Expressed protein extraction from cos cells?
... Daniel Meier dmeier at post.its.mcw.edu Wed Jun 9 15:55:06 EST 1993 *Previous ... Hello All, What is the best way to extract expressed proteins from cos cells? Should one use a polytron? sonication? What ...http://www.bio.net/bionet/mm/methods/1993-June/005934.html
receptors on COS cell membrane
I need to know what kinds of membrane receptors are endogeneously @--, expressed on this cell line or COS-7 cells. @--, I would ... Along those lines, can anyone explain the difference between COS-1 and COS-7 cells? I'm especially interested in receptors and ... receptors on COS cell membrane. :-Peter obrien at pharm.med.upenn.edu Thu May 9 09:48:53 EST 1996 *Previous message: receptors ... I am planning to work on signal transduction with COS-1 cell line. @--, ...http://www.bio.net/bionet/mm/cellbiol/1996-May/004471.html
Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
Cells derived from the simian kidney, COS-7 cells, were transfected with a eucaryotic expression vector (pEUK-C1) containing ... Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.. Birnir, Bryndis Department of ... Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. Na(+)-dependent MeGlc ... We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic ...http://uu.diva-portal.org/smash/record.jsf?pid=diva2:560734
COS cells - Wikipedia
Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying ... COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a ... The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym "COS" is derived from the cells ... and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can ...https://en.wikipedia.org/wiki/COS_cells
Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...
COS-7 cells were used in this study as they are a well-established model cell line for gene transfer research. Both pDMAEMA and ... COS-7 cells were incubated with pDMAEMA- or PEI-based polyplexes for 60 minutes at 4°C. Subsequently, the cells were incubated ... Cell Culture. COS-7 African green monkey cells were grown in DMEM (Gibco BRL, Breda, The Netherlands) supplemented with ... COS-7 cells were incubated with polyplexes in the absence and presence of a variety of endocytosis inhibitors. Cells were pre- ...https://link.springer.com/article/10.1007%2Fs11095-007-9287-3
The Takeaway From Penn Township For Cell Tower Cos.
... October 7, 2013 - Articles Law360 By Levin V. Czubaroff Share This Page ... Levin V. Czubaraoff's article "The Takeaway From Penn Township For Cell Tower Cos." was published in the October 7, 2013 issue ... As seen in a recent Pennsylvania Commonwealth Court case, it is imperative for cell tower companies to prepare their expert ... The zoning process for constructing new cellular telecommunications towers is very controversial, and often times, cell tower ...http://www.foxrothschild.com/publications/the-takeaway-from-penn-township-for-cell-tower-cos/
Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter |...
Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter. ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ... Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-insensitive (ei) nucleoside transporter ...http://www.biochemsoctrans.org/content/27/5/A149.3
Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C | Cell Physiology
Cell culture and transient transfection.. Monkey kidney COS-1 cells (from the European Collection of Cell Cultures) were grown ... Time course of human Na+-dependent vitamin C transporter hSVCT1 and hSVCT2 expression in COS-1 cells. A: COS-1 cells were ... Effect of PMA on the localization of hSVCT1 and hSVCT2 in COS-1 cells. COS-1 cells were transfected with either hSVCT1vh (A) or ... Expression and N-glycosylation of hSVCT1 and hSVCT2 in PMA-treated and nontreated transfected COS-1 cells. COS-1 cells were ...http://ajpcell.physiology.org/content/283/6/C1696
Overexpression of endocytic proteins in COS cells
We have used 3 main methods to disrupt endocytosis in cells: use of inhibitory peptides, overexpression of protein domains and ...https://endocytosis.org/techniqs/COScells/COScells.html
Expression of the 7C8 antigen in COS cells. (A) Homog | Open-i
A) Homogenates of COS cells transfected with two of the pcDM8 expression plasmids isolated from immunoreactive ... Expression of the 7C8 antigen in COS cells. ( ... but untransfected COS cells (−) and cells transfected with ... Figure 4: Expression of the 7C8 antigen in COS cells. (A) Homogenates of COS cells transfected with two of the pcDM8 expression ... Figure 4: Expression of the 7C8 antigen in COS cells. (A) Homogenates of COS cells transfected with two of the pcDM8 expression ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2140167_JCB29357.f4&req=4
Pin1 Prolyl Isomerase Regulates Endothelial Nitric Oxide Synthase | Arteriosclerosis, Thrombosis, and Vascular Biology
Transfection of COS-7 Cells. COS-7 cells were transfected with various cDNA constructs cloned into the pcDNA3.1/V5-His A,B,C ... COS-7 cells were transfected with either WT eNOS or Ser116Asp with and without cotransfection with V5-tagged Pin1. A, cell ... COS-7 cells, which ordinarily express no endogenous eNOS, were transfected with either wild-type bovine eNOS (WT eNOS) or a ... Cell Culture. Primary cultures of bovine aortic endothelial cells (BAECs) were purchased from VEC Technologies Inc and were ...http://atvb.ahajournals.org/content/31/2/392
Cell Pic(s) of the Day - Cos' Blog
August 14, 2009 Cos General 0 I was going through my cell phone pics the other day and I realized that I have taken at least ... About Cos 5062 Articles Marcos Cosme has had a love for writing and film since he was little. By combing those two passions, he ... He is a Rabbit aficionado (he has a dwarf rabbit named Peanut-who is the logo of Cos' Blog) and is a Golden Retriever ...http://cosblog.cosmelentertainment.com/2009/08/14/cell-pics-of-the-day/
Walter Rosenthal - NDI Foundation
He expects the cell culture model recently developed in his laboratory to be very useful in the examination of the action of ... Visualization of the V2 receptor expressed in COS cells -laser confocal microscopy- Dr. Rosenthal feels that the ability to ... COS cells DEFINITION: A monkey cell line that has been transformed by an SV40 viral genome containing a defective origin of ... cell culture DEFINITION: The maintenance or growth of an animal cell in vitro, or a culture of such cells. ...http://www.ndif.org/people/41-Walter_Rosenthal
Molecular Genetic Study of Finns With Hypoalphalipoproteinemia and Hyperalphalipoproteinemia | Arteriosclerosis, Thrombosis,...
Transient Transfection of COS Cells. Transfection of COS-7 cells (African green monkey kidney cells, ATCC CRL 1651) was ... LCAT α-activity in the serum or cell medium of the transfected COS cells was measured according to the method of Chen and ... Transient expression of the mutant LCATFin cDNA in COS cells disclosed markedly diminished LCAT enzyme activity. In the low-HDL ... The LCAT activity in media of the COS cells transfected with the pSVL SV40 vector alone was subtracted from the activities ...http://atvb.ahajournals.org/content/18/4/591
Apolipoprotein A-IFin | Arteriosclerosis, Thrombosis, and Vascular Biology
Expression of the Mutant Apo A-I cDNA in COS Cells. Wild-type and apo A-IFin-mutated apo A-I cDNAs were expressed in COS cells ... Transfection of the COS-7 (monkey kidney) cells was performed as described previously.26 In brief, subconfluent COS-7 cells ... Immunoprecipitation of apo A-I from the media of transfected COS cells. COS cells were transfected with wild-type or A-IFin- ... Lane 1: RNA of COS cells transfected with the expression vector pSVL SV40 alone; lane 2: RNA of the COS cells transfected with ...http://atvb.ahajournals.org/content/17/1/83
Cell Cultures and Western Blotting.. HeLa cells and COS-1 cells were cultured as previously described (33, 34, 35) . Human lung ... Transfection of COS-1 Cells with the pSG5 Plasmid Encoding ICBP90.. COS-1 cells were transfected with pSG5 vector (Stratagene, ... Expression of ICBP90 and TopoIIα in COS-1 cells were analyzed by Western blot (A). Nontransfected COS-1 cells (Lanes 1 and 3) ... The 1RC1H-12 mAb was used for COS-1 cells instead of the 1RC1C-10 mAb because it did not label the endogenous ICBP90 of COS-1 ...http://cancerres.aacrjournals.org/content/60/1/121
Domain Mapping Studies Reveal That the M Domain of hsp90 Serves as a Molecular Scaffold to Regulate Akt-Dependent...
... and expression of eNOS in the cell lysate (bottom). B, COS cells were cotransfected with the cDNAs encoding V5-tagged eNOS ( ... COS-7 cells were plated in 60-mm dishes and transfected with eNOS (2 to 3 μg), HA-hsp90 (2 to 3 μg), and Flk (3 μg). To balance ... COS cells were transfected with the cDNAs for wild-type eNOS and flag-tagged hsp90 (Figure 2A) or V5-tagged eNOS and HA-tagged ... COS-7 cells were plated in 60-mm dishes and transfected with eNOS (2 to 3 μg), HA-hsp90 (2 to 3 μg), Flag-hsp90 constructs (3 ...http://circres.ahajournals.org/content/90/8/866
GFP-Shank forms filaments in COS cells. A-H, COS cells were transfected with the same constructs as those shown in Figure 1 and ... Only GFP-Shank1B, GKAP1A, and wild-type PSD-95 are distributed in clusters when coexpressed in COS cells. A, B, COS cells were ... GFPSh1BΔSH3 and GFPSh1BΔANK form filaments in COS cells only when coexpressed with full-length Shank1B. A, B, COS cells were ... GKAP and Homer1a interfere with Shank1B filament distribution in COS cells. A-F, COS cells were transfected with GFP-Shank1B, ...http://www.jneurosci.org/content/24/42/9391
Calabasas, CA). COS-7 cells were obtained from Dr. John Regan, University of Arizona. Expression vector pcDNA3 was obtained ... Transient Expression of PGT in HeLa Cells.. For HeLa cell expression, cells were grown to 80% confluence on 35-mm dishes and ... Transient Expression of Human FP Receptor in COS-7 Cells.. A cDNA sequence containing the entire coding region of the human FP ... COS-7 cells were transiently transfected using Lipofectin (Life Technologies, Inc., Gaithersburg, MD) according to ...http://molpharm.aspetjournals.org/content/58/6/1511
Cell-Based Assay with Intact COS-7 Cells Expressing Human AT1 Receptor.. Pretreatment of cells with AZL, olmesartan, or ... Cell Culture and Transfections.. COS-7 cells were maintained in 10-cm-diameter dishes at 37°C in a 5% CO2 atmosphere in ... Cell-Based Assay with Intact COS-7 Cells Expressing Human AT1 Receptors.. The expression vector pCAGGS was kindly donated by Dr ... COS-7 cells expressing human AT1 receptors were pretreated for 2 h with each compound and further incubated with AII (10 nM) ...http://jpet.aspetjournals.org/content/336/3/801
Detecting phospho-p53 in COS cells in response to Hydroxyurea. In this In-Cell Western assay application, the response of COS-7 ... cell proliferation assays, immunofluorescent assays, in cell westerns, in-cell ELISA, licor odyssey imagers, on-cell western ... In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea June 15, 2012. Cancer Research, IRDye Reagents, ... To others, it's a cell-based ELISA or an In-Cell ELISA (ICE Assay). To LI-COR, it's a In-Cell Western Assay (we call it an ICW ...http://www.licor.com/bio/blog/tag/protein-phosphorylation/
Patent US6139832 - Leukocyte adhesion inhibitor-1 (LAI-1) Polypeptides - Google Patents
... host cells and recombinant methods for producing the same. ... animal cells such as CHO, COS and Bowes melanoma cells; and ... The host cell can be a higher eukaryotic cell, such as a mammalian cell, or a lower eukaryotic cell, such as a yeast cell, or ... insect cells such as Drosophila and Sf9; animal cells such as CHO, COS or Bowes melanoma; plant cells, etc. The selection of an ... fungal cells, such as yeast cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; ...http://www.google.com/patents/US6139832?dq=6650327
Patent US6639052 - Human ADA2 polypeptides - Google Patents
... fungal cells, such as yeast; insect cells such as Drosophila S2 and Spodoptera Sf9; animal cells such as CHO, COS or Bowes ... The host cell can be a higher eukaryotic cell, such as a mammalian cell, or a lower eukaryotic cell, such as a yeast cell, or ... As representative examples of such cells, there may be mentioned endothelial cells, smooth muscle cells, embryonic kidney cells ... Cells are grown an extra 3 to 4 hours. Cells are then harvested by centrifugation. The cell pellet is solubilized in the ...http://www.google.com/patents/US6639052?dq=3657699
... and TSU human prostate cancer cells; 9 L rat gliosarcoma; C6 rat glioma cells; and Cos-7 green monkey renal epithelial cell ... Thereafter, 100-μl drops were transferred to Cos-7 cells cultured in 35-mm dishes. Three hours after transfection, cells were ... For counting apoptotic cells, cells were seeded on 96-well plates as described above. The cells were treated for various time ... Human cell lines including glioma cell lines (U87, U138, and U251) and prostate carcinoma cell lines (PC3, DU145, and TSU) were ...http://cancerres.aacrjournals.org/content/62/5/1433
Allows plasmid replication in mouse (WOP) and monkey CC (COS) cells. CC Medium is 1592 SM buffer. CC NM (lambda ZD35) CC CM (no ... CC A cell line suitable for transfection by released plasmid is Wgd5, CC ATCC CRL 1817. CC The plasmid insert can be released ... CC Allows expression of cloned cDNA in mammalian cells; can be used for CC transduction. ... CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phage) CC SP (ATCC) CC HO (bacteria-free lysate)(vertebrate cells)(E.coli) CC ...http://genome-www.stanford.edu/vectordb/vector_descrip/NOTCOMPL/LAMZD35.SEQ.html
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