Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana |...
Distribution of centromere histone H3 over the 180 bp clusters. The HTR12 protein is a centromere-specific histone H3 variant ... Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana ... Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana ... Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana ...http://jcs.biologists.org/content/117/14/2963.full
DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric...
Centromeric and pericentromeric regions are essential for chromosome condensation and the fidelity of segregation. Centromere ... we used a yeast two-hybrid screen and identified a novel interaction between DNMT3B and constitutive centromere protein CENP-C ... Centromeric and pericentromeric regions are essential for chromosome condensation and the fidelity of segregation.... ... we demonstrate for the first time that CENP-C recruits DNA methylation and DNMT3B to both centromeric and pericentromeric ...http://oxfordindex.oup.com/view/10.1093/hmg/ddp256
CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly | Journal of Cell Science
2013). A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C. Science 340, 1110-1113. doi: ... 1995). Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP ... 2012). CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat. Cell Biol. 14, 604-613. doi:10.1038/ ... 2004). Adaptive evolution of centromere proteins in plants and animals. J. Biol. 3, 18. doi:10.1186/jbiol11. ...http://jcs.biologists.org/content/128/24/4572
Overexpression of centromere protein K (CENPK) in ovarian cancer is correlated with poor patient survival and associated with...
In this study for the first time, we identified a member of the centromere protein (CENP) family, CENPK, which was specifically ... So far, cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) are the gold-standard tumor markers for ovarian cancer ... Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice.. Proceedings of the National ... Centromere protein-A, an essential centromere protein, is a prognostic marker for relapse in estrogen receptor-positive breast ...https://peerj.com/articles/1386/
Transcription-replication conflicts at chromosomal fragile sites-consequences in M phase and beyond | Springer for Research &...
Quenet D, Dalal Y (2014) A long non-coding RNA is required for targeting centromeric protein A to the human centromere. Elife 3 ... Du Y, Topp CN, Dawe RK (2010) DNA binding of centromere protein C (CENPC) is stabilized by single-stranded RNA. PLoS Genet 6: ... Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere. Genome Res 17:1146-1160 ... Blower MD (2016) Centromeric transcription regulates Aurora-B localization and activation. Cell RepGoogle Scholar ...https://rd.springer.com/article/10.1007/s00412-016-0617-2
KAKEN - Research Projects | Construction of mammalian artificial chromosomes using YAC (KAKENHI-PROJECT-08044200)
Publications] K.Yoda: 'Centromere protein B of African Green Monky cells : Gene structure,cellular expression and centromeric ... Publications] K.Yoda: 'Centromere protein B of African Green Monkey cells:Gene structure,cellular expression and centromeric ... Centromere protein B of African Green Monkey cells ; Genestructure, cellular expression and centromeric localization.' Mol.Cell ... Centromere is the chromosomal domain essential to the segregation of eukaryotic chromosomes. Although DNA is believed to be the ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044200/
Recombinant human KMT1B / SUV39H2 protein (ab80288)
SUV39H2 protein. Ab80288 is an active protein fragment produced in Escherichia coli and has been validated in FuncS… ... Chromosome , centromere. Associates with centromeric constitutive heterochromatin.. * Target information above from: UniProt ... Recombinant human KMT1B / SUV39H2 protein. See all KMT1B / SUV39H2 proteins and peptides. ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...http://www.abcam.com/recombinant-human-kmt1b-suv39h2-protein-ab80288.html
Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells | Journal of Cell Science
Deficiency of centromere-associated protein Slk19 causes premature nuclear migration and loss of centromeric elasticity. J. ... Bloom, G. S. and Endow, S. A. (1995). Motor proteins 1, kinesins. Protein Profile 2, 1105-1171. ... the inner centromere-like protein (INCENP, Sli15) and survivin (Bir1) (reviewed in Vader et al., 2006). This complex was shown ... Slk19p is a centromere protein that functions to stabilize mitotic spindles. J. Cell Biol. 146, 415-425. ...http://jcs.biologists.org/content/121/15/2529.full
Protocols and Video Articles Authored by Andreas Houben (Translated to German)
... centromeric loss of CENH3 protein rather than uniparental silencing of CENH3 genes causes centromere inactivity; (iii) in ... Loss of Centromeric Histone H3 (CENH3) from Centromeres Precedes Uniparental Chromosome Elimination in Interspecific Barley ... Tethering of cenH3 to non-centromeric loci led to de novo assembly of kinetochore proteins and to dicentric carrier chromosomes ... 2014 , Pubmed ID: 24887084 In eukaryotes, centromeres are the assembly sites for the kinetochore, a multi-protein complex to ...https://www.jove.com/author/Andreas_Houben?language=German
A novel DNMT3B splice variant expressed in tumor and pluripotent cells modulates genomic DNA methylation patterns and displays...
DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric ... while the PHD domain mediates protein-protein interactions and the ability of DNMT3B to repress transcription in a histone- ... Cancer cells express aberrant DNMT3B transcripts encoding truncated proteins. Oncogene. 2007;26:5553-5563. [PMC free article] [ ... Recombinant protein expression and purification. The DNMT3B3 and DNMT3B3Δ5 coding sequences were cloned into the pFastBacHT ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2783805/
Recombinant Human CENPO 293 Cell Lysate CENPO-7578HCL - Creative BioMart
CENPO; centromere protein O; CENP O; MGC11266; centromeric protein O; interphase centromere complex protein 36; CENP-O; MCM21R ... Products > Epigenetics Proteins > Cell Cycle Proteins > Cell division Proteins > Centromere Proteins Centromere Proteins NDE1 ... Antigen standard for centromere protein O (CENPO) is a lysate prepared from HEK293T cells transiently transfected with a ... Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for ...https://www.creativebiomart.net/description_409233_318.htm
Conservation of the Centromere/Kinetochore Protein ZW10 | JCB
The chromosomes of the budding yeast Saccharomyces cerevisiae have a well-defined point centromere: all centromeric functions ... Evolution of Centromere/Kinetochore Proteins. All centromeres must perform the similar functions of connecting chromosomes to ... that the MIF2 gene of Saccharomyces cerevisiaeencodes a centromere protein with homology to the mammalian centromere protein ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ...http://jcb.rupress.org/content/138/6/1289?ijkey=b8f73ff3f4cee63f88188497fbfbf3854da8faec&keytype2=tf_ipsecsha
Munira A. Basrai, Ph.D. | Principal Investigators | NIH Intramural Research Program
Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis. Mol Biol Cell. ... In addition to kinetochore proteins, association of cohesins with centromeres and along the length of the chromosomes ensures ... topology of centromeric chromatin, post-translational modifications of centromeric histones, and haploinsufficiency (HI) of the ... Cdc5 associates with centromeric chromatin to facilitate the removal of centromeric cohesins (Mishra et al., 2016). Future ...https://irp.nih.gov/pi/munira-basrai
Centromere Protein B (CENPB) ELISA Kits
This study shows that ICP0 induces the proteasomal-dependent degradation of the centromeric protein CENP-B in infected as well ... Protein level used designations for Centromere Protein B (CENPB) ELISA Kits CENP-B protein , centromere protein B , Major ... Additionally we are shipping Centromere Protein B Antibodies (41) and Centromere Protein B Proteins (7) and many more products ... Centromere Protein B (CENPB) ELISA Kits. CENPB product is a highly conserved protein that facilitates centromere formation. ...https://www.antibodies-online.com/abstract/Centromere+Protein+B+
Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein, Plant...
... thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein, Plant Molecular Biology" on ... Lineage-specific adaptive evolution of the centromeric protein CENH3 in diploid and allotetraploid Oryza species ... Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein. Moraes, Izabel ... The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold ...https://www.deepdyve.com/lp/springer_journal/recognition-of-a-thaliana-centromeres-by-heterologous-cenh3-requires-ULW0ei2LMB
Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant | Plant Cell
Centromere Divergence in Allotetraploids. The HTR12 protein from A. thaliana can be detected at the centromeres of all ... B) Anti-HTR12 antibody detection on a protein gel blot comparing bacterially expressed HTR12 protein with proteins detected in ... or centromeric H3s (CenH3s), which are thought to identify centromeres. The first recognized CenH3, the CENP-A protein ( ... Assuming a maximum of 10 centromeres or centromere pairs per nucleus, we found that in 6 nuclei the centromeres were ...http://www.plantcell.org/content/14/5/1053.long
SCC4 - MAU2 chromatid cohesion factor homolog - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - SCC4...
Binds to the nucleosome-free promoter regions of ribosomal protein genes and tRNA genes (PubMed:25173104). ... centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. P40090. C7GQ49. C8Z7G8. E7KBY1. N1P768. E7LTU4. ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. P40090. C7GQ49. C8Z7G8. E7KBY1. N1P768. E7LTU4. ...http://www.uniprot.org/uniprot/P40090
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Centromeric chromatin wraps around histones, providing an anchor for kinetochore proteins and spindle attachment. It is clear ... Recent reports extend our understanding of chaperones involved in centromere formation. And other accounts of half-sized, right ... Although direct explanations such as coupled transcription or direct protein-protein interactions are often considered, ... Here, we use queueing theory to investigate how waiting lines can lead to correlations between protein customers that are ...https://www.jove.com/visualize?author=Ruth%20Williams%20
Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes | SpringerLink
Kinetochore protein complexes (lilac rods) connect the centromere to the spindle microtubules (green rings) ... Blower MD, Sullivan BA, Karpen GH (2002) Conserved organization of centromeric chromatin in flies and humans. Dev Cell 2:319- ... Zinkowski RP, Meyne J, Brinkley BR (1991) The centromere-kinetochore complex: a repeat subunit model. J Cell Biol 113:1091-1110 ... 2011; Woodcock 1994), and even thinner helical protein assemblies such as bacterial type VI secretion systems (15 nm), ...https://link.springer.com/article/10.1007%2Fs00412-013-0423-z
Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta
... the establishment of a centromere must involve the deposition of the centromeric histone H3 variant designated CENH3. This ... The proteins interacting with plant centromere have also attracted attention [26-29]. The nucleosomes of centromeres are ... protein is generally viewed as the core of the centromeric complex linking centromeric DNA with the proteins of inner ... Modification of the proteins in the active kinetochore on B. vulgaris and PRO1 centromeres was demonstrated by immunostaining. ...https://www.hindawi.com/journals/ijpg/2009/721091/
CENPA Antibody (Monoclonal, H.577.2)
... centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; Histone H3-like centromeric protein A ... CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to ... Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. ... Synthetic peptide corresponding to the amino terminus of mouse CENP-A protein ...https://www.thermofisher.com/antibody/product/CENP-A-Antibody-H-577-2-Monoclonal/MA5-14829
Malik | The Company of Biologists
... at the essential centromeric DNA-protein interface. Much like host-pathogen interactions, this 'centromere drive' leads to ... surprisingly rapid evolution of existing centromeric protein-coding genes, invention of 'new' genes that rapidly become ...http://www.biologists.com/meetings/jeb2016/programme/malik/
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... additional proteins are assembled on centromeric chromatin. However, it remains unknown whether additional epigenetic features ... In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone CENP-A ... Late embryogenesis abundant (LEA) proteins are heat-soluble proteins and can prevent protein-aggregation in dehydrated ... SIRH12 encodes a protein highly homologous to the sushi-ichi retrotransposon Gag protein in the tammar wallaby, while SIRH12 in ...https://www.jove.com/visualize?author=Asao+Fujiyama
Chromosomes trapped in micronuclei are liable to segregation errors | Journal of Cell Science
... including centromeric proteins, all measurements were normalized to the average of 10 centromeres (CREST) in the primary ... Interestingly, we observed a ∼50% reduction of all tested protein levels at centromeres in micronuclei, irrespective of their ... 2016). CENP-A is dispensable for mitotic centromere function after initial centromere/kinetochore assembly. PubMed - NCBI. Cell ... 2007). Molecular mechanism of the nuclear protein import cycle. Nat. Rev. Mol. Cell Biol. 8, 195-208. doi:10.1038/nrm2114. ...http://jcs.biologists.org/content/131/13/jcs214742
Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein...
This block correlates with the viral immediate-early protein ICP0-induced degradation of the centromere protein CENP-C. However ... Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ... Here, we demonstrate that ICP0 also induces the proteasome-dependent degradation of the centromere protein CENP-A. By a series ... the observed pseudoprometaphase phenotype of infected mitotic cells suggests that the stability of other centromere proteins ...https://www.ncbi.nlm.nih.gov/pubmed/11053442