*  New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences ( One-column protein purification and n...)
Cloning PCR products using the LIC strategy is so efficient that as li...,New,Yeast,Cloning,System,for,Producing,Proteins,with, ... One-column protein purification and native eukaryotic amino acid se... figure 1 ...Stratagenes ESP LIC cloning and expression ... Cloning PCR products using the LIC strategy is so efficient that as little as 20 ng of PCR product is required for each cloning ... Enhanced PCR Cloning System. 7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning. 8. ...
  http://www.bio-medicine.org/biology-technology/New-Yeast-Cloning-System-for-Producing-Proteins-with-Native-Amino-Acid-0D-0A-0ASequences-44-1/
*  Creating empty vector by self-ligation - Molecular Cloning - BioForum
... posted in Molecular Cloning: I am attempting to create an empty vector from a commercial plasmid pCMVSport-6 (4.4kb) with Prp ... Why do you need to religate the vector, if you want to clone something else in sites which are present in the clone that you ... Why do you need to religate the vector, if you want to clone something else in sites which are present in the clone that you ... Why do you need to religate the vector, if you want to clone something else in sites which are present in the clone that you ...
  http://www.protocol-online.org/forums/topic/7379-creating-empty-vector-by-self-ligation/
*  Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor | JEM
The extent of each clone with reference to Fig. 1 is: clone 17, nucleotides 7-750; clone 2-1, nucleotides 1-750; clone 1A, ... Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor. John E. Sims, Douglas E. Williams, ... cDNA Cloning.. RNA was isolated from Z210R.1 cells, oligo-dT-primed cDNA was synthesized and cloned into the pDC406 vector, ... Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor ...
  http://jem.rupress.org/content/192/5/671
*  Cloning Mitochondrial Targeting Sequence - Molecular Cloning - BioForum
... posted in Molecular Cloning: Dear All,I'm actually having problems to clone a 95 bp fragment into a 7kbp vector.I ordered the ... Only one clone showed a band at approx. 100 bp. I sent this clone for sequencing. Surprisingly there was a 65 bp insert between ... I'm actually having problems to clone a 95 bp fragment into a 7kbp vector.. I ordered the dsDNA 95 bp oligo, containing a ... Cloning Mitochondrial Targeting Sequence. Started by speci, Apr 27 2013 11:34 AM ...
  http://www.protocol-online.org/forums/topic/29143-cloning-mitochondrial-targeting-sequence/
*  cloning vector,blunt zero cloning,pEASY®-Blunt Zero Cloning Kit,Cloning Vectors,Beijing TransGe
Cloning Vectors,Cloning and Mutagenesis System,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & ... Molecular cloning and characterization of the mitogen-activated protein kinase kinase gene (MKK4) and its promoter sequence ... Your present location:Home / Products / Cloning and Mutagenesis System / Cloning Vectors / pEASY®-Blunt Zero Cloning Kit ... pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells ...
  http://www.transbionovo.com/rx_detail/productId=208.html
*  PCR Cloning Kits (Lyophilized) - In-Fusion HD Plus EcoDry Cloning System
In-Fusion EcoDry PCR Cloning Kits allow you to clone any PCR fragment into any linearized vector in a single step without ... Molecular Weight Markers * DNA Molecular Weight Standard Markers * Protein Molecular Weight Markers ... Cloning Kits (Lyophilized)-In-Fusion HD Plus EcoDry Cloning System. In-Fusion HD EcoDry Cloning Plus kits are provided in a ... Cloning using modular parts *High-throughput cloning. Choose the Right In-Fusion Cloning Plus System for Your Needs. ...
  http://www.clontech.com/US/Products/Cloning_and_Competent_Cells/Cloning_Kits/In-Fusion_EcoDry?sitex=10020:22372:US&PROD=SX4GERv1jfVd88BfZpaLxznX:S&PROD_pses=ZGD8592936DEE3FF0854E97B023C9875F49E0E1B40CE2F631EBFD792A7CA7DE253AB4861DD081709006E74F7D22EBA4D3A81F98ED2B7958737
*  cloning trouble - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hello all, I'm quite new at cloning.. i'm using a kit from invitrogen. the pcr product i want to ... clone is 5 kb and i'm using chemically competent cells which i've stored in a -80 freezer. My first two trials yielded no ... Hello all, I'm quite new at cloning.. i'm using a kit from invitrogen. the pcr product i want to clone is 5 kb and i'm using ... Honestly, I am not very clear how are you trying to clone your insert, is it TA cloning? or which other kit are you using? ...
  http://www.protocol-online.org/forums/topic/6967-cloning-trouble/
*  A Modified MultiSite Gateway Cloning Strategy for Consolidation of Genes in Plants | SpringerLink
Molecular Biotechnology. February 2013. , Volume 53, Issue 2, pp 129-138 , Cite as ... 2000). GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods ... In this study, we modified gateway MultiSite cloning vectors and developed a hybrid cloning strategy which combines advantages ... Gene cloning vectors that facilitate the fusion, overexpression or down regulation of genes in plant cells are being used with ...
  https://link.springer.com/article/10.1007%2Fs12033-012-9499-6
*  Molecular Cloning - BioForum
Javascript Disabled Detected You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.. ...
  http://www.protocol-online.org/forums/videos/category-2-molecular-cloning/
*  cloning - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. cloning - (Jan/14/2008 ). I am trying to PCR the digested and dephosphorylated ...
  http://www.protocol-online.org/biology-forums/posts/33391.html
*  help - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. help - (Nov/26/2007 ). We've construct the cDNA library by using CloneMiner cDNA ...
  http://www.protocol-online.org/biology-forums/posts/32355.html
*  Cloning - Molecular Biology
Top : Forum Archives: : Molecular Biology. Cloning - RE digestion clean up (Jan/06/2006 ). Hi. Usually I lost much DNA when ...
  http://www.protocol-online.org/biology-forums/posts/12527.html
*  Transformation - Molecular Cloning - BioForum
... posted in Molecular Cloning: Hello I am using a geneart product (synthesized gene of my interest in a lenti back bone). They ... you should check the DNA they sent you for high molecular DNA that stays in the pockets on an agarose gel).. Another reason for ...
  http://www.protocol-online.org/forums/topic/23698-transformation/
*  re-digestion - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. re-digestion - (Mar/18/2010 ). Hi everyone. I am trying to do cloning. I ... I am trying to do cloning. I double digested my PCR products and vector (which had another insert). I have seen that this other ...
  http://www.protocol-online.org/biology-forums-2/posts/13791.html
*  KanR sequence - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. KanR sequence - (Mar/07/2012 ). What is the minimal cassette for ... I don't have other vectors for KanR... Well, I have one with AmpR, so I could clone the other way round (AmpR into KanR). But ... Depending on where you decided to clone, you may have omitted a 5' UP element from the sequence, 5' of the -35 region. In ... but it does not seem to confer KanR when I clone it into an AmpR plasmid. Is there something missing, or is it too big?. Any ...
  http://www.protocol-online.org/biology-forums-2/posts/24702.html
*  Cloning advice - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. Cloning advice - (May/21/2013 ). I clone a 915bp genes into PCDNA3. ... sometimes you lose things during cloning. typically there is something unanticipated like a recombination event or poor primer ... do you have other colonies you picked from the cloning to sequence? ...
  http://www.protocol-online.org/biology-forums-2/posts/29332.html
*  blunt-cloning - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. blunt-cloning - all are vector self-ligation products? (Jul/11/2003 ). Pages: ... I do blunt cloning directly from PCR products and don't really have problems. MMM...If CIP is not working very well maybe try ... I am doing a very tricky blunt-end cloning trying to get a 3 kb fragment into a 12 kb vector. Maybe the dephosphorylation makes ...
  http://www.protocol-online.org/biology-forums/posts/2540more1.html
*  ligation inhibitors - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. ligation inhibitors - (Mar/22/2006 ). I'm having problems with a ligation.. I've ... the DNA fragment which I'm cloning is a gene fusion with the GFP. Can the GFP (N-terminal fused) be toxic for the E .coli ...
  http://www.protocol-online.org/biology-forums/posts/14860.html
*  Transformation troubleshoot - Molecular Cloning
Top : Forum Archives: : Molecular Cloning. Transformation troubleshoot - (Jul/22/2008 ). Hi, I'm trying to isolate a plasmid ... I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it ...
  http://www.protocol-online.org/biology-forums/posts/38243.html
*  Restriction digest - Molecular Cloning
Top : New Forum Archives (2009-): : Molecular Cloning. Restriction digest - (Oct/22/2009 ). Hi, I am new to do research. I ... Is this plasmid DNA that has been cloned in E. coli? Genomic DNA or plasmids from other species can contain modifications that ...
  http://www.protocol-online.org/biology-forums-2/posts/11043.html
*  TA-Cloning - Molecular Biology
Top : Forum Archives: : Molecular Biology. TA-Cloning - molecular biology (Jul/20/2002 ). Which TA-Clonig Kit ist the best?. I ... I always use TA cloning kits from Invitrogen which is the company first sold TA kit. Quality is very stable, never failed. I ...
  http://www.protocol-online.org/biology-forums/posts/1085.html
*  Promoter Cloning - Molecular Biology
Top : Forum Archives: : Molecular Biology. Promoter Cloning - PCR amplification of a promoter and cloning (Mar/24/2005 ). I am ... It is simply a PCR cloning.. 3) Is it possible that even though I blasted the primers they are ampliflying other thing of the ... 2) Has anybody tried a similar cloning strategy? I guess yes, because the sequence is known, you don't need to do a genome walk ... 3) Is it possible that even though I blasted the primers they are ampliflying other thing of the same molecular weigh?. 4) The ...
  http://www.protocol-online.org/biology-forums/posts/5791.html
*  Restriction Endonucleases: Molecular Cloning and Beyond | NEB
The development of gene cloning vectors and selection methodologies enabled the cloning of REases. Cloning not only allowed the ... Restriction Endonucleases: Molecular Cloning and Beyond. Return to Restriction Endonucleases The sequence-specific DNA cleavage ... Home Products Restriction Endonuclease Products Restriction Endonucleases Restriction Endonucleases: Molecular Cloning and ... Restriction enzymes have been one of the major forces that enabled the cloning of genes and transformed molecular biology. ...
  https://www.neb.com/products/restriction-endonucleases/restriction-endonucleases/restriction-endonucleases-molecular-cloning-and-beyond
*  WikiGenes - Cloning, Molecular
Molecular cloning and characterization of an insulin-regulatable glucose transporter [29].. *Molecular cloning of cDNAs ... Disease relevance of Cloning, Molecular. *Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple ... High impact information on Cloning, Molecular. *Molecular cloning of NCR revealed novel members of the Ig superfamily ... Biological context of Cloning, Molecular. *From peptide sequence, molecular cloning revealed a cDNA encoding a novel protein, ...
  https://www.wikigenes.org/e/mesh/e/140.html