Toxins from cone snails: properties, applications and biotechnological production | SpringerLink
Recombinant peptide production can result in insoluble protein (inclusion bodies) or soluble protein. From inclusion bodies ... 2004). The use of highly soluble carrier proteins like, e.g., maltose-binding protein (Kapust and Waugh 1999), thioredoxin ( ... coli since they are either quickly degraded by cellular proteases or they accumulate to form insoluble aggregates, so-called ... Through affinity chromatography targeting such carrier proteins, these soluble fusion proteins can often be easily purified ( ...https://link.springer.com/article/10.1007%2Fs00253-008-1385-6
US8119117B2 - Adenoviral expression vector comprising a CD40L fusion protein adapted to elicit cellular immunity -...
... by administering an effective amount of the invention vector which enocodes the E6 or E7 protein of HPV. The immunity generated ... Replication-defective adenovirus human type 5 recombinant as a rabies vaccine carrier ... Recombinant adeno-associated virus expressing human papillomavirus type 16 E7 peptide DNA fused with heat shock protein DNA as ... Adenoviral expression vector comprising a CD40L fusion protein adapted to elicit cellular immunity Download PDF Info. ...https://patents.google.com/patent/US8119117?oq=6004266
HIV vaccine - Wikipedia
V520 contains a weakened adenovirus that serves as a carrier for three subtype B HIV genes (gag / pol / nef). Subtype B is the ... Biosantech has developed a novel vaccine called Tat Oyi, which aims at the tat protein. It is reported that the company's HIV ... Merck developed the experimental vaccine called V520 to stimulate HIV-specific cellular immunity, which prompts the body to ... The availability of several recombinant canarypox vectors has provided interesting results that may prove to be generalizable ...https://en.wikipedia.org/wiki/HIV_vaccine
KAKEN - Research Projects | The Analysis of Gene Expression of Morphogenetic Factors Implicated Development of Tooth Germ and...
1999, Research Category：Grant-in-Aid for Scientific Research (B), Section：一般, Research Field：Morphological basic dentistry ... Publications] Murata, M.: 'Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic ... Publications] Murata, M.: 'Bone augmentation by recombinant human BMP-2 and collagen on adult rat paricetal bone'Int. J. Oral ... Publications] M. Murata: 'Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470391/
PSYC Call for Commentators: BIOCHEMICAL BASIS OF COMA (969 lines)
There may also be different transporters in different cells, and the evidence suggests some type of carrier 'chaperone' protein ... Ferguson, S. S. & Caron, M. G. (1998) 'G protein-coupled receptor adaptation mechanisms.' Seminars in Cellular and ... Inhibitory effects of ketamine and halothane on recombinant potassium channels from mammalian brain.' Anesthesiology 84: 900- ... Kamata, H. & Hirata, H. (1998) 'Redox regulation of cellular signaling.' Cellular Signaling 11: 1-14. Katayama, Y., Mori, T., ...http://www.bio.net/bionet/mm/neur-sci/1999-September/040643.html
Geranyltranstransferase - Wikipedia
Ubiquinone is a key electron carrier in the electron transport chain of cellular respiration. Isoprenoids are a large group of ... A notable example of the former is the farnesylation of small G-proteins including Ras, CDC42, Rho, and Rac. The attachment of ... Tarshis LC, Yan M, Poulter CD, Sacchettini JC (Sep 1994). "Crystal structure of recombinant farnesyl diphosphate synthase at ... Ericsson J, Jackson SM, Edwards PA (Oct 1996). "Synergistic binding of sterol regulatory element-binding protein and NF-Y to ...https://en.wikipedia.org/wiki/Geranyltranstransferase
Pharmaceutics | Free Full-Text | From Molecular to Nanotechnology Strategies for Delivery of Neurotrophins: Emphasis on Brain...
Polymer-based carriers have provided sustained neurotrophin delivery, whereas lipid-based particles have contributed also to ... Recent developments in nanoscale carriers for encapsulation and transport of BDNF are highlighted. ... I. Administration of recombinant proteins by direct injection. Subcutaneous and intrathecal injection BDNF Amyotrophic lateral ... cellular model of neurodegeneration. in vitro. increased cell survival. . Triglyceride matrix implants. BDNF (lysozyme ...http://www.mdpi.com/1999-4923/5/1/127/htm
Rinderpest - microbewiki
Raha, T.; Pudi, R.; Das, S.; Shaila, M.S.; "Leader RNA of Rinderpest Virus Binds Specifically With Cellular La Protein: A ... RPV has no carrier state (Shaila, 2006). Although it formerly occurred worldwide (save Australia), the vast majority of ... "Expression in Cattle of Epitopes of a Heterologous Virus Using a Recombinant Rinderpest Virus." Journal of General Virology 80 ... This contains six genes that code for structural proteins, and two that code for non-structural proteins (Shaila, 2006) that ...https://microbewiki.kenyon.edu/index.php/Rinderpest
Non-toxic mucosal adjuvant - Patent # 7485304 - PatentGenius
Sanchez, et al., "Recombinant Cholera Toxin B Subunit and Gene Fusion Proteins Oral Vaccination," Res. Microbiol. 141:971-979 ( ... McKenzie, et al., "Cholera Toxin B Subunit as a Carrier Protein to Stimulate a Mucosal Immune Response," J. Immunol. 133(4): ... Pierce, et al., "Cellular Kinetics of the Intestinal Immune Response to Cholera Toxoid in Rats," J. Exp. Med. 142:1550-1563 ( ... Suitable carriers are typically large, slowly metabolized macromolecules such as proteins, polysaccharides, polylactic acids, ...http://www.patentgenius.com/patent/7485304.html
Polymeric carriers of therapeutic agents and recognition moieties for antibody-based targeting of disease sites - Immunomedics,...
... of cAMP-dependent protein kinase to microtubule-associated and other cellular proteins", Proc. Natl. Acad. Sci. USA 81:6723-27 ... A chimeric antibody is a recombinant protein in which the variable regions of a human antibody have been replaced by the ... Taylor, S., "cAMP-dependent Protein Kinase", J. Biol. Chem. 1989;264(15):8443-8446. Uekama et al., "Cyclodextrin Drug Carrier ... Carr et al., "Identification of Sperm-specific Proteins That Interact with A-kinase Anchoring Proteins in a Manner Similar to ...http://www.freepatentsonline.com/8889835.html
Immunofluorescence Protocol | Immunohistochemistry Protocol | Immunofluorescence Techniques| immunofluorescence staining...
... recombinant proteins, or synthetic peptides coupled to a carrier protein. Optimal detection depends on the quality of the ... Interactions between cellular components with dimensions below the diffraction-limited resolution of the light microscope can ... The calcium-binding protein parvalbumin (red) was detected in a neuron of rat cerebral cortex with monoclonal antibody followed ... Its main application is the detection of proteins and other biomolecules in cells and tissues. Closely related techniques ...http://www.academicroom.com/topics/immunofluorescence-protocol
MRN, CtIP, and BRCA1 mediate repair of topoisomerase II-DNA adducts | JCB
Antibodies, immunodepletions, and recombinant proteins. The following antibodies were used in these studies: mouse anti-CtIP ( ... Studies in mammalian cells have furthered our understanding of the cellular strategies used to deal with these adducts. Here, ... and its ablation could contribute to the cancer susceptibility of BRCA1-mutation carriers. ... S2 describes the assay to detect etoposide-induced Top2-DNA adducts in egg extracts and purified recombinant CtIP proteins used ...http://jcb.rupress.org/content/212/4/399?cct=1418
Biology | Free Full-Text | Transcriptional Regulation of the Mitochondrial Citrate and Carnitine/Acylcarnitine Transporters:...
... the citrate carrier (CIC) and the carnitine/ acylcarnitine/carrier (CAC), which are members of the mitochondrial carrier gene ... but also many metabolic pathways and cellular functions at the molecular level. The regulation of the expression of many genes ... Heterologous expression in yeast and Escherichia coli, site-directed mutagenesis, reconstitution of the recombinant proteins in ... Metabolic pathways are often located in different cellular compartments and need to be linked through translocation of solutes ...http://www.mdpi.com/2079-7737/2/1/284/htm
Frontiers | Development and Applications of VSV Vectors Based on Cell Tropism | Microbiology
... gene has been used to produce a pseudotype or recombinant VSV possessing the envelope proteins of heterologous viruses. These ... A recombinant VSV lacking its own envelope (G) gene has been used to produce a pseudotype or recombinant VSV possessing the ... Here we describe procedures for producing pseudotype or recombinant VSVs and a few of the more prominent examples from among ... Here we describe procedures for producing pseudotype or recombinant VSVs and a few of the more prominent examples from among ...https://www.frontiersin.org/articles/10.3389/fmicb.2011.00272/full
Ltd., Tokyo, Japan). Cellular protein content was measured by the method reported previously (Bradford, 1976) using bovine ... In our previous study, we constructed a recombinant adenovirus containing human PEPT1 and enhanced yellow fluorescent protein ( ... 1997) Carrier-mediated transport of oligopeptides in the human fibrosarcoma cell line HT1080. Cancer Res 57:4118-4122. ... 1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- ...http://jpet.aspetjournals.org/content/305/1/40
JCI - Peroxisome proliferator-activated receptor γ coactivator-1 promotes cardiac mitochondrial biogenesis
Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression. FEBS Lett 1997. 408:39- ... Western blot studies (bottom) were performed with cellular protein extracts prepared from myocytes 4 days after adenoviral ... A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA 1998. 95:2509-2514. View this article via: ... corrected for total cellular protein content (AP , 0.05). The values represent four samples in two independent experiments. (b ...https://www.jci.org/articles/view/10268
Fusion constructs and use of same to produce antibodies with increased Fc receptor binding affinity and effector function -...
The present invention relates to the field of glycosylation engineering of proteins. More particularly, the present invention ... Antibody-dependent cellular cytotoxicity (ADCC) of "M2-GnTIII"-glycoengineered vs. unmodified recombinant, anti-CD20 chimeric ... the pharmaceutical carrier may be a lipid carrier. The lipid carrier may be a phospholipid. Further, the lipid carrier may be a ... Fusion Protein ManII-GalT. A fusion protein (SEQ ID NO:20), consisting of the hManII CTS and the catalytic domain of the human ...http://www.freepatentsonline.com/8859234.html
A Target-Specific Oral Formulation of Doxorubicin-Protein Nanoparticles: Efficacy and Safety in Hepatocellular Cancer
Hawkins MJ, Soon-Shiong P, Desai N. Protein nanoparticles as drug carriers in clinical medicine. Adv Drug Deliv Rev. 2008;60(8 ... Recombinant amphiphilic protein micelles for drug delivery. Langmuir. 2011;27(23):14329-34 ... where the liver tissue sections showed normal cellular morphology (Fig 1C, 1D). The above observations suggest that the protein ... Ionically-crosslinked milk protein nanoparticles as flutamide carriers for effective anticancer activity in prostate cancer- ...http://www.jcancer.org/v04p0644.htm
Patent US5679775 - Process and device for the simultaneous extracorporeal elimination of tumour ... - Google Patents
Abbas, et al., Cellular and Molecular Immunology, 229 232. 1991.. 2. Abbas, et al., Cellular and Molecular Immunology, 229-232 ... However, regarding these proteins (retinol binding protein, ceruloplasmin, prealbumin, IgM) it is known that an endogenous ... Purification of recombinant tumor necrosis factor. US4880915 *. Nov 19, 1985. Nov 14, 1989. Asahi Kasei Kogyo Kabushiki Kaisha ... The carrier of the toxic properties is the lipid A. This substructure which is almost invariable for all lipopolysaccharides ...http://www.google.com/patents/US5679775?dq=6,460,050
Patent US7687069 - Time release patch containing phage associated lytic enzymes for treating ... - Google Patents
The carrier for delivering at least one lytic enzyme to the eye may be but is not limited to the use of an isotonic solution. ... and a carrier for delivering said lytic enzyme. ... Use of gram-positive bacteria to express recombinant proteins. ... Process for isolating cellular components. US5688501. Jan 29, 1996. Nov 18, 1997. Exponential Biotherapies, Inc.. Antibacterial ... Recombinant poxvirus and streptococcal M protein vaccine. US5997862. Oct 31, 1997. Dec 7, 1999. New Horizons Diagnostics ...http://www.google.com/patents/US7687069?dq=6948823
Patent US6685937 - Chewing gum containing phage associated lytic enzymes for treating ... - Google Patents
... an effective amount of lysin enzyme produced by group C streptococcal bacteria infected with a C1 bacteriophage and a carrier ... Recombinant poxvirus and streptococcal M protein vaccine. US5997862 *. Oct 31, 1997. Dec 7, 1999. New Horizons Diagnostics ... The preferred carrier is a tampon, or vaginal douche. A pad may also be used as a carrier, although it is not as effective. ... Process for isolating cellular components. US5688501. Jan 29, 1996. Nov 18, 1997. Exponential Biotherapies, Inc.. Antibacterial ...http://www.google.com/patents/US6685937?dq=6,272,646
Engineering Surfaces for Substrate-Mediated Gene Delivery Using Recombinant Proteins
Cellular debris was removed by centrifugation for 40 minutes at 6000g at 4°C. The protein was purified with Chelating Sepharose ... Bathori G, Cervenak L, Karadi I. Critical Reviews in Therapeutic Drug Carrier Systems. 2004;21(2):67-95. [PubMed] ... Recombinant proteins can be employed in place of full-length extracellular matrix proteins for substrate-mediated gene delivery ... In addition to bioactivity and molecular weight of the proteins, the charge of the recombinant protein coatings may also be ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2765786/
References - Muscular Dystrophy - Fitness VIP
Rose, J.A., Maizel, J.V., Jr., Inman, J.K., and Shatkin, A.J. (1971). Structural proteins of adenovi-rus-associated viruses. J ... Clemens, P.R., Krause, T.L., Chan, S., Korb, K.E., Graham, F.L., and Caskey, C.T. (1995). Recombinant truncated dystrophin ... Nolan, M.A., Jones, O.D., Pedersen, R.L., and Johnston, H.M. (2003). Cardiac assessment in childhood carriers of Duchenne and ... Rando, T.A. (2001). The dystrophin-glycoprotein complex, cellular signaling, and the regulation of cell survival in the ...https://www.fitness-vip.com/muscular-dystrophy/references-xdh.html
Survivin-binding proteins, encoding nucleic acids, and methods of use - The Burnham Institute
... there are provided novel Survivin-binding-proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing ... Various binding assays to identify cellular proteins that interact with protein binding domains are known in the art and ... As used herein, the term "acceptable carrier" encompasses any of the standard pharmaceutical carriers, such as phosphate ... The invention additionally provides recombinant cells containing SBP nucleic acids of the invention. The recombinant cells are ...http://www.freepatentsonline.com/7041783.html
Frontiers | New insights into the targeting of a subset of tail-anchored proteins to the outer mitochondrial membrane | Plant...
TA proteins. This motif is conspicuously absent, however, in all but one of the TA protein subunits of the translocon at the ... TA proteins. This motif is conspicuously absent, however, in all but one of the TA protein subunits of the translocon at the ... The molecular mechanisms by which TA proteins are sorted to the proper organelles are not well understood. Herein we present ... The molecular mechanisms by which TA proteins are sorted to the proper organelles are not well understood. Herein we present ...https://www.frontiersin.org/articles/10.3389/fpls.2014.00426/full