Climb For Life - 4. What is bone marrow hematopoietic stem cell transplant used for?
With the expression "bone marrow hematopoietic stem cell transplant" we intend a complex procedure used especially, but not only, in the treatment of leukimias and lymphomas. Stem cells can be obtained not only from bone marrow but also from peripheral blood after a specific preconditioning of the patient, or from umbilical-cord blood.. Indications for hematopoietic stem cell transplant are acute leukimias, chronic leukimias, different forms of bone marrow insufficiency, thalassemias, Hodgkin lymphoma, non Hodgkin lymphomas, myelomas, other chronic myeloproliferative diseases, numerous genetic disorders and, as a recent indication, some autoimmune illnesses.. ...http://www.climbforlife.it/4-what-is-bone-marrow-hematopoietic-stem-cell-transplant-used-for/
Systemic Virus Infections Differentially Modulate Cell Cycle State and Functionality of Long-Term Hematopoietic Stem Cells In...
Quiescent long-term hematopoietic stem cells (LT-HSCs) are efficiently activated by type I interferon (IFN-I). However, this effect remains poorly investigated in the context of IFN-I-inducing virus infections. Here we report that both vesicular stomatitis virus (VSV) and murine cytomegalovirus (MCMV) infection induce LT-HSC activation that substantially differs from the effects triggered upon injection of synthetic IFN-I-inducing agents. In both infections, inflammatory responses had to exceed local thresholds within the bone marrow to confer LT-HSC cell cycle entry, and IFN-I receptor triggering was not critical for this activation. After resolution of acute MCMV infection, LT-HSCs returned to phenotypic quiescence. However, non-acute MCMV infection induced a sustained inflammatory milieu within the bone marrow that was associated with long-lasting impairment of LT-HSC function. In conclusion, our results ...https://repository.medri.uniri.hr/islandora/object/medri%3A1882
Allometric Scaling of the Active Hematopoietic Stem Cell Pool across Mammals
Background Many biological processes are characterized by allometric relations of the type Y = Y0Mb between an observable Y and body mass M, which pervade at multiple levels of organization. In what regards the hematopoietic stem cell pool, there is experimental evidence that the size of the hematopoietic stem cell pool is conserved in mammals. However, demands for blood cell formation vary across mammals and thus the size of the active stem cell compartment could vary across species. Methodology/Principle Findings Here we investigate the allometric scaling of the hematopoietic system in a large group of mammalian species using reticulocyte counts as a marker of the active stem cell pool. Our model predicts that the total number of active stem cells, in an adult mammal, ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0000002
Fetal liver hematopoietic stem cell niches associate with portal vessels | Science
Whereas the cellular basis of the hematopoietic stem cell (HSC) niche in the bone marrow has been characterized, the nature of the fetal liver niche is not yet elucidated. We show that Nestin+NG2+ pericytes associate with portal vessels, forming a niche promoting HSC expansion. Nestin+NG2+ cells and HSCs scale during development with the fractal branching patterns of portal vessels, tributaries of the umbilical vein. After closure of the umbilical inlet at birth, portal vessels undergo a transition from Neuropilin-1+Ephrin-B2+ artery to EphB4+ vein phenotype, associated with a loss of periportal Nestin+NG2+ cells and emigration of HSCs away from portal vessels. These data support a model in which HSCs are titrated against a periportal vascular niche with a fractal-like organization enabled by placental circulation. ...http://science.sciencemag.org/content/351/6269/176
The only curative therapy for sickle cell disease (SCD) is allogeneic hematopoietic stem cell (HSC) transplantation. Gene therapy approaches for autologous HSC transplantation are being developed. Although earlier engraftment is seen when cells from GCSF-mobilized blood are transplanted than when bone marrow is transplanted, administration of GCSF to patients with SCD can cause significant morbidity. We tested whether primitive hematopoietic progenitors are spontaneously mobilized in the blood of patients with SCD during acute crisis (AC-SCD patients). The frequency of myeloid-lymphoid-initiating cells (ML-ICs) and SCID-repopulating cells (SRCs) was significantly higher in blood from AC-SCD ...https://www.jci.org/111/6
Ex vivo expansion of cord blood progenitors impairs their short-term and long-term repopulating activity associated with...
Holmes, T., Yan, F., Ko, K.-H., Nordon, R., Song, E., O'Brien, T. A. and Dolnikov, A. (2012), Ex vivo expansion of cord blood progenitors impairs their short-term and long-term repopulating activity associated with transcriptional dysregulation of signalling networks. Cell Proliferation, 45: 266-278. doi: 10.1111/j.1365-2184.2012.00813.x ...http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2184.2012.00813.x/references
Identification and characterization of genes specifically expressed in hematopoietic progenitor/stem cells immortalized by Lhx2
During embryonic development a variety of tissues and organs such as the lung, eye, and kidney are being formed. The generation of functional organs is regulated by reciprocal cell-cell interactions. Via the secretion of soluble molecules one type of cells affect the fate of their neighboring cells. A central issue in organogenesis is how a cell interprets such extrinsic signals and adopts a specific fate, and how the cell in response to this signal establishes reciprocal signaling. Transcription factors play a critical role in this process and my thesis focuses on the role of the LIM-homeodomain transcription factor, Lhx2, in the development of three different organ systems, the liver, the hematopoietic system and the olfactory system.. The liver is formed from endoderm of the ventral foregut and mesenchyme ...http://umu.diva-portal.org/smash/record.jsf?pid=diva2:143023
"Hemopoietic precursor cells of the mouse spleen incapable of self- -re" by M Bennett and G Cudkowicz
Bennett, M and Cudkowicz, G, "Hemopoietic precursor cells of the mouse spleen incapable of self- -replication. Abstr." (1967). Subject Strain Bibliography 1967. 1376 ...https://mouseion.jax.org/ssbb1967/1376/
In vitro expansion of murine hematopoietic progenitor cells in liquid cultures for bone marrow transplantation: effects of stem...
In this study, we demonstrated that recombinant stem cell factor (SCF), when used in combination with interleukin-1 (IL-1), IL-3 and IL-1 plus IL-3, promoted the expansion of immature murine hematopoietic progenitor cells in liquid cultures. In cultures with IL-1 plus IL-3 plus SCF, high proliferati...http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART00105598
Haematopoietic progenitor cells utilise conventional PKC to suppress PKB/Akt activity in response to c-Kit stimulation
Receptor tyrosine kinase (RTK) c-Kit signalling is crucial for the proliferation, survival and differentiation of haematopoietic stem cells (HSCs). To further understand the mechanisms underlying these events we explored how the downstream mediators interact. The present study investigated the function of conventional protein kinase Cs (c-PKC) in c-Kit mediated signalling pathways in HSC-like cell lines. This analysis supported earlier findings, that steel factor (SF) activates c-PKC, extracellular signal-regulated kinase (Erk) and protein kinase B (PKB). The present results were consistent with an important role of c-PKC in the positive activation of Erk and for proliferation. Further, it was observed that c-PKC negatively regulated PKB activity upon SF stimulation, indicating that c-PKC acts as a suppressor of c-Kit signalling. Finally, these observations were extended to show that c-PKC mediated the phosphorylation of the ...http://umu.diva-portal.org/smash/record.jsf?pid=diva2:145584
Stem Cells for Myocardial Regeneration | Circulation Research
Two categories of blood-forming stem cells exist in adult bone marrow. One population can provide permanent long-term reconstitution of the entire hematopoietic system. These cells, referred to as hematopoietic stem cells (HSCs), are rare, perhaps as few as 1:10 000 bone marrow cells.28 However, utilizing the specificity of monoclonal antibodies, HSCs can be enriched by flow cytometry to near purity on the basis of surface markers. As few as 20 to 100 highly purified mouse bone marrow HSCs can reconstitute the entire lymphohematopoietic system in myeloablated adult mice.29-31 They can self-renew and can ...http://circres.ahajournals.org/content/91/12/1092
Inflammation-Mediated Notch Signaling Skews Fanconi Anemia Hematopoietic Stem Cell Differentiation | The Journal of Immunology
The current study identifies a potential interaction between the FA pathway and Notch signaling in HSC differentiation and establishes a role of FA proteins in the control of balance between renewal and lineage commitment. There are several findings that highlight the significance of our study: 1) loss of murine FA proteins results in enhanced Notch signaling in MPPs, which is correlated with decreased phenotypic and functional LT-HSCs and increased formation of MPP1 progenitors; 2) deletion of the Fanca or Fancc gene deregulates genes in the Notch signaling and the NF-κB pathway; 3) TNF-α stimulation enhances Notch signaling in Fanca−/− and Fancc−/− LSK cells, leading to decreased HSC quiescence and compromised HSC self-renewal; 4) inflammation-activated Notch target gene expression in Fanca−/− and Fancc−/− MPP cells requires NF-κB; 5) genetic ablation or pharmacologic ...http://www.jimmunol.org/content/191/5/2806.long
Cdx4 is dispensable for murine adult hematopoietic stem cells but promotes MLL-AF9-mediated leukemogenesis | Haematologica
Background Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior hox genes. However, its role in adult mammalian hematopoiesis has not been extensively studied and its requirement in leukemia associated with Hox gene expression alteration is unclear. Design and Methods We inactivated Cdx4 in mice through either a germline or conditional knockout approach and analyzed requirement for Cdx4 in both normal adult hematopoiesis and leukemogenesis initiated by the MLL-AF9 fusion oncogene. Results Here, we report that loss of Cdx4 had minimal effect on adult hematopoiesis. Indeed, although an increase in white blood cell counts was observed, no significant difference in the distribution of mature blood cells, progenitors or stem ...http://www.haematologica.org/content/early/2010/05/21/haematol.2010.023168
Regulation Of Blood System, Regulation of Blood System Products, Regulation of Blood System Manufacturers, Regulation of Blood...
Regulation Of Blood System, Find Quality Regulation of Blood System Products, Regulation of Blood System Manufacturers, Regulation of Blood System Suppliers and Exporters at Alibaba.com.http://www.alibaba.com/Regulation-of-Blood-System_pid100003214_2
Translational Research: The CD34+ Cell Is Crucial for Large-Volume Bone Regeneration from the Milieu of Bone Marrow Progenitor...
DOI: 10.11607/jomi.te56 Purpose: This study investigated the role of the bone marrow derived CD34+ cell in a milieu of osteoprogenitor cells, bone marrow plasma cell adhesion molecules, recombinant human bone morphogenetic protein (rhBMP), and a matrix of crushed cancellous allogeneic bone in the clinical regeneration of functionally useful bone in craniomandibular reconstructions. The history and current concepts of bone marrow hematopoietic stem cells and mesenchymal stem cells are reviewed as they relate to bone regeneration in large continuity defects of the mandible. Materials and Methods: Patients with 6- to 8-cm continuity defects of the mandible with retained proximal and distal segments were randomized into two groups. Group A received an in situ ...http://www.quintpub.com/journals/omi/abstract.php?iss2_id=1221&article_id=14314&article=32&title=Translational%20Research:%20The%20CD34+%20Cell%20Is%20Crucial%20for%20Large-Volume%20Bone%20Regeneration%20from%20the%20Milieu%20of%20Bone%20Marrow%20Progenitor%20Cells%20in%20Craniomandibular%20Reconstruction
"Effect of cyclophosphamide on the murine hematopoietic stem cell compa" by S Hellman and H E. Grate
Hellman, S and Grate, H E., "Effect of cyclophosphamide on the murine hematopoietic stem cell compartment as measured by different assay techniques." (1971). Subject Strain Bibliography 1971. 1381 ...https://mouseion.jax.org/ssbb1971/1381/
Oncogenic transcription factor Evi1 regulates hematopoietic stem cell proliferation through GATA‐2 expression | The EMBO Journal
We next examined whether Evi1 could regulate the transcription of GATA‐2 through direct binding to the binding sequence b for D1 (D1‐b) or five other binding sequences for D2 (D2‐f, ‐g, ‐h, ‐j and ‐l) (Figure 7A) using hematopoietic cell lines. Various genomic fragments of the GATA‐2 IS promoter region were isolated and inserted upstream of luciferase cDNA (Luc) in the pGL3‐Basic vector (Figure 7A). Each reporter plasmid was transiently transfected into EML C1, a murine stem cell factor‐dependent multipotent progenitor cell line (Tsai et al, 1994), or HEL, a human erythroleukemia cell line (Martin and Papayannopoulou, 1982). GATA‐2 mRNA is highly expressed in both cell lines, while the Evi1 mRNA level is higher in HEL but relatively low in EML C1 (data not shown). In HEL cells, the luciferase activity was detected only with the pGL3‐B7.0 plasmid, suggesting that it was primarily driven by DNA derived from a ...http://emboj.embopress.org/content/24/11/1976
Smurf2 regulates hematopoietic stem cell self-renewal and agin...
Smurf2 regulates hematopoietic stem cell self-renewal and aging.: The age-dependent decline in the self-renewal capacity of stem cells plays a critical role inhttps://www.mysciencework.com/publication/show/smurf2-regulates-hematopoietic-stem-cell-self-renewal-aging-efb689c1
X-irradiation of purified primary cultures of mouse bone marrow stroma or permanent cloned marrow stromal cell lines in plateau phase decreases production of macrophage progenitor cell-specific colony-stimulating factor to a plateau minimum of 40% of control levels after doses of 50 to 500 Gy delivered at 2 Gy/min. After 50 Gy there is increased bioavailability of another growth factor(s) that is distinct from macrophage progenitor cell-specific colony-stimulating factor, granulocytemacrophage progenitor cell colony-stimulating factor, or colony-stimulating factor for multipotential hematopoietic stem cells (interleukin 3). Liquid-phase cocultivation of irradiated stromal cells with either nonadherent cells from continuous marrow cultures or cloned dual granulocytemacrophage ...http://cancerres.aacrjournals.org/content/46/9/4677
Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term...
In this study, we show that the amount of Evi1 transcript can be indicative of an undifferentiated state with multipotent differentiation capacity within HSPCs. In both the fetal and adult hematopoietic systems, Evi1 expression can mark long-term multilineage repopulating HSCs, and enhance HSC purification with a combination of other surface markers, suggesting a specific relationship between HSC activity and Evi1 expression throughout ontogeny. This stem cell-specific expression pattern of Evi1 allows us to functionally identify self-renewing HSCs by using Evi1-IRES-GFP knock-in mice, and suggests the relevance of Evi1 in fine-tuning of stem cell properties. Indeed, we provide the genetic evidence confirming that Evi1 has a predominant effect on LT-HSCs by specifically regulating their self-renewal capacity.. The prospective isolation of HSCs is the most important step to dissect their function. The strategy commonly used for HSC isolation ...http://jem.rupress.org/content/early/2011/11/10/jem.20110447
Download Predicting The Lineage Choice Of Hematopoietic Stem Cells A Novel Approach Using Deep Neural Networks
download predicting the lineage choice of hematopoietic stem history for drift child in specimen applications: a FREE series of scalpel subjects. download predicting the of right function to Object Cryopreservation in available greens with disease zip. nutrient download predicting the lineage choice of hematopoietic stem to journalist: minutes and matter-of-fact contrast. Panellists of warm interested new download predicting the lineage choice of hematopoietic stem: separate addition dignity levels and inflammatory period. download predicting the lineage choice of hematopoietic stem support in filaments with heat field. download predicting in impossible immune intervention bacteriuria. pulmonary download predicting the lineage choice of hematopoietic stem cells a novel ...http://harveyphillipsfoundation.org/img/library/download-predicting-the-lineage-choice-of-hematopoietic-stem-cells%3A-a-novel-approach-using-deep-neural-networks.htm
Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. - Semantic Scholar
The cellular constituents forming the haematopoietic stem cell (HSC) niche in the bone marrow are unclear, with studies implicating osteoblasts, endothelial and perivascular cells. Here we demonstrate that mesenchymal stem cells (MSCs), identified using nestin expression, constitute an essential HSC niche component. Nestin(+) MSCs contain all the bone-marrow colony-forming-unit fibroblastic activity and can be propagated as non-adherent 'mesenspheres' that can self-renew and expand in serial transplantations. Nestin(+) MSCs are spatially associated with HSCs and adrenergic nerve fibres, and highly express HSC maintenance genes. These genes, and others triggering osteoblastic differentiation, are selectively downregulated during enforced HSC mobilization or beta3 adrenoreceptor activation. Whereas parathormone administration doubles the ...https://www.semanticscholar.org/paper/Mesenchymal-and-haematopoietic-stem-cells-form-a-u-M%C3%A9ndez-Ferrer-Michurina/4c008d7f62d031755b291dbde076d4c86c8e9671
Role of PGE2 and other eicosanoids in hematopoietic stem cell function - Louis Pelus
Hematopoietic stem cell transplant is a potentially curative therapy for hematologic and non-hematologic and genetic disorders;however limitations in stem cell...http://grantome.com/grant/NIH/R01-HL096305-04
CFU-GEMM - Wikipedia
CFU-GEMM is a colony forming unit that generates myeloid cells. CFU-GEMM cells are the multipotential progenitor cells for myeloid cells; they are thus also called common myeloid progenitor cells or myeloid stem cells. "GEMM" stands for granulocyte, erythrocyte, monocyte, megakaryocyte. The common myeloid progenitor (CMP) and the common lymphoid progenitor (CLP) are the first branch of cell differentiation in hematopoiesis after the hemocytoblast (hematopoietic stem cell). In current terminology, CFU-S refers to the pluripotent ...https://en.wikipedia.org/wiki/CFU-GEMM
Lymphoid-restricted development from multipotent candidate murine stem cells: distinct and complimentary functions of the c-kit...
The two tyrosine kinase receptors, c-kit and flt3, and their respective ligands KL and FL, have been demonstrated to play key and nonredundant roles in regulating the earliest events in hematopoiesis. However, their precise roles and potential interactions in promoting early lymphoid commitment and development remain unclear. Here we show that most if not all murine Lin(-/lo)Sca1(+)c-kit(+) bone marrow (BM) cells generating B220(+)CD19(+) proB-cells in response to FL and interleukin-7 (IL-7) also have a myeloid potential. In contrast to FL + IL-7, KL + IL-7 could not promote proB-cell formation from Lin(-/lo)Sca1(+)c-kit(+) cells. However, KL potently enhanced FL + IL-7-stimulated proB-cell formation, in part through enhanced recruitment of FL + IL-7-unresponsive Lin(-/lo)Sca1(+)c-kit(+) ...https://www.rdm.ox.ac.uk/publications/573551