Effect of freezing on rabbit cultured chondrocytes
FILGUEIRAS, R.R et al. Effect of freezing on rabbit cultured chondrocytes. Arq. Bras. Med. Vet. Zootec. [online]. 2011, vol.63 ... Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic ... Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the ... Furthermore, the chondrocytes should be implanted after two weeks of culture, when the highest viability rate is found ...http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-09352011000100008&lng=en&nrm=iso&tlng=en
All Faculty | Duke Mechanical Engineering and Materials Science
... chondrocytes was significantly different at both time points (p,0.002). This finding suggests that chondrocytes from ... The objectives of this study were to characterize the mechanical behavior of primary human chondrocytes and to determine the ... A second goal was to quantify changes in the volume of isolated chondrocytes in response to mechanical deformation. The ... The results of this study indicate that the human chondrocyte behaves as a viscoelastic solid. No differences were found ...http://fds.duke.edu/db/pratt/mems/faculty/guilak/publications/64137
HKU Scholars Hub: Kinesin-1 is involved in chondrocytes adhesion to extracellular matrix and motility
Conference Paper: Kinesin-1 is involved in chondrocytes adhesion to extracellular matrix and motility. *Show simple item record ... Isolated mutant chondrocytes show reduced motility and adhesion ability to ECM proteins. Vinculin, the key regulator of focal ... Isolated mutant chondrocytes show reduced motility and adhesion ability to ECM proteins. Vinculin, the key regulator of focal ... To investigate kinesin‐1's role in chondrocytes intercalation, we generate kinesin‐1 heavy chain (Kif5b) knockout mouse. In the ...http://hub.hku.hk/handle/10722/189754
Genome editing of stem cells for cartilage repair and regeneration - The Kennedy Institute of Rheumatology
Lafont, J.E., Talma, S., Hopfgarten, C. & Murphy, C.L. Hypoxia promotes the differentiated human articular chondrocyte ... such as autologous chondrocyte implantation, where the cells could be edited in vitro before re-implantation back in the body. ... We will now use CRISPR-Cas9 to manipulate this pathway in both primary human chondrocytes and human chondrogenic stem cells, ... enhances matrix synthesis by human chondrocytes. Journal of Biological Chemistry. 2010; 285(27):20472-80. ...https://www.kennedy.ox.ac.uk/study-with-us/kennedy-trust-prize-studentships/projects/genome_editing_of_stem_cells_for_cartilage_repair_and_regeneration
Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta...
METHODS: We have recently demonstrated that in chondrocytes resveratrol modulates the NF-kappaB pathway by inhibiting the ... Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta- ... Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta- ... Anti-Inflammatory Agents, Apoptosis, Blotting, Western, Cell Proliferation, Cells, Cultured, Chondrocytes, Curcumin, Drug ...http://epubs.surrey.ac.uk/808686/
EPIGENETIC MODIFYING COMPOUNDS ALTER ACTIVITY OF PRIMARY HUMAN ARTICULAR CHONDROCYTES AND MESENCHYMAL STEM CELLS UNDERGOING...
EPIGENETIC MODIFYING COMPOUNDS ALTER ACTIVITY OF PRIMARY HUMAN ARTICULAR CHONDROCYTES AND MESENCHYMAL STEM CELLS UNDERGOING ... EPIGENETIC MODIFYING COMPOUNDS ALTER ACTIVITY OF PRIMARY HUMAN ARTICULAR CHONDROCYTES AND MESENCHYMAL STEM CELLS UNDERGOING ...https://oacentre.kennedy.ox.ac.uk/publications/466241
Evaluation of CD98 Expression in Normal and Osteoarthritic Human Articular Chondrocytes
Results: Human articular chondrocytes express CD98 and immunoreactivity revealed a similar heterogeneous pattern of CD98 in ... Chondrocytes enzymatically isolated from macroscopically normal and osteoarthritic (OA) articular cartilage were grown in short ... Although we detected no role for CD98 in chondrocyte mechanotransduction, it may be involved in other biological functions in ... To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechanotransduction. ...http://iji.sums.ac.ir/article_16986.html
The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli | Journal of...
The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli S. L. Bevill1, ... Ragan, P. M., Badger, A. M., Cook, M., Chin, V. I., Gowen, M., and Grodzinsky, A. J., 1999, "Down-Regulation of Chondrocyte ... The purpose of this study was to evaluate the response of chondrocytes to the effects of dynamic unconfined compression, TNF-α ... The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli. ASME. J ...http://biomechanical.asmedigitalcollection.asme.org/article.aspx?articleid=1886121&resultClick=1
Reactive oxygen species induce chondrocyte hypertrophy in endochondral ossification | JEM
Chondrocytes then proliferate rapidly to form a model for the bone. As chondrocytes divide, they form a columnar structure and ... Elevated ROS stimulate chondrocyte hypertrophy in vivo. To analyze the role of ROS in chondrocyte hypertrophy in vivo, we ... Elevated ROS stimulate chondrocyte hypertrophy in vitro. We next examined the effect of increased ROS on chondrocyte ... S1 shows the expression of nox1 in chondrocytes. Fig. S2 shows the Safranin-O staining of chondrocytes in mice treated with PBS ...http://jem.rupress.org/content/204/7/1613
Ca2+-sensitive phosphoinositide hydrolysis is activated in synovial cells but not in articular chondrocytes | Biochemical...
Ca2+-sensitive phosphoinositide hydrolysis is activated in synovial cells but not in articular chondrocytes. Ilaria CAPOZZI, ... In contrast, Ca2+ influx did not affect considerably the response of chondrocytes to ATP stimulation. In HIG-82 cells, the ... In HIG-82 synoviocytes, but not in chondrocytes, the Ca2+ ionophore ionomycin stimulated phosphoinositide hydrolysis in a ... We previously demonstrated that in cultured articular chondrocytes and HIG-82 synovial cells, studied with digitial ...http://www.biochemj.org/content/344/2/545
ZFIN Publication: Askary et al., 2015
Articular chondrocytes are distinct in producing lower levels of cartilage matrix and not being replaced by bone, yet how they ... Iroquois proteins may therefore have a conserved role in keeping chondrocytes in an immature state, with the lower levels of ... An early event in skeletal joint development is the specification of articular chondrocytes at the joint surface. ... Iroquois Proteins Promote Skeletal Joint Formation by Maintaining Chondrocytes in an Immature State ...http://zfin.org/ZDB-PUB-151112-6
Background Donor site morbidity, limited amounts of cells, lack of phenotype
Neocartilage was made by seeding 0.5 to at least one 1.0 X 106 nonexpanded chondrocytes per cm2 in HL-1 medium or a proprietary ... The techniques for chondrocyte isolation and neocartilage production were as referred to previously essentially.1 Briefly, ... Collagen type type and II IX mRNAs in clean juvenile chondrocytes had been 100- and 700-collapse higher, respectively, than in ... Strategies Chondrocyte Isolation and Neocartilage Creation Articular cartilage was from deceased human being donors following ...http://researchhunt.com/2017/09/22/background-donor-site-morbidity-limited-amounts-of-cells-lack-of-phenotype/
British Library EThOS: The effects of mechanical stimulation and cytokine stimulation on proteoglycan levels in human...
Transformed chondrocyte cell lines and primary OA human articular chondrocytes (HAC) were grown in monolayer culture. Cells ... Chondrocytes were also incubated with a cytokine cocktail containing IL-1β, TNFα, IL-6 and IFNγ (CYT). The transformed ... The effects of mechanical stimulation and cytokine stimulation on proteoglycan levels in human chondrocytes ... chondrocyte cell lines C20A4 and C2812 did not express detectable levels of NOS protein or nitrite activity. However increased ...http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.653045
Ultrasound stimulates proteoglycan synthesis in bovine primary chondrocytes.
However, chondrocytes from one donor cell isolation out of five remained non-responsive. Heat treatment alone did not increase ... Chondrocytes from five cellular isolations were exposed in triplicate to ultrasound (1 MHz, duty cycle 20%, pulse repetition ... Articular cartilage, chondrocyte, proteoglycan, ultrasound therapy National Category Cell and Molecular Biology Orthopedics ... This exposure profile was used in 10-minute-long heat treatments of chondrocytes. Heat shock protein 70 (Hsp70) levels after ...http://umu.diva-portal.org/smash/record.jsf?pid=diva2:843205
Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous...
... scaffold and monolayer cultures from ND chondrocytes; scaffold and monolayer cultures from OA chondrocytes; chondrocytes from ... Chondrocytes from these patients might thus slightly differ from chondrocytes obtained from healthy joints. . However, ACT ... In contrast, the expression of MMP1 was higher in OA chondrocytes cultured in ML compared with ND chondrocytes. The expression ... Thus, OA chondrocytes show a chondrogenic differentiation potential comparable with ND chondrocytes and the risk of ...https://arthritis-research.biomedcentral.com/articles/10.1186/ar2800
Indian hedgehog signals independently of PTHrP to promote chondrocyte hypertrophy | Development
... the pace of periarticular chondrocyte differentiation, the pace of chondrocyte hypertrophy and the rate of columnar chondrocyte ... Primary chondrocytes were transfected with the Topflash reporter plasmid using the Human Chondrocyte Nucleofector Kit (Amaxa), ... Chondrocyte hypertrophy is an essential process required for endochondral bone formation. Proper regulation of chondrocyte ... Chondrocytes proliferate and differentiate sequentially along the longitudinal axis. The length of the columnar chondrocyte ...http://dev.biologists.org/content/135/11/1947
Chondrocyte - Wikipedia
"Cartilage and Chondrocytes", in Gary Feinstein et al. Kelley and Firestein's Textbook of Rheumatology, 10th edition (2017) ... Chondrocytes (from Greek χόνδρος, chondros = cartilage + κύτος, kytos = cell) are the only cells found in healthy cartilage. ... The chondrocyte in cartilage matrix has rounded or polygonal structure. The exception occurs at tissue boundaries, for example ... Although the word chondroblast is commonly used to describe an immature chondrocyte, the term is imprecise, since the ...https://en.wikipedia.org/wiki/Chondrocyte
Human chondrocytes, reactive oxygen species production and Nox | SpringerLink
The cytochrome b558 content in chondrocytes was about 10 pmol/mg of proteins: this amount is lower than in neutrophils (200 ... The O2- produced by chondrocyte NADPH oxidase is about 0.1% of the respiratory burst measured in phagocytic cells such as ... The low level of superoxide anions O2- produced by chondrocytes in comparison with neutrophils may be involved in signal ... This work shows that three different human immortalized chondrocyte (HIC) cell lines (a gift from M Goldring) and native ...https://link.springer.com/article/10.1186/ar730
The ECM-Cell Interaction of Cartilage Extracellular Matrix on Chondrocytes
... and signaling proteins to maintain the chondrocyte phenotype, prevent chondrocyte apoptosis, and regulate chondrocyte-specific ... Chondrocytes express several members of the integrin family, including α5β1, the primary chondrocyte receptor for fibronectin. ... These chondrocyte integrins have a potential role in the initial adhesion and retention of chondrocytes at a cartilage defect ... TNF and IL-1 or anti-Fas antibody growth-regulated oncogene α in ECM can induce chondrocyte apoptosis. Chondrocyte apoptosis ...https://www.hindawi.com/journals/bmri/2014/648459/
Applications of Chondrocyte-Based Cartilage Engineering: An Overview
... Abdul-Rehman Phull,1 Seong-Hui Eo,1 Qamar Abbas,1 Madiha ... C. W. Archer and P. Francis-West, "The chondrocyte," International Journal of Biochemistry and Cell Biology, vol. 35, no. 4, pp ... M. Brittberg, "Autologous chondrocyte transplantation," Clinical Orthopaedics and Related Research, no. 367, pp. S147-S155, ... M. Brittberg, "Autologous chondrocyte implantation-technique and long-term follow-up," Injury, vol. 39, no. 1, pp. 40-49, 2008. ...https://www.hindawi.com/journals/bmri/2016/1879837/ref/
chick embryo chondrocytes Cells | Thermo Fisher Scientific - US
Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.. ...http://www.thermofisher.com/us/en/home/technical-resources/cell-lines/c/cell-lines-detail-188.html
Carticel (Autologous Cultured Chondrocytes for Implantation): Side Effects, Interactions, Warning, Dosage & Uses
Autologous Cultured Chondrocytes for Implantation) may treat, uses, dosage, side effects, drug interactions, warnings, patient ... Autologous cultured chondrocytes, the Carticel product, are derived from in vitro expansion of chondrocytes harvested from the ... Chondrocyte labeling in one of the rabbit studies8 and in an independent study in goats by Dell'Accio et al10 demonstrated that ... Bioactivity of autologous chondrocytes implanted under a periosteal patch was reported in the BLA for three rabbit studies6,7,8 ...https://www.rxlist.com/carticel-drug.htm
Chondrocyte | definition of chondrocyte by Medical dictionary
... chondrocyte explanation free. What is chondrocyte? Meaning of chondrocyte medical term. What does chondrocyte mean? ... Looking for online definition of chondrocyte in the Medical Dictionary? ... Related to chondrocyte: chondroblast, Eosinophils, Autologous Chondrocyte Implantation. chondrocyte. [kon´dro-sīt] one of the ... Chondrocyte , definition of chondrocyte by Medical dictionary https://medical-dictionary.thefreedictionary.com/chondrocyte ...http://medical-dictionary.thefreedictionary.com/chondrocyte