*  Plus it
... residues of complex multipass membrane proteins defined by the accessibility of covalent modification by sulfhydryl reagents to ... For competition analyses using MTS reagents (2 mm) or inhibitors (1 μm for L-685,458 and pep15, 10 μm for DAPT), cells were ... M4M (MTS-4-MTS) and M6M (MTS-6-MTS) are sulfhydryl-to-sulfhydryl cross-linking reagents with spacer moieties of 7.8 and 10.4 Å ... MTS reagents react specifically and rapidly with the sulfhydryl group of cysteine in an aqueous environment (Roberts et al., ...
  http://www.jneurosci.org/content/26/46/12081
*  Patent US5182107 - Transferrin receptor specific antibody-neuropharmaceutical or diagnostic ... - Google Patents
... a sulfhydryl group is introduced onto an amine group of one of the proteins using a compound such as Traut's reagent. The other ... In addition to covalent binding, conjugates can be formed employing non-covalent bonds, such as those formed with bifunctional ... Examples of peptides would be somatostatin analogues and enkephalinase inhibitors. Nucleotide analogs which can be used include ... contains the reagents and protocols for cleaving the antibody to obtain the fragments. The F(ab')2 fragment (0.2 mg doses) in ...
  http://www.google.com/patents/US5182107?dq=5,381,459
*  A novel bound form of plant invertase in rice suspension cells
Thiol reagents inhibited enzyme activity, but DTT restored it, suggesting the presence of sulfhydryl group (s) either in an ... The acid bound invertase (insoluble) with a basic pI is ionically or covalent bound to the cell wall. The acid unbound ... Fructose and glucose were inhibitors (Figure 8). Five mM of fructose or glucose was able to inhibit 50% of ITab activity. These ... Plant Materials and Reagents. Rice (Oryza sativa L. cv. Tainong 67) was grown in the field at the Experimental Farm of the ...
  http://ejournal.sinica.edu.tw/bbas/content/2002/2/bot432-04.html
*  Plus it
4b). This result suggested the AAEK class of inhibitors were mechanism-based inactivators, a type of covalent modifier that has ... The sulfhydryl of Cys184, presumably activated for nucleophilic attack via deprotonation by Arg197, forms a covalent bond with ... Although poor in inhibition, the usefulness of this reagent was realized when a kinetic analysis of the inhibitory pattern ... Low catalytic turnover, combined with long incubation times, probably introduces bias toward selection of covalent inhibitors. ...
  http://pharmrev.aspetjournals.org/content/60/1/128
*  Robert K. Crane - Wikipedia
"The inhibition of brain hexokinase by adenosinediphosphate and sulfhydryl reagents". Journal of Biological Chemistry 206, 1954 ... will] develop a chemical process to achieve synthesis of a new class of Sodium Glucose Cotransporters inhibitors (...), for the ... He demonstrated that neither the phosphorylation-dephosphorylation mechanism nor other covalent reactions accounted for glucose ... Furthermore, major pharmaceutical companies are developing inhibitors of the Na/glucose cotransporters to treat diabetes and ...
  https://en.wikipedia.org/wiki/Robert_K._Crane
*  Patent US4177253 - Magnetic particles for immunoassay - Google Patents
The active site can be protected during coupling reactions by employing a covalent blocking reagent, or by performing the ... and sulfhydryl; R is a member selected from the group consisting of lower alkoxy, phenoxy, and halo; and n is an integer having ... reaction in the presence of a substrate or specific inhibitors. Such protection techniques are known. Furthermore the coupling ... Immunological reagent and radioimmuno assay. US3977984 *. Oct 23, 1975. Aug 31, 1976. U.S. Philips Corporation. Chemically ...
  http://www.google.com/patents/US4177253?dq=%22Meaning-based+advertising+and+document+relevance+determination%22
*  Patent US7482173 - Method for attachment of silylated molecules to glass surfaces - Google Patents
Covalent attachment of specific binding members to a solid phase with two bifunctional reagents and a dithio compound. ... Suitable nucleophilic groups or moieties include hydroxyl, sulfhydryl, and amino groups or any moiety that is capable of ... and enzyme inhibitors and enzymes. Other specific binding members include, without limitation, DNA, RNA, polypeptide, antibody ... Reagent and method for attaching target molecules to a surface. US5868936. Jun 20, 1996. Feb 9, 1999. Baxter International Inc. ...
  http://www.google.com/patents/US7482173?dq=5,890,152
*  Patent US5066789 - Targeting substance-diagnostic/therapeutic agent conjugates having Schiff ... - Google Patents
... are treated with a reagent (for instance, iminothiolane) that adds free sulfhydryl groups to the targeting substance. The ... The Ar group of the linker may be derivatized with a Z substituent prior to covalent linkage of a targeting substance amine ... Ribosomal inactivating proteins (RIPs) are naturally occurring protein synthesis inhibitors that lack translocating and cell- ... Stable reagents for the preparation of radio pharmaceuticals. US6074659 *. Jul 10, 1998. Jun 13, 2000. Noerx Corporation. ...
  http://www.google.com/patents/US5066789?dq=5,832,511
*  Antinematodals Toxicity
Sulfhydryl reagents *React with molecules having -SH to form covalent bonds *Pyruvate dehydrogenase system especially sensitive ... The drug should not be used within a few days of use or exposure to cholinesterase inhibitors. An important source of exposure ... Signs of toxicity are typical of acetylcholinesterase inhibitors and include evidence of excessive acetylcholine at muscarinic ...
  http://www.cyto.purdue.edu/cdroms/cyto2/17/chmrx/nemathd2.htm
*  Patent WO1992021770A1 - Reagents and methods for performing two-step ion-capture binding assays - Google Patents
In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to ... The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/ ... without losing the capability to attract and retain the capture reagent. ... This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated ...
  http://www.google.ca/patents/WO1992021770A1?cl=en
*  6-Deoxyerythronolide B synthase - Wikipedia
The terminal sulfhydryl group of the phosphopantetheine is the site of attachment of the growing polyketide chain. Located at ... Functions via catalyzing acyl transfer from methylmalonyl-CoA to the ACP domain within the same module via a covalent acyl-AT ... is a much more practical method than synthesizing each product via chemical reactions in vitro due to the cost of reagents and ... length remains unknown but it is expected that ACP-bound fatty acid chains of the correct length act as allosteric inhibitors ...
  https://en.wikipedia.org/wiki/6-Deoxyerythronolide_B_synthase
*  US20080292618A1 - Intranuclear protein transduction through a nucleoside salvage pathway - Google Patents
Alternatively, molecules such asamino-sulfhydryl cross-linking reagents which are cleavable under mildly acidic conditions may ... Rapadocins, inhibitors of equilibrative nucleoside transporter 1 and uses thereof Also Published As. Publication number. ... Thus, the bond may be covalent or ionic. An example of a therapeutic complex where the linker is a chemical bond would be a ... Rapadocins, inhibitors of equilibrative nucleoside transporter 1 and uses thereof Families Citing this family (3). * Cited by ...
  https://patents.google.com/patent/US20080292618A1/en
*  US5719041A - DNA encoding ecotin homologs - Google Patents
TFPI inhibitors and methods of use WO2010071894A3 (en) * 2008-12-19. 2010-10-07. Baxter International Inc.. Tfpi inhibitors and ... The covalent attachment of polyethylene glycol (PEG) to ecotin and ecotin homologs may reduce the immunogenicity and toxicity, ... The sample (plasma and inhibitor) and reagent (CaCl2 /TF) were automatically mixed together after a 2 min incubation at 37° C. ... A table of protecting groups for amine, hydroxyl and sulfhydryl functions is provided on pages 149-151. ...
  https://patents.google.com/patent/US5719041?oq=patent%3A5992892
*  Лаборатория оксилипинов - ИБХ РАН
Gukovskaya A.S., Trepakova E.S., Zinchenko V.P., Korystov Y.N., Bezuglov V.V. (1992). Effect of the sulfhydryl reagent ... Markova L.N., Sakharova N.I.u., Bezuglov V.V. (2009). [The action of catecholamine-synthesis inhibitors and of spiperone on sea ... Methods of selective and nonselective covalent immobilization of genetically engineered proteins on molecules of natural ... The sulfhydryl reagent thimerosal at concentrations 5-100 microM has been found to induce a variety of changes in ion transport ...
  http://www.ibch.ru/structure/groups/oxylipin
*  Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, ... moiety and the antibody reagent are associated or linked to one another by direct chemical linkage such as involving covalent ... Sulfhydryl residues on β-galactosidase are exposed by reduction with dithiothreitol. β-galactosidase (30,000 units, grade VIII ... Coenzyme A disulfide reductase, and inhibitors thereof useful as antimicrobial agents. US6110676 *. 3 Nov 1997. 29 Aug 2000. ...
  http://www.google.ca/patents/US5200313
*  Synthesis and biochemical evaluation of benzyl propargyl ethers as inhibitors of 5-lipoxygenase
Keywords: Sulfolobus acidocaldarius, E. coli, RNase A, Archaeal ribosomes, Polyamines, Sulfhydryl reagents, Structural ... The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum ... Thermophilic ribosomes treated with destabilizing agents, such as sulfhydryl reagents [5,5 -Dithio-bis-(2-nitrobenzoic acid), N ... Keywords: Cyclooxygenase-2 inhibitors, 3-phenoxypyran-4-one derivatives, Quantitative structure-activity relationship. ...
  http://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb2k7/ijbb_feb07.asp
*  US Patent # 6,881,586. Retentate chromatography and protein chip arrays with applications in biology and medicine -...
d. Coordinate Covalent Interaction Adsorbents Adsorbents which are useful for observing the ability to form coordinate covalent ... transport inhibitors, surface-active agents (surfactants), mitochondrial and chloroplast function inhibitors, electron donors, ... Thus the functional group can be an inorganic oxide or a sulfhydryl group for gold. The other end of the linker generally has ... The introduction of eluant to small diameter spots of affinity reagent is best achieved by a microfluidics process. When the ...
  http://patents.com/us-6881586.html
*  Patent US5162504 - Monoclonal antibodies to a new antigenic marker in epithelial prostatic ... - Google Patents
Since non-covalent bonds are more likely to be broken before the antibody complex reaches the target site, covalent linkages ... Totally synthetic affinity reagents. US5747334 *. Jan 31, 1994. May 5, 1998. The University Of North Carolina At Chapel Hill. ... Inhibitors of NAALADase enzyme activity. US5824662 *. Sep 27, 1996. Oct 20, 1998. Guilford Pharmaceuticals Inc.. Treatment of ... Additionally, drugs with reactive sulfhydryl groups have been coupled to antibody molecules. ...
  http://www.google.com/patents/US5162504?dq=5,966,702
*  Application # 2005/0002865. Diagnostic/therapeutic agents - Patents.com
... may be considered as sulfhydryl reagents if linking occurs through the formation of disulphide bridges. Thus reagents which ... 0037] Linking of a reporter unit to the desired vectors may be achieved by covalent or non-covalent means, usually involving ... inhibitors inflammation platelet activating tumors, inhibitors of angiogenesis A factor antagonists inflammation platelet- ... Other useful carboxylic acid modifying reagents include isoxazolium derivatives such as Wo6dwards reagent K; chloroformates ...
  http://www.patents.com/us-20050002865.html
*  Patente US5486599 - Construction and use of synthetic constructs encoding syndecan - Google Patentes
Alternatively, a primary amine may be modified with Traut's Reagent to add a sulfhydryl (Blattler et al. (1985) Biochem 24:1517 ... The covalent linkage of a syndecan, or a portion thereof, with the heterologous molecule can be facilitated, in the instance ... Heparin-like molecules are also implicated in the biological activity of protease inhibitors like antithrombin III, heparin ... The first sulfhydryl reactive-protein prepared in the amine reaction step is mixed with the sulfhydryl-containing protein under ...
  http://www.google.es/patents/US5486599?dq=flatulence
*  US7407816B2 - Isoelectric particles and uses thereof - Google Patents
... of a covalent bond. As either the analyte or the reagent is charged, the formation or breaking of a covalent bond causes a ... A sulfhydryl group created after RAFT polymerization (Chiefari et al. Macromolecules 31:5559-5562, 1998) may be reacted, for ... enzymes and inhibitors, hybridizable nucleic acids, and the like. ... Covalent bonds are preferred. The member of the specific ... The member is linked to the particle through at least one covalent bond or non-covalent bond. ...
  https://patents.google.com/patent/US7407816
*  The Cytotoxicity of Benzaldehyde Nitrogen Mustard-2-Pyridine Carboxylic Acid Hydrazone Being Involved in Topoisomerase IIα...
It might be indicative that the BNMPH was not involved in iron deprivation from ribonucleotide reductase as those inhibitors ... Total RNA was extracted from the cells using Trizol reagent (Sangon, Shanghai, China) according to the manufacturer's protocol ... That was also indicative of covalent bond formed between stranded Ct-DNA. ... it has been well documented that nitrogen mustard reacts preferably with sulfhydryl compounds, so the BNMPH reactivity with ...
  https://www.hindawi.com/journals/bmri/2014/527042/
*  Patent US9272074 - Surgical fasteners and methods for sealing wounds - Google Patents
... covalent bonds are formed between the reactive members and the complementary reactive members, thus adhering the device to the ... covalent inhibitors and enzymatic transfers. For example, an azido group, N3, can be applied at the N-terminus of proteins or ... Sulfhydryl groups (e.g., as found on cysteine residues) may also be used as a reactive group for attaching PEG. In addition, ... 23, 2007). The critical reagent, a substituted cyclooctyne, possesses ring strain and electron-withdrawing fluorine ...
  http://www.google.ca/patents/US9272074
*  PEGylation by the Dock and Lock (DNL) Technique - Patent application
The PEG reagents are capped at one end with a methoxy group (m-PEG), can be linear or branched, and may contain one of the ... Under non-reducing conditions the EPO-DDD2 primarily resolves as a disulfide-linked covalent dimer (mediated by DDD2) with a Mr ... A 20 kDa PEG (starburst), AD2 peptide (helix), EDANS fluorescent tag (oval) and the positions of free sulfhydryl groups (SH) ... 2007; 336:215); small molecules such as inhibitors of integrin α4β1 (Pepinsky et al., J. Pharmacol. Exp. Ther. 2005; 312:742); ...
  http://www.patentsencyclopedia.com/app/20100261885
*  Glutathione reductase - Wikipedia
This anion then quickly breaks a disulfide bond of Cys58 - Cys63, forming a short lived covalent bond a stable charge-transfer ... There are two main classes of GR targeting compounds: Inhibitors of GSSG binding, or dimerization: Reactive electrophiles such ... Glutathione reductase (EC 1.8.1.7) catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione ( ... or the formed GSH can be visualized by Ellman's reagent. Alternatively the activity can be measured using roGFP (redox- ...
  https://en.wikipedia.org/wiki/Glutathione_reductase