The Lysosomal-Mitochondrial Axis Theory of Apoptosis
... released lysosomal enzymes and/or prevents down-stream activation of cytosolic pro-apoptotic enzymes by released lysosomal ... which may dcstabilize lysosomal as well as mitochondrial membranes. Indeed, the specific inhibition of PLA2 by 4-bromophenacyl ... preventing further release of lysosomal enzymes and of mitochondrial pro-apoptotic proteins. ... intra-lysosomal oxidation leading to lysosomal rupture. Moderate lysosomal disruption induces apoptosis, while more extensive ...http://liu.diva-portal.org/smash/record.jsf?pid=diva2:248642
Lysosomal-associated membrane protein 2 isoforms are differentially affected in early Parkinson's disease | Garvan Institute of...
... as well as with the accumulation of cytosolic CMA substrate proteins. Our data show that LAMP2 protein isoforms are ... lysosomal-associated membrane protein (LAMP) 2A, although whether LAMP2A is the only LAMP2 isoform affected by PD is unknown. ... lysosomal-associated membrane protein (LAMP) 2A, although whether LAMP2A is the only LAMP2 isoform affected by PD is unknown. ... as well as with the accumulation of cytosolic CMA substrate proteins. Our data show that LAMP2 protein isoforms are ...https://www.garvan.org.au/research/publications/13238
HGF-induced invasion by prostate tumor cells requires anterograde lysosome trafficking and activity of Na+-H+ exchangers |...
2001). Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 20, 683-693. ... These data suggest that EIPA may cause an increase of cholesterol in lysosomal membranes and this may activate ORP1L to induce ... 1989). Na+/H+ exchange and growth factor-induced cytosolic pH changes. Role in cellular proliferation. Biochem. Biophys. Acta ... Rab7 and its effector RILP (Rab7-interacting lysosomal protein) are known to play a role in the retrograde trafficking of late ...http://jcs.biologists.org/content/123/7/1151
Intrinsic fluorescence of the clinically approved multikinase inhibitor nintedanib reveals lysosomal sequestration as...
Costes SV, Daelemans D, Cho EH, Dobbin Z, Pavlakis G, Lockett S. Automatic and quantitative measurement of protein-protein ... There the drug in its neutral state is expected to diffuse freely across the plasma membrane. At the same time, disrupting ... lowering cytosolic drug concentrations and, thus, the FGFR inhibition-based cytotoxic potential. Our findings suggest that key ... Lysosomal sequestration of hydrophobic weak base chemotherapeutics triggers lysosomal biogenesis and lysosome-dependent cancer ...https://jeccr.biomedcentral.com/articles/10.1186/s13046-017-0592-3
Induction of Lysosomal Biogenesis in Atherosclerotic Macrophages Can Rescue Lipid-Induced Lysosomal Dysfunction and Downstream...
LAMP1 (lysosomal-associated membrane protein 1), a glycoprotein abundantly expressed on the lysosomal membrane, is a commonly ... The lysosome receives extracellular cargo (via endocytosis) and cytosolic material (via autophagy) for degradation. Failure of ... Atherogenic Lipids Increase Lysosomal Membrane Permeability. An intact lysosomal membrane is essential for the maintenance of a ... The accumulation of lysosomal free cholesterol can directly cause an increase in lysosomal membrane cholesterol content.38 ...http://atvb.ahajournals.org/content/34/9/1942
Crystal structure of the conserved domain of the DC lysosomal associated membrane protein: implications for the lysosomal...
Lysosomal membrane proteins have sorting signals in their cytosolic domains. Early endosomes acquire lysosomal proteins and an ... A comprehensive list of 45 lysosomal membrane proteins  was analysed with PFAM. Multidomain proteins were not found in this ... Crystal structure of the conserved domain of the DC lysosomal associated membrane protein: implications for the lysosomal ... Saftig P: Lysosomal membrane proteins. Lysosomes. Edited by: Saftig P. 2005, New York: Springer, 37-49.View ArticleGoogle ...https://0-bmcbiol-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1741-7007-10-62
Human Cytosolic beta-Glucosidase/GBA3 Antibody AF5969: R&D Systems
View Human Cytosolic beta-Glucosidase/GBA3 Antibody (AF5969) datasheet. Validated in IP, WB ... GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide. GBA2 encodes a ... CBG; CBGL1; CBGL1GLUC; Cytosolic betaGlucosidase; Cytosolic beta-Glucosidase; Cytosolic beta-glucosidase-like protein 1; EC 3.2 ... PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic beta ‑Glucosidase/GBA3 Antigen Affinity-purified Polyclonal ...https://www.rndsystems.com/products/human-cytosolic-beta-glucosidase-gba3-antibody_af5969
LAMP2 Gene - GeneCards | LAMP2 Protein | LAMP2 Antibody
Protein Coding), Lysosomal Associated Membrane Protein 2, including: function, proteins, disorders, pathways, orthologs, and ... Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Tuberculosis. GO annotations related to this ... Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; ... lysosomal associated membrane protein 2 *LAMP2. UniProtKB/Swiss-Prot Function: Plays an important role in chaperone-mediated ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=LAMP2&origene_rsshrna_func=3&rf=/home/genecards/current/website/carddisp.pl
Frontiers | GTPase of the Immune-Associated Nucleotide Protein 5 Regulates the Lysosomal Calcium Compartment in T Lymphocytes |...
Strikingly, TCR stimulation of rat, mouse and human T lymphocytes increased lysosomal calcium content. Overall, our findings ... Strikingly, TCR stimulation of rat, mouse and human T lymphocytes increased lysosomal calcium content. Overall, our findings ... T lymphocytes from Gimap5lyp/lyp rats carrying a recessive mutation in the GTPase of Immune-Associated Protein 5 (Gimap5) gene ... T lymphocytes from Gimap5lyp/lyp rats carrying a recessive mutation in the GTPase of Immune-Associated Protein 5 (Gimap5) gene ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00094/full
Chaperone-mediated autophagy - Wikipedia
... at the lysosomal membrane and thus, inhibit the CMA-mediated degradation of other cytosolic substrate proteins. Links between ... The proteins that are degraded through CMA are cytosolic proteins or proteins from other compartments once they reach the ... In these cases, the defect lies in the 'tight' binding to the lysosomal membrane of pathogenic proteins known to accumulate in ... 2005). "Unifying Nomenclature for the Isoforms of the Lysosomal Membrane Protein LAMP-2". Traffic. 6 (11): 1058-61. doi:10.1111 ...https://en.wikipedia.org/wiki/Chaperone-mediated_autophagy
Frontiers | Autophagosomal Protein Dynamics and Influenza Virus Infection | Immunology
... catabolic process leading to the lysosomal degradation of cytosolic proteins and organelles. However, it is also induced under ... Membrane dynamics, as well as protein-protein and protein-membrane interactions are particularly addressed. In addition, it ... Membrane dynamics, as well as protein-protein and protein-membrane interactions are particularly addressed. In addition, it ... However, it is also induced under stress conditions, remodeling the eukaryotic cell by regulating energy, protein and lipid ...https://www.frontiersin.org/articles/10.3389/fimmu.2012.00043/full
Antibodies | Free Full-Text | Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted...
... been developed in the last decades to overcome endosomal accumulation and subsequent lysosomal degradation of targeted protein- ... While numerous transport systems exist for ions and small molecules, the cytosolic uptake of larger biological molecules and in ... membrane barrier including the use of molecular ferries such as cell penetrating peptides or viral membrane fusion proteins, ... Inducing leakage of the plasma membrane is unfavorable since the target cell specificity mediated by the antibody would likely ...http://mdpi.com/2073-4468/2/2/209/htm
anti-Lysosomal-Associated Membrane Protein 1 (LAMP1) Antikörper</span>
Human Polyclonal LAMP1 Primary Antibody für ICC, IF - ABIN4330169 : Edwards, Rockx-Brouwer, Nair, Celli: Restricted cytosolic ... lysosome-associated membrane protein 1 , LAMP-1 , Lysosomal associated membrane protein 1 (120 kDa) , chromaffin granule- ... anti-Lysosomal-Associated Membrane Protein 1 Antikörper (LAMP1). Auf www.antikoerper-online.de finden Sie aktuell 515 Lysosomal ... lysosomal membrane glycoprotein 1 , lysosomal membrane glycoprotein A , lysosome-associated membrane glycoprotein 1 , ...http://www.antikoerper-online.de/autophagie-pathway-94/lamp1-antibody-846/
Golgi and Protein Processing
Rough ER - the cytosolic surface of the membranes have attached ribosomes that are synthesizing proteins for import into the ER ... figure shows the flow of proteins in the secretory, lysosomal and endocytotic pathways that will be considered later. Click on ... If these proteins are to be moved, they must be moved as part of membrane vesicles, and any enzymes that act on the proteins ... Would you expect to find disulfide bridges in the extracellular or in the cytosolic portion of membrane proteins? ...http://www.zoology.ubc.ca/~berger/B200sample/unit_8_protein_processing/golgi/lect28.htm
PSAP Gene - GeneCards | SAP Protein | SAP Antibody
Protein Coding), Prosaposin, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. ... prosaposin,lysosomal,sphingolipid hydrolase proactivator protein processed to SAPA,SAPB,SAPC,SAPD,ordered 5-,3 on the precursor ... Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Metabolism. GO annotations related to this gene ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=PSAP&keywords=GH10G071887&prefilter=genomic_location
Specific Targeting of the EBV Lytic Phase Protein BNLF2a to the Transporter Associated with Antigen Processing Results in...
1⇑A). To investigate whether BNLF2a is a cytosolic or a membrane-associated protein, we performed subcellular fractionation ... Enhanced lysosomal degradation is also effected by two β-herpesvirus gene products; the U21 protein of the human herpesvirus ... the membrane protein US6 inhibits TAP through its ER lumenal domain by interfering with ATP binding to the cytosolic nucleotide ... for the presence of the control transmembrane protein transferrin receptor (TfR; H68.4 mAb), the control cytosolic protein GFP ...http://www.jimmunol.org/content/182/4/2313.long
Microarray analysis in B cells among siblings with/without MS - role for transcription factor TCF2 | BMC Medical Genomics |...
... governed by the upregulated transcription factor 2 (TCF2) and the smaller network regulated by the downregulated CDC5L. No ... LYPLA3 (lysosomal phospholipase A2): Lysophospholipases are enzymes that act on biological membranes to regulate the ... lysosomal associated protein transmembrane 4 beta (LAPTM4B), mRNA phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 ... cytosolic phospholipase A2 levels were found to be low, possibly reflecting proteolysis or inactivation of enzyme activity in ...https://bmcmedgenomics.biomedcentral.com/articles/10.1186/1755-8794-1-2
... proteins that target particular steps in the standard Golgi → transport vesicle → vesicle/plasma membrane fusion trafficking ... Measurement and buffering of cytosolic Ca2+.. Receptor-triggered elevations in cytosolic [Ca2+] were assayed using fura 2 ... a substrate for lysosomal cathepsin C, to coordinately collapse lysosome integrity and repress the ATP release stimulated by ... After protein quantification, the frozen aliquots of cell lysate were rapidly thawed, and 0.75 mg/ml protein was used in each ...http://ajpcell.physiology.org/content/295/1/C231
Forms, Crosstalks, and the Role of Phospholipid Biosynthesis in Autophagy
... targeting the protein to the lysosome for degradation (d). The CMA-specific protein is unravelled at the lysosomal membrane by ... R. Sahu, S. Kaushik, C. C. Clement et al., "Microautophagy of cytosolic proteins by late endosomes," Developmental Cell, vol. ... The ADP bound form of Hsc70 then targets the HSPC to the lysosomal membrane. Transport of the protein substrate into the lumen ... F. A. Agarraberes and J. F. Dice, "A molecular chaperone complex at the lysosomal membrane is required for protein ...https://www.hindawi.com/journals/ijcb/2012/931956/
BLOC1S2 Gene - GeneCards | BL1S2 Protein | BL1S2 Antibody
Protein Coding), Biogenesis Of Lysosomal Organelles Complex 1 Subunit 2, including: function, proteins, disorders, pathways, ... The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos (By similarity). Component of the BLOC- ... Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus- ... Protein details for BLOC1S2 Gene (UniProtKB/Swiss-Prot). Protein Symbol:. Q6QNY1-BL1S2_HUMAN. Recommended name:. Biogenesis of ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=BLOC1S2
Clinical characteristics and pathophysiological mechanisms of focal and diffuse traumatic brain injury - Andriessen - 2010 -...
Lysosomal membrane rupture and cathepsin release play an important role in this process. Right upper corner: Increases in ... On the one hand, high cytosolic Ca2+ disrupts protein phosphorylation, microtubule construction and protease formation causing ... suggested that calpain activation is associated with lysosomal membrane disruption leading to leakage of hydrolytic lysosomal ... and the pro-apoptotic protein cytochrome-c, located between the inner and outer membranes of the mitochondria, are released ...http://onlinelibrary.wiley.com/doi/10.1111/j.1582-4934.2010.01164.x/full
8 Top Tips for Subcellular Fractionation | Proteintech Group
1], is an ideal method for looking at the activity of proteins at the membrane, especially if those proteins are recruited to ... activates protein kinase C, and increases cytosolic calcium in rat colonic epithelium. J. Clin. Invest. 85, 1296-303. ... A rapid method to separate endosomes from lysosomal contents using differential centrifugation and hypotonic lysis of lysosomes ... 1. Know your protein. Firstly, and most importantly, know your protein. This phrase is going to come up a lot, but don't worry ...https://ptglab.com/news/blog/8-top-tips-for-subcellular-fractionation/
Pancreatic Beta Cell Death: Novel Potential Mechanisms in Diabetes Therapy
... and clinical studies related to type 1 and type 2 diabetes. The journal welcomes submissions focusing on the epidemiology, ... Similarly, sphingosine (SPH), calpain, and hydroperoxidases induce permeation of the lysosomal membrane, causing the migration ... X-box binding protein 1; ERAD: endoplasmic reticulum-associated protein degradation; CHOP: CCAAT enhancer-binding protein ... Necrosome activation also favors ceramide production through the induction of the ceramidase enzyme, and it promotes cytosolic ...https://www.hindawi.com/journals/jdr/2018/9601801/
Human Metabolome Database: Showing metabocard for TG(16:1(9Z)/18:1(9Z)/20:4(5Z,8Z,11Z,14Z)) (HMDB0005441)
Phospholipase B1, membrane-associated. General function:. Involved in hydrolase activity. Specific function:. Membrane- ... Pancreatic lipase-related protein 3. General function:. Involved in catalytic activity. Specific function:. Not Available. Gene ... 3. Lysosomal acid lipase/cholesteryl ester hydrolase. General function:. Involved in lipid metabolic process. Specific function ... Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating ...http://www.hmdb.ca/metabolites/HMDB05441
Clathrin - the super cool cellular transport machine
Another protein that transports molecules across membranes in a similar way is caveolin. HybridAnimation's beautiful animation ... Their content is then digested by the lysosomal enzymes, the clathrin coat is dismantled and available for a new cycle. The ... As the triskelions accumulate, held together by AP2, they form a curved dimple (also known as a pit) on the cytosolic (or ... the process by which surface proteins are taken into the cell via a membrane-enclosed vesicle. For example, recent studies have ...http://betarhythm.blogspot.com/2006/03/clathrin-super-cool-cellular-transport.html