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*  Expressed protein extraction from cos cells?
... Daniel Meier dmeier at post.its.mcw.edu Wed Jun 9 15:55:06 EST 1993 *Previous ... Hello All, What is the best way to extract expressed proteins from cos cells? Should one use a polytron? sonication? What ...
  http://www.bio.net/bionet/mm/methods/1993-June/005934.html
*  receptors on COS cell membrane
I need to know what kinds of membrane receptors are endogeneously @--, expressed on this cell line or COS-7 cells. @--, I would ... Along those lines, can anyone explain the difference between COS-1 and COS-7 cells? I'm especially interested in receptors and ... receptors on COS cell membrane. :-Peter obrien at pharm.med.upenn.edu Thu May 9 09:48:53 EST 1996 *Previous message: receptors ... I am planning to work on signal transduction with COS-1 cell line. @--, ...
  http://www.bio.net/bionet/mm/cellbiol/1996-May/004471.html
*  COS cells - Wikipedia
Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying ... COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a ... The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym "COS" is derived from the cells ... and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can ...
  https://en.wikipedia.org/wiki/COS_cells
*  cos-7 Cells | Thermo Fisher Scientific - CA
GIBCO brand cell line is a clonal isolate (COS-7L) adapted to serum-free monolayer culture.. ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ...
  https://www.thermofisher.com/ca/en/home/technical-resources/cell-lines/c/cell-lines-detail-5.html
*  Overexpression of endocytic proteins in COS cells
We have used 3 main methods to disrupt endocytosis in cells: use of inhibitory peptides, overexpression of protein domains and ...
  https://endocytosis.org/techniqs/COScells/COScells.html
*  Cloning of a novel surface antigen from the insect stages of Trypanosoma brucei by expression in COS cells. - MRC Weatherall...
In contrast the PSSA-2 antigen on COS cells was stained very weakly by whole parasite antisera. Northern blot hybridization ... Fluorescent antibody staining of PSSA-1 transfected COS cells with a panel of procyclin-specific monoclonal antibodies ... Furthermore, the antigen on COS cells was insensitive to treatment with phosphatidylinositol-specific phospholipase C ... stage surface antigen cDNAs by transient expression in mammalian COS-7 fibroblasts and 'panning' with a rabbit polyclonal ...
  https://www.imm.ox.ac.uk/publications/102482
*  In-Cell Western Used to Measure Response of COS-7 Cells to Hydroxyurea
... this plate-based immunofluorescent assay was used to measure the response of COS-7 cells to hydroxyurea. ... Detecting phospho-p53 in COS cells in response to Hydroxyurea. In this In-Cell Western assay application, the response of COS-7 ... In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea. June 15, 2012. Cancer Research, IRDye Reagents, ... Odyssey Imaging Systems, Proteomics Applicationscell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey ...
  http://www.licor.com/bio/blog/cancer-research/in-cell-western-assay-application-response-of-cos-7-cells-to-hydroxyurea/
*  Confocal images of COS-7 cells expressing mutant Tiam1 | Open-i
Confocal laser scanning microscopic images of COS-7 cells expressing C580 Tiam1 (A), MS-C580 T ... Confocal images of COS-7 cells expressing mutant Tiam1 proteins. ... Here we show that both in fibroblasts and COS cells, membrane ... Confocal images of COS-7 cells expressing mutant Tiam1 proteins. Confocal laser scanning microscopic images of COS-7 cells ... Confocal images of COS-7 cells expressing mutant Tiam1 proteins. Confocal laser scanning microscopic images of COS-7 cells ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2139766_JCB.michiels6m&req=4
*  The Takeaway From Penn Township For Cell Tower Cos.
... October 7, 2013 - Articles Law360 By Levin V. Czubaroff Share This Page ... Levin V. Czubaraoff's article "The Takeaway From Penn Township For Cell Tower Cos." was published in the October 7, 2013 issue ... As seen in a recent Pennsylvania Commonwealth Court case, it is imperative for cell tower companies to prepare their expert ... The zoning process for constructing new cellular telecommunications towers is very controversial, and often times, cell tower ...
  http://www.foxrothschild.com/publications/the-takeaway-from-penn-township-for-cell-tower-cos/
*  Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
Cells derived from the simian kidney, COS-7 cells, were transfected with a eucaryotic expression vector (pEUK-C1) containing ... Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.. Birnir, Bryndis Department of ... Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. Na(+)-dependent MeGlc ... We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:560734
*  Expression of the 7C8 antigen in COS cells. (A) Homog | Open-i
A) Homogenates of COS cells transfected with two of the pcDM8 expression plasmids isolated from immunoreactive ... Expression of the 7C8 antigen in COS cells. ( ... but untransfected COS cells (−) and cells transfected with ... Figure 4: Expression of the 7C8 antigen in COS cells. (A) Homogenates of COS cells transfected with two of the pcDM8 expression ... Figure 4: Expression of the 7C8 antigen in COS cells. (A) Homogenates of COS cells transfected with two of the pcDM8 expression ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2140167_JCB29357.f4&req=4
*  Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Transformed...
... cells displayed in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed ... View a smaller image of the African green monkey kidney (COS-7) cells. ... Transformed African Green Monkey Kidney Fibroblast Cells (COS-7). In contrast to quenching, photobleaching (also termed fading ... The culture of transformed African green monkey kidney (COS-7) cells displayed in the digital image presented above was ...
  http://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/cos7/cos7cellsexlarge11.html
*  Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Transformed...
... the mitochondria present in a culture of COS-7 kidney fibroblasts are easily observable due to staining with MitoTracker Red ... Transformed African Green Monkey Kidney Fibroblast Cells (COS-7). MitoTracker dyes are retained quite well after cell fixation ... View a larger image of the African green monkey kidney (COS-7) cells. ... The change in emission wavelength is useful in determining the ratio of active to non-active mitochondria in living cells. ...
  https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/cos7/cos7cellslarge12.html
*  Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Transformed...
The single cell featured in this section was resident in a COS-7 culture immunofluorescently labeled with primary anti-tubulin ... View a smaller image of the African green monkey kidney (COS-7) cells. ... Transformed African Green Monkey Kidney Fibroblast Cells (COS-7). The number of fluorescent probes currently available for ... The single cell featured in the digital image above was resident in a COS-7 culture immunofluorescently labeled with primary ...
  https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/cos7/cos7cellsexlarge8.html
*  Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...
COS-7 cells were used in this study as they are a well-established model cell line for gene transfer research. Both pDMAEMA and ... COS-7 cells were incubated with pDMAEMA- or PEI-based polyplexes for 60 minutes at 4°C. Subsequently, the cells were incubated ... Cell Culture. COS-7 African green monkey cells were grown in DMEM (Gibco BRL, Breda, The Netherlands) supplemented with ... COS-7 cells were incubated with polyplexes in the absence and presence of a variety of endocytosis inhibitors. Cells were pre- ...
  https://link.springer.com/article/10.1007%2Fs11095-007-9287-3
*  Plant Extracts of Winter Savory, Purple Coneflower, Buckwheat and Black Elder Activate PPAR-γ in COS-1 Cells but do not Lower...
Plant Extracts of Winter Savory, Purple Coneflower, Buckwheat and Black Elder Activate PPAR-γ in COS-1 Cells but do not Lower ... Although dose-dependent peroxisome proliferator-activated receptor-γ activity could be shown in COS-1 cells, the experiments in ... dose-dependently activate peroxisome proliferator-activated receptor-γ was determined in a reporter gene assay in COS-1 cells. ... mice lacked to confirm any anti-diabetic effect of the plant extracts in vivo and emphasizes the importance of verifying cell ...
  http://www.biomedsearch.com/nih/Plant-Extracts-Winter-Savory-Purple/23135898.html
*  Multipixel spectral imaging of green fluorescent protein (GFP) in COS-7 cells: folding kinetics and chromophore formation | ...
COS-7 kidney cells were transfected by a plasmid pEGFP-N1 plasmid followed by incubation for 15 hours for gen expression. At ... Multipixel spectral imaging of green fluorescent protein (GFP) in COS-7 cells: folding kinetics and chromophore formation ... Spectrally resolved imaging of Green fluorescent protein (GFP) expressed in living COS-7 kidney cells distinguished the ... different intervals the cells were examined by fluorescence microscopy and analyzed by a spectral imaging system. After 7 hours ...
  http://spie.org/Publications/Proceedings/Paper/10.1117/12.410631
*  Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter |...
Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter. ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ...
  http://www.biochemsoctrans.org/content/27/5/A149.3
*  Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C | Cell Physiology
Cell culture and transient transfection.. Monkey kidney COS-1 cells (from the European Collection of Cell Cultures) were grown ... Time course of human Na+-dependent vitamin C transporter hSVCT1 and hSVCT2 expression in COS-1 cells. A: COS-1 cells were ... Effect of PMA on the localization of hSVCT1 and hSVCT2 in COS-1 cells. COS-1 cells were transfected with either hSVCT1vh (A) or ... Expression and N-glycosylation of hSVCT1 and hSVCT2 in PMA-treated and nontreated transfected COS-1 cells. COS-1 cells were ...
  http://ajpcell.physiology.org/content/283/6/C1696
*  GenJet In Vitro DNA Transfection Reagent for COS Cells [SL100489-COS] - $156.00 : SignaGen Laboratories, A Gene Delivery...
Description GenJet DNA In Vitro Transfection Reagent for COS Cells is pre-optimized for COS cell transfection. COS is a cell ... GenJet In Vitro DNA Transfection Reagent for COS Cells [SL100489-COS] - ... It is also often used to transfect cells to produce recombinant proteins for molecular biology, biochemistry, and cell biology ... Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell line was obtained by immortalizing a cell line ...
  http://signagen.com/index.php?main_page=product_info&products_id=704
*  Hypoxanthine transport and transient expression of possible regulatory proteins in COS-1 cells - Kent Academic Repository
We have demonstrated that monkey kidney (COS-1) cells have a single type of nucleobase transport process. The system is sodium- ... Hypoxanthine transport and transient expression of possible regulatory proteins in COS-1 cells ... Transfecting COS-1 cells with opossum kidney cDNA fractions resulted in similar to 70% inhibition of hypoxanthine influx ... Hypoxanthine transport and transient expression of possible regulatory proteins in COS-1 cells. International Congress Series, ...
  https://kar.kent.ac.uk/16265/
*  Expression of Recombinant Alpha-1 Antitrypsin in CHO and COS-7 Cell Lines Using Lentiviral Vector
The results show that by employing a lentiviral vector, efficient genetic loading of CHO and COS-7 cells with the AAT gene was ... The transgene AAT-Jred chimer was transferred to CHO and COS-7 cell lines using this vector and its expressions were visualized ... a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT ... large amounts of recombinant human AAT protein were expressed in both CHO and COS-7 cell lines. This expression system ...
  http://www.ijbiotech.com/article_7070.html
*  Walter Rosenthal - NDI Foundation
He expects the cell culture model recently developed in his laboratory to be very useful in the examination of the action of ... Visualization of the V2 receptor expressed in COS cells -laser confocal microscopy- Dr. Rosenthal feels that the ability to ... COS cells DEFINITION: A monkey cell line that has been transformed by an SV40 viral genome containing a defective origin of ... cell culture DEFINITION: The maintenance or growth of an animal cell in vitro, or a culture of such cells. ...
  http://www.ndif.org/people/41-Walter_Rosenthal
*  Molecular Genetic Study of Finns With Hypoalphalipoproteinemia and Hyperalphalipoproteinemia | Arteriosclerosis, Thrombosis,...
Transient Transfection of COS Cells. Transfection of COS-7 cells (African green monkey kidney cells, ATCC CRL 1651) was ... LCAT α-activity in the serum or cell medium of the transfected COS cells was measured according to the method of Chen and ... Transient expression of the mutant LCATFin cDNA in COS cells disclosed markedly diminished LCAT enzyme activity. In the low-HDL ... The LCAT activity in media of the COS cells transfected with the pSVL SV40 vector alone was subtracted from the activities ...
  http://atvb.ahajournals.org/content/18/4/591
*  Apolipoprotein A-IFin | Arteriosclerosis, Thrombosis, and Vascular Biology
Expression of the Mutant Apo A-I cDNA in COS Cells. Wild-type and apo A-IFin-mutated apo A-I cDNAs were expressed in COS cells ... Transfection of the COS-7 (monkey kidney) cells was performed as described previously.26 In brief, subconfluent COS-7 cells ... Immunoprecipitation of apo A-I from the media of transfected COS cells. COS cells were transfected with wild-type or A-IFin- ... Lane 1: RNA of COS cells transfected with the expression vector pSVL SV40 alone; lane 2: RNA of the COS cells transfected with ...
  http://atvb.ahajournals.org/content/17/1/83