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*  Dental pulp stem cells - Wikipedia
Dental pulp stem cells (DPSCs) are stem cells present in the dental pulp, the soft living tissue within teeth. They are multipotent, so they have the potential to differentiate into a variety of cell types. Other sources of dental stem cells are the dental follicle and the developed periodontal ligament. A subpopulation of dental pulp stem cells has been described as human immature dental pulp stem cells (IDPSC). There are various studies where the importance of these cells and their regenerative capacity has been demonstrated. Through the addition of tissue-specific cytokines, differentiated cells were obtained in vitro from these cells, not only of mesenchymal linage but also of endodermal and ectodermal linage. Among them are the IPS, ...
  https://en.wikipedia.org/wiki/Dental_pulp_stem_cells
*  Midbrain Cues Dictate Differentiation of Human Dental Pulp Stem Cells Towards Functional Dopaminergic Neurons. - MU Digital...
Dental pulp originating from the neural crest is considered a better source of postnatal stem cells for cell-based therapies in neurodegenerative diseases. Dental Pulp Stem Cells (DPSCs) have been shown to differentiate into cell-types of cranial neural crest ontology; however, their ability to differentiate to functional neurons of the central nervous system remains to be studied. We hypothesized that midbrain cues might commit DPSCs to differentiate to functional dopaminergic cell-type. As expected, DPSCs in their naïve state spontaneously expressed early and mature neuronal markers like nestin, musashi12, β tubulin III and Map2ab. On exposure to midbrain cues (sonic hedgehog, fibroblast growth factor 8 and basic fibroblast growth factor), DPSCs showed upregulation of dopaminergic neuron-specific transcription factors Nuclear Receptor related protein 1 (Nurr1), Engrailed 1 (En1) and ...
  http://eprints.manipal.edu/139566/
*  Human adult dental pulp CD117/c-kit-positive networks of stromal cells
Autori: Rusu MC, Loreto C, Sava A, Manoiu VM, Didilescu AC. Editorial: Folia Morphol., 73(1), p.68-72, 2014.. Rezumat:. Dental pulp tissue was collected from 6 healthy adult patients, prior to prosthetic treatments, in order to evaluate the in situ phenotype of dental pulp stromal cells and compare with that of dental pulp stem cells. A CD34-/CD44+/CD105-/CD117+/CD146-/nestin- phenotype of stromal cells in the dental pulp core was found. Cells with a similar phenotype, but CD44-, were found in the cell rich zone. Dental pulp stromal networks (DPSNs) were found CD117+/CD44+ in the pulp core, but CD117+/CD44- in the cell rich zone. The c-kit-positive DPSNs were contacting pulp nerves and were, in this regard only, comparable ...
  http://ad-astra.ro/2014/02/12/human-adult-dental-pulp-cd117-c-kit-positive-networks-of-stromal-cells/
*  dental pulp stem cell transplants
The dental pulp stem cell industry is a rapidly evolving industry. Every month there are major events occurring in the sector that shift industry dynamics. Often, these events are announcements of technical or scientific advancements. Sometimes they are announcements of major industry alliances. Occasionally, they are announcements of a new industry competitor, a major milestone, or a significant funding award.. For those of us interested in the dental pulp stem cell industry, tracking these shifting industry dynamics is of paramount importance. For this reason, this post covers the most significant neural stem cell industry news events as of July 2015.. [Read more…] ...
  https://www.bioinformant.com/tag/dental-pulp-stem-cell-transplants/
*  Repositório Institucional UFC: Immune cell profile of dental pulp tissue treated with zoledronic acid
Abstract Immune cell profile of dental pulp tissue treated with zoledronic acid. International Endodontic Journal. Aim To characterize the pulp immune cell profile in the teeth of rats treated with zoledronic acid (ZA). Methodology Male Wistar rats (n = 6 per group) received four intravenous infusions of ZA at doses of 0.04, 0.20 or 1.00 mg kg 1 ZA or saline (control). On the 70th experimental day, they were euthanized. The first right molar was examined microscopically and submitted to toluidine blue reaction and immunohistochemical for CD68, tumour necrosis Factor (TNF)-a, interleukin (IL)-1b, inducible nitric oxide synthase (iNOS), nuclear factor kappa B (NF-kB) and IL-18 binding protein (IL-18 bp). The presence of ectasic/dilated vessels and inflammatory cells was analysed, and mast cells and mononuclear CD68-positive cells were counted along with the intensity of immunostaining (0-3) for inflammatory markers in odontoblasts and ...
  http://www.repositorio.ufc.br/handle/riufc/21485
*  original papers 英文原著論文 - Sawamoto Lab 澤本研究室
Kishimoto N, Asakawa K, Madelaine R, Blader P, Kawakami K, Sawamoto K (2013) Interhemispheric asymmetry of olfactory input-dependent neuronal specification in the adult brain. Nat Neurosci 16: 884-888. Pubmed. Yamagata M, Yamamoto A, Kako E, Kaneko N, Matsubara K, Sakai K, Sawamoto K, Ueda M (2013) Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice. Stroke 44: 551-554. Pubmed. 2012. Kato Y, Kaneko N, Sawada M, Ito K, Arakawa S, Murakami S, Sawamoto K (2012) A subtype-specific critical period for neurogenesis in the postnatal development of mouse olfactory glomeruli. PLoS ONE 7 (11): e48431. doi: 10.1371/journal.pone.0048431. FREE Full text Nakaguchi K, Jinnou H, Kaneko N, Sawada M, Hikita T, Saitoh S, Tabata Y, Sawamoto K (2012) Growth factors released from gelatin hydrogel microspheres increase new neurons in the adult mouse brain. Stem Cells Int 2012, Article ID 915160, 7 pages, doi: 10.1155/2012/915160. ...
  http://k-sawamoto.com/%E8%8B%B1%E6%96%87%E5%8E%9F%E8%91%97%E8%AB%96%E6%96%87/
*  JOMR | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells
The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.
  http://ejomr.org/JOMR/archives/2016/1/e4/v7n1e4ab.htm
*  JOMR | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells
The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.
  http://ejomr.org/JOMR/archives/2016/1/e4/v7n1e4ht.htm
*  Human adult dental pulp stem cells enhance poststroke functional recovery through non-neural replacement mechanisms. |...
Human adult dental pulp stem cells enhance poststroke functional recovery through non-neural replacement mechanisms. - Wai Khay Leong, Tanya L Henshall, Agnes Arthur, Karlea L Kremer, Martin D Lewis, Stephen C Helps, John Field, Monica A Hamilton-Bruce, Scott Warming, Jim Manavis, Robert Vink, Stan Gronthos, Simon A Koblar
  http://www.curehunter.com/public/pubmed23197777.do
*  Immortalization and characterization of human dental pulp cells with odontoblastic differentiation.
OBJECTIVE: To immortalize human dental pulp (HDP) cell showing stable growth and high mineralization activities in vitro. DESIGN: HDP cells were obtained from a healthy third molar and immortalized by transfection with human telomerase transcriptase
  http://www.biomedsearch.com/nih/Immortalization-characterization-human-dental-pulp/17350587.html
*  The formation of reparative dentine and H hl cells in the dental pulp
The odontoblasts of dental pulp tissue form primary and secondary dentine. If the pulp tissue is exposed, the odontoblast layer will be destroyed. The aim of direct pulp capping is to induce new hard tissue formation in order to keep the pulp tissue vital. If direct pulp capping is successful, tertiary dentine is formed. This hard tissue is denoted as reparative dentine and defined as a dentine matrix, which is formed by a new generation of odontoblast-like cells after an appropriate stimulus. Thus, reparative dentine is not formed by the original post-mitotic odontoblasts. Until recently it has been unclear which pulp cells differentiate into these odontoblast-like cells. Besides multipotent adult stem cells, fibroblasts and un-differentiated mesenchymal cells are mentioned in the literature as progenitor cells for ...
  http://www.quintpub.com/journals/endo/abstract.php?iss2_id=915&article_id=10469&article=2&title=The%20formation%20of%20reparative%20dentine%20and%20H%C3%B6hl%20cells%20in%20the%20dental%20pulp
*  Liver Cells Developed From Human Dental Pulp | TopNews United States
A group of Japanese researchers from the Nippon Dental University in Tokyo has conducted an experiment in which they have prepared liver cells from human dental pulp.. The research, which has been published in the Journal of Breath Research, has revealed that hydrogen sulphide (H2S), a major cause of bad breath, was the main component in transforming the cells into liver cells.. Lead author Ken Yaegaki said that they collected the pulp from the people who were coming in routine for dental checkups. He further affirmed that it is one of its kind research in which liver cells have been made from human dental pulp. The best part of the research is that the liver cells have been produced of great quality, which means there were fewer wrong cells.. "High purity means there are less 'wrong cells' that are being differentiated to ...
  http://topnews.us/content/246501-liver-cells-developed-human-dental-pulp
*  From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering | Evidence-Based Endodontics | Full Text
Despite tremendous efforts to develop stem cell-based tooth regenerative strategies, the identification of intrinsic properties of pulpal stem cells is an unresolved question. Dental pulp stem cells are heterogeneous cell subpopulations likely composed of stem cells at different differentiation stages along odontoblastic lineage, which has broken their characterization. Another major drawback in the dental field is that the physiological signals governing the recruitment of pulpal stem cells for tooth repair upon injury still remain unknown.. A decade ago, our laboratory established clonal odontogenic cell lines with stem cell properties from first molar tooth germs of day 18 mouse embryos transgenic for an adenovirus-SV40 recombinant plasmid (pK4) (Priam et al. 2005). These dental pulp-derived cell lines are stable and maintain an ...
  http://evidencebasedendodontics.springeropen.com/articles/10.1186/s41121-016-0002-x
*  From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering | Evidence-Based Endodontics | Full Text
Despite tremendous efforts to develop stem cell-based tooth regenerative strategies, the identification of intrinsic properties of pulpal stem cells is an unresolved question. Dental pulp stem cells are heterogeneous cell subpopulations likely composed of stem cells at different differentiation stages along odontoblastic lineage, which has broken their characterization. Another major drawback in the dental field is that the physiological signals governing the recruitment of pulpal stem cells for tooth repair upon injury still remain unknown.. A decade ago, our laboratory established clonal odontogenic cell lines with stem cell properties from first molar tooth germs of day 18 mouse embryos transgenic for an adenovirus-SV40 recombinant plasmid (pK4) (Priam et al. 2005). These dental pulp-derived cell lines are stable and maintain an ...
  https://evidencebasedendodontics.springeropen.com/articles/10.1186/s41121-016-0002-x
*  Quintessence Publishing
Objective: To investigate the expression of heat shock protein 70 in dental pulp tissue after cavity preparation. Methods: The expression of inductive heat shock protein 70 mRNA and hybridization allocation were detected in dental pulp tissues with deep cavity, shallow cavity, and no cavity. Results: The heat shock protein 70 mRNA expression in the shallow cavity and no cavity groups were negative, while those in the deep cavity group were positive with omnipresent staining on odontoblasts, fibroblasts, endothelial cells, and vascular smooth muscle cells. Conclusion: These results suggest that heat shock protein 70 may help to relieve dental pulp injury caused by exogenous factors and may maintain the normal function of dental pulp cells. The inductive heat shock protein 70 expression may have a threshold ...
  http://www.quintpub.com/journals/cjdr/archive_display_abstract.php3?journalArt=5332
*  Stimulatory effects of low-concentration reactive oxygen species on calcification ability of human dental pulp cells.
The present study was conducted to investigate the effects of reactive oxygen species (ROS) on the calcification ability of human dental pulp (HDP) cells. HDP cells were treated with 100 mumol/L hydrogen peroxide (H(2)O(2)) for 5 or 10 minutes (5-min
  http://www.biomedsearch.com/nih/Stimulatory-effects-low-concentration-reactive/19084128.html
*  Study shows how growth factor aids stem cells' ability to regenerate damaged teeth - Healthcanal.com : Healthcanal.com
Durham, NC - In a new study published in STEM CELLS Translational Medicine, researchers used a type of platelet-derived growth factor called PDGF-BB that enhances cells' ability to regenerate dentin-pulp complex.. Many in the medical community view stem cell therapy as a promising new strategy for repairing teeth once thought to be irreversibly damaged by tooth decay or dental injuries. The benefits of human dental pulp stem cells (hDPSCs), isolated from the living connective tissue in the tooth's center, on such damage have been well documented in studies.. However, previous studies also revealed several problems with this type of treatment, including limitations on how much mineralized tissue can be formed when scaffolds with hDPSCs alone were implanted in nude mice. More importantly, the narrow root canal of a tooth limits tissue infiltration and the revascularization process, which also worked against ...
  https://www.healthcanal.com/oral-dental-health/241465-study-shows-growth-factor-aids-stem-cells-ability-regenerate-damaged-teeth.html
*  Dental pulp stem cells transformed by 'bad breath' chemical
Japanese scientists have found that the odorous compound responsible for halitosis - otherwise known as bad breath - is ideal for harvesting stem cells taken from human dental pulp
  http://www.iop.org/news/12/feb/page_54038.html
*  British Library EThOS: An in vitro model for the study of transient receptor potential channels on human sensory neurons
Sensory neurons are responsible for detecting noxious chemical, mechanical and thermal stimuli and relaying the information to the central nervous system. They also contribute to local neurogenic inflammation via the release of neuropeptides. Sensory neurons express a variety of ion channels, including the transient receptor potential (TRP) channels. TRP channels are one of the main families of ion channels responsible for nociception and are desirable targets for analgesic and anti-inflammatory therapies. TRP channel studies on human sensory neurons has been hampered by the fact that peripheral neurons lack theis cell bodies, which are housed in neuronal ganglia and are inaccessible via peripheral tissue biopsy. To overcome this limitation we have differentiated human dental pulp stem cells towards a neuronal phenotype, termed peripheral neuronal equivalebts (PNEs), which have their cell bodies present. Microfluorimetric ...
  http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.680865
*  Dental Stem Cells Could Transform Cosmetic Dentistry And Overall Health - los coches dentistry
The potential from dental pulp stem cells include developing nerve and brain tissue, repairing muscle, developing bone, developing bone and tissue from the oral cavity, reconstructing affected cardiac tissue, developing cartilage, and treating type 1 diabetes to name a few. In the past, researchers harvested stem cells taken from bone marrow to create cells of various organs, however years of research implies that dental stem cells extracted from a tooth pulp might build organ tissues more quickly. Of major relevance is that UK scientists have ascertained that stem cells derived from tooth pulp effectively prevent a leading cause of blindness caused by traumatic injuries or degenerative diseases as reported in a July 2014 study on Neural Regeneration Research (Vol. 9, No. 6, 2014), University Of Birmingham, UK ...
  http://www.loscochesdentistry.com/dental-stem-cells-could-transform-cosmetic-dentistry-and-overall-health/
*  British Library EThOS: Early angiogenic change in dental pulp stromal cells cultured on biomimetic matrices
Revascularisation of the devitalised root canal is the Holy Grail of Endodontics and is being hotly pursued by many teams of clinicians but has yet to be achieved. The overall aim of this work was to attempt to induce early angiogenesis in human dental pulp stromal cells (DPSCS) in vitro and in vivo using a biomimetic approach based on combining scaffolds comprised of ECM components with DPSCs as a first step towards a tissue engineering strategy for dental pulp regeneration. After isolating DPSCs using collagenase digest, they were cultured on 1% hyaluronic acid (HyA) or Types I and III collagen matrices used either singly or in combination to determine the ability of these scaffolds to support/induce early angiogenic change in DPSCs both in vitro and in vivo. Angiogenic change was determined using a combined approach of DNA quantification, histology, immunohistochemistry to detect the angiogenic markers ...
  http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.689271
*  Dental Stem Cells in Research and Practice
The article focuses on the benefits and applications of dental stem cells for dental hygiene practice and research. It mentions various types of stem cells including embryonic and somatic stem cells, and discusses their advantages and disadvantages. It gives an overview of the history of the development of dental stem cells and their potential use in the treatment of diseases including Parkinson's. Also discussed is the future of dental stem cells research and dental hygienists' role in it. INSETS: Nondental Stem Cells for Dental Applications;How Stem Cells Are Extracted from Banked Tissue ...
  http://pdc-connection.ebscohost.com/c/articles/45634365/dental-stem-cells-research-practice
*  Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
  http://pubmedcentralcanada.ca/pmcc/articles/PMC5345141/
*  Therapeutic Potential of Dental Pulp Stem Cell Secretome for Alzheimer's Disease Treatment: An In Vitro Study
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4923581/