*  ISAC-NET - What is Cytometry?
Image cytometry is generally used for the multiparameter analysis of individual adherent cells, and is used to measure many of the same parameters as flow cytometry. Image cytometry, however, carries the added ability of three-dimensional imaging. 'High content analysis' is a term often applied to image cytometry in the context of high throughput screening, while 'tissue cytometry' refers to the application of these techniques to cells in situ. Image cytometry is generally performed using automated microscopy and computational image processing and analysis ...
  http://isac-net.org/About/What-is-Cytometry.aspx
*  An image cytometric technique is a concise method to detect adenoviruses and host cell proteins and to monitor the infection...
We investigated a possible image cytometric analysis of Ad early and late proteins together with cellular proteins. An image cytometry technique detected Ad proteins, E1A and hexon molecules, and cellular proteins, cleaved caspase-3, cyclin E and phosphorylated histone H3 molecules, at a single cell level. The technique is concise with less amounts of cells required in comparison with Western blot analysis and enables us to investigate molecular expression profiles quantitatively.. We detected E1A expression in cells infected with replication-competent Ad and compared detection sensitivity between image flow cytometer and Western blot analysis with the same Ab. We may not able to conclude differential sensitivity since a detection level is influenced by many factors including affinity of the second Ab used. Nevertheless, an image cytometry technique could detect a small fraction of Ad-infected population as being E1A-positive in contrast to Western blot analysis that detected the E1A level ...
  https://virologyj.biomedcentral.com/articles/10.1186/s12985-017-0888-0
*  Is there an advantage to be gained from adding digital image cytometry of brush cytology to a standard biopsy protocol in...
BACKGROUND AND STUDY AIMS: The current gold standard in Barrett's esophagus monitoring consists of four-quadrant biopsies every 1-2 cm in accordance with the Seattle protocol. Adding brush cytology processed by digital image cytometry (DICM) may furt
  http://www.biomedsearch.com/nih/there-advantage-to-be-gained/19418394.html
*  Patent US5250831 - DRAM device having a memory cell array of a divided bit line type - Google Patents
A memory cell array (50) of a DRAM has a so-called divided bit line structure including two regions (50a and 50) divided from each other. One bit line (24) of a bit line pair is connected to a predetermined memory cell in a first memory cell array block (50a) and is kept in unloaded state in a second memory cell array block (50b). The other bit line (25) of a bit line pair is kept in unloaded state in the first memory cell array block (50a) and is connected to a predetermined memory cell in a first memory cell array block (50b). In these structures, the load state is kept same in both bit lines of the bit line pair. In the memory cell array, four memory cells are disposed in a cross-relationship, and are connected to the bit line (24) through a contact portion (17) used in common by the four memory cells. The word lines (20a and 20b) are formed to obliquely cross the bit lines and to extend perpendicularly to each other. Capacitors (3) in the memory cells have portions extended over the word lines.
  http://www.google.com/patents/US5250831?dq=6008737
*  Patent US5856665 - Arc lamp stabilization and intensity control for imaging microscopy - Google Patents
An operator independent image cytometer having a method for image segmentation. Image segmentation comprises the steps of filtering a digital image of a cellular specimen and thresholding the resultant image. In addition, the thresholding may include the sorting of features extracted from the filtered image. The present invention also includes a method for cytometer autofocus that combines the benefits of sharpening and contrast metrics. The present invention further includes an arc lamp stabilization and intensity control system. The image cytometer has broad applications in determining DNA content and other cellular measurements on as many as 105 individual cells, including specimens of living cells. Image segmentation applications include PAP smear analysis and particle recognition.
  http://www.google.com/patents/US5856665?dq=6,250,774
*  Patent US5856665 - Arc lamp stabilization and intensity control for imaging microscopy - Google Patents
An operator independent image cytometer having a method for image segmentation. Image segmentation comprises the steps of filtering a digital image of a cellular specimen and thresholding the resultant image. In addition, the thresholding may include the sorting of features extracted from the filtered image. The present invention also includes a method for cytometer autofocus that combines the benefits of sharpening and contrast metrics. The present invention further includes an arc lamp stabilization and intensity control system. The image cytometer has broad applications in determining DNA content and other cellular measurements on as many as 105 individual cells, including specimens of living cells. Image segmentation applications include PAP smear analysis and particle recognition.
  http://www.google.com/patents/US5856665?dq=7,292,151
*  Video: Using Cellometer Image Cytometry for Cell Cycle Analysis
This focuses on using Propidium Iodide (PI) for cell cycle analysis. PI is a membrane exclusion dye and an intercalating agent that stains the cellular genome upon cell fixation.
  http://www.nexcelom.com/Support/video-presentation-cell-cycle-analysis.php
*  Plus it
As the features of the tumour raised the suspicion of squamous cell carcinoma, the patient was referred to the department of radiation oncology for radiation therapy. Histological examination of the tumour specimens revealed a papillary hyperplastic squamous epithelium without cell abnormalities. Ki-67 and p63 immunostaining showed a slightly increased proliferation rate in the middle layer of the epithelium. In situ hybridisation for the detection of HPV serotypes 1, 6, 7, 16, 18 and 31 showed evidence of previous HPV infection.. DNA image cytometry was performed in order to distinguish between hyperplastic and neoplastic lesions. Feulgen staining identified DNA contents in multiples of two in the euploid cells. In addition, stem lines at odd multiples (2.5c and 5c) could be found.. Knowing the HPV-RNA content of the tumour, it was decided not to perform radiation therapy. Further staging including 18-FDG (2-(18F)fluoro-2-deoxy-d-glucose) positron emission tomography-computed tomography (CT), ...
  http://erj.ersjournals.com/content/43/4/1198
*  Patent US6643196 - Redundant memory circuit for analog semiconductor memory - Google Patents
A redundant memory circuit for use in an analog semiconductor memory has a cell array divided into sectors. To replace bad sectors, the cell array may include a pair of redundant sectors disposed at opposite ends of the cell array, or may have a centrally located redundant sector. Alternatively, the redundant memory circuit may have a sector selection circuit that selects the sectors and redundant sector in sequence from one end of the cell array to the other, or may have a word line selector located in the center of the cell array, or may have two cell arrays and a redundant sector selection circuit that enables a bad sector in one cell array to be replaced by a redundant sector in either of the two cell arrays. These arrangements improve the capability for redundancy replacement and the quality of the reproduced analog signal.
  http://www.google.com/patents/US6643196?dq=7,546,338
*  Flow Cytometry / Cell Counting > Flow Cytometers (FACS) / Flow Cytometry Systems Laboratory Product Directory and Equipment...
Read reviews and compare manufacturers of Flow Cytometry / Cell Counting > Flow Cytometers (FACS) / Flow Cytometry Systems products in the SelectScience products and suppliers directory
  http://www.selectscience.net/flow-cytometry-+-cell-counting/product-directory/flow-cytometers-
*  HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers
DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresis-based protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidy-associated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n=19), diploid (n=31), and aneuploid (n=47) carcinomas. The results suggest that distinct protein ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:455499
*  Combining intensity, edge, and shape information for 2D and 3D segmentation of cell nuclei in tissue sections
Image analysis can provide genetic as well as protein level information from fluorescence stained fixed or living cells without loosing tissue morphology. Analysis of spatial, spectral, and temporal distribution of fluorescence can reveal important information on the single cell level. This is in contrast to most other methods for cell analysis, which do not account for inter-cellular variation. Flow cytometry enables single-cell analysis, but tissue morphology is lost in the process, and temporal events cannot be observed.. The need for reproducibility, speed and accuracy calls for computerized methods for cell image analysis, i.e., digital image cytometry, which is the topic of this thesis.. Algorithms for cell-based screening are presented and applied to evaluate the effect of insulin on translocation events in single cells. This type of algorithms could be the basis for high-throughput drug screening systems, and have been developed in close cooperation with biomedical industry.. Image based ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:163445
*  CUBE - High Performance Flow Cytometry System from Partec | SelectScience
Partec has launched a new instrument for flow cytometry. The CUBE 8 and the CUBE 6 are part of a uniquely designed high-performance flow cytometry instrument family. Designed to perfectly cover an extremly broad range of applications in healthcare (e.g. immunology), microbiology, industrial applications, agrosciences, aquaculture and breeding. The CyFlow® Cube offers up to 8 optical parameters and 6 colors with a wide choice of available laser light sources plus cell sorter option at an unmatche
  http://www.selectscience.net/product-news/partec-gmbh/cube---high-performance-flow-cytometry-system-from-partec/?artID=24082
*  Plus it
Purpose: Mantle cell lymphoma (MCL) is a mature B-cell lymphoma considered to be incurable with current treatments including: front-line rituximab in combination with multi-agent chemotherapy and for those eligible, high dose-chemotherapy and stem cell support or rituximab maintenance. On the other hand, achieving a complete remission by high-sensitive flow cytometry is associated with prolonged duration of remission, stressing the need to develop and/or incorporate novel agents into the management of MCL. To this end, we examined the activity of ofatumumab, an anti-CD20 monoclonal antibody with distinct binding and immunological properties than rituximab, in MCL pre-clinical models. Experimental Design: MCL cells were labeled with 51Cr prior to incubation with rituximab or ofatumumab (10 µg/ml) plus human serum or effector cells. 51Cr-release was measured and the percentage of lysis was calculated. Surface CD20, CD55 and CD59 were measured by Imagestream analysis. SCID mice inoculated ...
  http://clincancerres.aacrjournals.org/content/early/2015/05/09/1078-0432.CCR-15-0056
*  OSA | Exploiting chromatic aberration for image-based microscope autofocus
Autofocus is a critical aspect of any automated microscopy application. Here we report on the successful exploitation of chromatic aberration to speed autofocus for biological microscopy. Using pairs of lenses aligned nominally as infinite conjugates, the chromatic aberration produced by a microscope can be manipulated to achieve multiplanar imaging covering a wide range of spacings. Using both three-chip CCD and Bayer-array color cameras, chromatic aberration-based multiplanar imaging is used to reduce by a factor of 3 the number of mechanical movements of the biological sample necessary to determine best focus. Chromatic aberration-based autofocus is validated using a ...
  https://www.osapublishing.org/ao/abstract.cfm?URI=ao-50-25-4967
*  OSA | Exploiting chromatic aberration for image-based microscope autofocus
Autofocus is a critical aspect of any automated microscopy application. Here we report on the successful exploitation of chromatic aberration to speed autofocus for biological microscopy. Using pairs of lenses aligned nominally as infinite conjugates, the chromatic aberration produced by a microscope can be manipulated to achieve multiplanar imaging covering a wide range of spacings. Using both three-chip CCD and Bayer-array color cameras, chromatic aberration-based multiplanar imaging is used to reduce by a factor of 3 the number of mechanical movements of the biological sample necessary to determine best focus. Chromatic aberration-based autofocus is validated using a ...
  https://www.osapublishing.org/ao/abstract.cfm?uri=ao-50-25-4967&usertoken=%7Btoken%7D
*  Autofocus: phase detection
Inside the autofocus module (blue rectangle on the cross-section image above) there are two or more small image sensors, each one having a microlens above it. These sensors are typically one-dimensional, i.e. a row of pixels, and they come in pairs. Each pair constitutes one autofocus point (AF point), which looks at one part of the camera's field of view. A modern SLR may have many dozens of autofocus points, implemented as sensor tiles crammed onto a single chip. In the applet we show the sensors and microlenses of one AF point, which is looking at the center of our simulated camera's field of view. The blurry image at the bottom of the applet represents the image seen through this camera's viewfinder, and the tiny white rectangle represents the position of our AF point - at the image's center. If you follow the red bundle of rays in the applet, they start (at left) in the scene, pass through the top of the main lens, and are focused by Micro Lens 1 onto CCD 1. This CCD therefore sees a ...
  http://graphics.stanford.edu/courses/cs178-10/applets/autofocusPD.html
*  Cas9 and Guide RNA with DNA [image] | EurekAlert! Science News
Upon binding with guide RNA, the two structural lobes of Cas9 reorient so that the two nucleic acid binding clefts face each other, forming a central DNA binding channel that interfaces with target DNA.
  https://www.eurekalert.org/multimedia/pub/68343.php?from=259826
*  DNA histograms of vinorelbine and vinflunine in ECV304 | Open-i
DNA histograms of vinorelbine and vinflunine in ECV304 cells. detailed legend: DNA histograms of ECV304 cells treated with equitoxic concentrations of vinorelbi
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2311317_1471-2407-8-65-1&req=4
*  Patent US7542338 - Method for reading a multi-level passive element memory cell array - Google Patents
A four level passive element cell has memory states corresponding to decreasing resistance levels, which are preferably mapped respectively to data states 11, 01, 00, and 10 . The LSB and MSB are preferably mapped as part of different pages. To discriminate between memory cell states, the selected bit line current is sensed for at least two different combinations of reference current level and read bias voltage. A mid-level reference is used to read the LSB. When reading the MSB, a first reference between the 10 and 00 data states, and a second reference between 01 and 11 data states may be used, and the mid-level reference need not be used. In certain embodiments, the bit line current may be simultaneously compared against the first and second references, without requiring a delay to stabilize the bit line current to a different value, and the MSB generated accordingly.
  http://www.google.com/patents/US7542338?dq=7565338
*  Patent US5642312 - Flash EEPROM system cell array with more than two storage states per memory cell - Google Patents
A memory system made up of electrically programmable read only memory (EPROM) or flash electrically erasable and programmable read only memory (EEPROM) cells. An intelligent programming technique allows each memory cell to store more than the usual one bit of information. More than one bit is stored in a cell by establishing more than two distinct threshold states into which the cell is programmed. A series of pulses of increasing voltage is applied to each addressed memory cell during its programming, the state of the cell being read in between pulses. The pulses are terminated upon the addressed cell reaching its desired state or a preset maximum number of pulses has been reached. An intelligent erase algorithm prolongs the useful life of the memory cells. A series of pulses is also applied to a block of cells being erased, the state of at least a sample number of cells being read in between pulses. The erasing process is stopped when the cells being read are determined to have reached a fully erased
  http://www.google.ca/patents/US5642312
*  Patent US5812450 - Nonvolatile SRAM cells and cell arrays - Google Patents
A memory cell (400) for storing data on an integrated circuit. The memory cell (400) is static, nonvolatile, and reprogrammable. The layout of the memory cell is compact. In a first state, the logic output from this memory cell (400) is at about voltage level at a first conductor (505); and in a second state, the logic output is at about a voltage level at a second conductor (510). The memory cell (400) of the present invention includes a first programmable memory element (515) and a second programmable memory element (520). First programmable memory element (515) is coupled between the first conductor (505) and a sensing node (405). Second programmable memory element (520) is coupled between the sensing node (405) and the second conductor (510). In the first state, first programmable memory element (515) is not programmed, while the second programmable memory element (520) is programmed. In the second state, first programmable memory element (515) is programmed, while second programmable memory element
  http://www.google.com/patents/US5812450?dq=5,832,511
*  Cell Array manipulation (matlab) | Physics Forums - The Fusion of Science and Community
But I don't know how to concatenate them so that they are all one string. I think I am just supposed to use one command that can create the string ...
  https://www.physicsforums.com/threads/cell-array-manipulation-matlab.762731/
*  Nikon AF NIKKOR 50mm f/1.4D Autofocus Lens 1902 B&H Photo Video
Buy Nikon AF NIKKOR 50mm f/1.4D Autofocus Lens featuring F Mount Lens/FX Format, Aperture Range: f/1.4 to 16 Super Integrated Lens Coating. Review Nikon
  https://www.bhphotovideo.com/bnh/controller/home?O=NavBar&A=getItemDetail&Q=&sku=97413&is=GREY&si=rev
*  101 Dreams on the App Store
Read reviews, compare customer ratings, see screenshots and learn more about 101 Dreams. Download 101 Dreams and enjoy it on your iPhone, iPad and iPod touch.
  https://itunes.apple.com/my/app/101-dreams/id1052521636?mt=8