campus in Botucatu, for more than 30 years .. In order to ensure that this bioproduct contains no substance foreign to the human body, it is necessary to select and certify the donors. Therefore, sanitary management is mandatory for good economic results, which includes the following actions: annual vaccination against foot-and-mouth disease, brucellosis and rabies; systematic deworming; measures for hygiene and asepsis; practices of isolation and quarantine; protection of animals against vectors of transmissible diseases; diagnostic serological tests against brucellosis and leptospirosis; an annual hypersensitivity test against tuberculosis (tests of tuberculinization and of Mantoux or PPD); as well ...https://jvat.biomedcentral.com/articles/10.1186/s40409-017-0109-8
Influence of Crude Protein and Energy Level on Feed Intake, Ruminal Ammonia Nitrogen, and Methylglyoxal Production in Swamp...
The present experiments were conducted to investigate the effect of protein (CP) and energy levels in concentrate supplementation on feed intake, ruminal ammonia nitrogen (NH3-N) and methylglyoxal (MG) production in swamp buffaloes (Bubalus bubalis) fed rice straw based diet. Eight Thai rumen fistulated male swamp buffaloes, about 4 years old with body weight (BW) of 381±10 kg, were randomly assigned to four dietary treatments in two consecutive 4 × 4 Latin square design experiments. Four buffaloes in first square received four different concentrate supplementations containing different CP levels at 92, 124, 181 and 219 g/kg while other four in the second square received different concentrate containing different total digestible nutrient (TDN) levels at 740, 761, 806 and 843 g/kg, respectively. All buffaloes were fed concentrate mixtures at 10 g/kg BW and rice straw was offered ad libitum. Under this investigation, the result revealed that ...https://www.semanticscholar.org/paper/Influence-of-Crude-Protein-and-Energy-Level-on-Fee-Chanthakhouna-Wanapata/0b3472bc4e3b31fc4612b1c9ae5c200a205fc5f5
2015 Buffalo Bulls football team - Wikipedia
The 2015 Buffalo Bulls football team represented the University at Buffalo in the 2015 NCAA Division I FBS football season. The Bulls were led by first-year head coach Lance Leipold. The team played their home games at University at Buffalo Stadium and competed as a member of the East Division of the Mid-American Conference. They finished the season 5-7, 3-5 in MAC play to finish in fourth place in the East Division. Schedule Source: Week One: Albany Great Danes at Buffalo Bulls - Game summary at UB Stadium, Amherst, NY Date: September 5 Game time: 3:30 PM EDT Game weather: Weather: Partly Cloudy • Temperature: 80 • Wind: SW 5-10 Game attendance: 20,872 Referee: Tom Stapleton TV: ESPN3 Final Stats In their first game of the season, the Bulls won, 51-14 over the Albany Great Danes. Week Two: Buffalo Bulls at Penn State Nittany Lions - Game summary at Beaver Stadium, University Park, PA Date: September 12 Game time: 12:00 PM EDT Game weather: Weather: Rainy • Temperature: 61-64 • Wind: SE ...https://en.wikipedia.org/wiki/2015_Buffalo_Bulls_football_team
Nutrient intake, acid base status and weight gain in water buffalo calves fed different dietary levels of sodium bicarbonate
SARWAR, M; SHAHZAD, M.A; NISA, M e AMJAD, S. Nutrient intake, acid base status and weight gain in water buffalo calves fed different dietary levels of sodium bicarbonate. S. Afr. j. anim. sci. [online]. 2011, vol.41, n.2, pp.94-103. ISSN 2221-4062.. The impact of different dietary levels of sodium bicarbonate (NaHCO3) on nutrient intake, acid-base status, nitrogen balance and weight gain was examined in growing male buffalo (Bubalus bubalis) calves exposed to hot summer conditions. In a complete randomized block design 60 animals of similar age and weight were divided into five treatment groups of 12 per group. Five isonitrogenous and isoenergetic diets were formulated and randomly allocated to a treatment group. The control diet (0SB) contained no NaHCO3, while diets 4SB, 8SB, 12SB and 16SB contained 0.4, 0.8, 1.2 and 1.6% NaHCO3, respectively. An increase in nutrient intake was recorded with increasing dietary NaHCO3 level while the reverse was true for nutrient digestibility. Calves fed the ...http://www.scielo.org.za/scielo.php?script=sci_abstract&pid=S0375-15892011000200004&lng=pt&nrm=iso
Modelling population dynamics of the African Buffalo Syncerus caffer using data on population estimates, harvest and rainfall...
Knowledge on the population dynamics of African buffalo is a prerequisite for developing its successful harvest strategy. Acquiring such knowledge requires among others the use of population models which reveals important demographic information and suggests some management actions to be taken. This study aims at developing an understanding on how to take population estimates and influences of rainfall into account when developing harvest strategy. I hypothesize that annual quota would be influenced by population estimates and that population estimates would be influenced by rainfall. I used data on the buffalo population estimates, annual quota, harvest, and rainfall as well as survival and fecundity rates obtained from different sources. The study employs the use of the package Popbio in the statistical program R, to construct and analyze an age-specific Leslie matrix population model. Tracing study on the increase phases of the Selous - Mikumi population estimates reveals that high annual ...https://brage.bibsys.no/xmlui/handle/11250/132243
Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah...
Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah Buffalo (Bubalus bubalis - Buffalo;Follicles;Ovum Pick-up;TVOR;http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1DMBP_2005_v18n5_632
DNA-based vaccine protects against zoonotic schistosomiasis in water buffalo
Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option would be development of vaccines that reduce transmission of S. japonicum from bovines to replace bovine chemotherapy. We performed two randomized double blind trials in water buffaloes to determine if DNA vaccines encoding triose-phosphate isomerase (SjCTPI), or the tetraspanin 23 kDa integral membrane protein (SjC23), alone or fused to bovine heat shock protein 70 (Hsp70) could induce a level of immunity conducive to long-term sustainable control. Groups of water buffaloes (15/group) ...https://research-repository.griffith.edu.au/handle/10072/40516
Clinicophysiological and haemodynamic effects of fentanyl with xylazine, medetomidine and dexmedetomidine in isoflurane...
SINGH, Gyan D. et al. Clinicophysiological and haemodynamic effects of fentanyl with xylazine, medetomidine and dexmedetomidine in isoflurane-anaesthetised water buffaloes (Bubalus bubalis). J. S. Afr. Vet. Assoc. [online]. 2013, vol.84, n.1, pp.1-11. ISSN 2224-9435.. The present study was undertaken to investigate the sedative, analgesic and clinical effects of xylazine, medetomidine and dexmedetomidine with fentanyl as pre-anaesthetics in water buffaloes and to compare the dose-sparing effect of xylazine, medetomidine and dexmedetomidine on thiopental for induction and isoflurane for maintenance of anaesthesia in water buffaloes. Six male water buffaloes randomly received intravenous fentanyl (5.0 pg/kg body weight) and xylazine (0.05 mg/kg body weight), fentanyl (5.0 pg/kg body weight) and medetomidine (2.5 pg/kg body weight), fentanyl (5.0 pg/kg body weight) and dexmedetomidine (5.0 pg/kg body weight) at weekly intervals in groups I1, I2 ...http://www.scielo.org.za/scielo.php?script=sci_abstract&pid=S1019-91282013000100003&lng=en&nrm=iso&tlng=en