Rifampicin Prevents SH-SY5Y Cells from Rotenone-Induced Apoptosis via the PI3K/Akt/GSK-3β/CREB Signaling Pathway | SpringerLink
Rotenone-treated SH-SY5Y cells were used as the cell model to investigate the neuroprotective effects of rifampicin. Cell ... Both the cell viability and the expression of phospho-CREB in cells pretreated with rifampicin were higher than those of cells ... Jing X, Shi Q, Bi W, Zeng Z, Liang Y, Wu X, Xiao S, Liu J, Yang L, Tao E (2014) Rifampicin protects PC12 cells from rotenone- ... This study aims to investigate whether rifampicin protects rotenone-lesioned SH-SY5Y cells via regulating PI3K/Akt/GSK-3β/CREB ...https://link.springer.com/article/10.1007/s11064-018-2494-y
Basic Fibroblast Growth Factor Protects against Rotenone-Induced Dopaminergic Cell Death through Activation of Extracellular...
Rotenone induces apoptosis in SH-SY5Y cells in a concentration-dependent manner. To study mechanisms of rotenone-induced cell ... B, Reduced BAD expression renders SH-SY5Y cells resistant to rotenone. Cells were transfected with siRNA as in A. Cells were ... SH-SY5Y cells were pretreated with 10 ng/ml bFGF or vehicle control for 1 h, followed by 0 or 100 nm rotenone for 12 h. Cells ... by small interfering RNA rendered SH-SY5Y cells resistant to rotenone, implicating BAD in rotenone-induced cell death. ...http://www.jneurosci.org/content/26/17/4481
Novel Radiolytic Rotenone Derivative, Rotenoisin B with Potent Anti-Carcinogenic Activity in Hepatic Cancer Cells. | Sigma...
... similar to rotenone in hepatic cancer cells with low toxicity to normal cells even at high concentrations compared to rotenone. ... Novel Radiolytic Rotenone Derivative, Rotenoisin B with Potent Anti-Carcinogenic Activity in Hepatic Cancer Cells.. [Srilatha ... Interestingly, rotenoisin B showed low toxic effects on normal cells compared to rotenone. Mitochondrial transmembrane ... rotenone. Our results showed rotenoisin B inhibited hepatic cancer cells' proliferation in a dose dependent manner and ...https://www.sigmaaldrich.com/catalog/papers/26213921
Abstract 19986: Rotenone-Induced Mitochondrial Oxidative Stress Corrupts Tube Formations in Human Coronary Artery Endothelial...
Rotenone-Induced Mitochondrial Oxidative Stress Corrupts Tube Formations in Human Coronary Artery Endothelial Cells by ... Rotenone-Induced Mitochondrial Oxidative Stress Corrupts Tube Formations in Human Coronary Artery Endothelial Cells by ... Rotenone-Induced Mitochondrial Oxidative Stress Corrupts Tube Formations in Human Coronary Artery Endothelial Cells by ... Rotenone-Induced Mitochondrial Oxidative Stress Corrupts Tube Formations in Human Coronary Artery Endothelial Cells by ...http://circ.ahajournals.org/content/122/Suppl_21/A19986
... complex I inhibitor rotenone, cell death was dramatically increased, , mimicking the effects of a known parkinsonism-inducing ... neuromodulator in other cells (Jaffrey and Snyder, 1996), , and it can protect still other cells from known toxins (Wink et al ... AMPH also induced an early , burst of free radicals in a CNS-derived dopaminergic cell line. However, , AMPH-mediated ... Despite these AMPH-induced changes, neither protein oxidation nor cell death , was observed until 1 and 4 days, respectively. ...http://www.bio.net/bionet/mm/neur-sci/2003-May/054567.html
Preparing For Restoration - Yellowstone National Park (U.S. National Park Service)
Essentially, rotenone starves the cells, causing death.. To treat a section of stream, rotenone is dripped in at a rate ... Further downstream, potassium permanganate is added to the water to neutralize the rotenone. Rotenone is quickly broken down in ... Rotenone occurs in the roots, stems, and leaves of tropical plants in the pea family (Fabaceae). Ingestion has a relatively ... Rotenone must be absorbed into the bloodstream, usually across the gill membrane. It kills by inhibiting the biochemical ...https://www.nps.gov/yell/learn/fishrestore.htm
Sestrin 2 attenuates neonatal rat cardiomyocyte hypertrophy induced by phenylephrine via inhibiting ERK1/2, Molecular and...
Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation ... featured by increased hypertrophic marker ANP and cell surface area. During this process, ERK1/2 cascade was further activated ... featured by increased hypertrophic marker ANP and cell surface area. During this process, ERK1/2 cascade was further activated ...https://www.deepdyve.com/lp/springer_journal/sestrin-2-attenuates-neonatal-rat-cardiomyocyte-hypertrophy-induced-by-dfa69mhvMj
Glutathione S-Transferase Omega 1 Activity Is Sufficient to Suppress Neurodegeneration in a Drosophila Model of Parkinson...
... increased GST Pi expression protected cells from rotenone-induced neurotoxicity (46). However, the various roles of GSTs remain ... Cell 5, 45-53 [PMC free article] [PubMed]. 45. Willingham S., Outeiro T. F., DeVit M. J., Lindquist S. L., Muchowski P. J. ( ... Cell Stress Chaperones 10, 312-328 [PMC free article] [PubMed]. 26. Walters K. B., Grant P., Johnson D. L. (2009) Evolution of ... J. Cell Sci. 123, 3849-3855 [PubMed]. 38. Tew K. D., Townsend D. M. (2011) Regulatory functions of glutathione S-transferase P1 ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3307323/?lang=en-ca
Nuclear receptor related-1 protein - Wikipedia
"Enhancing Beta-Catenin Activity via GSK3beta Inhibition Protects PC12 Cells against Rotenone Toxicity through Nurr1 Induction ... Cell. 137 (1): 47-59. doi:10.1016/j.cell.2009.01.038. PMC 2754279 . PMID 19345186. Codina A, Benoit G, Gooch JT, Neuhaus D, ... To determine which cells are dopaminergic, experiments measured the enzyme tyrosine hydroxylase (TH), which is needed for ... This is further confirmed by studies showing that when forcing NURR1 expression in naïve precursor cells, there is complete ...https://en.wikipedia.org/wiki/Nuclear_receptor_related-1_protein
Omigapil - Wikipedia
In human neuroblastoma (PAJU) cells, omigapil can also prevent toxicity from rotenone and GAPDH overexpression. Omigapil has an ... Omigapil rescues in vitro PC12 cells from rotenone toxicity, β-amyloid toxicity, nutrition withdrawal, and lactacystin. ... While omigapil was able to prevent programmed cell death for high-risk cells and prevent deterioration of concomitant motor ... "S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding". Nat. Cell Biol. 7 (7): ...https://en.wikipedia.org/wiki/Omigapil
Mitochondrial function and morphology are impaired in parkin-mutant fibroblasts. - PubMed - NCBI
... in parkin mutant cells after rotenone exposure, ** p = 0.01. Similar shortening of the mitochondria is observed in cells with ... A) Rotenone treatment leads to a reduction of the mitochondrial membrane potential in control cells by 54%, * p , 0.05. There ... D) Mitochondrial branching (form factor) is increased in control and patient cells after rotenone exposure (* p , 0.05) to a ... Images of 25 individual cells were assessed per cell line per day and this was repeated on 3 separate occasions. Data is ...https://www.ncbi.nlm.nih.gov/pubmed/19067348?access_num=19067348&link_type=MED&dopt=Abstract
Molecules and Cells
A) Rotenone induces intracellular ROS production in SH-SY5Y cells. The indicated cells were treated with rotenone (15 μM) for 0 ... or necrotic cell death (PI+/Annexin V−). Rotenone induced cell death in 25.79% of SH-SY5Y cells (PI−/Annexin V+ or PI+/Annexin ... Treatment with rotenone (15 μM for 24 h) reduced cell viability by approximately 50% in SH-SY5Y cells (Fig. 1A). Pre-treatment ... For dose-response studies of rotenone toxicity, SH-SY5Y cells and primary cortical neurons were cultured with rotenone at 0- ...http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.2320
Browsing Department of Pathology Faculty Papers by Author "Leroueil, Marc"
Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells Jin, Jinghua; Davis, Jeanne; Zhu ... Rotenone, a specific inhibitor of mitochondrial complex ... ...https://digital.lib.washington.edu/researchworks/handle/1773/15671/browse?value=Leroueil%2C+Marc&type=author
Frontiers | Environmental pollutants as risk factors for neurodegenerative disorders: Alzheimer and Parkinson diseases |...
Radad, K., Rausch, W. D., and Gille, G. (2006). Rotenone induces cell death in primary dopaminergic culture by increasing ROS ... These effects were also observed in neuroblastoma SK-N-MC cells treated with rotenone (5 nM/4 weeks), as well as a loss of GSH ... and causes cell injury leading to apoptotic cell death (Berry et al., 2010; Franco et al., 2010). PQ has been used as a ... neuroblastoma cells to silica NPs (SiNPs) (10 µg/mL/24 h) raised the intracellular content of Aβ in both cell lines, which was ...https://www.frontiersin.org/articles/10.3389/fncel.2015.00124/full
STI571 protected neurons from rotenone-induced neuronal cell death. (ANOVA; p , 0.01, n = 3). B, Hippocampal neuron cultures [ ... The mechanisms leading to MST activation and cell death vary depending on stimulus specificity and cell type. Exposure of cells ... C, SH-SY5Y cells were left untreated or treated with 200 μm H2O2 for 1 h with or without 10 μm STI571. Lysates of cells were ... D, Neuro2A cells were transfected with c-Abl RNAi or control vector. At 72 h after transfection, cells were treated with 50 μg/ ...http://www.jneurosci.org/content/31/26/9611
Bee Venom Protects Against Neuron Cell Death
... Bee Venom Protects against Rotenone-Induced Cell Death in NSC34 Motor Neuron Cells ... NSC34 motor neuron cells were pretreated with 2.5 μg/mL BV and stimulated with 10 μM rotenone to induce cell toxicity. We ... related to cell death and increased ERK phosphorylation involved in cell survival in rotenone-treated NSC34 motor neuron cells. ... Pretreatment with 2.5 μg/mL BV had a neuroprotective effect against 10 μM rotenone-induced cell death in NSC34 motor neuron ...http://apitherapy.blogspot.com/2015/09/bee-venom-protects-against-neuron-cell.html
S-nitrosylation of UCHL1 induces its structural instability and promotes α-synuclein aggregation
SH-SY5Y cells were grown on a cover slip and cells were treated with rotenone for 24. h. UCHL1 in fixed cells was visualized by ... Rotenone treated cells showed increased cytoplasmic aggregation compared to untreated cells (40% in treated cells and 20% in ... SH-SY5Y cells were treated with rotenone (1. μM) for 16. h and processed for immunostaining. Briefly, cells were fixed in 4% ... Cells were treated with rotenone for 24. h and harvested cells were loaded on to gel filtration column. In another condition, ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5353675/
BV pretreatment prevents rotenone-induced cytotoxicity | Open-i
A) NSC34 cells were treated with 10 µM rotenone for 0, 3, 6, 12, and 24 h and ... BV pretreatment prevents rotenone-induced cytotoxicity in NSC34 neuronal cells. ( ... To examine the cytotoxicity of NSC34 cells after rotenone treatment, we incubated NSC34 motor neuron cells with 10 μM rotenone ... To examine the cytotoxicity of NSC34 cells after rotenone treatment, we incubated NSC34 motor neuron cells with 10 μM rotenone ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4591667_toxins-07-03715-g001&req=4
Azide - definition of azide by The Free Dictionary
Moreover, PQQ protected cells from mitochondrial inhibition by rotenone, 3-nitropropionic acid, antimycin A, and sodium azide. ...http://www.thefreedictionary.com/azide
Betaine is a positive regulator of mitochondrial respiration (Journal Article) | SciTech Connect
Its pretreatment can rescue cells exposed to toxins such as rotenone, chloroform, and LiCl. Furthermore, it has been suggested ... Abstract: Betaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways. It ... We propose that the anti-proliferative effects of betainemore » on cancer cells might be due to enhanced mitochondrial function ... LIVER CELLS; MEMBRANES; METABOLISM; MITOCHONDRIA; NEOPLASMS; NERVOUS SYSTEM DISEASES; OXIDASES; OXIDATION; PHOSPHORYLATION; ...https://www.osti.gov/scitech/biblio/22416893-betaine-positive-regulator-mitochondrial-respiration
Moreover, parkin rescues both mutant LRRK2 and rotenone-enhanced DA neuronal cell loss. This putative link between LRRK2, ... In rotenone-treated flies, flies were fed with cornmeal-agar medium containing 50 μm rotenone (Sigma) immediately at after ... Exposure to rotenone accelerates DA degeneration in LRRK2 G2019 and G2385R mutant flies. A, Bar graph showing the number of TH- ... Climbing assays and rotenone treatment.. Climbing assays were performed according to the method described previously (Wang et ...http://www.jneurosci.org/content/29/36/11257
OVCAR-3 cells were treated with DPI and rotenone. As showed in Fig. 3B , VEGF protein expression in the cells was significantly ... Reagents and cell culture. The human ovarian cancer cells OVCAR-3 and A2780 and immortalized ovarian surface epithelial cells ... To further test the ROS levels in other cancer cells, ovarian cancer cell lines A2780/CP70, SKOV-3, and prostate cancer cells ... 105 cells per well for 24 h. CM2-DCFHDA (5 μmol/L) was added into the cell culture medium and incubated for 30 min. The cells ...http://cancerres.aacrjournals.org/content/67/22/10823
Mitochondrial dysfunction remodels one-carbon metabolism in human cells | eLife
... we withdrew serine from cells treated with inhibitors. Antimycin- or rotenone-treated cells, but not control DMSO-treated cells ... Cell culture. T-REx-293 cells were obtained from Thermo Fisher (Waltham, MA). POLGdn cells were generated as in Jazayeri et al ... We failed to observe serine dependence in many other cell lines, including 293T cells which, like T-Rex293 and 293MSR cells, ... 3 d after the split, cells were trypsinized, and cells from replicate plates pooled and counted. 7×106 cells from each ...https://elifesciences.org/articles/10575?utm_source=content_alert&utm_medium=email&utm_content=fulltext&utm_campaign=elife-alerts
Most recent papers with the keyword Sirt1 AND Resveratrol | Read by QxMD
Here, we treated PC12 cells with rotenone to induce neurotoxicity, and the neurotoxic cells were subjected to resveratrol ... Rotenone is a common pesticide and has been reported as one of the risk factors for Parkinson disease. Rotenone can cause ... To investigate the mechanisms of this activity, we stimulated SW480 colon cancer cells with lipopolysaccharide, exposed cells ... Inhibiting autophagy is often applied as a new strategy to halt the growth of cancer cells. METHODS: The effect of FOXO1 gene ...https://www.readbyqxmd.com/keyword/71915
Frontiers | Degeneration of Dopaminergic Neurons Due to Metabolic Alterations and Parkinson's Disease | Frontiers in Aging...
Effects of insulin-like growth factor-1 on rotenone-induced apoptosis in human lymphocyte cells. Basic Clin. Pharmacol. Toxicol ... Glucose levels in culture medium determine cell death mode in MPP+-treated dopaminergic neuronal cells. Exp. Neurobiol. 24, 197 ... Insulin-like growth factor-I mediates neuroprotection in proteasome inhibition-induced cytotoxicity in SH-SY5Y cells. Mol. Cell ... Wang, L., Yang, H. J., Xia, Y. Y., and Feng, Z. W. (2010). Insulin-like growth factor 1 protects human neuroblastoma cells SH- ...https://www.frontiersin.org/articles/10.3389/fnagi.2016.00065/full