Abstract 707: Tumor Necrosis Factor-alpha p75 Receptor, Sattelite-Cell Activation and Neovascularization | Circulation
... in the expression of VEGF and bFGF2 mRNA transcripts in ischemic tissue and in circulating endothelial progenitor cells (EPCs ... whereas in p75KOs after day 1 and through day 10 sattelite cells were not detecable. To the contrary, p75KO tissue showed ... Compared to presurgery, on days 1-10 post-HL surgery there was 6 - 8-fold increase in the number of sattelite-cells (as shown ... Abstract 707: Tumor Necrosis Factor-alpha p75 Receptor, Sattelite-Cell Activation and Neovascularization. David A Goukassian, ...http://circ.ahajournals.org/content/114/Suppl_18/II_120.4
Castration-induced reduction of vascular endothelial growth factor expression in benign human prostate tissue is lost in...
VEGF mRNA was down-regulated after castration in benign prostate tissue (P , or = 0.05), whereas in tumour tissue, VEGF levels ... mRNA expression in benign prostate tissue and generally in those prostate tumours where castration also induces tumour cell ... MATERIALS AND METHODS: The expression of vascular endothelial growth factor (VEGF) and its receptors was analysed using the ... In most patients whose tumour tissue responded with VEGF reduction, there was a corresponding increase in tumour cell apoptosis ...http://umu.diva-portal.org/smash/record.jsf?pid=diva2:753521
HPGD | Cancer Genetics Web
The role of PGT in mRNA and protein expression of vascular endothelial growth factor (VEGF) was examined in gastric cancer ... The tumor tissues in combination treatment presented more apoptotic cells and less microvessel density. Notably, the number of ... Transfection of AGS and MKN7 gastric cancer cells with PGT-specific siRNA led to increased VEGF mRNA and protein expression ... It had a significant suppressive effect on mRNA levels and on the secretion of PRL and GH in GH3 cells. The inhibition of cell ...http://www.cancerindex.org/geneweb/HPGD.htm
Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
... biological response modifiers and the family of cell adhesion-promoting molecules. ... and vascular endothelial growth factor (VEGF) in PDL cells on mRNA and protein level. This was accomplished by ... and VEGF expression. Immunohistochemistry performed on tissue samples of gingivitis and periodontitis displayed an increase of ... To conclude, the present study underlines the significance of NF-κB and HIF-1α and their target genes VEGF, IL-1β, and MMP-1 in ...https://www.hindawi.com/journals/mi/2015/438085/abs/
VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment
... cell biology and pharmacology of inflammation, including acute/chronic inflammation, mediators of inflammation, as well as ... In endothelial cells, treatment with VEGF results in an increase of both ICAM-1 mRNA and protein expression . Moreover, ... Moreover, endothelial VEGFR-2 staining intensity correlated with the expression level of tissue ICAM-1 (. ). Additionally, ... VEGF mRNA expression, VEGFR-2 expression, and macrophage infiltration [39, 41, 42]. ...https://www.hindawi.com/journals/iji/2015/385030/
Vascular Endothelial Growth Factors (VEGF) | Sigma-Aldrich
In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization. ... VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell ... VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common ... Local hypoxia is a potent inducer of VEGF-A expression from adjacent cells but it is not synthesized in endothelial cells, ...https://www.sigmaaldrich.com/technical-documents/articles/biofiles/vascular-endothelial.html
Characterization of platelet-derived growth factor-C (PDGF-C): expression in normal and tumor cells, biological activity and...
VEGF) family. PDGF-C mRNA is widely expressed in normal tissues and does not appear to be up-regulated in the tumor cell lines ... were able to stimulate proliferation of human artery smooth muscle cells, but were inactive on umbilical vein endothelial cells ... osteoblasts, fibroblasts, skeletal muscle cells (SkMC), bovine chondrocytes, and rat myocardium cells. Although the mitogenic ... contains an N-terminal CUB-like domain and a C-terminal domain with homology to members of the PDGF/vascular endothelial growth ...https://www.semanticscholar.org/paper/Characterization-of-platelet-derived-growth-in-and-Dijkmans-Xu/3f978d7654296afb13f613da32342452a8880dbe
Abstract 5428: Nuclear Factor Kappa B Mediates Postnatal Alveolarization by Promoting Pulmonary Angiogenesis via Vascular...
Pulmonary endothelial cells (EC) were isolated using CD31 coated beads to determine VEGFR2 expression and VEGF-mediated ... At 8h, NFκB blockade (BAY) decreased VEGFR2 mRNA and protein in the lung (p,0.01, versus PBS). At 24h, lungs of BAY treated ... was performed on lung tissue to evaluate if the NFκB subunit, p65, binds the VEGFR2 promoter. ... Furthermore, NFκB blockade with BAY caused a dose-dependent reduction of VEGFR2 mRNA and protein in pulmonary EC, and impaired ...http://circ.ahajournals.org/content/120/Suppl_18/S1090.4
VEGI-192, a New Isoform of TNFSF15, Specifically Eliminates Tumor Vascular Endothelial Cells and Suppresses Tumor Growth |...
... as an example that the expression pattern of VEGI is highly similar to that of endothelial cell marker CD31 as well as VEGF, of ... We showed previously that VEGI mRNA is readily detectable in a variety of human organs and tissues (5). We show here with ... an endothelial cell marker, or that of VEGF-A, a growth factor whose receptors are specific to endothelial cells. Because this ... Vascular endothelial cell-specific localization of vascular endothelial cell growth inhibitor. We have developed a mouse ...http://clincancerres.aacrjournals.org/content/11/15/5595
Frontiers | Differential Angiogenic Gene Expression in TP53 Wild-Type and Mutant Ovarian Cancer Cell Lines | Oncology
Methods: Affymetrix U133A arrays were analyzed for angiogenic gene expression in 19 ovarian cancer cell lines stratified both ... Affymetrix U133A arrays were analyzed for angiogenic gene expression in 19 ovarian cancer cell lines stratified both by TP53 ... mutated (m) TP53 cell lines after treatment under hypoxic conditions or with ionizing radiation. Results: Twenty-eight ... mutated (m) TP53 cell lines after treatment under hypoxic conditions or with ionizing radiation. Results: Twenty-eight ...https://www.frontiersin.org/articles/10.3389/fonc.2014.00163/full
Fibrinogen Concentration Influence on VEGF-Induced Differentiation of Adipose Tissue-Derived Stem Cells into Endothelial Cells
ECs-specific gene expression, and F-actin assembly. During the first 12 h after seeding, cells spread randomly, moved and ... ASCs expressed mRNA and protein of von Willebrand factor (vWF). The degree of ECs differentiation of ASCs was consistently ... Adipose tissue represents an abundant, practical source of donor tissue for stem cells which may be a useful source for ... However, the optimal substrates that promote differentiation of adipose tissue-derived stem cells (ASCs) into ECs remain to be ...https://www.scientific.net/KEM.342-343.17
Endothelial stem cell - Wikipedia
Lymphatic-specific vascular endothelial growth factors VEGF-C and VEGF-D function as ligands for the vascular endothelial ... A study showed that ectopic expression of Prox-1 in blood vascular ECs (BECs) induced one-third of LEC specific gene expression ... which is a booming field that is now working on transplanting cells as opposed to bigger tissues or organs. Understanding more ... Prox-1is a homeobox transcription factor found in lymphatic ECs (LECs). For example, specific mRNAs such as VEGFR-3 and p57Kip2 ...https://en.wikipedia.org/wiki/Endothelial_stem_cell
VEGF is a potent agonist of angiogenesis that activates both endothelial cell proliferation and migration. By contract, TSP-1 ... Presence of diabetes also significantly suppressed the expression of VEGF mRNA in RAGE−/− mice. Other proangiogenic factors, ... and cell migration (28). In the present system, tissue factor mRNA was also suppressed by diabetes in both wild-type and RAGE ... Our present quantitative analyses for mRNA expression revealed that VEGF is decreased while TSP-1 is upregulated in the ...http://diabetes.diabetesjournals.org/content/55/8/2245
Mechanisms Underlying the Impairment of Ischemia-Induced Neovascularization in Matrix Metalloproteinase 2-Deficient Mice |...
... the expression of VEGF mRNA as well as VEGF protein levels were decreased in the local ischemic tissues of MMP-2−/− compared ... Aorta-derived endothelial cells or bone marrow-derived endothelial progenitor cell (EPC)-like c-Kit+ cells from MMP-2−/− showed ... C and D, The expression of VEGF mRNA and of protein decreased in the ischemic tissues of MMP-2−/− mice at day 7. E, ELISA ... In addition, the data on the decrease in VEGF receptor Flt-1 mRNA expression in ischemic tissue of MMP-2−/− mice provided ...http://circres.ahajournals.org/content/100/6/904
Frontiers | The Fibrin Cleavage Product Bβ15-42 Channels Endothelial and Tubular Regeneration in the Post-Acute Course During...
Regarding the angiogenetic players VEGF-A, Angiopoietin-1, Angiopoietin-2, however, we observed significant higher expressions ... Endothelial activation and inflammatory response was attenuated in renal tissue homogenates by single application of Bβ15-42. ... These results suggest a beneficial effect of anatomical contiguously located endothelial cells on tubular regeneration through ... we observed significant higher expressions at mRNA and trend to higher protein level in Bβ15-42 treated mice, compared to ...https://www.frontiersin.org/articles/10.3389/fphar.2018.00369/abstract
MIF (macrophage migration inhibitory factor (glycosylation-inhibiting factor))
Normal Tissue Atlas] [carcinoma Classsification] [NCI60]. Genevestigator. Expression in : [tissues] [cell-lines] [cancer] [ ... expression in human glioblastomas correlates with vascular endothelial growth factor (VEGF) expression.. ... using RT-PCR and immmunohistochemistry and they were able to show a strong correlation between MIF expression and VEGF mRNA ... cell surface receptor signaling pathway cell aging cell proliferation cell surface negative regulation of gene expression ...http://atlasgeneticsoncology.org/Genes/MIFID41365ch22q11.html
Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members
Vascular endothelial growth factor gene expression in colon cancer cells exposed to prostaglandin E2 is mediated by hypoxia- ... Fewer studies addressed the mRNA expression levels in colon cancer of the other VEGF family members: VEGF-B, VEGF-C, VEGF-D and ... Relative mRNA expression levels of VEGF-A (A), VEGF-B (B), VEGF-C (C), VEGF-D (D) and PlGF (E) are shown for healthy colon ... However, besides VEGF-A also other VEGF family members showed significant mRNA expression increments in resected healthy tissue ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3534223/?lang=en-ca
NASA - Commercial Biomedical Test Module - 2
These decreased levels of VEGF are believed to be due to decreased expression of the VEGF mRNA within the ovarian cells. This ... Researchers analyzed mouse tissues after spaceflight and found a broad spectrum of effects: decreased expression of muscle cell ... Previous data has indicated decreased vascular endothelial growth factor (VEGF, signaling protein involved in formation of the ... and gene expression of tumor-attacking cells; reductions in liver, spleen, and thymus masses; changes in mRNA expression of ...https://www.nasa.gov/mission_pages/station/research/experiments/201.html
In contrast to the SW620 xenografts, PKG had no effect on VEGF expression in SW620 cells grown under standard tissue culture ... VEGF expression observed when the cells were subjected to hypoxic stress was significantly attenuated by PKG at both the mRNA ... and was associated with reduced levels of vascular-endothelial growth factor (VEGF). In order to better understand the ... Abstract SY19-01: Cell to cell variability in the responses of tumor cells to death ligands ...http://cancerres.aacrjournals.org/content/70/8_Supplement/4110
Activation of VEGF and Ras genes in gastric mucosa during angiogenic response to ethanol injury | Gastrointestinal and Liver...
... completely blocked the induction of VEGF expression in cultured primary endothelial cells. Because, in other tissues, VEGF is ... Expression of VEGF mRNA is increased following ethanol-induced gastric mucosal injury.. VEGF mRNA expression after ethanol- ... bFGF was used to induce VEGF expression because bFGF has been shown to induce expression of VEGF in endothelial cells ... was used to quantify VEGF mRNA expression (Fig. 3,A-D). With this method, we determined the level of VEGF mRNA expression in ...http://ajpgi.physiology.org/content/276/6/G1345
Protocols and Video Articles Authored by Lixin Liu
Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy' ... Expression of mRNAs encoding the angiogenic cytokines vascular endothelial growth factor (VEGF), angiopoietin 1 (ANGPT1), ... mouse microvascular endothelial cells. Cultured human endothelial cells also stained positive for LSP1. Our results suggest ... HIF-1alpha mRNA levels in wound tissue were significantly reduced in older (4-6 months) as compared to younger (1.5-2 months) ...https://www.jove.com/author/Lixin_Liu
Cross‐Talk Between Hypoxia and the Tumour via Exosomes | IntechOpen
... show that increasingly subjecting cancer cells to a hypoxic stimulus results in a gradual increase in VEGF mRNA levels and VEGF ... and thus facilitates the delivery of oxygenated blood to the hypoxic tissue through the induction of vascular endothelial ... Thus, cells cultured under hypoxia or constitutive HIF‐1α expression promoted EMT, whereas the repression of TWIST expression ... Similar studies were carried out on different cell lines (e.g. leukaemia cell line, K562; human microvascular endothelial cells ...https://www.intechopen.com/books/hypoxia-and-human-diseases/cross-talk-between-hypoxia-and-the-tumour-via-exosomes/
Expression of HPA in astrocytomas and its effect on invasiveness of tumor cells
... , Yusong Bian, Li Guo, Weiguang Chen, Dan Sheng ... the expression and cell invasiveness change of tumor cells HPA, vascular endothelial growth factor (VEGF), matrix ... Realtime PCR results showed that the expression level of HPAmRNA in astrocytoma tissues was significantly higher than that in ... The influence of HPAsiRNA on the expression of VEGFmRNA in U87 cells and protein Realtime-PCR and Western blot results showed ...http://www.alliedacademies.org/articles/expression-of-hpa-in-astrocytomas-and-its-effect-on-invasiveness-of-tumor-cells-9566.html
Tumor suppressor ARF regulates tissue microenvironment and tumor growth through modulation of macrophage polarization
VEGFR-2 signaling is inhibited by TIMP3 via trapping VEGF and blockage of VE-cadherin expression on endothelial cells [23, 24 ... The mRNA levels of VEGF receptor-2 (VEGFR-2), vascular endothelial cadherin (VE-cadherin), and adhesion molecules as VCAM-1, ... An Endothelial Cell Genetic Screen Identifies the GTPase Rem2 as a Suppressor of p19ARF Expression That Promotes Endothelial ... been reported to regulate endothelial cell proliferation and angiogenesis and to modulate tumor vascularity via VEGF expression ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5341841/
Effects of total glucosides of peony on expression of VEGF mRNA in skin tissue and peripheral blood of psoriasis mice
... , Yaping ... Dvorak AM, Feng D. The vesiculo-vacuolar organelle (VVO). A new endothelial cell permeability organelle. J Histochem Cytochem ... Table 1: VEGF mRNA expression level in skin tissue.. Effect of TGP inhibition in expression of VEGF mRNA in Peripheral blood of ... The VEGF mRNA expression amounts of mice skin tissue and peripheral blood from model group are all higher than those from blank ...http://www.alliedacademies.org/articles/effects-of-total-glucosides-of-peony-on-expression-of-vegf-mrna-in-skin-tissue-and-peripheral-blood-of-psoriasis-mice.html