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*  Expression of the Endogenous 06-Methylguanine-DNA-methyltransferase Protects Chinese Hamster Ovary Cells from Spontaneous G:C...
... cell line that expressed 14,000 MGMT molecules/cell with those in the parental CHO cells lacking this DNA repair activity. The ... The frequency of G:C to T:A transversions was elevated in MGMT-proficient CHO cells. Expression of the enzyme considerably ... No significant difference from the parental CHO cells was found in the number or type of deletions, frame-shifts, multiple ... However, DNA sequence analysis of spontaneous mutations in the MGMT-proficient CHO cell line revealed a complex picture. ...
  http://cancerres.aacrjournals.org/content/52/23/6471
*  Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line
To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown ... In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High- ... Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line. Zhou, ... analysis was performed to monitor the status of the CHO-HBsAg aggregate structure assembly, particle size and molecular weight ...
  http://uu.diva-portal.org/smash/record.jsf?pid=diva2:104759
*  Sack: Freezing CHO cells - OpenWetWare
Gently resuspend cell pellet in 2 ml freezing medium*.. * Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, ... Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. ... Freezing CHO Cells. Christina McGee 4-5-2012. * Grow 10 cm dish ... Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO ( ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Sack:_Freezing_CHO_cells&oldid=667947" ...
  https://openwetware.org/wiki/Sack:_Freezing_CHO_cells
*  flp-in cho Cells | Thermo Fisher Scientific - CA
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... Developed by IVGN from CHO-K1 Chinese Hamster ovary (Kao and Puck, 1968) ATCC# CCL-61 also referred to as CRL-9618.. ... To give you confidence in the health of your cells every step of the way, ...
  https://www.thermofisher.com/ca/en/home/technical-resources/cell-lines/f/cell-lines-detail-538.html
*  CHO Cells
Stem Cell Products including NutriStem hPSC and MSC media, CryoStem Freezing Media, and other serum-free, xeno-free media ... Over 30 years of experience in developing and manufacturing a diverse cell culture media, including Sera, FBS, ... BIOGRO-CHO SFM Supplement (100X). For use with BIOCHO-1 SFM BASE, 5 mL. Learn More ... BIO-CHO-1 SFM Base Serum-free medium, 500 mL Learn More ... Stem Cell Research *Stem Cell Culture. *Stem Cell ...
  http://www.bioind.com/products/cell-culture/specialty-media/cho-cells/
*  Serum-free large-scale transient transfection of CHO cells
Keywords: Animals ; Bioreactors ; CHO Cells/*physiology ; Cell Culture Techniques/methods ; Cell Proliferation ; Cell Survival ... CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear ... Serum-free large-scale transient transfection of CHO cells. Derouazi, M.; Girard, P.; Van Tilborgh, F.; Iglesias, K.; Muller, N ... The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected ...
  https://infoscience.epfl.ch/record/105019
*  Adhering CHO cell culture - Tissue and Cell Culture - BioForum
... cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day- ... posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) ... Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i don't ... Have you done a viability assay to ensure that the floating cells are, in fact, dead?. CHO cells are damned hardy and will ...
  http://www.protocol-online.org/forums/topic/15050-adhering-cho-cell-culture/
*  Interaction between 2N-methyl-9-hydroxyellipticinium and ionizing radiation in CHO cells.
The main effect is observed in the case of exponentially growing cells which are also more sensitive to the drug alone than ... increases the killing of gamma-irradiated CHO cells. ... CHO Cells / radiation effects*. Cell Survival / radiation ... Previous Document: Targets for radiation-induced cell death: target replication during the cell cycle evaluated in cell.... ... increases the killing of gamma-irradiated CHO cells. The main effect is observed in the case of exponentially growing cells ...
  http://www.biomedsearch.com/nih/Interaction-between-2N-methyl-9/8103546.html
*  serumm free culture of adherent cho cells - Tissue and Cell Culture - BioForum
i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ... posted in Tissue and Cell Culture: Hi all, ... cho cells as that may change some properties of the cho cells. ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ...
  http://www.protocol-online.org/forums/topic/26144-serumm-free-culture-of-adherent-cho-cells/
*  Selection of chemically defined media for CHO cell fed-batch culture processes
... Pan, Xiao; Streefland, Mathieu; Dalm, Ciska; ... Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell ...
  https://brage.bibsys.no/xmlui/handle/11250/2433531
*  Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35 |...
... cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration ... Hiller GW, Ovalle AM, Gagnon MP, Curran ML, Wang WG (2017) Cell-controlled hybrid perfusion fed-batch CHO cell process provides ... CHO Site-specific integration CRISPR/Cas9 Cell line development C12orf35 Electronic supplementary material. The online version ... Li S, Gao X, Peng R, Zhang S, Fu W, Zou F (2016) FISH-based analysis of clonally derived CHO cell populations reveals high ...
  https://link.springer.com/article/10.1007%2Fs00253-018-9021-6
*  Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells
They also have the potential to be the best signal peptides for the production of new antibodies in CHO cells. ... develop a platform technology to optimize the signal peptides for high level production of therapeutic antibodies in CHO cells ... CHO cells Is the Subject Area "CHO cells" applicable to this article? Yes. No. ...
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116878
*  Cellvento CHO Cell Culture Media and Feeds </b> <...
With the introduction of Cellvento CHO media and Cellvento Feed products, MilliporeSigma offers you chemically defined, non- ... CHO Cell Culture Media and Feeds. Use and Application. CHO-S. Cell Lines. CHO-DHFR- and Slow Growing Cell Lines. CHO-K1. Cell ... CHO media and their companion feeds have been developed for use with CHO suspension cell types such as CHO-S, CHO-DHFR- and CHO ... Cellvento® CHO-210 Medium. Cellvento® CHO-220 Medium. Cellvento® CHO-200 Liquid Medium. Cellvento® CHO-210 Liquid Medium. ...
  http://www.emdmillipore.com/CA/en/products/biopharmaceutical-manufacturing/upstream-processing/cell-culture/cell-culture-media/cellvento-cho-cell-culture-media-and-feeds/NBCb.qB.Rq4AAAFAkf4.1Zwn,nav
*  Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells | Sigma-Aldrich
reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. ... The host cell line (CHOZN® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. Mild ... reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1 ... Overexpressing Mgat1 in wildtype CHO cells did not lead to increased sialylation ...
  https://www.sigmaaldrich.com/technical-documents/articles/biology/mgat1-disrupted-chinese-hamster-ovary-cells.html