Apoptosis in neural development and disease | ADC Fetal & Neonatal Edition
... control the decision to commit cell suicide, depending on whether pro- or anti-apoptotic partners predominate. In the later ... In this respect, targeted delivery of normal alleles to replace mutated genes or anti-apoptotic genes, to enhance survival of ... 1994) Overexpression of BCL-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia ... 1996) Integrated control of cell proliferation and apoptosis by oncogenes In: Thomas NSB, ed. Apoptosis and cell cycle control ...http://fn.bmj.com/content/77/3/F165
Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor...
... leading to cleavage of specific substrates involved in cell death. The activation of apoptotic genes will culminate in ... Bcl-2-Immunoglobulin transgenic mice demonstrate extended B cell survival and follicular lymphoproliferation. Cell, 57: 79-88 ... 1. Steller H (1995). Mechanisms and genes of cellular suicide. Science, 267: 1445-1449. [ Links ]. 2. Alnermi ES, Livingston DJ ... induces intracellular signals leading to a caspase cascade that will result in the transcription of genes controlling the cell ...http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004&lng=pt&nrm=iso&tlng=en
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 96, Anastasis, apoptosis, apoptotic bodies, caspase, cell death, cell shrinkage, cell suicide, ... Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell ... While IFN-dependent-innate and cell mediated immunity is important for eventual control of infection, innate immune cells, ... It effectively transduces naive mouse T cells if they express a transgenic Coxsackie adenovirus receptor (CAR). Importantly, ...https://www.jove.com/visualize/abstract/25993287/hepatitis-b-virus-x-protein-sensitizes-trail-induced-hepatocyte
Keystone Symposia | Scientific Conferences on Biomedical and Life Science Topics
Identification of Key Genes Controlling the Hypersensitive Cell Death through the Characterization of Two Arabidopsis Lesion ... The Characterisation of a Genetically Controlled Suicide Program in Response to Singlet Oxygen in Arabidopsis. ... Disease/Stress Protection in Plants Expressing Animal and Plant Anti-Apoptotic Genes. ... Transgenic Tobacco Plants Modified in SIPK Expression Display Differential Sensitivity to Abiotic Oxidative Stress and ...http://www.keystonesymposia.org/index.cfm?e=web.Meeting.Program&meetingid=658
Activation of an Endogenous Suicide Response after Perturbation of rRNA Synthesis Leads to Neurodegeneration in Mice | Journal...
Neural progenitors lacking TIF-IA undergo apoptotic cell death. A-F, Sagittal paraffin sections from control (A, C, E) and TIF- ... As the regulation of ribosome number is critical for cell growth and proliferation, rRNA genes (rDNA) are transcribed with high ... we have used a transgenic mouse line expressing the Cre recombinase only in neurons under control of the calcium/calmodulin- ... have shown that loss of TIF-IA blocks cell cycle progression and augments cell death. Importantly, cell cycle arrest and ...http://www.jneurosci.org/content/28/48/12759.full
... and various genes involved in the activation of apoptotic cell death.. In the two-strain method, the frequency of independent P ... In vitro-grown transgenic potato shoots expressing the ipt gene. The phenotype of a pSIM108 control plant (left) is compared to ... Rather than eliminating plants containing backbone, as is the case with the barnase suicide gene (Hanson et al., 1999), we ... C) PPO enzyme activity in field-grown wild type (WT), transgenic control (TC), and seven silenced lines. ...http://www.plantphysiol.org/content/135/1/421
Patent US7910370 - Methods of using BCL-2 for the therapeutic treatment and prevention of diseases - Google Patents
Methods to identify compounds that alter apoptotic cell death and to enhance monoclonal antibody production are also provided ... The invention provides a method of treating a disease or pathological condition resulting in apoptotic cell death. The method ... The physiological role of this self-induced suicide may be a negative feedback mechanism for controlling an immune response. ... with the idea of simultaneously providing genes for enhancement of cell survival (Bcl-2) and cell function (dopamine-β- ...http://www.google.com/patents/US7910370?dq=6650327
Programmed cell death - Wikipedia
... the developing nervous system by the targeted deletion of pro-apoptotic genes or by the overexpression of anti-apoptotic genes ... because mitochondria still retain their repertoire of molecules that can trigger cell suicide. It is not clear why apoptotic ... The ATG genes control the autophagosome formation through ATG12-ATG5 and LC3-II (ATG8-II) complexes. ATG12 is conjugated to ... Martinou, JC (1994). "Overexpression of BCL-2 in transgenic mice protects neurons from naturally occurring cell death and ...https://en.wikipedia.org/wiki/Programmed_cell_death
Frontiers | Elk-1 a Transcription Factor with Multiple Facets in the Brain | Neuroscience
Thus, Elk-1 plays a dual role in neuronal functions: pro-apoptotic within the cytoplasm, and pro-differentiation within the ... Thus, Elk-1 plays a dual role in neuronal functions: pro-apoptotic within the cytoplasm, and pro-differentiation within ... ... Endocytosis controls glutamate-induced nuclear accumulation of ERK. Mol. Cell. Neurosci. 41, 325-336. ... through its impact on genes involved in the core gene expression control. Interestingly, the srf gene itself has been ...https://www.frontiersin.org/articles/10.3389/fnins.2011.00035/full
Type I Interferons: Key Players in Normal Skin and Select Cutaneous Malignancies
showed that recombinant IFN-α at 1.98 × 102 U/mL resulted in 89% of control in cell number on day 5, compared to 78% at 2.18 × ... J. Rodriguez-Villaueva and T. J. McDonnell, "Induction of apoptotic cell death in non-melanoma skin cancer by interferon-α," ... Further, tissue samples from neither human nor transgenic mouse squamous cell carcinomas expressed significant levels of IFN-β ... This concomitant expression of CD95L and CD95 eventually led to cell death by suicide and fratricide, with the majority of ...https://www.hindawi.com/journals/drp/2014/847545/
Publications 2011 | Biocenter Oulu
J Mol Cell Cardiol 50:634-641, 2011.. Tavi P and Westerblad H. The role of in vivo Ca(2+) signals acting on Ca(2+) -calmodulin- ... Transgenic Res 21:163-176, 2011.. Sarajärvi T, Tuusa JT, Haapasalo A, Lackman JJ, Sormunen R, Helisalmi S, Roehr JT, Parrado AR ... Vuorela M, Pylkäs K, Winqvist R. Mutation screening of the RNF8, UBC13 and MMS2 genes in Northern Finnish breast cancer ... Secreted Wnt antagonist Dickkopf-1 controls kidney papilla development coordinated by Wnt-7b signalling. Dev Biol 353:50-60, ...http://www.oulu.fi/biocenter/publications-2011
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 96, Anastasis, apoptosis, apoptotic bodies, caspase, cell death, cell shrinkage, cell suicide, ... could be simply adapted for use with other transgenic animal models designed to assess the influence of particular genes on ... using antigen-negative cell lines as negative controls and antigen-positive cells as positive controls in the same mice for ... cell surface exposure of phosphatidylserine, and formation of apoptotic bodies. Anastasis can occur when apoptotic stimuli are ...https://www.jove.com/visualize/abstract/25811612/inhibition-of-type-vi-secretion-by-an-anti-tssm-llama-nanobody
Conditional Ablation of Cerebellar Astrocytes in Postnatal Transgenic Mice | Journal of Neuroscience
1995) Local control of granule cell generation by cerebellar Purkinje cells. Mol Cell Neurosci 6:230-251. ... 1992) P0 promoter directs expression of reporter and toxin genes to Schwann cells in transgenic mice. Neuron 8:507-520. ... 1993) Regression of established macroscopic liver metastases after in situ transduction of a suicide gene. Proc Natl Acad Sci ... An alternative but less favored mechanism for the bystander effect, phagocytosis of apoptotic bodies from the TK-expressing ...http://www.jneurosci.org/content/16/21/6908
MYC oncogene | ПРЕЦИЗИОННАЯ ОНКОЛОГИЯ
... and the human gene was later identified in the B-cell tumor Burkitt lymphoma. ... However, if the apoptotic pathway is affected by mutations, there will be an imbalance between proliferation and cell death ... This notion is supported by the finding that mnt-deficient mouse fibroblasts show a disrupted cell cycle control and can be ... The related MYCN and MYCL genes were found amplified in neuroblastoma and in small cell lung cancer, respectively. The myc ...http://cancerlink.ru/encyclopedia-cancer/m/myc-oncogene-en/
What's hot today: Current papers in developmental biology and gene function
Recent work indicates that Hox genes also control cell segregation and segmental boundary in various species, however the ... The cells that make up this epithelium are collectively eliminated through a highly synchronized form of apoptotic cell death, ... UAS transgenic flies were generated by identical chromosomal insertion, hence removing any transgenic position effects, and ... suicide-gene therapy, and cell-specific mRNA labeling techniques. Nevertheless, the enzymatic site of UPRT remains conserved ...http://www.sdbonline.org/sites/fly/aimain/model-organisms-august2015.htm
Clioquinol and pyrrolidine dithiocarbamate complex with copper to form proteasome inhibitors and apoptosis inducers in human...
Apoptotic cell death was measured by morphological changes, Hoechst staining, and poly(ADP-ribose) polymerase cleavage. When in ... and induce apoptotic cell death preferentially in breast cancer cells, less in premalignant breast cells, but are non-toxic to ... Breast cell lines, normal, immortalized MCF-10A, premalignant MCF10AT1K.cl2, and malignant MCF10DCIS.com and MDA-MB-231, were ... followed by measurement of proteasome inhibition and cell death. Inhibition of the proteasome was determined by levels of the ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1322
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 96, Anastasis, apoptosis, apoptotic bodies, caspase, cell death, cell shrinkage, cell suicide, ... For this, we accompany primary cell isolations of mouse VMs and/or AMs with critical quality control steps and direct membrane ... comparison of different cardiac or skeletal muscle cell types, phenotyping of transgenic models, and pharmacological or ... However, studies of selected sets of genes, such as sets of paralogs with a greater a priori chance of sharing a common ...https://www.jove.com/visualize/abstract/25962131/proflavine-hemisulfate-as-fluorescent-contrast-agent-for-point-care
Death on the slopes | EMBO Reports
Although we have long known about alternative forms of cell death, the mechanisms of these types of cell suicide are still ... apoptotic cell death, have the potential to influence cancer treatment. We can therefore proclaim that cell‐death research is ... Protein quality control-linking the unfolded protein response to disease Douglas M. Cyr, Daniel N. Hebert ... transgenic mice in which the immunoglobulin heavy chain enhancer (Eμ) drives the expression of c‐myc in cells of the B lineage ...http://embor.embopress.org/content/10/8/827
Ramasamy Paulmurugan | Stanford Medicine Profiles
PTS was found to induce apoptosis by altering the expression of apoptotic genes and the G2/M phase of cell cycle arrest. ... Seven swine received 23 intracardiac cell injections that contained control MSC and cell mixtures of MSC expressing a ... A transgenic tri-modality reporter mouse. PloS one Yan, X., Ray, P., Paulmurugan, R., Tong, R., Gong, Y., Sathirachinda, A., Wu ... In the present study, we evaluate the therapeutic potential of HSV1-sr39TK-NTR fusion dual suicide gene therapy system that we ...https://med.stanford.edu/profiles/ramasamy-paulmurugan
Patente US8093043 - β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing ... - Google Patentes
... to sensitize cancer cells to cell death or apoptosis. In certain embodiments, the invention relates to increasing BimEL levels ... Bcl-2-Interacting Mediator of cell death, Extra Long isoform) ... and Hybrid gb-Globin Genes in Transgenic Mice: Manipulation of ... show that SCFβTrCP- and Rsk-mediated degradation of BimEL controls the apoptotic response. (A) Mutation of Ser94/98 stabilizes ... Apoptosis is also called programmed cell death or cell suicide.. As used herein, apoptosis, cell suicide, and programmed cell ...http://www.google.es/patents/US8093043?hl=es&dq=flatulence
Cholesterol Homeostasis Imbalance and Brain Functioning: Neurological Disorders and Behavioral Consequences | Journal of...
Nat Cell Biol 3: 905-12.. 54 Sun Y, Yao J, Kim TW, Tall AR (2003) Expression of liver X receptor target genes decreases ... HTT seems to protect nerve cells from apoptotic events, thus exerting a crucial function in cell survival . Mutant HTT is ... Cholesterol content compared to controls. Species. Tissue. Reference. Alzheimer's disease. Increased. Rat. Brain (primary ... Alteration in cholesterol levels is often associated with increased inclination to commit suicide [104,108-110]. For instance, ...http://www.annexpublishers.co/full-text/JNND/101/Cholesterol-homeostasis-imbalance-and-brain-functioning-neurological-disorders-and-behavioral-consequences.php
Intramyocardial VEGF-B167 Gene Delivery Delays the Progression Towards Congestive Failure in Dogs With Pacing-Induced Dilated...
Kajstura J, Bolli R, Sonnenblick EH, Anversa P, Leri A. Cause of death: suicide. J Mol Cell Cardiol. 2006; 40: 425-437. ... in Ang II-treated cardiac myocytes than in untreated controls (note also the increased number of early apoptotic cells ... downregulates genes of the apoptosis/cell death-related pathways, in vitro, and can rescue neurons from apoptosis in mouse ... Presumably, an excessive VEGF-B167 overexpression would have caused marked hypertrophy, as described in transgenic mice.8 In ...http://circres.ahajournals.org/content/106/12/1893
CDKN2 and p53 genes cooperate to induce apoptotic tumor cell death. Nat. Med., 3: 313-319, 1997. ... into the tail vein in five daily doses of 108 pfu each starting either 3 or 5 weeks after tumor cell inoculations. Control mice ... Bao J. J., Zhang W. W., Kuo M. T. Adenoviral delivery of recombinant DNA into transgenic mice bearing hepatocellular carcinomas ... double suicide gene, and radiotherapy. Hum. Gene Ther., 9: 1323-1333, 1998. ...http://cancerres.aacrjournals.org/content/59/11/2623
Frontiers | Protecting and rescuing the effectors: roles of differentiation and survival in the control of memory T cell...
Development of CD8+ T cell memory is regulated by the transcriptional programs that control the differentiation and survival of ... Development of CD8+ T cell memory is regulated by the transcriptional programs that control the differentiation and survival of ... While the type of antigenic stimulation and level of inflammation control effector CD8+ T cell differentiation, availability of ... While the type of antigenic stimulation and level of inflammation control effector CD8+ T cell differentiation, availability of ...https://www.frontiersin.org/articles/10.3389/fimmu.2012.00404/full
Evidence that 4-Hydroxynonenal Mediates Oxidative Stress-Induced Neuronal Apoptosis | Journal of Neuroscience
Under control conditions ,5% of the cells were trypan blue-positive. Methods used to establish apoptotic cell death included ... 1995) Mechanisms and genes of cellular suicide. Science 267:1445-1449.. OpenUrlAbstract/FREE Full Text ... 1995) 3-Nitropropionic acid neurotoxicity is attenuated in copper/zinc superoxide dismutase transgenic mice. J Neurochem 65:919 ... Apoptotic cell death is characterized by cell shrinkage, cell surface blebbing, chromatin condensation, and DNA fragmentation ...http://www.jneurosci.org/content/17/13/5089.long