Herpes Simplex Virus Keratitis: A Treatment Guideline - 2014 - American Academy of Ophthalmology
7.3 days pre-inclusion and 20.9 +- 11.9 days post-inclusion. The study did not detect any significant change in T-cell subsets ... Studies examining the presence of HSV-1 DNA in the trigeminal ganglia have determined that at least 90% of the world's ... The measles virus suppresses cell mediated immunity by interfering with T-cell and dendritic cell function.54 Patients with an ... Typical epithelial lesions were defined as either punctate foci of opaque swollen epithelial cells that enlarge over 1-2 days ...https://www.aao.org/clinical-statement/herpes-simplex-virus-keratitis-treatment-guideline
Frontiers | The emergence of Pax7-expressing muscle stem cells during vertebrate head muscle development | Frontiers in Aging...
Currently, the in vitro manipulation and culture of these cells is still in its infancy, yet muscle stem cells may be the most ... Currently, the in vitro manipulation and culture of these cells is still in its infancy, yet muscle stem cells may be the most ... Unlike trunk muscle stem cells, head muscle stem cells do not have a previous history of Pax7 expression, instead Pax7 ... Unlike trunk muscle stem cells, head muscle stem cells do not have a previous history of Pax7 expression, instead Pax7 ...https://www.frontiersin.org/articles/10.3389/fnagi.2015.00062/full
Evaluation of the neuropeptide expression profile in the trigeminal ganglia after corneal herpes simplex virus 1 infection in...
Flow cytometry of NK1R KO mouse TGs at 5 day p.i showed an increased frequency of CD4 and CD8 T cells compared to B6 mice. ... infection leads to retrograde transport of the virus to the trigeminal ganglia (TG). As a result, immune cells infiltrate the ... Evaluation of the neuropeptide expression profile in the trigeminal ganglia after corneal herpes simplex virus 1 infection in ... Evaluation of the neuropeptide expression profile in the trigeminal ganglia after corneal herpes simplex virus 1 infection in ...http://iovs.arvojournals.org/article.aspx?articleid=2640891
Chromatolysis - Wikipedia
Examination of the trigeminal and dorsal root ganglia revealed peripheral chromatolysis in these cells. The cells exhibited ... for the 6 and 12 days groups respectively. For the purposes of the study A-cells were defined as ganglia neurons whose ... It is an induced response of the cell usually triggered by axotomy, ischemia, toxicity to the cell, cell exhaustion, virus ... and B-cell perikarya of their L5 dorsal root ganglion were examined. There was no morphological change in the B-cell perikarya ...https://en.wikipedia.org/wiki/Chromatolysis
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... the electrophysiological and immunohistochemical properties of acutely dissociated and 1-day cultured rat trigeminal ganglion ... In whole-cell patch-clamp recordings, the 1-day cultured neurons required a lower current threshold to induce an action ... Changes in expression of growth-associated protein-43 in trigeminal ganglion neurons and of the jaw opening reflex following ... The number of TRPV1-IR trigeminal ganglion (TG) neurons innervating the whisker pad skin was also increased significantly after ...https://www.jove.com/visualize?author=Suzuro%20Hitomi%20
Epitope specificity of CD8+ T cells in HSV-1 latently infected mouse trigeminal ganglia | IOVS | ARVO Journals
20% of HSV-specific CD8 T cells in day 14 TG but all of those in day 34 TG produced IFN-g. The CD8+ T cells that prevent HSV-1 ... RL Hendricks, KM Khanna; Epitope specificity of CD8+ T cells in HSV-1 latently infected mouse trigeminal ganglia . Invest. ... Epitope specificity of CD8+ T cells in HSV-1 latently infected mouse trigeminal ganglia ... Epitope specificity of CD8+ T cells in HSV-1 latently infected mouse trigeminal ganglia ...http://iovs.arvojournals.org/article.aspx?articleid=2421097
"Analysis of Bovine Herpesvirus 1 Transcripts during a Primary Infectio" by Luis M. Schang and Clinton J. Jones
Although the same cascade is assumed to occur during an infection of neurons in trigeminal ganglia (TG) of cattle, no ... IE transcripts were detected before E or L RNAs when rabbit kidney cells were infected with a low multiplicity of infection and ... Consequently, we analyzed BHV-1 gene expression in bovine TG at 1, 2, 4, 7, and 15 days postinfection (dpi). Infectious virus ... These studies suggested that expression of full-length and polyadenylated IE transcripts in trigeminal ganglia was not ...https://digitalcommons.unl.edu/vetscipapers/47/
In Herpetic Cornea, Inflammatory Cytokines Predominate, While Th1 Cytokines Hold Virus Spread in the Trigeminal Ganglion |...
Virus titer was maximum at 4 days PI in the cornea and at 5 days PI in the trigeminal ganglion. Stromal opacities appeared at 2 ... inflammatory cytokines were predominent and were responsible for cell infiltrations and corneal opacities. By contrast, Th1 ... while in the trigeminal ganglion, Th1 type cytokines (IL-2, IFN-gamma and IL-12) were preeminent at 4-8 days PI and 12 days PI ... The results were superimposed on the clinical scores and virus titers for corneas and trigeminal ganglia. Results: In the ...http://iovs.arvojournals.org/article.aspx?articleid=2417621
"Regulation of Neuropeptide Y, Vasoactive Intestinal Polypeptide, and T" by Debra D. DeLoach
Trigeminal ganglia were dissected after 1, 3, and 5 days after CFA-treatment. NPY expression was observed in Day 1 and Day 5 ... VIP expression was observed in Day 1 and Day 5 tissues, with localization to glial cells, and to both neurons and glial cell, ... and their receptors in mediating neuronal and glial cell activation in trigeminal ganglion that is likely to contribute to TMJ ... and to both neurons and glial cells, respectively. NPY receptor, NPY Y₂ was upregulated in Day 1 tissues, localized to neurons ...https://bearworks.missouristate.edu/theses/1262/
Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo
However, they failed to form neurons despite their presence in the condensing trigeminal ganglion. These results show that HFKs ... The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; ... into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 ... The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal ...https://scholarship.rice.edu/handle/1911/75339
Evidence that spontaneous reactivation of herpes virus does not occur in mice | Virology Journal | Full Text
Trigeminal ganglia removed from scid and immunocompetent recipient graft sites 5, 14, and 28 days after transplantation ... systemic viral infection and died within 14 days. Immunocompetent BALB/c mice that received ganglion grafts containing ... which survived the 65-day observation period with no evidence of viral infection although the immunocompetent mice developed ... spontaneous episodic production of immunogenic viral antigens but not of infectious virus occurs in mouse neural ganglia during ...https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-67
T-cell reconstitution during murine acquired immunodeficiency syndrome (MAIDS) produces neuroinflammation and mortality in...
... expression on CD4+ and CD8+ T-cells. Both T-cell loss and opportunistic brain infection were associated with high level PD-1 ... when T-cell immunity was reconstituted through adoptive transfer of virus-specific CD3+ T-cells, it resulted in significant ... HSV-infection of MAIDS mice stimulated peripheral immune cell infiltration into the brain and its ensuing microglial activation ... Experimental immune reconstitution disease of the CNS using T-cell repopulation of lymphopenic murine hosts harboring ...https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-10-98
Herpes labialis - Wikipedia
Inflammation (day 1): Virus begins reproducing and infecting cells at the end of the nerve. The healthy cells react to the ... the viruses move to sensory nerve ganglia (trigeminal ganglion), where they reside as lifelong, latent viruses. Asymptomatic ... Post-scab (12-14 days): A reddish area may linger at the site of viral infection as the destroyed cells are regenerated. Virus ... Pre-sore (day 2-3): This stage is defined by the appearance of tiny, hard, inflamed papules and vesicles that may itch and are ...https://en.wikipedia.org/wiki/Herpes_labialis
Functional crosstalk in culture between macrophages and trigeminal sensory neurons of a mouse genetic model of migraine | BMC...
Co-culturing WT or KI ganglia with host macrophages (active as much as resident cells) strongly stimulated single cell ... Trigeminal ganglion cultures from a genetic mouse model of migraine showed basal macrophage activation together with enhanced ... KI ganglion cultures constitutively contained a larger number of active macrophages, although no difference in P2X3 receptor ... investigated functional crosstalk between macrophages and sensory neurons in primary cultures from trigeminal sensory ganglia ...https://0-bmcneurosci-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2202-13-143
Role of M1 and M2 macrophages in herpes simplex virus-1 infectivity in vitro and in vivo | IOVS | ARVO Journals
Virus replication in the eye and the level of latency in the trigeminal ganglia (TG) of the infected mice were determined on ... Luminex assay was performed to measure the changes in the profile of cytokine/chemokine produced in the infected cells. In ... day 28 post infection. Results : Plaque assay results showed that HSV-1 infectivity was dramatically decreased in the M1 ...http://iovs.arvojournals.org/article.aspx?articleid=2640883
HSV, latency, and T cells
In early days, it was tentatively believed that (3) was not likely -- you'd need to have some signal to draw the immune ... 2007). Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia. Proc Natl ... In early days, it was tentatively believed that (3) was not likely - you'd need to have some signal to draw the immune response ... Chronic inflammation means there are immune cells hanging around inside the ganglia. What are these cells? Are they specific ...http://www.iayork.com/MysteryRays/2007/10/18/hsv-latency-and-t-cells/
Trigeminal ganglion. D, E, Petrosal ganglion. F, G, Geniculate ganglion. In each ganglion, a subpopulation of ganglion cells ... On the following day, color reaction was stopped by a 5 min incubation in Tris-EDTA buffer. Sections were mounted with glass ... Quantification of geniculate ganglion cell labeling.. Regions of interest in the perikaryon of individual ganglion cells (as ... 5-HT3A mRNA is expressed in ganglion cells. RT-PCR shows 5-HT3A transcripts in geniculate ganglia as well as in brain (Fig. 1A ...http://www.jneurosci.org/content/35/48/15984
Coiled-coil domain-containing 37 (FLJ40083) - Wikipedia
Between 20-30 days after birth in mus musculus, CCDC37 expression increases from less than 50% to about 85%. In rattus ... Most areas that express CCDC37 have an expression level of 20-40%. Expression levels in the trigeminal nerve, testis, medial ... Cell Biol. 107 (3): 841-9. Analysis and prediction of leucine-rich nuclear export signals Tanja la Cour, Lars Kiemer, Anne ... olfactory epithelium, dorsal root ganglia, and trachea are the highest with almost 75% expression. CCDC37 is expressed in the ...https://en.wikipedia.org/wiki/Coiled-coil_domain-containing_37_(FLJ40083)
Effects of CXCR3 Signaling on Development of Fatal Encephalitis and Corneal and Periocular Skin Disease in HSV-Infected Mice...
Cytokine and chemokine production in HSV-1 latently infected trigeminal ganglion cell cultures: effects of hyperthermic stress ... Corneas were collected on day 7 PI, digested, and stained for the cell surface markers CXCR3, CD11b, CD4, and Gr-1. Data are ... with no T cells present, 30 but higher cell yields were found for inflamed Tg and BSs of HSV-inoculated mice. Cell viability ... 2 3 Activated T cells, macrophages, natural killer (NK) cells and immature dendritic cells (DCs) that are functional for ...https://iovs.arvojournals.org/article.aspx?articleid=2125826
Kcnk18 - Potassium channel subfamily K member 18 - Mus musculus (Mouse) - Kcnk18 gene & protein
... trigeminal ganglion and dorsal root ganglia from E15.5 and increased through E18 to reach a peak in newborn mouse postnatal day ... Cell membrane 1 Publication. ,p>Manually curated information for which there is published experimental evidence.,/p> ,p>,a href ... High expression in trigeminal ganglion, also expressed in autonomic nervous system ganglia such as the stellate ganglion and ... Cell membrane, Membrane. ,p>This section provides information on the disease(s) and phenotype(s) associated with a protein.,p>, ...http://www.uniprot.org/uniprot/Q6VV64
EMMPRIN is Upregulated During Herpes Simplex Virus (HSV) Corneal Infection in vivo | IOVS | ARVO Journals
... by topical application of HSV strain K19 directly onto scratched corneas or by injection of virus into the trigeminal ganglion ... Signal was present in both epithelial and endothelial cells. Conclusion: This study demonstrates that EMMPRIN is expressed in ... Corneas were harvested 1 and 5 days post infection. EMMPRIN mRNA expression was assessed by in situ hybridization in the ... Staining was more intense in endothelial cells. Immunocytochemical staining for EMMPRIN revealed marked up-regulation of the ...https://iovs.arvojournals.org/article.aspx?articleid=2413210
Oral Manifestations of HIV
Following the primary episode, the virus becomes latent in the trigeminal ganglion. Recurrent oral herpes occurs at any age ... Cells are enlarged; some contain enlarged ballooning cells with pyknotic nuclei. Some contain perinuclear haloes. ... prednisone 40 to 60 mg/day for 7 to 10 days) has been reported as helpful.(82) The risks of steroid therapy, however, must be ... the lymphocytic infiltrate is predominantly CD8 cells, unlike that in Sjogren's syndrome, which is predominantly CD4 cells.(76 ...http://hivinsite.ucsf.edu/InSite?page=kb-04-01-14
Critical Role of MicroRNA-155 in Herpes Simplex Encephalitis | The Journal of Immunology
Preparation of trigeminal ganglia single-cell suspensions.. At 14 d after HSV-1 RE ocular infection, mice were anesthetized and ... PLNs were collected at day 5 p.i., and aliquots of single-cell suspensions were stained for cell-surface markers CD8 and Kb-gB ... CD8 T cells are required to contain HSV replication in ganglia, and they orchestrate this response largely by IFN-γ production ... One aliquot was left unmanipulated, and the other aliquot received 1 × 105 CD8 T cells isolated at day 8 p.i. from lymph nodes ...http://www.jimmunol.org/content/192/6/2734
Expression was highest at the interface between the ganglion cell layer and cytoblast layer. The ganglion cells were also Nogo- ... Nogo-A/B was also detected at high levels in peripheral ganglia other than DRG, e.g., the trigeminal ganglion (Fig. 3 C,D). ... The day of vaginal plug detection was considered embryonic day 1 (E1). Pregnant females were decapitated, and the embryos (E14 ... 7 C, inset). Once the granule cells had migrated past the Purkinje cells to their final position in the granule cell layer, ...http://www.jneurosci.org/content/22/9/3553
Canine herpesvirus - Wikipedia
The site of latency has been shown to be the trigeminal ganglion and possibly the lumbosacral ganglion. In adult dogs, the ... The incubation period of CHV is six to ten days. CHV is transmitted to puppies in the birth canal and by contact with infected ... The virus replicates in the surface cells of the nasal mucosa, tonsils, and pharynx. Low body temperature allows the virus to ... and death usually occurs in one to two days. In puppies three to five weeks old, the disease is less severe due to their ...https://en.wikipedia.org/wiki/Canine_herpesvirus