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*  Cell culture - Wikipedia
Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium ... into immortal cells which will reproduce indefinitely if the optimal conditions are provided. In practice, the term "cell ... Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Microcarrier Cell ... and the cells that grow out are available for culture. This method is known as explant culture. Cells that are cultured ...
  https://en.wikipedia.org/wiki/Cell_culture
*  Monoclonal antibody - Wikipedia
The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... Culture-media conditioned by macrophages can be used. Production in cell culture is usually preferred as the ascites technique ... Only fused hybrid cells, referred to as hybridomas, are able to grow indefinitely in the media because the spleen cell partner ... single B cell culture, single cell amplification from various B cell populations and single plasma cell interrogation ...
  https://en.wikipedia.org/wiki/Monoclonal_antibody
*  Bizarre tumor case may lead to custom cancer care | LAMonitor.com
That enabled healthy and cancerous cells to keep growing indefinitely.. Researchers grew 'mini tumors' from the man's lung mass ... adding mouse cells for nourishment, plus a chemical that prevents cell death to an ordinary lab culture medium. ... The condition causes wartlike growths in the throat, usually around the voice box. These growths usually are noncancerous but ... What could be more personalized than taking this person's cell, growing it in culture, finding a drug to treat them and then ...
  http://www.lamonitor.com/content/bizarre-tumor-case-may-lead-custom-cancer-care?quicktabs_2=0&mini=calendar-date%2F2013-07
*  Bacteria - encyclopedia article - Citizendium
Nearly all cultures start from taking a relatively old stock of bacteria and diluting them in to fresh media; these cells need ... The rate at which cells grow during this phase is known as the growth rate (k). The time it takes the cells to double during ... These limitations can be avoided by the use of a chemostat, which maintains a bacterial culture under steady-state conditions ... In a bacterium's natural environment nutrients are limited, meaning that bacteria cannot continue to reproduce indefinitely. ...
  http://en.citizendium.org/wiki/Bacteria
*  Cell culture facts, information, pictures | Encyclopedia.com articles about Cell culture
Make research projects and school reports about Cell culture easy with credible articles from our FREE, online encyclopedia and ... and pictures about Cell culture at Encyclopedia.com. ... Cancer cell lines, which can grow indefinitely in culture, also ... cell culture The culturing of cells from plants, animals, or microorganisms on a culture medium in vitro. See culture.. ... Accordingly, most culture methods require sterile conditions. Often antibiotics are used to inhibit growth of unwanted ...
  http://www.encyclopedia.com/science-and-technology/biology-and-genetics/cell-biology/cell-culture
*  What is Plant Tissue Culture
... liquid medium called a suspension culture. These cells can be maintained indefinitely provided they are sub-cultured regularly ... aseptic conditions during culture to exclude competition from microorganisms The plant cells can grow on a solid surface as ... culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells ... The exact conditions required to initiate and sustain plant cells in culture, or to regenerate intact plants from cultured ...
  https://www.liverpool.ac.uk/~sd21/tisscult/what.htm
*  Induced stem cells - Wikipedia
"Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". ... added to the cell culture medium, show that it may be possible to reclone animals indefinitely with no visible accumulation of ... grown from the pluripotent stem cells in vivo in the animal, these cells should express two molecules: CTLA4-Ig, which disrupts ... can be cultured indefinitely as conditionally reprogrammed cells (CRCs). Cell surface progenitor-associated markers are rapidly ...
  https://en.wikipedia.org/wiki/Induced_stem_cells
*  Trichomitus batrachorum (Perty) Honigberg ATCC ® 30066™
1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a ... Medium ATCC® Medium 719: Modified TYM basal medium with pH adjusted to 7.2. ... Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively ... Growth Conditions Temperature: 25.0°C. Duration: axenic; anaerobic. Cryopreservation ...
  https://www.atcc.org/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/30066.aspx
*  Tissue culture, Tissue Culture It is an important technique for maintaining a'pm or, Biology
It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. ... Tissue Culture It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture ... These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some ... in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an ...
  http://www.expertsmind.com/questions/tissue-culture-30124936.aspx
*  Synechocystis - Wikipedia
PCC6803 can be grown on either agar plates or in liquid culture. The most widely used culture medium is a BG-11 salt solution. ... Cultures can be kept on agar plates for approximately 2 weeks and re-streaked indefinitely. For long term storage, liquid cell ... Some glycolytic genes are regulated by the genesll1330 under light and glucose-supplemented conditions. One of the most ... Group 1 sigma factors are critical for cell viability. Group 2, similar in structure to Group 1, is not essential for cell ...
  https://en.wikipedia.org/wiki/Synechocystis
*  Applications of Biotechnology to Traditional Fermented Foods (BOSTID, 1992, 188 p.): II. Overview: 6 Mixed-Culture Fermentations
... to grow a mixed culture from liquid medium in contrast to typical fermentations on solid mediums, without the culture ... Mixed cultures can be maintained indefinitely by unskilled people with a minimum of training. If the environmental conditions ... The number of S. thermophilus cells increased from 500 x 106 per milliliter to 880 x 106 per milliliter with L. bulgaricus. · ... the best way to preserve mixed cultures is to store the whole liquid culture in liquid nitrogen below -80°C. The culture, when ...
  http://www.nzdl.org/gsdlmod?e=d-00000-00---off-0edudev--00-0----0-10-0---0---0direct-10---4-------0-0l--11-en-50---20-help---00-0-1-00-0--4----0-0-11-10-0utfZz-8-10&a=d&c=edudev&cl=CL1.1&d=HASH01ffca36ef9be9ebbab06841.5.6
*  Bacteria - wikidoc
Specimens that are normally sterile, such as blood, urine or spinal fluid, are cultured under conditions designed to grow all ... Growth in stirred liquid media occurs as an even cell suspension, making the cultures easy to divide and transfer, although ... However, in natural environments nutrients are limited, meaning that bacteria cannot continue to reproduce indefinitely. This ... Further information: [[:Cell envelope]]. Around the outside of the cell membrane is the bacterial cell wall. Bacterial cell ...
  https://www.wikidoc.org/index.php/Bacterium
*  Tritrichomonas foetus (Riedmuller) Wenrich and Emerson ATCC ® 3023
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. When cells grown in a ... Medium ATCC® Medium 2154: LYI Entamoeba medium. ATCC® Medium 359: Modified TYM basal medium (ATCC medium 358) with pH adjusted ... Storage Conditions Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. ... Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/30231.aspx
*  Introduction to Cell Culture | Thermo Fisher Scientific - MX
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favoriable artificial ... adherent or monolayer culture), while others can be grown floating in the culture medium (suspension culture).. ... Culture Conditions Culture conditions vary widely for each cell type, but the artificial environment in which the cells are ... When a finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes a continuous cell ...
  https://www.thermofisher.com/mx/en/home/references/gibco-cell-culture-basics/introduction-to-cell-culture.html
*  Cultured Arterial Smooth Muscle Cells Maintain Distinct Phenotypes When Implanted Into Carotid Artery | Arteriosclerosis,...
When media-derived SMC populations are placed in culture, they tend to proliferate indefinitely in the presence of serum.21 ... 21 thus providing evidence that in normal conditions, the arterial media contains cells capable of developing in vitro the 2 ... 8 Cells were grown in DMEM (GIBCO) supplemented with 10% FCS (Hyclone) and used between passages 5 and 10. Cells were ... 3 cells per 104 μm2 and 76±4 cells per 104 μm2, respectively, for newborn rats, P,0.05; 59±2 cells per 104 μm2 and 84±6 cells ...
  http://atvb.ahajournals.org/content/21/6/949
*  Bacteria - Wikipedia
Growth in stirred liquid media occurs as an even cell suspension, making the cultures easy to divide and transfer, although ... Specimens that are normally sterile, such as blood, urine or spinal fluid, are cultured under conditions designed to grow all ... However, in natural environments, nutrients are limited, meaning that bacteria cannot continue to reproduce indefinitely. This ... Solid growth media, such as agar plates, are used to isolate pure cultures of a bacterial strain. However, liquid growth media ...
  https://en.wikipedia.org/wiki/Bacteria
*  Development of Approaches to Improve Cell Survival in Myoblast Transfer Therapy
The immortalized mdx cell line proliferated indefinitely under the permissive conditions of 33°C with gamma interferon ( ... the fusion medium was F10-Ham supplemented with 2% FBS and 1% antibiotic solution (penicillin/ streptomycin). All the culture ... The cells emerging from these myofibers were grown until confluence, assayed for desmin expression, transduced with adenovirus ... The myoblast cell line (cell line) and the highly pure myoblast culture isolated from myofibers (FMb) also display a reduction ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2149359/
*  Patent US7468458 - Accelerants for the modification of non-natural amino acids and non-natural ... - Google Patents
... and cell contents. Thus, the term may encompass medium in which the host cell has been grown, e.g., medium into which the ... under physiological conditions for an extended period of time, perhaps even indefinitely. Hydrolytically unstable or degradable ... As used herein, the term "medium" or "media" includes any culture medium, solution, solid, semi-solid, or rigid support that ... insect host cells, plant host cells, eukaryotic host cells, mammalian host cells, CHO cells, prokaryotic host cells, E. coli, ...
  http://www.google.com/patents/US7468458
*  Immortal organisms? | Physics Forums - The Fusion of Science and Community
... simply by altering the culture conditions, such as the medium or serum. For example, it is possible to select HeLa cells that ... That would depend on environmental conditions and cells. In the laboratory you can grow cells under identical conditions, so ... I would carry on with my normal reasonably good health and cell turnover indefinitely as if I were in my twenties. Within a few ... provided that the cells are not grown continuously - instead, the cells should be replenished from frozen stocks every few ...
  https://www.physicsforums.com/threads/immortal-organisms.392207/
*  Trichomonas vaginalis Donne ATCC ® 30239™
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a ... Medium ATCC® Medium 2154: LYI Entamoeba medium. ATCC® Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted ... Storage Conditions Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. ... Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Parasitic_Protozoa/30239.aspx
*  Subculture (biology) - Wikipedia
Adherent cells, for example many mammalian cell lines, grow attached to a surface such as the bottom of the culture flask. ... is a new cell or microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium ... Cell lines and microorganisms cannot be held in culture indefinitely due to the gradual rise in toxic metabolites, use of ... For microorganisms there is a tendency to adapt to culture conditions, which is rarely precisely like the microorganism's ...
  https://en.wikipedia.org/wiki/Subculture_(biology)
*  Cultured meat - Wikipedia
Culture medium: Proliferating cells need a food source to grow and develop. The growth medium should be a well-balanced mixture ... The same improvement can be made without genetic modification, by manipulating the conditions of the culture medium. Genetic ... it would be theoretically possible to continue producing meat indefinitely without introducing new cells from a living organism ... Such cells include embryonic stem cells, adult stem cells, myosatellite cells, or myoblasts. Stem cells proliferate the ...
  https://en.wikipedia.org/wiki/Cultured_meat
*  WO1995005464A1 - B7-2: ctla4/cd28 counter receptor - Google Patents
... the recombinant host cells including either the sequences or vectors, and recombinant polypeptides. The invention includes as ... recombinant polypeptides in assays designed to select and improve substances capable of altering T cell activation for use in ... and regulates T cell activation. The invention relates as well to the polynucleotides sequences encoding those polypeptides, ... transfected cells are maintained under culture conditions in a culture medium.. Suitable medium for various cell types are well ...
  https://patents.google.com/patent/WO1995005464A1/en
*  SVEC4-10 ATCC ® CRL-2181™ Mus musculus axillary lymph node/
... transformation of endothelial cells from axillary lymph node vessels. ... SVEC4-10 is an endothelial cell line derived by SV40 (strain 4A) ... Culture medium, 95%; DMSO, 5%. Culture Conditions Temperature: ... They grow indefinitely without special additives and are well differentiated, responding like normal endothelial cells to some ... Remove and discard culture medium.. *Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all ...
  https://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Tissue/Lymph_Node/CRL-2181.aspx?p=1&rel=%7B0%7D
*  Chemostat | Article about chemostat by The Free Dictionary
The nutrients required for cell growth are supplied continuously to the... Explanation of chemostat ... An apparatus for the continuous cultivation of microorganisms or plant cells. ... Large scale 3-D cell/tissue culturing: the challenge: how to grow cells in high densities over a long period of time ... The nutrients required for cell growth are supplied continuously to the culture vessel by a pump connected to a medium ...
  http://encyclopedia2.thefreedictionary.com/Chemostat