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*  Spidroin - Wikipedia
... spidroins have non-repetitive amino (N) and carboxyl (C) terminal domains of approximately 150 and 100 amino acids respectively ... Cell. Mol. Life Sci. (2011) 68:169-184 Scheller, J.; Guhrs, K.H.; Grosse, F.; Conrad, U. (2001). "Production of spider silk ... These alanine blocks can stack to create crystalline structures in the fiber, linking different protein molecules together. ... There are 100 tandem copies of 30 to 40 amino acids which repeat sequence and they represent more than 90% of the protein ...
  https://en.wikipedia.org/wiki/Spidroin
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Based on the natural sequence of Araneus diadematus Fibroin 4 (ADF4), the recombinant spider silk protein eADF4(C16) has been ... Chemistry, Issue 83, Geosciences (General), Exobiology, Miller-Urey, Prebiotic chemistry, amino acids, spark discharge ... Cell islands can be patterned by dispensing a cell-rich DEX phase into a PEG solution or by covering the DEX droplet with a ... Fibrous proteins display different sequences and structures that have been used for various applications in biomedical fields ...
  https://www.jove.com/visualize/abstract/20011532/tension-at-the-surface-which-phase-is-more-important-liquid-or-vapor
*  Patent US20040110439 - Native protein mimetic fibers, fiber networks and fabrics for medical use - Google Patents
Additionally, there may be living cells colonized on the material of the present invention or living cells may be incorporated ... fiber networks and nonwoven fabrics for medical use, with these materials characterized by good biocompatibility properties (e. ... This elastin mimetic protein contains 39 repeats ofthe amino acid sequence (Val-Pro-Gly-Val-Gly)4(Val-Pro-Gly-Lys-Gly) (SEQ ID ... have electrospun a series of silk-like protein polymers from formic acid and investigated their capacity to modify the cell ...
  http://www.google.co.uk/patents/US20040110439
*  US9109047B2 - High molecular ordered fibrilar structures, method for their preparation and uses thereof - Google...
... form dimers for directing ordered assembly of fibrous proteins such as silk proteins into super-molecular fibrilar structures. ... The invention further provides fibrilar structures such as fibers and articles comprising said fibrilar structures. ... The present invention relates to methods for generation of high molecular ordered fibrilar structures. More particularly, the ... sequence similarity at the amino acid level, with the synthetic repetitive units of the spider silk protein of the invention, ...
  https://patents.google.com/patent/US9109047B2/en
*  Properties of Engineered and Fabricated Silks | SpringerLink
Silk is a protein-based material which is predominantly produced by insects and spiders. Hundreds of millions of years of ... structural implications of a remarkable amino acid sequence. Proteins 44:119-122PubMedCrossRefGoogle Scholar ... Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science 295:472-476PubMedCrossRefGoogle ... Heim M, Keerl D, Scheibel T (2009) Spider silk: from soluble protein to extraordinary fiber. Angew Chem 48:3584-3596CrossRef ...
  https://link.springer.com/chapter/10.1007%2F978-3-319-49674-0_16
*  Difference Between Fibrous and Globular Protein - Pediaa.Com
Amino Acid Sequence. Furthermore, a fibrous protein consists of a repetitive amino acid sequence while a globular protein ... Other structures formed by fibrous proteins are silk and sponging.. What is a Globular Protein. A globular protein is a soluble ... 1. "Globular and Fibrous Proteins , Gcse-Revision, Biology, Cell-Activity, Proteins-and-Amino-Acids, Globular-and-Fibrous- ... Fibrous protein is a fiber-like protein with a helical or sheet structure. It mainly involves the formation of structures. On ...
  http://pediaa.com/difference-between-fibrous-and-globular-protein/
*  Modular Design in Natural and Biomimetic Soft Materials.
... the amino acid sequence of the protein precursor leading to the strongest and toughest silk fiber, that of major ampullate silk ... 2.2.1. Linear Elastin Mimicking Model Systems Once the primary repetitive amino acid sequence for tropoelastin was obtained, ... structures is that these synthetic sequences are often significantly shorter than those found in natural a-helical fibril/fiber ... achieved a significant breakthrough by using mammalian cells to produce high-mass, soluble silk protein polymer mimics in the ...
  https://www.docme.ru/doc/1938148/modular-design-in-natural-and-biomimetic-soft-materials
*  Randolf Lewis | Biology | USU
Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non-silk-like "spacer regions".. ... Aird, S.D, Kaiser, I.I, Lewis, R., Kruggel, W.G, (1986). A complete amino acid sequence for the basic subunit of crotoxin.. ... fiber mechanics, biocompatibility, and application in stem cell differentiation.. Biomacromolecules, 16:1, 202-13. ... Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks.. ...
  http://biology.usu.edu/about/faculty/randolph-lewis
*  Spider silk - Wikipedia
The primary structure is its amino acid sequence, mainly consisting of highly repetitive glycine and alanine blocks, which is ... Spider silk is a protein fibre spun by spiders. Spiders use their silk to make webs or other structures, which function as ... "Spider silk fibers spun from soluble recombinant silk produced in mammalian cells". Science. 295 (5554): 472-476. Bibcode: ... Nephila clavipes dragline silk is a two-protein fiber". J. Biol. Chem. 267 (27): 19320-19324. PMID 1527052. Simmons, A. H.; ...
  https://en.wikipedia.org/wiki/Spider_silk
*  Mass spider silk production through targeted gene replacement in Bombyx mori | PNAS
1986) Complete nucleotide sequence of the gene encoding the Bombyx mori silk protein P25 and predicted amino acid sequence of ... on integrating spider genes with different secondary structures and higher molecular weights containing repetitive structures. ... The silkworm silk fiber is the only natural fiber that can be produced on a large scale for commercial use. The silkworm silk ... 2002) Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science 295:472-476. ...
  http://www.pnas.org/content/115/35/8757.full
*  Protocols and Video Articles Authored by Ming Chen
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices' ... In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic ... causing a change in the amino acid sequence (Gly104â†'Arg). This amino acid alteration is predicted to disrupt a highly ... for Cell and Molecular Biology. Oct, 2011 , Pubmed ID: 22023480 Plants contain a large diversity of sphingolipid structures, ...
  https://www.jove.com/author/Ming_Chen
*  Patent US6620917 - Method for the purification and aqueous fiber spinning of spider silks and ... - Google Patents
Specifically, the lysis of bacteria and purification of silk proteins occur in a single solution of organic acid. Bacterial ... Silk proteins remain soluble as they are concentrated into a aqueous-based mixture for fiber spinning. ... proteins are hydrolyzed while the silk protein remains intact. ... in host cell strains that will maintain repetitive sequences in ... The resulting 30 amino acids of N-terminal sequence led to the identification of E. coli outer membrane protein, ompF. This ...
  http://www.google.com/patents/US6620917?dq=5,960,411
*  Spider silks: recombinant synthesis, assembly, spinning, and engineering of synthetic proteins | Microbial Cell Factories |...
Cloning strategy for constructing synthetic spider silk genes. (A) Amino acid sequences of designed silk modules were derived ... which combines synthetic repetitive silk sequences with authentic silk domains, reveals proteins that closely resemble silk ... based on amino acid composition, within the dragline fiber the molecular ratio between MaSp1 and MaSp2 and between ADF-4 and ... based on structures of comparable proteins [29-32]. If elasticity is due to GPGXX β-spirals, then this motif should be found in ...
  https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-3-14
*  Antheraea pernyi Silk Fiber: A Potential Resource for Artificially Biospinning Spider Dragline Silk
... and the similar amino acid composition between B. mori silk fibroin and spider dragline silk protein. ... Antheraea pernyi silk has more similar sequences to spider dragline silk than the silk from its domestic counterpart, Bombyx ... A. Lazaris, S. Arcidiacono, Y. Huang et al., "Spider silk fibers spun from soluble recombinant silk produced in mammalian cells ... pernyi silk fiber prepared by biomimetic spinning was also not found.. In this paper, the mechanical properties and structures ...
  https://www.hindawi.com/journals/bmri/2010/683962/
*  Protein-Based Hydrogels | SpringerLink
Arai KM, Takahashi R, Yokote Y, Akahane K (1983) Amino-acid sequence of feather keratin from fowl. Eur J Biochem 132(3):501-507 ... Minoura N, Aiba SI, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein ... Molecular and supramolecular structural studies on significant repetitive sequences of resilin. Chem BioChem 11(1):83-93Google ... Wood AT, Everett D, Budhwani KI, Dickinson B, Thomas V (2016) Wet-laid soy fiber reinforced hydrogel scaffold: fabrication ...
  https://link.springer.com/referenceworkentry/10.1007%2F978-3-319-76573-0_52-1
*  An ultra-high density bin-map for rapid QTL map...
The rapidly declining cost of genotyping due to recent advances in next-generation sequencing technologies has generated new ... starvation and lower rate of amino acid biosynthesis in the fl mutant ovules may impede the carbohydrate/energy supply and cell ... polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by ... To explore lint fiber initiation-related proteins in allotetraploid cotton (Gossypium hirsutum L.), a comparative proteomic ...
  https://www.scoop.it/t/plant-genomics/p/4022560934/2014/06/06/an-ultra-high-density-bin-map-for-rapid-qtl-mapping-for-tassel-and-ear-architecture-in-a-large-f2-maize-population
*  WO2013142901 SILK POLYPEPTIDES
... of the amino acids at position a are alanine residues and at least 20% of the amino acids at position d are aromatic residues. ... The present disclosure also provides nucleic acids encoding such polypeptides, and recombinant cells and/or organisms which ... wherein at least a portion of the polypeptide has a coiled coil structure comprising at least 10 copies of the heptad sequence ... The present disclosure provides polypeptides which encode a silk polypeptide, ...
  https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2013142901&recNum=1&maxRec=1000&office=&prevFilter=&sortOption=&queryString=&tab=PCTDescription
*  50-?? ?? ????????? ̳??????? ?????????? , ????????? e??? ???-??????? ?????? - ???????????
... alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA-R), and kainic acid (KA-R) (Kohl and Dannhardt 2001; Simeone et ... In the long run, the anastomo- sis was labeled with a silk sequence, in order to pro- tect the nervousness stock at the ever of ... In conflict to T-cells, B-lymphocytes (B-cells) are expert to in a little while realize pathogens result of the B-cell receptor ... Chemotherapy against Repetitive or Metastatic Cervical Cancer Women with widely metastatic and/or returning cervical can- cer ...
  http://politikan.com.ua/1/3/0/536217.htm
*  Plants and seeds of corn variety CV853963 - Monsanto Technology LLC
... modified fatty acid metabolism, abiotic stress resistance, altered seed amino acid composition, site-specific genetic ... Chem., 270:23044-23054, 1995); and the LOX sequence, used with CRE recombinase (Sauer, Mol. Cell. Biol., 7:2087-2096, 1987). ... Ear-Silk Color: The color of the silk observed 2 to 3 days after silk emergence scored as green-yellow, yellow, pink, red, or ... Biol., 21(3):415-428, 1993) and silicon carbide fiber-mediated transformation (U.S. Pat. No. 5,302,532 and U.S. Pat. No. ...
  http://www.freepatentsonline.com/9307721.html
*  Protocols and Video Articles Authored by Andrea Lim
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish' ... The C-terminus of DicA shows significant amino acid sequence similarity to the DNA-binding domains of RovA and SlyA, regulators ... Cell. Oct, 2012 , Pubmed ID: 23039994 How cells form global, self-organized structures using genetically encoded molecular ... With a longer fiber length in a CLS implementation a pulse energy of ~2.6 mJ is demonstrated with a corresponding M2 of ~4.2. ...
  https://www.jove.com/author/Andrea_Lim
*  Protocols and Video Articles Authored by Song Li (Translated to Hindi)
The deduced amino acid sequence showed the highest (42.8%) similarity to the α-amylase from Trichoderma viride. The RoAmy gene ... The Effect of Fiber Alignment and Heparin Coating on Cell Infiltration into Nanofibrous PLLA Scaffolds Biomaterials. May, 2010 ... area and inherent small pores of these structures give nanofibrous scaffolds high non-specific protein adsorption and poor cell ... In this study, we developed a protein resistant and porous nanofibrous scaffold composed of hyaluronan (HA), silk fibroin (SF ...
  https://www.jove.com/author/Song_Li?language=Hindi
*  Cross-Linkable Glycoproteins and Methods of Making the Same - Patent application
... comprising the amino acid sequence X-Hypn, wherein X is any amino acid, and n is from 1 to about 1000. 8. The non-naturally ... 0075]P3-type extensins play a crucial role in the cell wall, judging from their ubiquity and their highly conserved repetitive ... Spider silk proteins and methods for producing spider silk proteins. 2012-12-27. Soy whey protein compositions and methods for ... 0199]4. Fiber formation and Fiber properties-- [0200]Extrusion of crosslinked products through an appropriately sized nozzle ...
  http://www.patentsencyclopedia.com/app/20080262198
*  US8535388B2 - Bone graft - Google Patents
Open cell tantalum structures for cancellous bone implants and cell and tissue receptors ... show two longitudinal sections of allograft fiber material after 3 and 6 weeks of culturing with cells according to the state ... Multilayered silk scaffolds for meniscus tissue engineering. Bjerre et al. 2008. Flow perfusion culture of human mesenchymal ... In brief, tissue preferably is homogenized in 0.5 M acetic acid, the pH adjusted to 2.5 with HCl and the preparation digested ...
  https://patents.google.com/patent/US8535388B2/en
*  Institutionen der Universität Bayreuth Fakultäten | Fakultät für Ingenieurwissenschaften | Lehrstuhl Biomaterialien - ERef...
Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for ... Hierarchical structures made of proteins. The complex architecture of spider webs and their constituent silk proteins. In: ... The elaborate structure of spider silk : Structure and function of a natural high performance fiber. In: Prion. Bd. 2 (Oktober ... Resonance assignment of an engineered amino-terminal domain of a major ampullate spider silk with neutralized charge cluster. ...
  https://eref.uni-bayreuth.de/view/divisions/160013.html
*  Tol1 FACTOR TRANSPOSASE AND DNA INTRODUCTION SYSTEM USING THE SAME - Patent application
... the amino acid sequence of SEQ ID No: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence of ... activity in vertebrate cells. Nucleic Acids Res 31: 6873-6881 [0260]15. O'Hare K., Rubin G. M. (1983) Structures of P ... rearrangement within the Antheraea pernyi silk fibroin gene is associated with four types of repetitive units. J. Mol. Evol. 51 ... fiber protein (specific infection vector) and a gutted vector from which improvement in an expression efficiency of a desired ...
  http://www.patentsencyclopedia.com/app/20100129914